Restriction map for
pFN21AB7794
(Linear) (Six-base) MAPSORT of: KIBB7794.seq Check: 1187 from: 1 to: 1557
KIBB7794.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1406 1557
Size: 1406 151
AccI GT'mk_AC
Cuts at: 0 1241 1557
Size: 1241 316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1158 1339 1342 1557
Size: 1158 181 3 215
Fragments arranged by size:
1158 215 181 3
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1065 1557
Size: 1047 18 492
Fragments arranged by size:
1047 492 18
ApaI G_GGCC'C
Cuts at: 0 1534 1557
Size: 1534 23
AvaI C'yCGr_G
Cuts at: 0 519 1183 1363 1388 1557
Size: 519 664 180 25 169
Fragments arranged by size:
664 519 180 169 25
BamHI G'GATC_C
Cuts at: 0 46 1557
Size: 46 1511
BanI G'GyrC_C
Cuts at: 0 1477 1557
Size: 1477 80
BanII G_rGCy'C
Cuts at: 0 205 295 400 1165 1340 1534 1557
Size: 205 90 105 765 175 194 23
Fragments arranged by size:
765 205 194 175 105 90 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 1223 1557
Size: 1223 334
BbvCI CC'TCA_GC
Cuts at: 0 481 815 1557
Size: 481 334 742
Fragments arranged by size:
742 481 334
BclI T'GATC_A
Cuts at: 0 545 1407 1557
Size: 545 862 150
Fragments arranged by size:
862 545 150
BglI GCCn_nnn'nGGC
Cuts at: 0 433 457 1557
Size: 433 24 1100
Fragments arranged by size:
1100 433 24
BglII A'GATC_T
Cuts at: 0 902 1079 1557
Size: 902 177 478
Fragments arranged by size:
902 478 177
Bme1580I G_kGCm'C
Cuts at: 0 305 1175 1534 1557
Size: 305 870 359 23
Fragments arranged by size:
870 359 305 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 1124 1557
Size: 1124 433
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 165 1209 1557
Size: 165 1044 348
Fragments arranged by size:
1044 348 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 666 1024 1036 1042 1068 1557
Size: 583 83 358 12 6 26 489
Fragments arranged by size:
583 489 358 83 26 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 110 268 481 815 1557
Size: 110 158 213 334 742
Fragments arranged by size:
742 334 213 158 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 1003 1423 1557
Size: 1003 420 134
Fragments arranged by size:
1003 420 134
BsaHI Gr'CG_yC
Cuts at: 0 5 619 1238 1557
Size: 5 614 619 319
Fragments arranged by size:
619 614 319 5
BsaWI w'CCGG_w
Cuts at: 0 591 881 1557
Size: 591 290 676
Fragments arranged by size:
676 591 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 1066 1096 1557
Size: 247 30 789 30 461
Fragments arranged by size:
789 461 247 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 699 727 805 808 874 1182 1557
Size: 699 28 78 3 66 308 375
Fragments arranged by size:
699 375 308 78 66 28 3
BseYI C'CCAG_C
Cuts at: 0 354 474 625 1158 1349 1557
Size: 354 120 151 533 191 208
Fragments arranged by size:
533 354 208 191 151 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1557
Size: 625 932
BsiEI CG_ry'CG
Cuts at: 0 29 452 1557
Size: 29 423 1105
Fragments arranged by size:
1105 423 29
BsiHKAI G_wGCw'C
Cuts at: 0 116 257 373 1557
Size: 116 141 116 1184
Fragments arranged by size:
1184 141 116 116
BsmI GAATG_Cn'
Cuts at: 0 1026 1557
Size: 1026 531
Bsp1286I G_dGCh'C
Cuts at: 0 116 205 257 295 305 373 400 1165
Size: 116 89 52 38 10 68 27 765
Cuts at: 1165 1175 1340 1534 1557
Size: 10 165 194 23
Fragments arranged by size:
765 194 165 116 89 68 52 38
27 23 10 10
BspEI T'CCGG_A
Cuts at: 0 881 1557
Size: 881 676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1129 1406 1557
Size: 1129 277 151
Fragments arranged by size:
1129 277 151
BsrBI CCG'CTC
Cuts at: 0 272 668 1557
Size: 272 396 889
Fragments arranged by size:
889 396 272
BsrFI r'CCGG_y
Cuts at: 0 1371 1557
Size: 1371 186
BssHII G'CGCG_C
Cuts at: 0 703 1557
Size: 703 854
BssSI C'ACGA_G
Cuts at: 0 1168 1426 1557
Size: 1168 258 131
Fragments arranged by size:
1168 258 131
BstEII G'GTnAC_C
Cuts at: 0 1068 1557
Size: 1068 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 312 1349 1557
Size: 57 255 1037 208
Fragments arranged by size:
1037 255 208 57
BstYI r'GATC_y
Cuts at: 0 46 69 902 1079 1524 1557
Size: 46 23 833 177 445 33
Fragments arranged by size:
833 445 177 46 33 23
BtgI C'CryG_G
Cuts at: 0 410 437 446 455 633 698 713 1331
Size: 410 27 9 9 178 65 15 618
Cuts at: 1331 1557
Size: 226
Fragments arranged by size:
618 410 226 178 65 27 15 9
9
BtsI GCAGTG_nn'
Cuts at: 0 645 740 775 1557
Size: 645 95 35 782
Fragments arranged by size:
782 645 95 35
EaeI y'GGCC_r
Cuts at: 0 26 434 449 458 716 1100 1226 1247
Size: 26 408 15 9 258 384 126 21
Cuts at: 1247 1311 1557
Size: 64 246
Fragments arranged by size:
408 384 258 246 126 64 26 21
15 9
EagI C'GGCC_G
Cuts at: 0 26 449 1557
Size: 26 423 1108
Fragments arranged by size:
1108 423 26
EarI CTCTTCn'nnn_
Cuts at: 0 193 868 1557
Size: 193 675 689
Fragments arranged by size:
689 675 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 1557
Size: 265 1292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 666 1024 1036 1042 1068 1557
Size: 583 83 358 12 6 26 489
Fragments arranged by size:
583 489 358 83 26 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 236 492 787 1447 1557
Size: 236 256 295 660 110
Fragments arranged by size:
660 295 256 236 110
EcoO109I rG'GnC_Cy
Cuts at: 0 516 597 1031 1118 1531 1557
Size: 516 81 434 87 413 26
Fragments arranged by size:
516 434 413 87 81 26
FspI TGC'GCA
Cuts at: 0 101 1557
Size: 101 1456
FspAI rTGC'GCAy
Cuts at: 0 101 1557
Size: 101 1456
HaeII r_GCGC'y
Cuts at: 0 286 355 1557
Size: 286 69 1202
Fragments arranged by size:
1202 286 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 640 1557
Size: 62 578 917
Fragments arranged by size:
917 578 62
MscI TGG'CCA
Cuts at: 0 460 1557
Size: 460 1097
MslI CAynn'nnrTG
Cuts at: 0 310 379 638 1557
Size: 310 69 259 919
Fragments arranged by size:
919 310 259 69
MspA1I CmG'CkG
Cuts at: 0 448 471 537 1047 1383 1557
Size: 448 23 66 510 336 174
Fragments arranged by size:
510 448 336 174 66 23
NaeI GCC'GGC
Cuts at: 0 1373 1557
Size: 1373 184
NcoI C'CATG_G
Cuts at: 0 437 455 633 698 1331 1557
Size: 437 18 178 65 633 226
Fragments arranged by size:
633 437 226 178 65 18
NgoMIV G'CCGG_C
Cuts at: 0 1371 1557
Size: 1371 186
NotI GC'GGCC_GC
Cuts at: 0 449 1557
Size: 449 1108
PflMI CCAn_nnn'nTGG
Cuts at: 0 1065 1508 1557
Size: 1065 443 49
Fragments arranged by size:
1065 443 49
PfoI T'CCnGG_A
Cuts at: 0 56 1557
Size: 56 1501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 889 921 1557
Size: 889 32 636
Fragments arranged by size:
889 636 32
PpuMI rG'GwC_Cy
Cuts at: 0 597 1031 1118 1557
Size: 597 434 87 439
Fragments arranged by size:
597 439 434 87
PshAI GACnn'nnGTC
Cuts at: 0 1066 1557
Size: 1066 491
PspOMI G'GGCC_C
Cuts at: 0 1530 1557
Size: 1530 27
PstI C_TGCA'G
Cuts at: 0 1052 1403 1557
Size: 1052 351 154
Fragments arranged by size:
1052 351 154
PvuII CAG'CTG
Cuts at: 0 537 1047 1557
Size: 537 510 510
Fragments arranged by size:
537 510 510
SacII CC_GC'GG
Cuts at: 0 449 1557
Size: 449 1108
SapI GCTCTTCn'nnn_
Cuts at: 0 193 1557
Size: 193 1364
SexAI A'CCwGG_T
Cuts at: 0 599 1557
Size: 599 958
SfcI C'TryA_G
Cuts at: 0 1048 1399 1557
Size: 1048 351 158
Fragments arranged by size:
1048 351 158
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 457 1557
Size: 457 1100
SmaI CCC'GGG
Cuts at: 0 521 1365 1390 1557
Size: 521 844 25 167
Fragments arranged by size:
844 521 167 25
StuI AGG'CCT
Cuts at: 0 868 1453 1557
Size: 868 585 104
Fragments arranged by size:
868 585 104
StyI C'CwwG_G
Cuts at: 0 437 455 633 698 1331 1502 1557
Size: 437 18 178 65 633 171 55
Fragments arranged by size:
633 437 178 171 65 55 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 1123 1438 1557
Size: 340 783 315 119
Fragments arranged by size:
783 340 315 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 1557
Size: 247 32 1278
Fragments arranged by size:
1278 247 32
Tth111I GACn'n_nGTC
Cuts at: 0 7 1237 1557
Size: 7 1230 320
Fragments arranged by size:
1230 320 7
XbaI T'CTAG_A
Cuts at: 0 951 1557
Size: 951 606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1557
Size: 471 1086
XmaI C'CCGG_G
Cuts at: 0 519 1363 1388 1557
Size: 519 844 25 169
Fragments arranged by size:
844 519 169 25
XmnI GAAnn'nnTTC
Cuts at: 0 574 1557
Size: 574 983
Enzymes that do cut:
AarI AccI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BclI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrFI BssHII BssSI BstEII BstXI BstYI BtgI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NotI PflMI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SacII SapI SexAI SfcI SfiI
SmaI StuI StyI TaqII TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BfrBI BlpI BmgBI
BmtI BpuEI BsaAI BsaBI BsiWI BsmBI BspHI BsrDI
BsrGI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
XhoI ZraI