Restriction map for pFN21AA0034
 (Linear) (Six-base) MAPSORT of: KIAA0034.seq  Check: 1656  from: 1  to: 5025



KIAA0034.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:24  ..

AccI GT'mk_AC

Cuts at:      0    2430    5025 
   Size:       2430    2595
 
Acc65I G'GTAC_C

Cuts at:      0     868    5025 
   Size:        868    4157
 
AclI AA'CG_TT

Cuts at:      0     640    3300    5025 
   Size:        640    2660    1725

  Fragments arranged by size: 

                2660    1725     640
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     247    1100    1228    1303    1400    1729    1748 
   Size:         63     184     853     128      75      97     329      19

Cuts at:   1748    2495    2975    3221    4670    4829    5025 
   Size:        747     480     246    1449     159     196

  Fragments arranged by size: 

                1449     853     747     480     329     246     196     184
                 159     128      97      75      63      19
 
AflII C'TTAA_G

Cuts at:      0    2287    5025 
   Size:       2287    2738
 
AflIII A'CryG_T

Cuts at:      0    3112    3114    5025 
   Size:       3112       2    1911

  Fragments arranged by size: 

                3112    1911       2
 
AleI CACnn'nnGTG

Cuts at:      0    3701    4298    5025 
   Size:       3701     597     727

  Fragments arranged by size: 

                3701     727     597
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1703    2893    5025 
   Size:       1703    1190    2132

  Fragments arranged by size: 

                2132    1703    1190
 
AlwNI CAG_nnn'CTG

Cuts at:      0      12      51    2034    2091    2971    4437    5025 
   Size:         12      39    1983      57     880    1466     588

  Fragments arranged by size: 

                1983    1466     880     588      57      39      12
 
ApaLI G'TGCA_C

Cuts at:      0    2179    5025 
   Size:       2179    2846
 
ApoI r'AATT_y

Cuts at:      0     106    1099    1169    2280    2594    2808    3220    3562 
   Size:        106     993      70    1111     314     214     412     342

Cuts at:   3562    3793    3976    4237    5025 
   Size:        231     183     261     788

  Fragments arranged by size: 

                1111     993     788     412     342     314     261     231
                 214     183     106      70
 
AseI AT'TA_AT

Cuts at:      0    2792    5025 
   Size:       2792    2233
 
AvaI C'yCGr_G

Cuts at:      0    3491    5025 
   Size:       3491    1534
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      39      72    1239    1272    5025 
   Size:         39      33    1167      33    3753

  Fragments arranged by size: 

                3753    1167      39      33      33
 
BamHI G'GATC_C

Cuts at:      0    2146    2853    5025 
   Size:       2146     707    2172

  Fragments arranged by size: 

                2172    2146     707
 
BanI G'GyrC_C

Cuts at:      0     449     868    4956    5025 
   Size:        449     419    4088      69

  Fragments arranged by size: 

                4088     449     419      69
 
BanII G_rGCy'C

Cuts at:      0    1833    3936    5025 
   Size:       1833    2103    1089

  Fragments arranged by size: 

                2103    1833    1089
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     195     741    1988    5025 
   Size:        195     546    1247    3037

  Fragments arranged by size: 

                3037    1247     546     195
 
BbvCI CC'TCA_GC

Cuts at:      0    2540    5025 
   Size:       2540    2485
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1267    1519    5025 
   Size:       1267     252    3506

  Fragments arranged by size: 

                3506    1267     252
 
BclI T'GATC_A

Cuts at:      0    3896    5025 
   Size:       3896    1129
 
BfrBI ATG'CAT

Cuts at:      0    1770    5025 
   Size:       1770    3255
 
BlpI GC'TnA_GC

Cuts at:      0    4487    5025 
   Size:       4487     538
 
Bme1580I G_kGCm'C

Cuts at:      0    2183    5025 
   Size:       2183    2842
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1396    2222    3336    3456    3928    4905    4928    5025 
   Size:       1396     826    1114     120     472     977      23      97

  Fragments arranged by size: 

                1396    1114     977     826     472     120      97      23
            
 
BmtI G_CTAG'C

Cuts at:      0     230     599    5025 
   Size:        230     369    4426

  Fragments arranged by size: 

                4426     369     230
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      28     408     692     743    1166    1517    3043 
   Size:         22       6     380     284      51     423     351    1526

Cuts at:   3043    3342    4027    5025 
   Size:        299     685     998

  Fragments arranged by size: 

                1526     998     685     423     380     351     299     284
                  51      22       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    2267    2540    2634    5025 
   Size:       2267     273      94    2391

  Fragments arranged by size: 

                2391    2267     273      94
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     880    1763    1774    3946    5025 
   Size:        880     883      11    2172    1079

  Fragments arranged by size: 

                2172    1079     883     880      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2134    2895    5025 
   Size:       2134     761    2130

  Fragments arranged by size: 

                2134    2130     761
 
BsaAI yAC'GTr

Cuts at:      0    1060    3115    5025 
   Size:       1060    2055    1910

  Fragments arranged by size: 

                2055    1910    1060
 
BsaBI GATnn'nnATC

Cuts at:      0    2741    5025 
   Size:       2741    2284
 
BsaWI w'CCGG_w

Cuts at:      0    3215    5025 
   Size:       3215    1810
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1336    1366    5025 
   Size:       1336      30    3659

  Fragments arranged by size: 

                3659    1336      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1226    2626    5025 
   Size:       1226    1400    2399

  Fragments arranged by size: 

                2399    1400    1226
 
BseYI C'CCAG_C

Cuts at:      0      63     250    1212    1217    5025 
   Size:         63     187     962       5    3808

  Fragments arranged by size: 

                3808     962     187      63       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     902    1934    4322    5025 
   Size:        497     405    1032    2388     703

  Fragments arranged by size: 

                2388    1032     703     497     405
 
BsiHKAI G_wGCw'C

Cuts at:      0    2183    2366    3936    4004    5025 
   Size:       2183     183    1570      68    1021

  Fragments arranged by size: 

                2183    1570    1021     183      68
 
BsmI GAATG_Cn'

Cuts at:      0    1690    5025 
   Size:       1690    3335
 
Bsp1286I G_dGCh'C

Cuts at:      0    1833    2183    2366    3936    4004    5025 
   Size:       1833     350     183    1570      68    1021

  Fragments arranged by size: 

                1833    1570    1021     350     183      68
 
BspEI T'CCGG_A

Cuts at:      0    3215    5025 
   Size:       3215    1810
 
BspHI T'CATG_A

Cuts at:      0     215     924    2067    2598    2984    4805    5025 
   Size:        215     709    1143     531     386    1821     220

  Fragments arranged by size: 

                1821    1143     709     531     386     220     215
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     880    3321    5025 
   Size:        880    2441    1704

  Fragments arranged by size: 

                2441    1704     880
 
BsrDI GCAATG_nn'

Cuts at:      0     794    2808    3189    3312    4616    5025 
   Size:        794    2014     381     123    1304     409

  Fragments arranged by size: 

                2014    1304     794     409     381     123
 
BsrFI r'CCGG_y

Cuts at:      0    1072    5025 
   Size:       1072    3953
 
BsrGI T'GTAC_A

Cuts at:      0    2409    2922    3650    3831    5025 
   Size:       2409     513     728     181    1194

  Fragments arranged by size: 

                2409    1194     728     513     181
 
BssSI C'ACGA_G

Cuts at:      0    4298    5025 
   Size:       4298     727
 
BstBI TT'CG_AA

Cuts at:      0     186    5025 
   Size:        186    4839
 
BstEII G'GTnAC_C

Cuts at:      0    3604    5025 
   Size:       3604    1421
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2864    3601    5025 
   Size:       2864     737    1424

  Fragments arranged by size: 

                2864    1424     737
 
BstYI r'GATC_y

Cuts at:      0     338    2146    2736    2853    5025 
   Size:        338    1808     590     117    2172

  Fragments arranged by size: 

                2172    1808     590     338     117
 
Bsu36I CC'TnA_GG

Cuts at:      0    1438    5025 
   Size:       1438    3587
 
BtgI C'CryG_G

Cuts at:      0    4282    5025 
   Size:       4282     743
 
BtsI GCAGTG_nn'

Cuts at:      0     746    1687    1922    3089    4669    5025 
   Size:        746     941     235    1167    1580     356

  Fragments arranged by size: 

                1580    1167     941     746     356     235
 
ClaI AT'CG_AT

Cuts at:      0    2337    5025 
   Size:       2337    2688
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3420    3455    5025 
   Size:       3420      35    1570

  Fragments arranged by size: 

                3420    1570      35
 
DraI TTT'AAA

Cuts at:      0    2187    5025 
   Size:       2187    2838
 
DraIII CAC_nnn'GTG

Cuts at:      0    1429    5025 
   Size:       1429    3596
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      96    5025 
   Size:         96    4929
 
EaeI y'GGCC_r

Cuts at:      0    2769    5025 
   Size:       2769    2256
 
EarI CTCTTCn'nnn_

Cuts at:      0     266    1613    2725    2821    3884    4546    5025 
   Size:        266    1347    1112      96    1063     662     479

  Fragments arranged by size: 

                1347    1112    1063     662     479     266      96
 
EcoICRI GAG'CTC

Cuts at:      0    3934    5025 
   Size:       3934    1091
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      28      63     247     408     692     743    1100 
   Size:         22       6      35     184     161     284      51     357

Cuts at:   1100    1166    1228    1303    1400    1517    1729    1748    2495 
   Size:         66      62      75      97     117     212      19     747

Cuts at:   2495    2975    3043    3221    3342    4027    4670    4829    5025 
   Size:        480      68     178     121     685     643     159     196

  Fragments arranged by size: 

                 747     685     643     480     357     284     212     196
                 184     178     161     159     121     117      97      75
                  68      66      62      51      35      22      19       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4600    5025 
   Size:       4600     425
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1732    2169    2949    4930    5025 
   Size:       1732     437     780    1981      95

  Fragments arranged by size: 

                1981    1732     780     437      95
 
EcoRI G'AATT_C

Cuts at:      0    1169    2594    2808    3793    4237    5025 
   Size:       1169    1425     214     985     444     788

  Fragments arranged by size: 

                1425    1169     985     788     444     214
 
EcoRV GAT'ATC

Cuts at:      0    4179    5025 
   Size:       4179     846
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1642    2909    4084    4612    4833    5025 
   Size:       1642    1267    1175     528     221     192

  Fragments arranged by size: 

                1642    1267    1175     528     221     192
 
HaeII r_GCGC'y

Cuts at:      0     212    5025 
   Size:        212    4813
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    3388    3420    4125    4157    4462    4494    5025 
   Size:       3388      32     705      32     305      32     531

  Fragments arranged by size: 

                3388     705     531     305      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1956    2081    2157    2431    2466    3328    5025 
   Size:       1956     125      76     274      35     862    1697

  Fragments arranged by size: 

                1956    1697     862     274     125      76      35
 
HpaI GTT'AAC

Cuts at:      0    2157    5025 
   Size:       2157    2868
 
KpnI G_GTAC'C

Cuts at:      0     872    5025 
   Size:        872    4153
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1503    2694    3618    3887    4168    4268    5025 
   Size:       1503    1191     924     269     281     100     757

  Fragments arranged by size: 

                1503    1191     924     757     281     269     100
 
MscI TGG'CCA

Cuts at:      0    2771    5025 
   Size:       2771    2254
 
MslI CAynn'nnrTG

Cuts at:      0     160     280     851     880    3701    3839    3943    4298 
   Size:        160     120     571      29    2821     138     104     355

Cuts at:   4298    5025 
   Size:        727

  Fragments arranged by size: 

                2821     727     571     355     160     138     120     104
                  29
 
MspA1I CmG'CkG

Cuts at:      0     937    3718    5025 
   Size:        937    2781    1307

  Fragments arranged by size: 

                2781    1307     937
 
NdeI CA'TA_TG

Cuts at:      0    3285    5025 
   Size:       3285    1740
 
NheI G'CTAG_C

Cuts at:      0     226     595    5025 
   Size:        226     369    4430

  Fragments arranged by size: 

                4430     369     226
 
NsiI A_TGCA'T

Cuts at:      0    1772    5025 
   Size:       1772    3253
 
NspI r_CATG'y

Cuts at:      0     592    1774    3838    5025 
   Size:        592    1182    2064    1187

  Fragments arranged by size: 

                2064    1187    1182     592
 
PasI CC'CwG_GG

Cuts at:      0    1119    5025 
   Size:       1119    3906
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      51    2358    4990    5005    5025 
   Size:         51    2307    2632      15      20

  Fragments arranged by size: 

                2632    2307      51      20      15
 
PmlI CAC'GTG

Cuts at:      0    3115    5025 
   Size:       3115    1910
 
PpuMI rG'GwC_Cy

Cuts at:      0    1732    2169    2949    4930    5025 
   Size:       1732     437     780    1981      95

  Fragments arranged by size: 

                1981    1732     780     437      95
 
PstI C_TGCA'G

Cuts at:      0    1871    2039    2994    4056    4664    5025 
   Size:       1871     168     955    1062     608     361

  Fragments arranged by size: 

                1871    1062     955     608     361     168
 
PvuII CAG'CTG

Cuts at:      0     937    3718    5025 
   Size:        937    2781    1307

  Fragments arranged by size: 

                2781    1307     937
 
SacI G_AGCT'C

Cuts at:      0    3936    5025 
   Size:       3936    1089
 
SalI G'TCGA_C

Cuts at:      0    2429    5025 
   Size:       2429    2596
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1613    3884    5025 
   Size:       1613    2271    1141

  Fragments arranged by size: 

                2271    1613    1141
 
ScaI AGT'ACT

Cuts at:      0    1249    3466    3747    4816    5025 
   Size:       1249    2217     281    1069     209

  Fragments arranged by size: 

                2217    1249    1069     281     209
 
SexAI A'CCwGG_T

Cuts at:      0    2837    2915    5025 
   Size:       2837      78    2110

  Fragments arranged by size: 

                2837    2110      78
 
SfcI C'TryA_G

Cuts at:      0     554     710    1867    2035    2241    2438    2990    4052 
   Size:        554     156    1157     168     206     197     552    1062

Cuts at:   4052    4212    4660    4877    5025 
   Size:        160     448     217     148

  Fragments arranged by size: 

                1157    1062     554     552     448     217     206     197
                 168     160     156     148
 
SmlI C'TyrA_G

Cuts at:      0     859    1753    1778    2287    3491    3925    5025 
   Size:        859     894      25     509    1204     434    1100

  Fragments arranged by size: 

                1204    1100     894     859     509     434      25
 
SnaBI TAC'GTA

Cuts at:      0    1060    5025 
   Size:       1060    3965
 
SpeI A'CTAG_T

Cuts at:      0    3436    5025 
   Size:       3436    1589
 
SphI G_CATG'C

Cuts at:      0    1774    5025 
   Size:       1774    3251
 
SspI AAT'ATT

Cuts at:      0    4032    5025 
   Size:       4032     993
 
StuI AGG'CCT

Cuts at:      0    1311    5025 
   Size:       1311    3714
 
StyI C'CwwG_G

Cuts at:      0    2578    4037    4187    5025 
   Size:       2578    1459     150     838

  Fragments arranged by size: 

                2578    1459     838     150
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1378    1942    3691    5025 
   Size:       1378     564    1749    1334

  Fragments arranged by size: 

                1749    1378    1334     564
 
TatI w'GTAC_w

Cuts at:      0    1247    1314    1684    2409    2922    3464    3650    3745 
   Size:       1247      67     370     725     513     542     186      95

Cuts at:   3745    3831    4814    5025 
   Size:         86     983     211

  Fragments arranged by size: 

                1247     983     725     542     513     370     211     186
                  95      86      67
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4554    4586    5025 
   Size:       4554      32     439

  Fragments arranged by size: 

                4554     439      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2347    5025 
   Size:       2347    2678
 
XbaI T'CTAG_A

Cuts at:      0    4021    5025 
   Size:       4021    1004
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4151    4960    5025 
   Size:       4151     809      65

  Fragments arranged by size: 

                4151     809      65
 
XhoI C'TCGA_G

Cuts at:      0    3491    5025 
   Size:       3491    1534
 
XmnI GAAnn'nnTTC

Cuts at:      0    3419    3793    5025 
   Size:       3419     374    1232

  Fragments arranged by size: 

                3419    1232     374
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI     AvaI     BaeI    BamHI     BanI
    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BlpI Bme1580I
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII     HpaI
     KpnI     MmeI     MscI     MslI   MspA1I     NdeI     NheI     NsiI
     NspI     PasI    PflMI     PmlI    PpuMI     PstI    PvuII     SacI
     SalI     SapI     ScaI    SexAI     SfcI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AloI     ApaI     AscI
    AsiSI    AvrII     BbeI     BcgI     BglI    BglII    BmgBI     BplI
    BsaHI    BsiEI    BsiWI    BsmBI    BsrBI   BssHII   BstAPI  BstZ17I
    BtgZI     EagI     EciI     FseI     FspI    FspAI  HindIII     KasI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     PacI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SrfI     SwaI     XmaI     ZraI
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