Restriction map for
pFN21AB6402
(Linear) (Six-base) MAPSORT of: KIBB6402.seq Check: 4079 from: 1 to: 999
KIBB6402.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 258 999
Size: 258 741
AccI GT'mk_AC
Cuts at: 0 500 999
Size: 500 499
AlwNI CAG_nnn'CTG
Cuts at: 0 495 999
Size: 495 504
ApaI G_GGCC'C
Cuts at: 0 731 999
Size: 731 268
ApoI r'AATT_y
Cuts at: 0 259 999
Size: 259 740
BanII G_rGCy'C
Cuts at: 0 731 999
Size: 731 268
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 999
Size: 821 178
BbvCI CC'TCA_GC
Cuts at: 0 45 975 999
Size: 45 930 24
Fragments arranged by size:
930 45 24
BclI T'GATC_A
Cuts at: 0 63 999
Size: 63 936
BglII A'GATC_T
Cuts at: 0 346 999
Size: 346 653
Bme1580I G_kGCm'C
Cuts at: 0 731 999
Size: 731 268
BmrI ACTGGGnnnn_n'
Cuts at: 0 473 999
Size: 473 526
Bpu10I CC'TnA_GC
Cuts at: 0 45 686 975 999
Size: 45 641 289 24
Fragments arranged by size:
641 289 45 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 999
Size: 500 499
BsaAI yAC'GTr
Cuts at: 0 375 933 999
Size: 375 558 66
Fragments arranged by size:
558 375 66
BsaWI w'CCGG_w
Cuts at: 0 455 950 999
Size: 455 495 49
Fragments arranged by size:
495 455 49
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 999
Size: 156 843
BseYI C'CCAG_C
Cuts at: 0 902 999
Size: 902 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 999
Size: 166 833
BsiHKAI G_wGCw'C
Cuts at: 0 438 999
Size: 438 561
Bsp1286I G_dGCh'C
Cuts at: 0 438 731 999
Size: 438 293 268
Fragments arranged by size:
438 293 268
BspEI T'CCGG_A
Cuts at: 0 455 999
Size: 455 544
BspHI T'CATG_A
Cuts at: 0 756 999
Size: 756 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 258 742 999
Size: 258 484 257
Fragments arranged by size:
484 258 257
BsrDI GCAATG_nn'
Cuts at: 0 528 540 999
Size: 528 12 459
Fragments arranged by size:
528 459 12
BsrFI r'CCGG_y
Cuts at: 0 667 999
Size: 667 332
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 999
Size: 351 648
BstYI r'GATC_y
Cuts at: 0 346 999
Size: 346 653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 115 999
Size: 115 884
BtsI GCAGTG_nn'
Cuts at: 0 155 816 999
Size: 155 661 183
Fragments arranged by size:
661 183 155
DraIII CAC_nnn'GTG
Cuts at: 0 484 999
Size: 484 515
EaeI y'GGCC_r
Cuts at: 0 224 941 999
Size: 224 717 58
Fragments arranged by size:
717 224 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 706 999
Size: 706 293
EcoO109I rG'GnC_Cy
Cuts at: 0 339 699 727 999
Size: 339 360 28 272
Fragments arranged by size:
360 339 272 28
EcoRI G'AATT_C
Cuts at: 0 259 999
Size: 259 740
MscI TGG'CCA
Cuts at: 0 226 943 999
Size: 226 717 56
Fragments arranged by size:
717 226 56
MslI CAynn'nnrTG
Cuts at: 0 169 999
Size: 169 830
MspA1I CmG'CkG
Cuts at: 0 902 999
Size: 902 97
NdeI CA'TA_TG
Cuts at: 0 609 999
Size: 609 390
PfoI T'CCnGG_A
Cuts at: 0 317 999
Size: 317 682
PspOMI G'GGCC_C
Cuts at: 0 727 999
Size: 727 272
PvuII CAG'CTG
Cuts at: 0 902 999
Size: 902 97
SfcI C'TryA_G
Cuts at: 0 804 999
Size: 804 195
SmlI C'TyrA_G
Cuts at: 0 515 999
Size: 515 484
SnaBI TAC'GTA
Cuts at: 0 933 999
Size: 933 66
SspI AAT'ATT
Cuts at: 0 883 999
Size: 883 116
StuI AGG'CCT
Cuts at: 0 584 999
Size: 584 415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 999
Size: 913 86
TatI w'GTAC_w
Cuts at: 0 635 999
Size: 635 364
XmnI GAAnn'nnTTC
Cuts at: 0 313 999
Size: 313 686
Enzymes that do cut:
AarI AccI AlwNI ApaI ApoI BanII BbsI BbvCI
BclI BglII Bme1580I BmrI Bpu10I BpuEI BsaAI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BstXI BstYI BtgZI BtsI DraIII EaeI
EcoNI EcoO109I EcoRI MscI MslI MspA1I NdeI PfoI
PspOMI PvuII SfcI SmlI SnaBI SspI StuI TaqII
TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BlpI BmgBI BmtI BplI BpmI BsaI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI Eco57MI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI