################################## # NO CUT ENZYMES ( 212 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MvnI MwoI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 57 GCGG CCGC(-3/-1) AclI 315 AACGTT AA^CGTT AfaI 139 GTAC GT^AC AlwNI 65 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 211 ATTAAT AT^TAAT AsnI 211 ATTAAT AT^TAAT Asp700I 109 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 376 GGTCC G^G[AT]CC BcnI 83 CCGGG CC^[GC]GG BfaI 92 CTAG C^TAG BpmI 7 CTGGAG CTGGAG(16/14) BsaJI 132 CCTTGG C^C[ACGT][ACGT]GG BsgI 34 GTGCAG GTGCAG(16/14) BsiYI 127 CCTTTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 127 CCTTTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 67 GTCCC GGGAC(10/14) BsrI 64 CCAGT ACTGG(1/-1) BssKI 83 CCGGG ^CC[ACGT]GG Cac8I 325 GCCTGC GC[ACGT]^[ACGT]GC EcoT14I 132 CCTTGG C^C[AT][AT]GG HaeIII 18 GGCC GG^CC HapII 83 CCGG C^CGG HpaII 83 CCGG C^CGG MaeI 92 CTAG C^TAG MaeII 316 ACGT A^CGT MnlI 177 CCTC CCTC(7/6) MspI 83 CCGG C^CGG NciI 83 CCGGG CC^[GC]GG PshBI 211 ATTAAT AT^TAAT Psp1406I 315 AACGTT AA^CGTT RsaI 139 GTAC GT^AC ScrFI 83 CCGGG CC^[ACGT]GG StyI 132 CCTTGG C^C[AT][AT]GG Tsp509I 356 AATT ^AATT XmnI 109 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BbvI 36 GCAGC GCAGC(8/12) 286 GCAGC Cfr13I 18 GGCCC G^G[ACGT]CC 376 GGTCC MseI 212 TTAA T^TAA 218 TTAA NlaIV 59 GGCTCC GG[ACGT]^[ACGT]CC 375 GGGTCC Sau96I 18 GGCCC G^G[ACGT]CC 376 GGTCC Tru9I 212 TTAA T^TAA 218 TTAA TseI 36 GCAGC G^C[AT]GC 286 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AluI Fnu4HI ItaI MboII