(Circular) (Six-base) MAPSORT of: pFN21AE0494.seq Check: 9473 from: 1 to: 5359 pFN21AE0494 5359 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2086 2330 2086 Size: 244 5115 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3169 278 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 1937 Size: 765 4594 Acc65I G'GTAC_C Cuts at: 2680 2680 Size: 5359 AclI AA'CG_TT Cuts at: 1563 3486 3859 1563 Size: 1923 373 3063 Fragments arranged by size: 3063 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3422 4806 5074 1632 Size: 1790 1384 268 1917 Fragments arranged by size: 1917 1790 1384 268 AfeI AGC'GCT Cuts at: 2493 2493 Size: 5359 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5162 AflIII A'CryG_T Cuts at: 1203 4258 5235 1203 Size: 3055 977 1327 Fragments arranged by size: 3055 1327 977 AgeI A'CCGG_T Cuts at: 4190 4190 Size: 5359 AhdI GACnn_n'nnGTC Cuts at: 4088 4088 Size: 5359 AleI CACnn'nnGTG Cuts at: 1166 2231 2324 1166 Size: 1065 93 4201 Fragments arranged by size: 4201 1065 93 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2249 1184 Size: 1065 4294 AlwNI CAG_nnn'CTG Cuts at: 3102 4674 3102 Size: 1572 3787 ApaI G_GGCC'C Cuts at: 2339 2339 Size: 5359 ApaLI G'TGCA_C Cuts at: 2314 3416 4572 2314 Size: 1102 1156 3101 Fragments arranged by size: 3101 1156 1102 ApoI r'AATT_y Cuts at: 1493 2668 2894 3046 5155 1493 Size: 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 3913 160 Size: 872 2881 1606 Fragments arranged by size: 2881 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 AvaI C'yCGr_G Cuts at: 1870 1943 2332 2684 1870 Size: 73 389 352 4545 Fragments arranged by size: 4545 389 352 73 BamHI G'GATC_C Cuts at: 2689 3056 2689 Size: 367 4992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 2680 4135 618 Size: 325 217 627 768 125 1455 1842 Fragments arranged by size: 1842 1455 768 627 325 217 125 BanII G_rGCy'C Cuts at: 1391 2339 2678 1391 Size: 948 339 4072 Fragments arranged by size: 4072 948 339 BbeI G_GCGC'C Cuts at: 2559 2559 Size: 5359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4388 BbvCI CC'TCA_GC Cuts at: 2585 2585 Size: 5359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3550 3584 1903 Size: 34 1613 34 3678 Fragments arranged by size: 3678 1613 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3251 4461 3251 Size: 1210 4149 BclI T'GATC_A Cuts at: 1548 1548 Size: 5359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 2092 2617 Size: 107 122 71 631 605 380 40 525 Cuts at: 2617 3970 136 Size: 1353 1525 Fragments arranged by size: 1525 1353 631 605 525 380 122 107 71 40 BglII A'GATC_T Cuts at: 5354 5354 Size: 5359 BlpI GC'TnA_GC Cuts at: 2776 2776 Size: 5359 Bme1580I G_kGCm'C Cuts at: 1790 2187 2318 2339 2463 3420 4576 1790 Size: 397 131 21 124 957 1156 2573 Fragments arranged by size: 2573 1156 957 397 131 124 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 4048 442 Size: 416 53 83 399 24 1673 958 1753 Fragments arranged by size: 1753 1673 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5359 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2390 2390 Size: 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4019 1239 Size: 2780 2579 Bpu10I CC'TnA_GC Cuts at: 2585 2585 Size: 5359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3484 4349 4647 3484 Size: 865 298 4196 Fragments arranged by size: 4196 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4022 882 Size: 617 2523 2219 Fragments arranged by size: 2523 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 493 Size: 711 1946 2702 Fragments arranged by size: 2702 1946 711 BsaBI GATnn'nnATC Cuts at: 5353 5353 Size: 5359 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2556 3166 3548 4183 Size: 53 83 186 936 1023 610 382 635 Cuts at: 4183 275 Size: 1451 Fragments arranged by size: 1451 1023 936 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3792 4190 4464 4611 5022 3792 Size: 398 274 147 411 4129 Fragments arranged by size: 4129 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5134 Fragments arranged by size: 5134 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5359 BseYI C'CCAG_C Cuts at: 2055 2482 2505 4562 5058 2055 Size: 427 23 2057 496 2356 Fragments arranged by size: 2356 2057 496 427 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 2255 2551 1163 Size: 573 456 63 296 3971 Fragments arranged by size: 3971 573 456 296 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2575 2809 3570 4598 1540 Size: 275 186 574 234 761 1028 2301 Fragments arranged by size: 2301 1028 761 574 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2180 2228 2318 2376 3420 3505 4576 2180 Size: 48 90 58 1044 85 1071 2963 Fragments arranged by size: 2963 1071 1044 90 85 58 48 BsmI GAATG_Cn' Cuts at: 2870 2963 2870 Size: 93 5266 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2187 2228 2318 2339 2376 2463 Size: 399 390 7 41 90 21 37 87 Cuts at: 2463 2678 3420 3505 4576 1391 Size: 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 215 90 87 85 41 37 21 7 BspEI T'CCGG_A Cuts at: 5022 5022 Size: 5359 BspHI T'CATG_A Cuts at: 3248 4978 3248 Size: 1730 3629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2086 2330 2714 844 Size: 1242 244 384 3489 Fragments arranged by size: 3489 1242 384 244 BsrBI CCG'CTC Cuts at: 1394 1823 3246 1394 Size: 429 1423 3507 Fragments arranged by size: 3507 1423 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 4028 65 Size: 1183 2606 174 1396 Fragments arranged by size: 2606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2570 2630 4003 4190 1700 Size: 110 760 60 1373 187 2869 Fragments arranged by size: 2869 1373 760 187 110 60 BsrGI T'GTAC_A Cuts at: 96 2496 96 Size: 2400 2959 BssHII G'CGCG_C Cuts at: 1925 2307 1925 Size: 382 4977 BssSI C'ACGA_G Cuts at: 2402 3419 4431 2402 Size: 1017 1012 3330 Fragments arranged by size: 3330 1017 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2322 794 Size: 1528 3831 BstBI TT'CG_AA Cuts at: 4163 4163 Size: 5359 BstEII G'GTnAC_C Cuts at: 1184 2098 1184 Size: 914 4445 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2415 2326 Size: 89 5270 BstYI r'GATC_y Cuts at: 1972 2025 2689 3056 3441 3458 4910 4996 5008 Size: 53 664 367 385 17 1452 86 12 Cuts at: 5008 5354 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 664 385 367 346 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2612 2622 513 Size: 552 939 608 10 3250 Fragments arranged by size: 3250 939 608 552 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4620 Fragments arranged by size: 4620 683 56 BtsI GCAGTG_nn' Cuts at: 2198 2318 2875 3669 3689 2198 Size: 120 557 794 20 3868 Fragments arranged by size: 3868 794 557 120 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5359 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5324 DraI TTT'AAA Cuts at: 2663 2663 Size: 5359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4366 809 Size: 507 3050 1802 Fragments arranged by size: 3050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2511 2572 Size: 54 1425 50 275 18 510 171 61 Cuts at: 2572 2625 2806 3695 5324 8 Size: 53 181 889 1629 43 Fragments arranged by size: 1629 1425 889 510 275 181 171 61 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2572 2806 1537 Size: 275 760 234 4090 Fragments arranged by size: 4090 760 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 3289 1353 Size: 941 995 3423 Fragments arranged by size: 3423 995 941 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3946 4320 4466 387 Size: 1064 138 2357 374 146 1280 Fragments arranged by size: 2357 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3422 4019 4806 5074 1239 Size: 393 1790 597 787 268 1524 Fragments arranged by size: 1790 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2668 3046 2668 Size: 378 4981 FspI TGC'GCA Cuts at: 3865 3865 Size: 5359 HaeII r_GCGC'y Cuts at: 1678 2252 2265 2402 2495 2559 2612 4506 1678 Size: 574 13 137 93 64 53 1894 2531 Fragments arranged by size: 2531 1894 574 137 93 64 53 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373 Size: 32 135 32 2428 32 42 32 2626 Fragments arranged by size: 2626 2428 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2949 669 Size: 988 281 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5359 HpaI GTT'AAC Cuts at: 2949 2949 Size: 5359 KasI G'GCGC_C Cuts at: 2555 2555 Size: 5359 KpnI G_GTAC'C Cuts at: 2684 2684 Size: 5359 MfeI C'AATT_G Cuts at: 2958 2958 Size: 5359 MluI A'CGCG_T Cuts at: 5235 5235 Size: 5359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 4473 4657 1305 Size: 696 2472 184 2007 Fragments arranged by size: 2472 2007 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3537 Fragments arranged by size: 3537 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2324 3317 3676 3835 518 Size: 648 1065 93 993 359 159 2042 Fragments arranged by size: 2042 1065 993 648 359 159 93 MspA1I CmG'CkG Cuts at: 1606 2614 2775 3452 4600 4845 1606 Size: 1008 161 677 1148 245 2120 Fragments arranged by size: 2120 1148 1008 677 245 161 NarI GG'CG_CC Cuts at: 2556 2556 Size: 5359 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3868 Fragments arranged by size: 3868 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5359 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5359 NotI GC'GGCC_GC Cuts at: 2806 2806 Size: 5359 NspI r_CATG'y Cuts at: 1132 1229 2191 2717 4262 1132 Size: 97 962 526 1545 2229 Fragments arranged by size: 2229 1545 962 526 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5359 PciI A'CATG_T Cuts at: 4258 4258 Size: 5359 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5359 PfoI T'CCnGG_A Cuts at: 2109 2109 Size: 5359 PmeI GTTT'AAAC Cuts at: 2663 2663 Size: 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3460 4941 4973 3428 Size: 32 1481 32 3814 Fragments arranged by size: 3814 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5359 PsiI TTA'TAA Cuts at: 2929 2929 Size: 5359 PspOMI G'GGCC_C Cuts at: 2335 2335 Size: 5359 PstI C_TGCA'G Cuts at: 830 2711 830 Size: 1881 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5359 SacII CC_GC'GG Cuts at: 2615 2615 Size: 5359 SalI G'TCGA_C Cuts at: 1936 2701 1936 Size: 765 4594 SapI GCTCTTCn'nnn_ Cuts at: 2294 2294 Size: 5359 SbfI CC_TGCA'GG Cuts at: 2711 2711 Size: 5359 ScaI AGT'ACT Cuts at: 2603 3607 5319 2603 Size: 1004 1712 2643 Fragments arranged by size: 2643 1712 1004 SfcI C'TryA_G Cuts at: 826 1045 2707 3842 4523 4714 826 Size: 219 1662 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2557 2557 Size: 5359 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 SgrAI Cr'CCGG_yG Cuts at: 1700 2630 1700 Size: 930 4429 SmaI CCC'GGG Cuts at: 1872 2334 2686 1872 Size: 462 352 4545 Fragments arranged by size: 4545 462 352 SmlI C'TyrA_G Cuts at: 820 1017 1943 3463 4364 4626 820 Size: 197 926 1520 901 262 1553 Fragments arranged by size: 1553 1520 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3150 493 Size: 2657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5359 SphI G_CATG'C Cuts at: 1132 2717 1132 Size: 1585 3774 SspI AAT'ATT Cuts at: 5 52 3283 5 Size: 47 3231 2081 Fragments arranged by size: 3231 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5359 StyI C'CwwG_G Cuts at: 513 1065 2004 2343 2798 513 Size: 552 939 339 455 3074 Fragments arranged by size: 3074 939 552 455 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 3403 3556 3741 4186 325 Size: 1255 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 2601 3605 Size: 274 80 33 51 1443 519 105 1004 Cuts at: 3605 5317 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1004 519 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2560 2592 5319 5351 2560 Size: 32 2727 32 2568 Fragments arranged by size: 2727 2568 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5359 XbaI T'CTAG_A Cuts at: 2695 2695 Size: 5359 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5359 XmaI C'CCGG_G Cuts at: 1870 2332 2684 1870 Size: 462 352 4545 Fragments arranged by size: 4545 462 352 XmnI GAAnn'nnTTC Cuts at: 3488 3488 Size: 5359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3167 276 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI