(Circular) (Six-base) MAPSORT of: pFN21AE6103.seq Check: 472 from: 1 to: 5110 pFN21AE6103 5110 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2920 278 Size: 53 83 186 936 1384 2468 Fragments arranged by size: 2468 1384 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2453 1937 Size: 516 4594 Acc65I G'GTAC_C Cuts at: 2431 2431 Size: 5110 AclI AA'CG_TT Cuts at: 1563 2321 3237 3610 1563 Size: 758 916 373 3063 Fragments arranged by size: 3063 916 758 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2414 3173 4557 4825 1632 Size: 446 336 759 1384 268 1917 Fragments arranged by size: 1917 1384 759 446 336 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4913 AflIII A'CryG_T Cuts at: 1203 4009 4986 1203 Size: 2806 977 1327 Fragments arranged by size: 2806 1327 977 AgeI A'CCGG_T Cuts at: 3941 3941 Size: 5110 AhdI GACnn_n'nnGTC Cuts at: 3839 3839 Size: 5110 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5110 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2064 1184 Size: 880 4230 AlwNI CAG_nnn'CTG Cuts at: 2075 2853 4425 2075 Size: 778 1572 2760 Fragments arranged by size: 2760 1572 778 ApaLI G'TGCA_C Cuts at: 3167 4323 3167 Size: 1156 3954 ApoI r'AATT_y Cuts at: 1493 2419 2645 2797 4906 1493 Size: 926 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 226 152 AseI AT'TA_AT Cuts at: 160 1032 2217 3664 160 Size: 872 1185 1447 1606 Fragments arranged by size: 1606 1447 1185 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5110 AvaI C'yCGr_G Cuts at: 1870 1943 2435 1870 Size: 73 492 4545 Fragments arranged by size: 4545 492 73 BamHI G'GATC_C Cuts at: 2440 2807 2440 Size: 367 4743 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2431 3886 618 Size: 325 217 627 644 1455 1842 Fragments arranged by size: 1842 1455 644 627 325 217 BanII G_rGCy'C Cuts at: 1391 2429 1391 Size: 1038 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3301 3335 1903 Size: 34 1364 34 3678 Fragments arranged by size: 3678 1364 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3002 4212 3002 Size: 1210 3900 BclI T'GATC_A Cuts at: 1548 1548 Size: 5110 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3721 136 Size: 107 122 71 631 605 2049 1525 Fragments arranged by size: 2049 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5105 5105 Size: 5110 BlpI GC'TnA_GC Cuts at: 2527 2527 Size: 5110 Bme1580I G_kGCm'C Cuts at: 1790 3171 4327 1790 Size: 1381 1156 2573 Fragments arranged by size: 2573 1381 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 2841 3799 Size: 416 53 83 399 24 646 778 958 Cuts at: 3799 442 Size: 1753 Fragments arranged by size: 1753 958 778 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5110 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 3770 1239 Size: 794 1737 2579 Fragments arranged by size: 2579 1737 794 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3235 4100 4398 3235 Size: 865 298 3947 Fragments arranged by size: 3947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3773 882 Size: 617 2274 2219 Fragments arranged by size: 2274 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2901 493 Size: 711 1697 2702 Fragments arranged by size: 2702 1697 711 BsaBI GATnn'nnATC Cuts at: 5104 5104 Size: 5110 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2917 3299 3934 275 Size: 53 83 186 936 1384 382 635 1451 Fragments arranged by size: 1451 1384 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3543 3941 4215 4362 4773 3543 Size: 398 274 147 411 3880 Fragments arranged by size: 3880 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2361 2391 1178 Size: 30 165 30 958 30 3897 Fragments arranged by size: 3897 958 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5110 BseYI C'CCAG_C Cuts at: 4313 4809 4313 Size: 496 4614 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 1163 Size: 573 324 4213 Fragments arranged by size: 4213 573 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2560 3321 4349 1540 Size: 275 186 559 761 1028 2301 Fragments arranged by size: 2301 1028 761 559 275 186 BsiHKAI G_wGCw'C Cuts at: 3171 3256 4327 3171 Size: 85 1071 3954 Fragments arranged by size: 3954 1071 85 BsmI GAATG_Cn' Cuts at: 2621 2714 2621 Size: 93 5017 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2429 3171 3256 4327 1391 Size: 399 639 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 399 85 BspEI T'CCGG_A Cuts at: 4773 4773 Size: 5110 BspHI T'CATG_A Cuts at: 2999 4729 2999 Size: 1730 3380 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2465 844 Size: 1621 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2997 1394 Size: 429 1174 3507 Fragments arranged by size: 3507 1174 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3605 3779 65 Size: 1183 2357 174 1396 Fragments arranged by size: 2357 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3754 3941 1700 Size: 110 1944 187 2869 Fragments arranged by size: 2869 1944 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5110 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5110 BssSI C'ACGA_G Cuts at: 3170 4182 3170 Size: 1012 4098 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5110 BstBI TT'CG_AA Cuts at: 3914 3914 Size: 5110 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5110 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5110 BstYI r'GATC_y Cuts at: 1972 2440 2807 3192 3209 4661 4747 4759 5105 Size: 468 367 385 17 1452 86 12 346 Cuts at: 5105 1972 Size: 1977 Fragments arranged by size: 1977 1452 468 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3619 Fragments arranged by size: 3619 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2349 487 Size: 683 56 1123 3248 Fragments arranged by size: 3248 1123 683 56 BtsI GCAGTG_nn' Cuts at: 2626 3420 3440 2626 Size: 794 20 4296 Fragments arranged by size: 4296 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5110 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5075 DraI TTT'AAA Cuts at: 2414 2414 Size: 5110 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4117 809 Size: 507 2801 1802 Fragments arranged by size: 2801 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2557 3446 5075 Size: 54 1425 50 275 18 727 889 1629 Cuts at: 5075 8 Size: 43 Fragments arranged by size: 1629 1425 889 727 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2557 1537 Size: 275 745 4090 Fragments arranged by size: 4090 745 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3040 1353 Size: 1687 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3697 4071 4217 387 Size: 1064 138 2108 374 146 1280 Fragments arranged by size: 2108 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2078 2414 3173 3770 4557 4825 Size: 393 401 45 336 759 597 787 268 Cuts at: 4825 1239 Size: 1524 Fragments arranged by size: 1524 787 759 597 401 393 336 268 45 EcoRI G'AATT_C Cuts at: 2419 2797 2419 Size: 378 4732 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2227 2227 Size: 5110 FspI TGC'GCA Cuts at: 3616 3616 Size: 5110 HaeII r_GCGC'y Cuts at: 1678 4257 1678 Size: 2579 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3751 3783 3825 3857 1373 Size: 32 135 32 2179 32 42 32 2626 Fragments arranged by size: 2626 2179 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2454 2700 669 Size: 988 281 516 246 3079 Fragments arranged by size: 3079 988 516 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5110 HpaI GTT'AAC Cuts at: 2700 2700 Size: 5110 KpnI G_GTAC'C Cuts at: 2435 2435 Size: 5110 MfeI C'AATT_G Cuts at: 2709 2709 Size: 5110 MluI A'CGCG_T Cuts at: 4986 4986 Size: 5110 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4224 4408 1305 Size: 2919 184 2007 Fragments arranged by size: 2919 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3288 Fragments arranged by size: 3288 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3068 3427 3586 518 Size: 648 1902 359 159 2042 Fragments arranged by size: 2042 1902 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2526 3203 4351 4596 1606 Size: 920 677 1148 245 2120 Fragments arranged by size: 2120 1148 920 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3619 Fragments arranged by size: 3619 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5110 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5110 NotI GC'GGCC_GC Cuts at: 2557 2557 Size: 5110 NspI r_CATG'y Cuts at: 1132 1229 2468 4013 1132 Size: 97 1239 1545 2229 Fragments arranged by size: 2229 1545 1239 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5110 PciI A'CATG_T Cuts at: 4009 4009 Size: 5110 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5110 PmeI GTTT'AAAC Cuts at: 2414 2414 Size: 5110 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3179 3211 4692 4724 3179 Size: 32 1481 32 3565 Fragments arranged by size: 3565 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5110 PsiI TTA'TAA Cuts at: 2680 2680 Size: 5110 PstI C_TGCA'G Cuts at: 830 2462 830 Size: 1632 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5110 SalI G'TCGA_C Cuts at: 1936 2452 1936 Size: 516 4594 SbfI CC_TGCA'GG Cuts at: 2462 2462 Size: 5110 ScaI AGT'ACT Cuts at: 3358 5070 3358 Size: 1712 3398 SfcI C'TryA_G Cuts at: 826 1045 2458 3593 4274 4465 826 Size: 219 1413 1135 681 191 1471 Fragments arranged by size: 1471 1413 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5110 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5110 SmaI CCC'GGG Cuts at: 1872 2437 1872 Size: 565 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3214 4115 4377 820 Size: 197 926 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2901 493 Size: 2408 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5110 SphI G_CATG'C Cuts at: 1132 2468 1132 Size: 1336 3774 SspI AAT'ATT Cuts at: 5 52 3034 5 Size: 47 2982 2081 Fragments arranged by size: 2982 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5110 StyI C'CwwG_G Cuts at: 513 1065 2004 2137 2549 513 Size: 552 939 133 412 3074 Fragments arranged by size: 3074 939 552 412 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3137 3154 3307 3492 3937 325 Size: 1255 1557 17 153 185 445 1498 Fragments arranged by size: 1557 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2143 3356 5068 Size: 274 80 33 51 1443 166 1213 1712 Cuts at: 5068 96 Size: 138 Fragments arranged by size: 1712 1443 1213 274 166 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2361 2393 5070 5102 2361 Size: 32 2677 32 2369 Fragments arranged by size: 2677 2369 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5110 XbaI T'CTAG_A Cuts at: 2446 2446 Size: 5110 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5110 XmaI C'CCGG_G Cuts at: 1870 2435 1870 Size: 565 4545 XmnI GAAnn'nnTTC Cuts at: 2118 3239 2118 Size: 1121 3989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2918 276 Size: 53 83 186 936 1384 2468 Fragments arranged by size: 2468 1384 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI