################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DpnII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HpaI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MroI MspA1I MunI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 202 CGCG CG^CG AcsI 206 AAATTC [GA]^AATT[CT] AcyI 350 GACGCC G[GA]^CG[CT]C AlwNI 622 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 206 AAATTC [GA]^AATT[CT] AvaI 256 CCCGGG C^[CT]CG[GA]G AvaII 715 GGTCC G^G[AT]CC BbvCI 517 GCTGAGG CCTCAGC(-5/-2) BsaHI 350 GACGCC G[GA]^CG[CT]C BsiEI 669 CGACCG CG[GA][CT]^CG BsoBI 256 CCCGGG C^[CT]CG[GA]G BspDI 190 ATCGAT AT^CGAT BspMI 560 ACCTGC ACCTGC(4/8) BsrDI 312 CATTGC GCAATG(2/0) BstUI 202 CGCG CG^CG BstXI 380 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 201 GCGC GCG^C ClaI 190 ATCGAT AT^CGAT DraI 406 TTTAAA TTT^AAA DraII 650 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoNI 654 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 650 AGGGCCT [GA]G^G[ACGT]CC[CT] HgaI 350 GACGC GACGC(5/10) HhaI 201 GCGC GCG^C Hin1I 350 GACGCC G[GA]^CG[CT]C HinP1I 201 GCGC G^CGC HincII 487 GTTGAC GT[CT]^[GA]AC HindII 487 GTTGAC GT[CT]^[GA]AC HindIII 283 AAGCTT A^AGCTT MseI 407 TTAA T^TAA MslI 171 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 202 CGCG CG^CG PflMI 621 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SexAI 620 ACCAGGT A^CC[AT]GGT SfaNI 193 GATGC GCATC(5/9) SmaI 256 CCCGGG CCC^GGG SmlI 469 CTCAAG C^T[CT][GA]AG TaqI 191 TCGA T^CGA TfiI 303 GAATC G^A[AT]TC Tru9I 407 TTAA T^TAA Tsp509I 207 AATT ^AATT TspRI 10 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 191 TCGA T^CGA Van91I 621 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 440 CCAAGAAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 256 CCCGGG C^CCGGG XmaI 256 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 447 GTAC GT^AC 666 GTAC Asp700I 99 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 282 GAAGCTTTTC AspHI 268 GAGCAC G[AT]GC[AT]^C 685 GAGCAC BalI 584 TGGCCA TGG^CCA 629 TGGCCA BfaI 428 CTAG C^TAG 604 CTAG BpmI 244 CTCCAG CTGGAG(16/14) 679 CTGGAG BseRI 454 GAGGAG GAGGAG(10/8) 682 GAGGAG BsiHKAI 268 GAGCAC G[AT]GC[AT]^C 685 GAGCAC BsmFI 111 GTCCC GGGAC(10/14) 716 GTCCC BsmI 513 GAATGC GAATGC(1/-1) 68 GCATTC BstF5I 171 CATCC GGATG(2/0) 504 GGATG BstNI 354 CCTGG CC^[AT]GG 621 CCAGG DsaI 263 CCATGG C^C[GA][CT]GG 719 CCATGG EarI 535 GAAGAG CTCTTC(1/4) 733 GAAGAG EcoRII 354 CCTGG ^CC[AT]GG 621 CCAGG FokI 171 CATCC GGATG(9/13) 504 GGATG HapII 114 CCGG C^CGG 257 CCGG HpaII 114 CCGG C^CGG 257 CCGG Ksp632I 535 GAAGAG CTCTTC(1/4) 733 GAAGAG MaeI 428 CTAG C^TAG 604 CTAG MaeIII 226 GTGAC ^GT[ACGT]AC 358 GTGAC MluNI 584 TGGCCA TGG^CCA 629 TGGCCA MscI 584 TGGCCA TGG^CCA 629 TGGCCA MspI 114 CCGG C^CGG 257 CCGG MvaI 354 CCTGG CC^[AT]GG 621 CCAGG NcoI 263 CCATGG C^CATGG 719 CCATGG PleI 360 GACTC GAGTC(4/5) 3 GAGTC RsaI 447 GTAC GT^AC 666 GTAC Tsp45I 226 GTGAC ^GT[GC]AC 358 GTGAC XmnI 99 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 282 GAAGCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI Cac8I Cfr13I DdeI EaeI EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MboII MnlI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI StyI TseI