Restriction map for pFN21AE0494
 (Circular) (Six-base) MAPSORT of: pFN21AE0494.seq  Check: 9473  from: 1  to: 5359



pFN21AE0494  5359 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2086    2330    2086 
   Size:        244    5115
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3169     278 
   Size:         53      83     186     936    1633    2468

  Fragments arranged by size: 

                2468    1633     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2702    1937 
   Size:        765    4594
 
Acc65I G'GTAC_C

Cuts at:   2680    2680 
   Size:       5359
 
AclI AA'CG_TT

Cuts at:   1563    3486    3859    1563 
   Size:       1923     373    3063

  Fragments arranged by size: 

                3063    1923     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3422    4806    5074    1632 
   Size:       1790    1384     268    1917

  Fragments arranged by size: 

                1917    1790    1384     268
 
AfeI AGC'GCT

Cuts at:   2493    2493 
   Size:       5359
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5162
 
AflIII A'CryG_T

Cuts at:   1203    4258    5235    1203 
   Size:       3055     977    1327

  Fragments arranged by size: 

                3055    1327     977
 
AgeI A'CCGG_T

Cuts at:   4190    4190 
   Size:       5359
 
AhdI GACnn_n'nnGTC

Cuts at:   4088    4088 
   Size:       5359
 
AleI CACnn'nnGTG

Cuts at:   1166    2231    2324    1166 
   Size:       1065      93    4201

  Fragments arranged by size: 

                4201    1065      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2249    1184 
   Size:       1065    4294
 
AlwNI CAG_nnn'CTG

Cuts at:   3102    4674    3102 
   Size:       1572    3787
 
ApaI G_GGCC'C

Cuts at:   2339    2339 
   Size:       5359
 
ApaLI G'TGCA_C

Cuts at:   2314    3416    4572    2314 
   Size:       1102    1156    3101

  Fragments arranged by size: 

                3101    1156    1102
 
ApoI r'AATT_y

Cuts at:   1493    2668    2894    3046    5155    1493 
   Size:       1175     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1175     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3913     160 
   Size:        872    2881    1606

  Fragments arranged by size: 

                2881    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5359
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2332    2684    1870 
   Size:         73     389     352    4545

  Fragments arranged by size: 

                4545     389     352      73
 
BamHI G'GATC_C

Cuts at:   2689    3056    2689 
   Size:        367    4992
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2555    2680    4135     618 
   Size:        325     217     627     768     125    1455    1842

  Fragments arranged by size: 

                1842    1455     768     627     325     217     125
 
BanII G_rGCy'C

Cuts at:   1391    2339    2678    1391 
   Size:        948     339    4072

  Fragments arranged by size: 

                4072     948     339
 
BbeI G_GCGC'C

Cuts at:   2559    2559 
   Size:       5359
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4388
 
BbvCI CC'TCA_GC

Cuts at:   2585    2585 
   Size:       5359
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3550    3584    1903 
   Size:         34    1613      34    3678

  Fragments arranged by size: 

                3678    1613      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3251    4461    3251 
   Size:       1210    4149
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5359
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2052    2092    2617 
   Size:        107     122      71     631     605     380      40     525

Cuts at:   2617    3970     136 
   Size:       1353    1525

  Fragments arranged by size: 

                1525    1353     631     605     525     380     122     107
                  71      40
 
BglII A'GATC_T

Cuts at:   5354    5354 
   Size:       5359
 
BlpI GC'TnA_GC

Cuts at:   2776    2776 
   Size:       5359
 
Bme1580I G_kGCm'C

Cuts at:   1790    2187    2318    2339    2463    3420    4576    1790 
   Size:        397     131      21     124     957    1156    2573

  Fragments arranged by size: 

                2573    1156     957     397     131     124      21
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5359
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3090    4048     442 
   Size:        416      53      83     399      24    1673     958    1753

  Fragments arranged by size: 

                1753    1673     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5359
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2390    2390 
   Size:       5359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4019    1239 
   Size:       2780    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2585    2585 
   Size:       5359
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3484    4349    4647    3484 
   Size:        865     298    4196

  Fragments arranged by size: 

                4196     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4022     882 
   Size:        617    2523    2219

  Fragments arranged by size: 

                2523    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3150     493 
   Size:        711    1946    2702

  Fragments arranged by size: 

                2702    1946     711
 
BsaBI GATnn'nnATC

Cuts at:   5353    5353 
   Size:       5359
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2556    3166    3548    4183 
   Size:         53      83     186     936    1023     610     382     635

Cuts at:   4183     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1023     936     635     610     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3792    4190    4464    4611    5022    3792 
   Size:        398     274     147     411    4129

  Fragments arranged by size: 

                4129     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5134

  Fragments arranged by size: 

                5134     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5359
 
BseYI C'CCAG_C

Cuts at:   2055    2482    2505    4562    5058    2055 
   Size:        427      23    2057     496    2356

  Fragments arranged by size: 

                2356    2057     496     427      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2192    2255    2551    1163 
   Size:        573     456      63     296    3971

  Fragments arranged by size: 

                3971     573     456     296      63
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2575    2809    3570    4598    1540 
   Size:        275     186     574     234     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     574     275     234     186
 
BsiHKAI G_wGCw'C

Cuts at:   2180    2228    2318    2376    3420    3505    4576    2180 
   Size:         48      90      58    1044      85    1071    2963

  Fragments arranged by size: 

                2963    1071    1044      90      85      58      48
 
BsmI GAATG_Cn'

Cuts at:   2870    2963    2870 
   Size:         93    5266
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2180    2187    2228    2318    2339    2376    2463 
   Size:        399     390       7      41      90      21      37      87

Cuts at:   2463    2678    3420    3505    4576    1391 
   Size:        215     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     390     215      90      87
                  85      41      37      21       7
 
BspEI T'CCGG_A

Cuts at:   5022    5022 
   Size:       5359
 
BspHI T'CATG_A

Cuts at:   3248    4978    3248 
   Size:       1730    3629
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2086    2330    2714     844 
   Size:       1242     244     384    3489

  Fragments arranged by size: 

                3489    1242     384     244
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3246    1394 
   Size:        429    1423    3507

  Fragments arranged by size: 

                3507    1423     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3854    4028      65 
   Size:       1183    2606     174    1396

  Fragments arranged by size: 

                2606    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2570    2630    4003    4190    1700 
   Size:        110     760      60    1373     187    2869

  Fragments arranged by size: 

                2869    1373     760     187     110      60
 
BsrGI T'GTAC_A

Cuts at:     96    2496      96 
   Size:       2400    2959
 
BssHII G'CGCG_C

Cuts at:   1925    2307    1925 
   Size:        382    4977
 
BssSI C'ACGA_G

Cuts at:   2402    3419    4431    2402 
   Size:       1017    1012    3330

  Fragments arranged by size: 

                3330    1017    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2322     794 
   Size:       1528    3831
 
BstBI TT'CG_AA

Cuts at:   4163    4163 
   Size:       5359
 
BstEII G'GTnAC_C

Cuts at:   1184    2098    1184 
   Size:        914    4445
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2326    2415    2326 
   Size:         89    5270
 
BstYI r'GATC_y

Cuts at:   1972    2025    2689    3056    3441    3458    4910    4996    5008 
   Size:         53     664     367     385      17    1452      86      12

Cuts at:   5008    5354    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     664     385     367     346      86      53
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2612    2622     513 
   Size:        552     939     608      10    3250

  Fragments arranged by size: 

                3250     939     608     552      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4620

  Fragments arranged by size: 

                4620     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2198    2318    2875    3669    3689    2198 
   Size:        120     557     794      20    3868

  Fragments arranged by size: 

                3868     794     557     120      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5359
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5324
 
DraI TTT'AAA

Cuts at:   2663    2663 
   Size:       5359
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4366     809 
   Size:        507    3050    1802

  Fragments arranged by size: 

                3050    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2340    2511    2572 
   Size:         54    1425      50     275      18     510     171      61

Cuts at:   2572    2625    2806    3695    5324       8 
   Size:         53     181     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     510     275     181     171      61
                  54      53      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2572    2806    1537 
   Size:        275     760     234    4090

  Fragments arranged by size: 

                4090     760     275     234
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2294    3289    1353 
   Size:        941     995    3423

  Fragments arranged by size: 

                3423     995     941
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3946    4320    4466     387 
   Size:       1064     138    2357     374     146    1280

  Fragments arranged by size: 

                2357    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3422    4019    4806    5074    1239 
   Size:        393    1790     597     787     268    1524

  Fragments arranged by size: 

                1790    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2668    3046    2668 
   Size:        378    4981
 
FspI TGC'GCA

Cuts at:   3865    3865 
   Size:       5359
 
HaeII r_GCGC'y

Cuts at:   1678    2252    2265    2402    2495    2559    2612    4506    1678 
   Size:        574      13     137      93      64      53    1894    2531

  Fragments arranged by size: 

                2531    1894     574     137      93      64      53      13
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4000    4032    4074    4106    1373 
   Size:         32     135      32    2428      32      42      32    2626

  Fragments arranged by size: 

                2626    2428     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2703    2949     669 
   Size:        988     281     765     246    3079

  Fragments arranged by size: 

                3079     988     765     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5359
 
