(Circular) (Six-base) MAPSORT of: pFN21AB0998.seq Check: 7854 from: 1 to: 5395 pFN21AB0998 5395 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2354 2354 Size: 5395 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3205 278 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2738 1937 Size: 801 4594 Acc65I G'GTAC_C Cuts at: 2716 2716 Size: 5395 AclI AA'CG_TT Cuts at: 1563 3522 3895 1563 Size: 1959 373 3063 Fragments arranged by size: 3063 1959 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3458 4842 5110 1632 Size: 1826 1384 268 1917 Fragments arranged by size: 1917 1826 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5198 AflIII A'CryG_T Cuts at: 1203 4294 5271 1203 Size: 3091 977 1327 Fragments arranged by size: 3091 1327 977 AgeI A'CCGG_T Cuts at: 4226 4226 Size: 5395 AhdI GACnn_n'nnGTC Cuts at: 4124 4124 Size: 5395 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5395 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2086 2118 2086 Size: 32 5363 AlwNI CAG_nnn'CTG Cuts at: 3138 4710 3138 Size: 1572 3823 ApaLI G'TGCA_C Cuts at: 3452 4608 3452 Size: 1156 4239 ApoI r'AATT_y Cuts at: 1493 2474 2704 2930 3082 5191 1493 Size: 981 230 226 152 2109 1697 Fragments arranged by size: 2109 1697 981 230 226 152 AseI AT'TA_AT Cuts at: 160 1032 3949 160 Size: 872 2917 1606 Fragments arranged by size: 2917 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 AvaI C'yCGr_G Cuts at: 1870 1943 2337 2720 1870 Size: 73 394 383 4545 Fragments arranged by size: 4545 394 383 73 AvrII C'CTAG_G Cuts at: 2041 2041 Size: 5395 BamHI G'GATC_C Cuts at: 2430 2725 3092 2430 Size: 295 367 4733 Fragments arranged by size: 4733 367 295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2458 2716 4171 618 Size: 325 217 627 327 344 258 1455 1842 Fragments arranged by size: 1842 1455 627 344 327 325 258 217 BanII G_rGCy'C Cuts at: 1391 2627 2714 1391 Size: 1236 87 4072 Fragments arranged by size: 4072 1236 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2314 928 Size: 971 415 4009 Fragments arranged by size: 4009 971 415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3586 3620 1903 Size: 34 1649 34 3678 Fragments arranged by size: 3678 1649 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3287 4497 3287 Size: 1210 4185 BclI T'GATC_A Cuts at: 1548 1548 Size: 5395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4006 136 Size: 107 122 71 631 605 2334 1525 Fragments arranged by size: 2334 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5390 5390 Size: 5395 BlpI GC'TnA_GC Cuts at: 2812 2812 Size: 5395 Bme1580I G_kGCm'C Cuts at: 1790 2066 3456 4612 1790 Size: 276 1390 1156 2573 Fragments arranged by size: 2573 1390 1156 276 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2185 3126 4084 Size: 416 53 83 399 24 768 941 958 Cuts at: 4084 442 Size: 1753 Fragments arranged by size: 1753 958 941 768 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4055 1239 Size: 2816 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3520 4385 4683 3520 Size: 865 298 4232 Fragments arranged by size: 4232 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4058 882 Size: 617 2559 2219 Fragments arranged by size: 2559 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3186 493 Size: 711 1982 2702 Fragments arranged by size: 2702 1982 711 BsaBI GATnn'nnATC Cuts at: 5389 5389 Size: 5395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3202 3584 4219 275 Size: 53 83 186 936 1669 382 635 1451 Fragments arranged by size: 1669 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3828 4226 4500 4647 5058 3828 Size: 398 274 147 411 4165 Fragments arranged by size: 4165 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5170 Fragments arranged by size: 5170 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2266 1186 Size: 1080 4315 BseYI C'CCAG_C Cuts at: 2087 4598 5094 2087 Size: 2511 496 2388 Fragments arranged by size: 2511 2388 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4822 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2845 3606 4634 1540 Size: 275 186 844 761 1028 2301 Fragments arranged by size: 2301 1028 844 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2627 3456 3541 4612 2627 Size: 829 85 1071 3410 Fragments arranged by size: 3410 1071 829 85 BsmI GAATG_Cn' Cuts at: 2906 2999 2906 Size: 93 5302 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2627 2714 3456 3541 4612 1391 Size: 399 276 561 87 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 399 276 87 85 BspEI T'CCGG_A Cuts at: 5058 5058 Size: 5395 BspHI T'CATG_A Cuts at: 3284 5014 3284 Size: 1730 3665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2354 2750 844 Size: 1510 396 3489 Fragments arranged by size: 3489 1510 396 BsrBI CCG'CTC Cuts at: 1394 1823 3282 1394 Size: 429 1459 3507 Fragments arranged by size: 3507 1459 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3890 4064 65 Size: 1183 2642 174 1396 Fragments arranged by size: 2642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4039 4226 1700 Size: 110 2229 187 2869 Fragments arranged by size: 2869 2229 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5395 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5395 BssSI C'ACGA_G Cuts at: 3455 4467 3455 Size: 1012 4383 BstAPI GCAn_nnn'nTGC Cuts at: 794 2313 794 Size: 1519 3876 BstBI TT'CG_AA Cuts at: 4199 4199 Size: 5395 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5395 BstYI r'GATC_y Cuts at: 1972 2430 2725 3092 3477 3494 4946 5032 5044 Size: 458 295 367 385 17 1452 86 12 Cuts at: 5044 5390 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 458 385 367 346 295 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3904 Fragments arranged by size: 3904 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4656 Fragments arranged by size: 4656 683 56 BtsI GCAGTG_nn' Cuts at: 2911 3705 3725 2911 Size: 794 20 4581 Fragments arranged by size: 4581 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5360 DraI TTT'AAA Cuts at: 2699 2699 Size: 5395 DraIII CAC_nnn'GTG Cuts at: 2193 2193 Size: 5395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4402 809 Size: 507 3086 1802 Fragments arranged by size: 3086 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2842 3731 5360 Size: 54 1425 50 275 18 1012 889 1629 Cuts at: 5360 8 Size: 43 Fragments arranged by size: 1629 1425 1012 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2842 1537 Size: 275 1030 4090 Fragments arranged by size: 4090 1030 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3325 1353 Size: 1972 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3982 4356 4502 387 Size: 1064 138 2393 374 146 1280 Fragments arranged by size: 2393 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2625 2625 Size: 5395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3458 4055 4842 5110 1239 Size: 393 1826 597 787 268 1524 Fragments arranged by size: 1826 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2149 2149 Size: 5395 EcoRI G'AATT_C Cuts at: 2704 3082 2704 Size: 378 5017 FspI TGC'GCA Cuts at: 3901 3901 Size: 5395 HaeII r_GCGC'y Cuts at: 1678 4542 1678 Size: 2864 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 2432 2464 4036 Size: 32 135 32 732 32 96 32 1572 Cuts at: 4036 4068 4110 4142 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1572 732 135 96 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2642 2739 2985 669 Size: 988 281 704 97 246 3079 Fragments arranged by size: 3079 988 704 281 246 97 HindIII A'AGCT_T Cuts at: 748 748 Size: 5395 HpaI GTT'AAC Cuts at: 2985 2985 Size: 5395 KpnI G_GTAC'C Cuts at: 2720 2720 Size: 5395 MfeI C'AATT_G Cuts at: 2994 2994 Size: 5395 MluI A'CGCG_T Cuts at: 5271 5271 Size: 5395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2047 2604 4509 4693 1305 Size: 742 557 1905 184 2007 Fragments arranged by size: 2007 1905 742 557 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3573 Fragments arranged by size: 3573 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 3353 3712 3871 518 Size: 648 1089 1098 359 159 2042 Fragments arranged by size: 2042 1098 1089 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 2228 2811 3488 4636 4881 1606 Size: 485 137 583 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 485 245 137 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3904 Fragments arranged by size: 3904 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5395 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5395 NotI GC'GGCC_GC Cuts at: 2842 2842 Size: 5395 NspI r_CATG'y Cuts at: 1132 1229 2753 4298 1132 Size: 97 1524 1545 2229 Fragments arranged by size: 2229 1545 1524 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5395 PciI A'CATG_T Cuts at: 4294 4294 Size: 5395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2029 2636 1108 Size: 921 607 3867 Fragments arranged by size: 3867 921 607 PmeI GTTT'AAAC Cuts at: 2699 2699 Size: 5395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3464 3496 4977 5009 3464 Size: 32 1481 32 3850 Fragments arranged by size: 3850 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5395 PsiI TTA'TAA Cuts at: 2965 2965 Size: 5395 PstI C_TGCA'G Cuts at: 830 2747 830 Size: 1917 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5395 PvuII CAG'CTG Cuts at: 2091 2091 Size: 5395 SacI G_AGCT'C Cuts at: 2627 2627 Size: 5395 SalI G'TCGA_C Cuts at: 1936 2737 1936 Size: 801 4594 SbfI CC_TGCA'GG Cuts at: 2747 2747 Size: 5395 ScaI AGT'ACT Cuts at: 3643 5355 3643 Size: 1712 3683 SfcI C'TryA_G Cuts at: 826 1045 2203 2674 2743 3878 4559 4750 826 Size: 219 1158 471 69 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 681 471 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5395 SmaI CCC'GGG Cuts at: 1872 2722 1872 Size: 850 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3499 4400 4662 820 Size: 197 926 1556 901 262 1553 Fragments arranged by size: 1556 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3186 493 Size: 2693 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5395 SphI G_CATG'C Cuts at: 1132 2753 1132 Size: 1621 3774 SspI AAT'ATT Cuts at: 5 52 3319 5 Size: 47 3267 2081 Fragments arranged by size: 3267 2081 47 StuI AGG'CCT Cuts at: 1517 2046 2391 2635 1517 Size: 529 345 244 4277 Fragments arranged by size: 4277 529 345 244 StyI C'CwwG_G Cuts at: 513 1065 2004 2041 2145 2383 2630 2834 513 Size: 552 939 37 104 238 247 204 3074 Fragments arranged by size: 3074 939 552 247 238 204 104 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 3422 3439 3592 3777 4222 325 Size: 1255 624 1218 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1218 624 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3641 5353 96 Size: 274 80 33 51 1443 1664 1712 138 Fragments arranged by size: 1712 1664 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2149 2181 5355 5387 2149 Size: 32 3174 32 2157 Fragments arranged by size: 3174 2157 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5395 XbaI T'CTAG_A Cuts at: 2731 2731 Size: 5395 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 5395 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5395 XmaI C'CCGG_G Cuts at: 1870 2720 1870 Size: 850 4545 XmnI GAAnn'nnTTC Cuts at: 3524 3524 Size: 5395 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3203 276 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI