(Circular) (Six-base) MAPSORT of: pFN21AE0132.seq Check: 5588 from: 1 to: 5443 pFN21AE0132 5443 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3253 278 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 1937 Size: 849 4594 Acc65I G'GTAC_C Cuts at: 2764 2764 Size: 5443 AclI AA'CG_TT Cuts at: 1563 3570 3943 1563 Size: 2007 373 3063 Fragments arranged by size: 3063 2007 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 3506 4890 5158 1632 Size: 944 930 1384 268 1917 Fragments arranged by size: 1917 1384 944 930 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5246 AflIII A'CryG_T Cuts at: 1203 4342 5319 1203 Size: 3139 977 1327 Fragments arranged by size: 3139 1327 977 AgeI A'CCGG_T Cuts at: 4274 4274 Size: 5443 AhdI GACnn_n'nnGTC Cuts at: 4172 4172 Size: 5443 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2037 1184 Size: 853 4590 AlwNI CAG_nnn'CTG Cuts at: 2632 3186 4758 2632 Size: 554 1572 3317 Fragments arranged by size: 3317 1572 554 ApaLI G'TGCA_C Cuts at: 3500 4656 3500 Size: 1156 4287 ApoI r'AATT_y Cuts at: 1493 2211 2752 2978 3130 5239 1493 Size: 718 541 226 152 2109 1697 Fragments arranged by size: 2109 1697 718 541 226 152 AseI AT'TA_AT Cuts at: 160 1032 3997 160 Size: 872 2965 1606 Fragments arranged by size: 2965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 AvaI C'yCGr_G Cuts at: 1870 1943 2261 2768 1870 Size: 73 318 507 4545 Fragments arranged by size: 4545 507 318 73 BamHI G'GATC_C Cuts at: 2773 3140 2773 Size: 367 5076 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2764 4219 618 Size: 325 217 627 977 1455 1842 Fragments arranged by size: 1842 1455 977 627 325 217 BanII G_rGCy'C Cuts at: 1391 2762 1391 Size: 1371 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4472 BbvCI CC'TCA_GC Cuts at: 2523 2523 Size: 5443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3634 3668 1903 Size: 34 1697 34 3678 Fragments arranged by size: 3678 1697 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 4545 3335 Size: 1210 4233 BclI T'GATC_A Cuts at: 1548 1548 Size: 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4054 136 Size: 107 122 71 631 605 2382 1525 Fragments arranged by size: 2382 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5438 5438 Size: 5443 BlpI GC'TnA_GC Cuts at: 2860 2860 Size: 5443 Bme1580I G_kGCm'C Cuts at: 1790 2302 3504 4660 1790 Size: 512 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 512 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4132 442 Size: 416 53 83 399 24 1757 958 1753 Fragments arranged by size: 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2705 4103 1239 Size: 995 471 1398 2579 Fragments arranged by size: 2579 1398 995 471 Bpu10I CC'TnA_GC Cuts at: 2523 2523 Size: 5443 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 3568 4433 4731 2459 Size: 1109 865 298 3171 Fragments arranged by size: 3171 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4106 882 Size: 617 2607 2219 Fragments arranged by size: 2607 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3234 493 Size: 711 2030 2702 Fragments arranged by size: 2702 2030 711 BsaBI GATnn'nnATC Cuts at: 5437 5437 Size: 5443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2356 3250 3632 4267 Size: 53 83 186 936 823 894 382 635 Cuts at: 4267 275 Size: 1451 Fragments arranged by size: 1451 936 894 823 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3876 4274 4548 4695 5106 3876 Size: 398 274 147 411 4213 Fragments arranged by size: 4213 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2475 2505 1178 Size: 30 165 30 1072 30 4116 Fragments arranged by size: 4116 1072 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 2702 1186 Size: 1288 228 3927 Fragments arranged by size: 3927 1288 228 BseYI C'CCAG_C Cuts at: 4646 5142 4646 Size: 496 4947 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4870 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2677 2893 3654 4682 1540 Size: 275 186 676 216 761 1028 2301 Fragments arranged by size: 2301 1028 761 676 275 216 186 BsiHKAI G_wGCw'C Cuts at: 2277 2694 3504 3589 4660 2277 Size: 417 810 85 1071 3060 Fragments arranged by size: 3060 1071 810 417 85 BsmI GAATG_Cn' Cuts at: 2073 2524 2954 3047 2073 Size: 451 430 93 4469 Fragments arranged by size: 4469 451 430 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2302 2694 2762 3504 3589 4660 Size: 399 487 25 392 68 742 85 1071 Cuts at: 4660 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 487 399 392 85 68 25 BspEI T'CCGG_A Cuts at: 5106 5106 Size: 5443 BspHI T'CATG_A Cuts at: 3332 5062 3332 Size: 1730 3713 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2574 2798 844 Size: 1730 224 3489 Fragments arranged by size: 3489 1730 224 BsrBI CCG'CTC Cuts at: 1394 1823 3330 1394 Size: 429 1507 3507 Fragments arranged by size: 3507 1507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2316 3938 4112 65 Size: 1183 1068 1622 174 1396 Fragments arranged by size: 1622 1396 1183 1068 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4087 4274 1700 Size: 110 2277 187 2869 Fragments arranged by size: 2869 2277 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5443 BssSI C'ACGA_G Cuts at: 3503 4515 3503 Size: 1012 4431 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5443 BstBI TT'CG_AA Cuts at: 4247 4247 Size: 5443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5443 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2392 Size: 5443 BstYI r'GATC_y Cuts at: 1972 2773 3140 3525 3542 4994 5080 5092 5438 Size: 801 367 385 17 1452 86 12 346 Cuts at: 5438 1972 Size: 1977 Fragments arranged by size: 1977 1452 801 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2268 2724 513 Size: 552 1203 456 3232 Fragments arranged by size: 3232 1203 552 456 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4704 Fragments arranged by size: 4704 683 56 BtsI GCAGTG_nn' Cuts at: 2021 2959 3753 3773 2021 Size: 938 794 20 3691 Fragments arranged by size: 3691 938 794 20 ClaI AT'CG_AT Cuts at: 1554 2196 1554 Size: 642 4801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5408 DraI TTT'AAA Cuts at: 2413 2747 2413 Size: 334 5109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4450 809 Size: 507 3134 1802 Fragments arranged by size: 3134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2589 2634 Size: 54 1425 50 275 18 612 147 45 Cuts at: 2634 2890 3779 5408 8 Size: 256 889 1629 43 Fragments arranged by size: 1629 1425 889 612 275 256 147 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2890 1537 Size: 275 1078 4090 Fragments arranged by size: 4090 1078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2535 2733 3373 1353 Size: 1182 198 640 3423 Fragments arranged by size: 3423 1182 640 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2438 4030 4404 4550 387 Size: 1064 138 849 1592 374 146 1280 Fragments arranged by size: 1592 1280 1064 849 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2576 2705 3506 4103 4890 5158 Size: 393 602 342 129 801 597 787 268 Cuts at: 5158 1239 Size: 1524 Fragments arranged by size: 1524 801 787 602 597 393 342 268 129 EcoNI CCTnn'n_nnAGG Cuts at: 2663 2663 Size: 5443 EcoO109I rG'GnC_Cy Cuts at: 2656 2656 Size: 5443 EcoRI G'AATT_C Cuts at: 2752 3130 2752 Size: 378 5065 FspI TGC'GCA Cuts at: 3949 3949 Size: 5443 HaeII r_GCGC'y Cuts at: 1678 4590 1678 Size: 2912 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373 Size: 32 135 32 2512 32 42 32 2626 Fragments arranged by size: 2626 2512 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2494 2787 3033 669 Size: 988 281 556 293 246 3079 Fragments arranged by size: 3079 988 556 293 281 246 HindIII A'AGCT_T Cuts at: 748 2288 748 Size: 1540 3903 HpaI GTT'AAC Cuts at: 3033 3033 Size: 5443 KpnI G_GTAC'C Cuts at: 2768 2768 Size: 5443 MfeI C'AATT_G Cuts at: 3042 3042 Size: 5443 MluI A'CGCG_T Cuts at: 5319 5319 Size: 5443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 2514 4557 4741 1305 Size: 1182 27 2043 184 2007 Fragments arranged by size: 2043 2007 1182 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 2636 10 Size: 54 1425 343 759 45 2817 Fragments arranged by size: 2817 1425 759 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2180 3401 3760 3919 518 Size: 648 1014 1221 359 159 2042 Fragments arranged by size: 2042 1221 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2859 3536 4684 4929 1606 Size: 1253 677 1148 245 2120 Fragments arranged by size: 2120 1253 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2268 2724 513 Size: 552 1203 456 3232 Fragments arranged by size: 3232 1203 552 456 NdeI CA'TA_TG Cuts at: 387 387 Size: 5443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5443 NotI GC'GGCC_GC Cuts at: 2890 2890 Size: 5443 NspI r_CATG'y Cuts at: 1132 1229 2801 4346 1132 Size: 97 1572 1545 2229 Fragments arranged by size: 2229 1572 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5443 PciI A'CATG_T Cuts at: 4342 4342 Size: 5443 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 1108 Size: 1524 3919 PmeI GTTT'AAAC Cuts at: 2747 2747 Size: 5443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 5025 5057 3512 Size: 32 1481 32 3898 Fragments arranged by size: 3898 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5443 PsiI TTA'TAA Cuts at: 3013 3013 Size: 5443 PstI C_TGCA'G Cuts at: 830 2016 2026 2571 2706 2713 2795 830 Size: 1186 10 545 135 7 82 3478 Fragments arranged by size: 3478 1186 545 135 82 10 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5443 SalI G'TCGA_C Cuts at: 1936 2785 1936 Size: 849 4594 SbfI CC_TGCA'GG Cuts at: 2795 2795 Size: 5443 ScaI AGT'ACT Cuts at: 3691 5403 3691 Size: 1712 3731 SexAI A'CCwGG_T Cuts at: 2625 2625 Size: 5443 SfcI C'TryA_G Cuts at: 826 1045 2012 2022 2567 2702 2709 2791 3926 Size: 219 967 10 545 135 7 82 1135 Cuts at: 3926 4607 4798 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 967 681 545 219 191 135 82 10 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5443 SmaI CCC'GGG Cuts at: 1872 2263 2770 1872 Size: 391 507 4545 Fragments arranged by size: 4545 507 391 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 3547 4448 4710 820 Size: 197 926 531 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 531 262 197 SnaBI TAC'GTA Cuts at: 493 3234 493 Size: 2741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5443 SphI G_CATG'C Cuts at: 1132 2801 1132 Size: 1669 3774 SspI AAT'ATT Cuts at: 5 52 3367 5 Size: 47 3315 2081 Fragments arranged by size: 3315 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5443 StyI C'CwwG_G Cuts at: 513 1065 2268 2664 2724 2882 513 Size: 552 1203 396 60 158 3074 Fragments arranged by size: 3074 1203 552 396 158 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3470 3487 3640 3825 4270 325 Size: 1255 1890 17 153 185 445 1498 Fragments arranged by size: 1890 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 3689 5401 Size: 274 80 33 51 1443 474 1238 1712 Cuts at: 5401 96 Size: 138 Fragments arranged by size: 1712 1443 1238 474 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5403 5435 5403 Size: 32 5411 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5443 XbaI T'CTAG_A Cuts at: 2779 2779 Size: 5443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2452 2452 Size: 5443 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5443 XmaI C'CCGG_G Cuts at: 1870 2261 2768 1870 Size: 391 507 4545 Fragments arranged by size: 4545 507 391 XmnI GAAnn'nnTTC Cuts at: 2108 2291 3572 2108 Size: 183 1281 3979 Fragments arranged by size: 3979 1281 183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3251 276 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI