Restriction map for
pFN21AB0998
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 469 AAATTT [GA]^AATT[CT]
ApoI 469 AAATTT [GA]^AATT[CT]
AspHI 618 GAGCTC G[AT]GC[AT]^C
AvaI 332 CCCGAG C^[CT]CG[GA]G
AvrII 36 CCTAGG C^CTAGG
BamHI 425 GGATCC G^GATCC
BanII 618 GAGCTC G[GA]GC[CT]^C
BbsI 316 GTCTTC GAAGAC(2/6)
BfaI 37 CTAG C^TAG
BlnI 36 CCTAGG C^CTAGG
BpuAI 316 GTCTTC GAAGAC(2/6)
BseRI 270 CTCCTC GAGGAG(10/8)
BsiHKAI 618 GAGCTC G[AT]GC[AT]^C
BsmFI 434 GGGAC GGGAC(10/14)
BsoBI 332 CCCGAG C^[CT]CG[GA]G
BspMI 358 GCAGGT ACCTGC(4/8)
BstAPI 302 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 425 GGATCC [GA]^GATC[CT]
DpnII 426 GATC ^GATC
DraII 143 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 183 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 143 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 220 CCCGC CCCGC(4/6)
HincII 635 GTCAAC GT[CT]^[GA]AC
HindII 635 GTCAAC GT[CT]^[GA]AC
MaeI 37 CTAG C^TAG
MboI 426 GATC ^GATC
MflI 425 GGATCC [GA]^GATC[CT]
MslI 246 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 426 GATC ^GATC
PleI 365 GAGTC GAGTC(4/5)
PvuII 84 CAGCTG CAG^CTG
SacI 618 GAGCTC GAGCT^C
Sau3AI 426 GATC ^GATC
XcmI 338 CCAAAGACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 425 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 221 CCGC CCGC(-3/-1)
352 GCGG
AlwI 426 GATCC GGATC(4/5)
425 GGATC
BanI 109 GGTGCC G^G[CT][GA]CC
453 GGTGCC
BbvI 75 GCTGC GCAGC(8/12)
235 GCTGC
BmyI 57 GGGCAC G[AGT]GC[ACT]^C
618 GAGCTC
Bsp1286I 57 GGGCAC G[AGT]GC[ACT]^C
618 GAGCTC
Cfr13I 144 GGGCC G^G[ACGT]CC
213 GGGCC
Fnu4HI 75 GCTGC GC^[ACGT]GC
235 GCTGC
ItaI 75 GCTGC GC^[ACGT]GC
235 GCTGC
MspA1I 84 CAGCTG C[AC]G^C[GT]G
221 CCGCTG
PflMI 18 CCAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
625 CCAAGGCCTGG
Sau96I 144 GGGCC G^G[ACGT]CC
213 GGGCC
SfcI 198 CTATAG C^T[GA][CT]AG
669 CTACAG
TseI 75 GCTGC G^C[AT]GC
235 GCTGC
Tsp509I 470 AATT ^AATT
664 AATT
Van91I 18 CCAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
625 CCAAGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I EcoRII
EcoT14I FokI HaeIII HinfI HphI
MaeIII MboII MnlI MvaI MwoI
NlaIII NlaIV ScrFI SfaNI StuI
StyI TfiI Tsp45I TspRI