Restriction map for
pFN21AB7794
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BbeI
BbrPI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI MaeII MamI MfeI MluI
MseI MunI NarI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I TthHB8I XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1240 GTCTAC GT^[AC][GT]AC
AccIII 881 TCCGGA T^CCGGA
AfaI 1440 GTAC GT^AC
ApaI 1530 GGGCCC GGGCC^C
Asp700I 570 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 99 TGCGCA TGC^GCA
BalI 458 TGGCCA TGG^CCA
BamHI 46 GGATCC G^GATCC
BanI 1477 GGCACC G^G[CT][GA]CC
BbsI 1216 GAAGAC GAAGAC(2/6)
BfaI 952 CTAG C^TAG
BpuAI 1216 GAAGAC GAAGAC(2/6)
BseAI 881 TCCGGA T^CCGGA
BsgI 604 GTGCAG GTGCAG(16/14)
BsmI 1020 GAATGC GAATGC(1/-1)
BspEI 881 TCCGGA T^CCGGA
BsrFI 1371 GCCGGC [GA]^CCGG[CT]
BssHII 703 GCGCGC G^CGCGC
BstEII 1068 GGTCACC G^GT[ACGT]ACC
BstPI 1068 GGTCACC G^GT[ACGT]ACC
Cfr10I 1371 GCCGGC [GA]^CCGG[CT]
EcoO65I 1068 GGTCACC G^GT[ACGT]ACC
FspI 99 TGCGCA TGC^GCA
KspI 446 CCGCGG CCGC^GG
MaeI 952 CTAG C^TAG
MluNI 458 TGGCCA TGG^CCA
MroI 881 TCCGGA T^CCGGA
MscI 458 TGGCCA TGG^CCA
NaeI 1371 GCCGGC GCC^GGC
NgoMIV 1371 GCCGGC G^CCGGC
NotI 448 GCGGCCGC GC^GGCCGC
PshAI 1062 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1440 GTAC GT^AC
SacII 446 CCGCGG CCGC^GG
SapI 198 GAAGAGC GCTCTTC(1/4)
SexAI 599 ACCTGGT A^CC[AT]GGT
SfiI 450 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp509I 755 AATT ^AATT
XbaI 951 TCTAGA T^CTAGA
XcmI 464 CCACCCCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 570 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccII 447 CGCG CG^CG
704 CGCG
AlwNI 1042 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1060 CAGACTCTG
AspI 4 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1234 GACGGCGTC
BbvCI 480 CCTCAGC CCTCAGC(-5/-2)
814 GCTGAGG
BclI 545 TGATCA T^GATCA
1407 TGATCA
BglI 427 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
451 GCCGCCATGGC
BglII 902 AGATCT A^GATCT
1079 AGATCT
BsaI 1009 GAGACC GGTCTC(1/5)
1429 GAGACC
BsaWI 591 ACCGGA [AT]^CCGG[AT]
881 TCCGGA
BsiEI 26 CGGCCG CG[GA][CT]^CG
449 CGGCCG
BspMI 1120 ACCTGC ACCTGC(4/8)
1397 ACCTGC
BsrBI 666 CCGCTC CCGCTC(-3/-3)
270 GAGCGG
BssSI 1426 CACGAG CACGAG(-5/-1)
1168 CTCGTG
BstUI 447 CGCG CG^CG
704 CGCG
EagI 26 CGGCCG C^GGCCG
449 CGGCCG
EarI 198 GAAGAG CTCTTC(1/4)
873 GAAGAG
EclXI 26 CGGCCG C^GGCCG
449 CGGCCG
Eco52I 26 CGGCCG C^GGCCG
449 CGGCCG
FauI 468 CCCGC CCCGC(4/6)
665 CCCGC
FbaI 545 TGATCA T^GATCA
1407 TGATCA
HaeII 282 AGCGCC [GA]GCGC^[CT]
351 GGCGCT
Ksp632I 198 GAAGAG CTCTTC(1/4)
873 GAAGAG
MvnI 447 CGCG CG^CG
704 CGCG
PflFI 4 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1234 GACGGCGTC
PflMI 1059 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1502 CCAAGGAGTGG
PstI 1048 CTGCAG CTGCA^G
1399 CTGCAG
PvuII 535 CAGCTG CAG^CTG
1045 CAGCTG
SfcI 1048 CTGCAG C^T[GA][CT]AG
1399 CTGCAG
StuI 866 AGGCCT AGG^CCT
1451 AGGCCT
Tth111I 4 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1234 GACGGCGTC
Van91I 1059 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1502 CCAAGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI SmaI StyI
TseI Tsp45I TspRI XhoII XmaCI
XmaI