Restriction map for
pFN21AB7835
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AspEI
AspI AvaI AviII BalI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BglII BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HindIII
HpaII KasI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 345 GGTACC G^GTACC
AflIII 122 ACATGT A^C[GA][CT]GT
Alw44I 239 GTGCAC G^TGCAC
ApaLI 239 GTGCAC G^TGCAC
AseI 741 ATTAAT AT^TAAT
AsnI 741 ATTAAT AT^TAAT
Asp718I 345 GGTACC G^GTACC
AvrII 406 CCTAGG C^CTAGG
BamHI 1001 GGATCC G^GATCC
BanII 764 GAGCTC G[GA]GC[CT]^C
BcgI 537 GCAGCCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 406 CCTAGG C^CTAGG
BlpI 1606 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1606 GCTCAGC GC^T[ACGT]AGC
BseRI 1355 GAGGAG GAGGAG(10/8)
BsmBI 1358 GAGACG CGTCTC(1/5)
BspLU11I 122 ACATGT A^CATGT
BspMI 1577 ACCTGC ACCTGC(4/8)
BstEII 727 GGTAACC G^GT[ACGT]ACC
BstPI 727 GGTAACC G^GT[ACGT]ACC
CelII 1606 GCTCAGC GC^T[ACGT]AGC
EaeI 1308 TGGCCG [CT]^GGCC[GA]
EcoNI 1478 CCTATGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 727 GGTAACC G^GT[ACGT]ACC
EcoRI 449 GAATTC G^AATTC
HgaI 1198 GCGTC GACGC(5/10)
HpaI 1080 GTTAAC GTT^AAC
KpnI 345 GGTACC GGTAC^C
MspA1I 199 CAGCTG C[AC]G^C[GT]G
PshBI 741 ATTAAT AT^TAAT
PstI 1379 CTGCAG CTGCA^G
PvuII 199 CAGCTG CAG^CTG
SacI 764 GAGCTC GAGCT^C
SapI 761 GAAGAGC GCTCTTC(1/4)
ScaI 1259 AGTACT AGT^ACT
SexAI 44 ACCAGGT A^CC[AT]GGT
XcmI 423 CCACTCCCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 97 GTCGAC GT^[AC][GT]AC
1223 GTCGAC
Asp700I 832 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1321 GAAGACCTTC
AspHI 239 GTGCAC G[AT]GC[AT]^C
764 GAGCTC
BanI 345 GGTACC G^G[CT][GA]CC
1443 GGCACC
BbsI 1321 GAAGAC GAAGAC(2/6)
138 GTCTTC
BpuAI 1321 GAAGAC GAAGAC(2/6)
138 GTCTTC
BsiHKAI 239 GTGCAC G[AT]GC[AT]^C
764 GAGCTC
BsrI 1517 ACTGG ACTGG(1/-1)
135 CCAGT
BstYI 867 AGATCC [GA]^GATC[CT]
1001 GGATCC
DpnII 868 GATC ^GATC
1002 GATC
EarI 1330 CTCTTC CTCTTC(1/4)
761 GAAGAG
EcoT14I 406 CCTAGG C^C[AT][AT]GG
752 CCTTGG
HinfI 402 GAATC G^A[ACGT]TC
594 GAATC
Ksp632I 1330 CTCTTC CTCTTC(1/4)
761 GAAGAG
MaeII 326 ACGT A^CGT
1361 ACGT
MboI 868 GATC ^GATC
1002 GATC
MflI 867 AGATCC [GA]^GATC[CT]
1001 GGATCC
NdeII 868 GATC ^GATC
1002 GATC
NspI 59 GCATGT [GA]CATG^[CT]
122 ACATGT
SalI 97 GTCGAC G^TCGAC
1223 GTCGAC
Sau3AI 868 GATC ^GATC
1002 GATC
SfaNI 178 GCATC GCATC(5/9)
1210 GCATC
SfcI 129 CTACAG C^T[GA][CT]AG
1379 CTGCAG
SmlI 1040 CTCAAG C^T[CT][GA]AG
1229 CTCAAG
StuI 246 AGGCCT AGG^CCT
1155 AGGCCT
StyI 406 CCTAGG C^C[AT][AT]GG
752 CCTTGG
TfiI 402 GAATC G^A[AT]TC
594 GAATC
TspRI 102 CAGTG CA[GC]TG[ACGT][ACGT]^
1382 CAGTG
XhoII 867 AGATCC [GA]^GATC[CT]
1001 GGATCC
XmnI 832 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1321 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HincII HindII HphI ItaI MaeI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI TaqI Tru9I TseI Tsp45I
Tsp509I TthHB8I