HpaI GTT'AAC

Cuts at:   2949    2949 
   Size:       5359
 
KasI G'GCGC_C

Cuts at:   2555    2555 
   Size:       5359
 
KpnI G_GTAC'C

Cuts at:   2684    2684 
   Size:       5359
 
MfeI C'AATT_G

Cuts at:   2958    2958 
   Size:       5359
 
MluI A'CGCG_T

Cuts at:   5235    5235 
   Size:       5359
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2001    4473    4657    1305 
   Size:        696    2472     184    2007

  Fragments arranged by size: 

                2472    2007     696     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3537

  Fragments arranged by size: 

                3537    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2231    2324    3317    3676    3835     518 
   Size:        648    1065      93     993     359     159    2042

  Fragments arranged by size: 

                2042    1065     993     648     359     159      93
 
MspA1I CmG'CkG

Cuts at:   1606    2614    2775    3452    4600    4845    1606 
   Size:       1008     161     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1008     677     245     161
 
NarI GG'CG_CC

Cuts at:   2556    2556 
   Size:       5359
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3868

  Fragments arranged by size: 

                3868     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5359
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5359
 
NotI GC'GGCC_GC

Cuts at:   2806    2806 
   Size:       5359
 
NspI r_CATG'y

Cuts at:   1132    1229    2191    2717    4262    1132 
   Size:         97     962     526    1545    2229

  Fragments arranged by size: 

                2229    1545     962     526      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5359
 
PciI A'CATG_T

Cuts at:   4258    4258 
   Size:       5359
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5359
 
PfoI T'CCnGG_A

Cuts at:   2109    2109 
   Size:       5359
 
PmeI GTTT'AAAC

Cuts at:   2663    2663 
   Size:       5359
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3428    3460    4941    4973    3428 
   Size:         32    1481      32    3814

  Fragments arranged by size: 

                3814    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5359
 
PsiI TTA'TAA

Cuts at:   2929    2929 
   Size:       5359
 
PspOMI G'GGCC_C

Cuts at:   2335    2335 
   Size:       5359
 
PstI C_TGCA'G

Cuts at:    830    2711     830 
   Size:       1881    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5359
 
SacII CC_GC'GG

Cuts at:   2615    2615 
   Size:       5359
 
SalI G'TCGA_C

Cuts at:   1936    2701    1936 
   Size:        765    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2294    2294 
   Size:       5359
 
SbfI CC_TGCA'GG

Cuts at:   2711    2711 
   Size:       5359
 
ScaI AGT'ACT

Cuts at:   2603    3607    5319    2603 
   Size:       1004    1712    2643

  Fragments arranged by size: 

                2643    1712    1004
 
SfcI C'TryA_G

Cuts at:    826    1045    2707    3842    4523    4714     826 
   Size:        219    1662    1135     681     191    1471

  Fragments arranged by size: 

                1662    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2557    2557 
   Size:       5359
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5359
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2630    1700 
   Size:        930    4429
 
SmaI CCC'GGG

Cuts at:   1872    2334    2686    1872 
   Size:        462     352    4545

  Fragments arranged by size: 

                4545     462     352
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3463    4364    4626     820 
   Size:        197     926    1520     901     262    1553

  Fragments arranged by size: 

                1553    1520     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3150     493 
   Size:       2657    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5359
 
SphI G_CATG'C

Cuts at:   1132    2717    1132 
   Size:       1585    3774
 
SspI AAT'ATT

Cuts at:      5      52    3283       5 
   Size:         47    3231    2081

  Fragments arranged by size: 

                3231    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5359
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2343    2798     513 
   Size:        552     939     339     455    3074

  Fragments arranged by size: 

                3074     939     552     455     339
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3386    3403    3556    3741    4186     325 
   Size:       1255    1806      17     153     185     445    1498

  Fragments arranged by size: 

                1806    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2496    2601    3605 
   Size:        274      80      33      51    1443     519     105    1004

Cuts at:   3605    5317      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1004     519     274     138     105      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2560    2592    5319    5351    2560 
   Size:         32    2727      32    2568

  Fragments arranged by size: 

                2727    2568      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5359
 
XbaI T'CTAG_A

Cuts at:   2695    2695 
   Size:       5359
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2350    2350 
   Size:       5359
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5359
 
XmaI C'CCGG_G

Cuts at:   1870    2332    2684    1870 
   Size:        462     352    4545

  Fragments arranged by size: 

                4545     462     352
 
XmnI GAAnn'nnTTC

Cuts at:   3488    3488 
   Size:       5359
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3167     276 
   Size:         53      83     186     936    1633    2468

  Fragments arranged by size: 

                2468    1633     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI    SacII
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI    BfrBI    BsiWI    BsmBI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI
    PspXI     PsrI    PvuII    RsrII     SacI    SanDI    SexAI     SfiI
     SrfI     SwaI
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