(Circular) (Six-base) MAPSORT of: pFN21AB7794.seq Check: 8204 from: 1 to: 6262 pFN21AB7794 6262 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3411 3411 Size: 6262 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3246 3605 1937 Size: 1309 359 4594 Fragments arranged by size: 4594 1309 359 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4325 5709 5977 1632 Size: 2693 1384 268 1917 Fragments arranged by size: 2693 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 5161 6138 1203 Size: 3958 977 1327 Fragments arranged by size: 3958 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3163 3344 3347 1184 Size: 1979 181 3 4099 Fragments arranged by size: 4099 1979 181 3 AlwNI CAG_nnn'CTG Cuts at: 3052 3070 4005 5577 3052 Size: 18 935 1572 3737 Fragments arranged by size: 3737 1572 935 18 ApaI G_GGCC'C Cuts at: 3539 3539 Size: 6262 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 3571 3797 3949 6058 1493 Size: 2078 226 152 2109 1697 Fragments arranged by size: 2109 2078 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2524 3188 3368 3393 3587 1870 Size: 73 581 664 180 25 194 4545 Fragments arranged by size: 4545 664 581 194 180 73 25 BamHI G'GATC_C Cuts at: 2051 3592 3959 2051 Size: 1541 367 4354 Fragments arranged by size: 4354 1541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3482 3583 5038 618 Size: 325 217 627 1695 101 1455 1842 Fragments arranged by size: 1842 1695 1455 627 325 217 101 BanII G_rGCy'C Cuts at: 1391 2210 2300 2405 3170 3345 3539 3581 1391 Size: 819 90 105 765 175 194 42 4072 Fragments arranged by size: 4072 819 765 194 175 105 90 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3228 928 Size: 971 1329 3962 Fragments arranged by size: 3962 1329 971 BbvCI CC'TCA_GC Cuts at: 2486 2820 2486 Size: 334 5928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 2550 3412 1548 Size: 1002 862 4398 Fragments arranged by size: 4398 1002 862 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 2462 4873 Size: 107 122 71 631 605 766 24 2411 Cuts at: 4873 136 Size: 1525 Fragments arranged by size: 2411 1525 766 631 605 122 107 71 24 BglII A'GATC_T Cuts at: 2907 3084 6257 2907 Size: 177 3173 2912 Fragments arranged by size: 3173 2912 177 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 2310 3180 3539 4323 5479 1790 Size: 520 870 359 784 1156 2573 Fragments arranged by size: 2573 1156 870 784 520 359 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3129 3993 4951 Size: 416 53 83 399 24 1712 864 958 Cuts at: 4951 442 Size: 1753 Fragments arranged by size: 1753 1712 958 864 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2170 3214 2170 Size: 1044 5218 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 2671 3029 3041 3047 3073 4922 1239 Size: 1349 83 358 12 6 26 1849 2579 Fragments arranged by size: 2579 1849 1349 358 83 26 12 6 Bpu10I CC'TnA_GC Cuts at: 2115 2273 2486 2820 2115 Size: 158 213 334 5557 Fragments arranged by size: 5557 334 213 158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4387 5252 5550 4387 Size: 865 298 5099 Fragments arranged by size: 5099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3008 3428 4925 882 Size: 617 1509 420 1497 2219 Fragments arranged by size: 2219 1509 1497 617 420 BsaAI yAC'GTr Cuts at: 493 1204 4053 493 Size: 711 2849 2702 Fragments arranged by size: 2849 2702 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2624 3243 4069 Size: 53 83 186 936 477 614 619 826 Cuts at: 4069 4451 5086 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 826 635 619 614 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2596 2886 4695 5093 5367 5514 5925 2596 Size: 290 1809 398 274 147 411 2933 Fragments arranged by size: 2933 1809 411 398 290 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 3071 3101 1178 Size: 30 165 30 849 30 789 30 4339 Fragments arranged by size: 4339 849 789 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2704 2732 2810 2813 2879 3187 1186 Size: 1518 28 78 3 66 308 4261 Fragments arranged by size: 4261 1518 308 78 66 28 3 BseYI C'CCAG_C Cuts at: 2359 2479 2630 3163 3354 5465 5961 2359 Size: 120 151 533 191 2111 496 2660 Fragments arranged by size: 2660 2111 533 496 191 151 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 1163 Size: 573 894 4795 Fragments arranged by size: 4795 894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2457 3712 4473 5501 1540 Size: 275 186 33 423 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 423 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2121 2262 2378 4323 4408 5479 2121 Size: 141 116 1945 85 1071 2904 Fragments arranged by size: 2904 1945 1071 141 116 85 BsmI GAATG_Cn' Cuts at: 3031 3773 3866 3031 Size: 742 93 5427 Fragments arranged by size: 5427 742 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2210 2262 2300 2310 2378 2405 Size: 399 331 89 52 38 10 68 27 Cuts at: 2405 3170 3180 3345 3539 3581 4323 4408 5479 Size: 765 10 165 194 42 742 85 1071 Cuts at: 5479 1391 Size: 2174 Fragments arranged by size: 2174 1071 765 742 399 331 194 165 89 85 68 52 42 38 27 10 10 BspEI T'CCGG_A Cuts at: 2886 5925 2886 Size: 3039 3223 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3134 3411 3617 844 Size: 2290 277 206 3489 Fragments arranged by size: 3489 2290 277 206 BsrBI CCG'CTC Cuts at: 1394 1823 2277 2673 4149 1394 Size: 429 454 396 1476 3507 Fragments arranged by size: 3507 1476 454 429 396 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3376 4906 5093 1700 Size: 110 1566 1530 187 2869 Fragments arranged by size: 2869 1566 1530 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 2708 1925 Size: 783 5479 BssSI C'ACGA_G Cuts at: 3173 3431 4322 5334 3173 Size: 258 891 1012 4101 Fragments arranged by size: 4101 1012 891 258 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 3073 1184 Size: 1889 4373 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2317 3354 2062 Size: 255 1037 4970 Fragments arranged by size: 4970 1037 255 BstYI r'GATC_y Cuts at: 1972 2051 2074 2907 3084 3529 3592 3959 4344 Size: 79 23 833 177 445 63 367 385 Cuts at: 4344 4361 5813 5899 5911 6257 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 833 445 385 367 346 177 86 79 63 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2415 2442 2451 2460 2638 2703 Size: 552 939 411 27 9 9 178 65 Cuts at: 2703 2718 3336 513 Size: 15 618 3439 Fragments arranged by size: 3439 939 618 552 411 178 65 27 15 9 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 2650 2745 2780 3778 4572 4592 2650 Size: 95 35 998 794 20 4320 Fragments arranged by size: 4320 998 794 95 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2439 2454 Size: 54 1425 50 275 18 201 408 15 Cuts at: 2454 2463 2721 3105 3231 3252 3316 3709 4598 Size: 9 258 384 126 21 64 393 889 Cuts at: 4598 6227 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 408 393 384 275 258 201 126 64 54 50 43 21 18 15 9 EagI C'GGCC_G Cuts at: 1537 1812 2031 2454 3709 1537 Size: 275 219 423 1255 4090 Fragments arranged by size: 4090 1255 423 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2873 4192 1353 Size: 845 675 1319 3423 Fragments arranged by size: 3423 1319 845 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2270 4849 5223 5369 387 Size: 1064 138 681 2579 374 146 1280 Fragments arranged by size: 2579 1280 1064 681 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2588 2671 3029 3041 3047 3073 4325 Size: 393 956 83 358 12 6 26 1252 Cuts at: 4325 4922 5709 5977 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1252 956 787 597 393 358 268 83 26 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2241 2497 2792 3452 2241 Size: 256 295 660 5051 Fragments arranged by size: 5051 660 295 256 EcoO109I rG'GnC_Cy Cuts at: 2521 2602 3036 3123 3536 2521 Size: 81 434 87 413 5247 Fragments arranged by size: 5247 434 413 87 81 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FspI TGC'GCA Cuts at: 2106 4768 2106 Size: 2662 3600 FspAI rTGC'GCAy Cuts at: 2106 2106 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 2291 2360 5409 1678 Size: 613 69 3049 2531 Fragments arranged by size: 3049 2531 613 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4903 4935 4977 5009 1373 Size: 32 135 32 3331 32 42 32 2626 Fragments arranged by size: 3331 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 2645 5376 5560 1305 Size: 762 578 2731 184 2007 Fragments arranged by size: 2731 2007 762 578 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 10 Size: 54 1425 343 633 3807 Fragments arranged by size: 3807 1425 633 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2384 2643 4220 4579 4738 518 Size: 648 1149 69 259 1577 359 159 2042 Fragments arranged by size: 2042 1577 1149 648 359 259 159 69 MspA1I CmG'CkG Cuts at: 1606 2453 2476 2542 3052 3388 3678 4355 5503 Size: 847 23 66 510 336 290 677 1148 Cuts at: 5503 5748 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 847 677 510 336 290 245 66 23 NaeI GCC'GGC Cuts at: 3378 3378 Size: 6262 NcoI C'CATG_G Cuts at: 513 1065 2004 2442 2460 2638 2703 3336 513 Size: 552 939 438 18 178 65 633 3439 Fragments arranged by size: 3439 939 633 552 438 178 65 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NgoMIV G'CCGG_C Cuts at: 3376 3376 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 2454 3709 2454 Size: 1255 5007 NspI r_CATG'y Cuts at: 1132 1229 3620 5165 1132 Size: 97 2391 1545 2229 Fragments arranged by size: 2391 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 3070 3513 1108 Size: 1962 443 3857 Fragments arranged by size: 3857 1962 443 PfoI T'CCnGG_A Cuts at: 2061 2061 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2894 2926 4331 4363 5844 5876 2894 Size: 32 1405 32 1481 32 3280 Fragments arranged by size: 3280 1481 1405 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2602 3036 3123 2602 Size: 434 87 5741 Fragments arranged by size: 5741 434 87 PshAI GACnn'nnGTC Cuts at: 1613 3071 1613 Size: 1458 4804 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 3535 3535 Size: 6262 PstI C_TGCA'G Cuts at: 830 3057 3408 3614 830 Size: 2227 351 206 3478 Fragments arranged by size: 3478 2227 351 206 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 2542 3052 2542 Size: 510 5752 SacII CC_GC'GG Cuts at: 2454 2454 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SapI GCTCTTCn'nnn_ Cuts at: 2198 2198 Size: 6262 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SexAI A'CCwGG_T Cuts at: 2604 2604 Size: 6262 SfcI C'TryA_G Cuts at: 826 1045 3053 3404 3610 4745 5426 5617 826 Size: 219 2008 351 206 1135 681 191 1471 Fragments arranged by size: 2008 1471 1135 681 351 219 206 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2462 2462 Size: 6262 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 2526 3370 3395 3589 1872 Size: 654 844 25 194 4545 Fragments arranged by size: 4545 844 654 194 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 4366 5267 5529 820 Size: 197 926 2423 901 262 1553 Fragments arranged by size: 2423 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 2873 3458 1517 Size: 1356 585 4321 Fragments arranged by size: 4321 1356 585 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 2460 2638 2703 3336 3507 Size: 552 939 438 18 178 65 633 171 Cuts at: 3507 3701 513 Size: 194 3074 Fragments arranged by size: 3074 939 633 552 438 194 178 171 65 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2345 3128 3443 4289 4306 4459 4644 Size: 1255 765 783 315 846 17 153 185 Cuts at: 4644 5089 325 Size: 445 1498 Fragments arranged by size: 1498 1255 846 783 765 445 315 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4508 6220 96 Size: 274 80 33 51 1443 2531 1712 138 Fragments arranged by size: 2531 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 6222 6254 2252 Size: 32 3938 32 2260 Fragments arranged by size: 3938 2260 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2012 3242 1316 Size: 696 1230 4336 Fragments arranged by size: 4336 1230 696 XbaI T'CTAG_A Cuts at: 2956 3598 2956 Size: 642 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 2524 3368 3393 3587 1870 Size: 654 844 25 194 4545 Fragments arranged by size: 4545 844 654 194 25 XmnI GAAnn'nnTTC Cuts at: 2579 4391 2579 Size: 1812 4450 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfoI SrfI SwaI