(Circular) (Six-base) MAPSORT of: pFN21AB8443.seq Check: 5772 from: 1 to: 6463 pFN21AB8443 6463 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3806 1937 Size: 1869 4594 Acc65I G'GTAC_C Cuts at: 3784 3784 Size: 6463 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3333 3461 3501 3575 4526 5910 6178 Size: 653 1048 128 40 74 951 1384 268 Cuts at: 6178 1632 Size: 1917 Fragments arranged by size: 1917 1384 1048 951 653 268 128 74 40 AflII C'TTAA_G Cuts at: 820 1017 3574 820 Size: 197 2557 3709 Fragments arranged by size: 3709 2557 197 AflIII A'CryG_T Cuts at: 1203 2858 5362 6339 1203 Size: 1655 2504 977 1327 Fragments arranged by size: 2504 1655 1327 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6463 AlwNI CAG_nnn'CTG Cuts at: 2055 2333 4206 5778 2055 Size: 278 1873 1572 2740 Fragments arranged by size: 2740 1873 1572 278 ApaI G_GGCC'C Cuts at: 2299 2299 Size: 6463 ApaLI G'TGCA_C Cuts at: 4520 5676 4520 Size: 1156 5307 ApoI r'AATT_y Cuts at: 1493 2958 3772 3998 4150 6259 1493 Size: 1465 814 226 152 2109 1697 Fragments arranged by size: 2109 1697 1465 814 226 152 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3788 1870 Size: 73 67 1778 4545 Fragments arranged by size: 4545 1778 73 67 BamHI G'GATC_C Cuts at: 3541 3793 4160 3541 Size: 252 367 5844 Fragments arranged by size: 5844 367 252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2424 2819 3307 3719 3784 Size: 325 217 627 637 395 488 412 65 Cuts at: 3784 5239 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 637 627 488 412 395 325 217 65 BanII G_rGCy'C Cuts at: 1391 2189 2287 2299 3782 1391 Size: 798 98 12 1483 4072 Fragments arranged by size: 4072 1483 798 98 12 BbeI G_GCGC'C Cuts at: 3311 3311 Size: 6463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3606 928 Size: 971 1707 3785 Fragments arranged by size: 3785 1707 971 BbvCI CC'TCA_GC Cuts at: 2389 2389 Size: 6463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4654 4688 1903 Size: 34 2717 34 3678 Fragments arranged by size: 3678 2717 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3414 4355 5565 3414 Size: 941 1210 4312 Fragments arranged by size: 4312 1210 941 BclI T'GATC_A Cuts at: 1548 2755 1548 Size: 1207 5256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2282 5074 136 Size: 107 122 71 631 605 610 2792 1525 Fragments arranged by size: 2792 1525 631 610 605 122 107 71 BglII A'GATC_T Cuts at: 3622 6458 3622 Size: 2836 3627 BlpI GC'TnA_GC Cuts at: 2031 3880 2031 Size: 1849 4614 Bme1580I G_kGCm'C Cuts at: 1790 2299 3246 4524 5680 1790 Size: 509 947 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 947 509 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 4194 5152 Size: 416 53 83 399 24 660 2117 958 Cuts at: 5152 442 Size: 1753 Fragments arranged by size: 2117 1753 958 660 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2134 2152 3385 5123 1239 Size: 895 18 1233 1738 2579 Fragments arranged by size: 2579 1738 1233 895 18 Bpu10I CC'TnA_GC Cuts at: 2389 2389 Size: 6463 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 3189 3414 4588 5453 5751 2342 Size: 847 225 1174 865 298 3054 Fragments arranged by size: 3054 1174 865 847 298 225 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3257 3719 5126 882 Size: 617 1758 462 1407 2219 Fragments arranged by size: 2219 1758 1407 617 462 BsaAI yAC'GTr Cuts at: 493 1204 4254 493 Size: 711 3050 2702 Fragments arranged by size: 3050 2702 711 BsaBI GATnn'nnATC Cuts at: 6457 6457 Size: 6463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3308 4270 4652 5287 Size: 53 83 186 936 1775 962 382 635 Cuts at: 5287 275 Size: 1451 Fragments arranged by size: 1775 1451 962 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4896 5294 5568 5715 6126 4896 Size: 398 274 147 411 5233 Fragments arranged by size: 5233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6238 Fragments arranged by size: 6238 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2086 2089 2116 2276 1186 Size: 900 3 27 160 5373 Fragments arranged by size: 5373 900 160 27 3 BseYI C'CCAG_C Cuts at: 2405 2929 3338 3672 3739 5666 6162 2405 Size: 524 409 334 67 1927 496 2706 Fragments arranged by size: 2706 1927 524 496 409 334 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3158 1163 Size: 573 1422 4468 Fragments arranged by size: 4468 1422 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2189 4524 4609 5680 2189 Size: 2335 85 1071 2972 Fragments arranged by size: 2972 2335 1071 85 BsmI GAATG_Cn' Cuts at: 3974 4067 3974 Size: 93 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2189 2287 2299 3246 3782 4524 4609 Size: 399 399 98 12 947 536 742 85 Cuts at: 4609 5680 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 947 742 536 399 399 98 85 12 BspEI T'CCGG_A Cuts at: 6126 6126 Size: 6463 BspHI T'CATG_A Cuts at: 3148 3273 4352 6082 3148 Size: 125 1079 1730 3529 Fragments arranged by size: 3529 1730 1079 125 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 3818 844 Size: 2266 708 3489 Fragments arranged by size: 3489 2266 708 BsrBI CCG'CTC Cuts at: 1394 1823 4350 1394 Size: 429 2527 3507 Fragments arranged by size: 3507 2527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2601 2635 4958 5132 65 Size: 1183 1353 34 2323 174 1396 Fragments arranged by size: 2323 1396 1353 1183 174 34 BsrFI r'CCGG_y Cuts at: 1700 1810 5107 5294 1700 Size: 110 3297 187 2869 Fragments arranged by size: 3297 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6463 BstBI TT'CG_AA Cuts at: 5267 5267 Size: 6463 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstXI CCAn_nnnn'nTGG Cuts at: 2405 3256 3304 2405 Size: 851 48 5564 Fragments arranged by size: 5564 851 48 BstYI r'GATC_y Cuts at: 1972 3541 3622 3793 4160 4545 4562 6014 6100 Size: 1569 81 171 367 385 17 1452 86 Cuts at: 6100 6112 6458 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 171 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2229 2244 2259 2772 2229 Size: 15 15 513 5920 Fragments arranged by size: 5920 513 15 15 BtgI C'CryG_G Cuts at: 513 1065 2068 3584 513 Size: 552 1003 1516 3392 Fragments arranged by size: 3392 1516 1003 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2516 3979 4773 4793 2056 Size: 460 1463 794 20 3726 Fragments arranged by size: 3726 1463 794 460 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3433 5470 809 Size: 507 2117 2037 1802 Fragments arranged by size: 2117 2037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 2435 3910 Size: 54 1425 50 275 18 505 100 1475 Cuts at: 3910 4799 6428 8 Size: 889 1629 43 Fragments arranged by size: 1629 1475 1425 889 505 275 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3204 3602 4393 1353 Size: 1851 398 791 3423 Fragments arranged by size: 3423 1851 791 398 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5050 5424 5570 387 Size: 1064 138 3461 374 146 1280 Fragments arranged by size: 3461 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2187 2187 Size: 6463 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2152 2285 3333 3385 3461 3501 Size: 393 502 18 133 1048 52 76 40 Cuts at: 3501 3575 4526 5123 5910 6178 1239 Size: 74 951 597 787 268 1524 Fragments arranged by size: 1524 1048 951 787 597 502 393 268 133 76 74 52 40 18 EcoO109I rG'GnC_Cy Cuts at: 2296 2585 2296 Size: 289 6174 EcoRI G'AATT_C Cuts at: 3772 4150 3772 Size: 378 6085 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 6463 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 3311 3739 5610 1678 Size: 1633 428 1871 2531 Fragments arranged by size: 2531 1871 1633 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2315 2347 2413 Size: 32 135 32 614 32 97 32 66 Cuts at: 2413 2445 2744 2776 3614 3646 5104 5136 5178 Size: 32 299 32 838 32 