Restriction map for
pFN21AB6708
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HapII Hin1I HincII HindII
HindIII HinfI HpaI HpaII HphI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 190 GTAC GT^AC
AocI 48 CCTGAGG CC^T[ACGT]AGG
AvaII 177 GGACC G^G[AT]CC
BbsI 223 GAAGAC GAAGAC(2/6)
BpmI 98 CTGGAG CTGGAG(16/14)
BpuAI 223 GAAGAC GAAGAC(2/6)
BsgI 313 CTGCAC GTGCAG(16/14)
BsiEI 74 CGGCCG CG[GA][CT]^CG
BssKI 218 CCAGG ^CC[ACGT]GG
BstNI 218 CCAGG CC^[AT]GG
Bsu36I 48 CCTGAGG CC^T[ACGT]AGG
DdeI 49 CTGAG C^T[ACGT]AG
DpnII 150 GATC ^GATC
DraII 44 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 89 CCATGG C^C[GA][CT]GG
EagI 74 CGGCCG C^GGCCG
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
Eco81I 48 CCTGAGG CC^T[ACGT]AGG
EcoO109I 44 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 218 CCAGG ^CC[AT]GG
EcoT14I 89 CCATGG C^C[AT][AT]GG
FauI 341 CCCGC CCCGC(4/6)
HgaI 296 GCGTC GACGC(5/10)
MaeIII 12 GTTAC ^GT[ACGT]AC
MboI 150 GATC ^GATC
MvaI 218 CCAGG CC^[AT]GG
NcoI 89 CCATGG C^CATGG
NdeII 150 GATC ^GATC
NotI 73 GCGGCCGC GC^GGCCGC
PflMI 372 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 376 CAGCTG CAG^CTG
RsaI 190 GTAC GT^AC
SapI 121 GAAGAGC GCTCTTC(1/4)
Sau3AI 150 GATC ^GATC
ScrFI 218 CCAGG CC^[ACGT]GG
SfiI 36 GGCCGCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 89 CCATGG C^C[AT][AT]GG
Tsp509I 232 AATT ^AATT
Van91I 372 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 43 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
373 CAGCAGCTG
BanI 214 GGCACC G^G[CT][GA]CC
366 GGTGCC
BanII 53 GGGCTC G[GA]GC[CT]^C
350 GAGCCC
BsaJI 89 CCATGG C^C[ACGT][ACGT]GG
218 CCAGGG
BsiYI 340 CCCCGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCAGCAGCTGG
BslI 340 CCCCGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCAGCAGCTGG
BsmFI 171 GGGAC GGGAC(10/14)
238 GTCCC
EaeI 35 TGGCCG [CT]^GGCC[GA]
74 CGGCCG
EarI 121 GAAGAG CTCTTC(1/4)
280 GAAGAG
Ksp632I 121 GAAGAG CTCTTC(1/4)
280 GAAGAG
MspA1I 342 CCGCTG C[AC]G^C[GT]G
376 CAGCTG
NlaIII 90 CATG CATG^
153 CATG
TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^
114 CACTG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI BbvI BglI
BmyI Bsp1286I BsrI BstUI Cac8I
CfoI Cfr13I Fnu4HI HaeIII HhaI
HinP1I ItaI MboII MnlI MvnI
MwoI NlaIV Sau96I SfaNI TseI