1458 32 42 Cuts at: 5178 5210 1373 Size: 32 2626 Fragments arranged by size: 2626 1458 838 614 299 135 97 66 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3807 4053 669 Size: 988 281 1869 246 3079 Fragments arranged by size: 3079 1869 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 4053 4053 Size: 6463 KasI G'GCGC_C Cuts at: 3307 3307 Size: 6463 KpnI G_GTAC'C Cuts at: 3788 3788 Size: 6463 MfeI C'AATT_G Cuts at: 4062 4062 Size: 6463 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1998 5577 5761 1305 Size: 693 3579 184 2007 Fragments arranged by size: 3579 2007 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2337 10 Size: 54 1425 343 505 4136 Fragments arranged by size: 4136 1425 505 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4421 4780 4939 518 Size: 648 3255 359 159 2042 Fragments arranged by size: 3255 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3743 3879 4556 5704 5949 1606 Size: 2137 136 677 1148 245 2120 Fragments arranged by size: 2137 2120 1148 677 245 136 NarI GG'CG_CC Cuts at: 3308 3308 Size: 6463 NcoI C'CATG_G Cuts at: 513 1065 2068 513 Size: 552 1003 4908 Fragments arranged by size: 4908 1003 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 2862 3821 5366 1132 Size: 97 1633 959 1545 2229 Fragments arranged by size: 2229 1633 1545 959 97 PasI CC'CwG_GG Cuts at: 1348 2110 2280 1348 Size: 762 170 5531 Fragments arranged by size: 5531 762 170 PciI A'CATG_T Cuts at: 2858 5362 2858 Size: 2504 3959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6463 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4532 4564 6045 6077 4532 Size: 32 1481 32 4918 Fragments arranged by size: 4918 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2585 2585 Size: 6463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 3224 4033 3224 Size: 809 5654 PspOMI G'GGCC_C Cuts at: 2295 2295 Size: 6463 PstI C_TGCA'G Cuts at: 830 3815 830 Size: 2985 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 PvuII CAG'CTG Cuts at: 3743 3743 Size: 6463 SacI G_AGCT'C Cuts at: 2189 2189 Size: 6463 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 4711 6423 4711 Size: 1712 4751 SfcI C'TryA_G Cuts at: 826 1045 2748 3811 4946 5627 5818 826 Size: 219 1703 1063 1135 681 191 1471 Fragments arranged by size: 1703 1471 1135 1063 681 219 191 SfoI GGC'GCC Cuts at: 3309 3309 Size: 6463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3790 1872 Size: 1918 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2321 3204 3429 3574 4567 5468 Size: 197 926 378 883 225 145 993 901 Cuts at: 5468 5730 820 Size: 262 1553 Fragments arranged by size: 1553 993 926 901 883 378 262 225 197 145 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 3683 152 Size: 3531 2932 SphI G_CATG'C Cuts at: 1132 3821 1132 Size: 2689 3774 SspI AAT'ATT Cuts at: 5 52 2501 4387 5 Size: 47 2449 1886 2081 Fragments arranged by size: 2449 2081 1886 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6463 StyI C'CwwG_G Cuts at: 513 1065 2068 2089 3902 513 Size: 552 1003 21 1813 3074 Fragments arranged by size: 3074 1813 1003 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4490 4507 4660 4845 5290 325 Size: 1255 2910 17 153 185 445 1498 Fragments arranged by size: 2910 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4709 6421 96 Size: 274 80 33 51 1443 2732 1712 138 Fragments arranged by size: 2732 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2401 2433 6423 6455 2401 Size: 32 3990 32 2409 Fragments arranged by size: 3990 2409 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2200 2329 3518 1316 Size: 884 129 1189 4261 Fragments arranged by size: 4261 1189 884 129 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6463 XmaI C'CCGG_G Cuts at: 1870 3788 1870 Size: 1918 4545 XmnI GAAnn'nnTTC Cuts at: 4592 4592 Size: 6463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI