Restriction map for pFN21AB8443
 (Linear) (Six-base) MAPSORT of: KIBB8443.seq  Check: 1734  from: 1  to: 1758



KIBB8443.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1328    1456    1496    1570    1758 
   Size:        280    1048     128      40      74     188

  Fragments arranged by size: 

                1048     280     188     128      74      40
 
AflII C'TTAA_G

Cuts at:      0    1569    1758 
   Size:       1569     189
 
AflIII A'CryG_T

Cuts at:      0     853    1758 
   Size:        853     905
 
AlwNI CAG_nnn'CTG

Cuts at:      0      50     328    1758 
   Size:         50     278    1430

  Fragments arranged by size: 

                1430     278      50
 
ApaI G_GGCC'C

Cuts at:      0     294    1758 
   Size:        294    1464
 
ApoI r'AATT_y

Cuts at:      0     953    1758 
   Size:        953     805
 
AvaI C'yCGr_G

Cuts at:      0       5    1758 
   Size:          5    1753
 
BamHI G'GATC_C

Cuts at:      0    1536    1758 
   Size:       1536     222
 
BanI G'GyrC_C

Cuts at:      0     419     814    1302    1714    1758 
   Size:        419     395     488     412      44

  Fragments arranged by size: 

                 488     419     412     395      44
 
BanII G_rGCy'C

Cuts at:      0     184     282     294    1758 
   Size:        184      98      12    1464

  Fragments arranged by size: 

                1464     184      98      12
 
BbeI G_GCGC'C

Cuts at:      0    1306    1758 
   Size:       1306     452
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1601    1758 
   Size:       1601     157
 
BbvCI CC'TCA_GC

Cuts at:      0     384    1758 
   Size:        384    1374
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1409    1758 
   Size:       1409     349
 
BclI T'GATC_A

Cuts at:      0     750    1758 
   Size:        750    1008
 
BglI GCCn_nnn'nGGC

Cuts at:      0     277    1758 
   Size:        277    1481
 
BglII A'GATC_T

Cuts at:      0    1617    1758 
   Size:       1617     141
 
BlpI GC'TnA_GC

Cuts at:      0      26    1758 
   Size:         26    1732
 
Bme1580I G_kGCm'C

Cuts at:      0     294    1241    1758 
   Size:        294     947     517

  Fragments arranged by size: 

                 947     517     294
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      72    1758 
   Size:         72    1686
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     147    1380    1758 
   Size:        129      18    1233     378

  Fragments arranged by size: 

                1233     378     129      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     384    1758 
   Size:        384    1374
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337    1184    1409    1758 
   Size:        337     847     225     349

  Fragments arranged by size: 

                 847     349     337     225
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1252    1714    1758 
   Size:       1252     462      44

  Fragments arranged by size: 

                1252     462      44
 
BsaHI Gr'CG_yC

Cuts at:      0    1303    1758 
   Size:       1303     455
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      81      84     111     271    1758 
   Size:         81       3      27     160    1487

  Fragments arranged by size: 

                1487     160      81      27       3
 
BseYI C'CCAG_C

Cuts at:      0     400     924    1333    1667    1734    1758 
   Size:        400     524     409     334      67      24

  Fragments arranged by size: 

                 524     409     400     334      67      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1153    1758 
   Size:       1153     605
 
BsiHKAI G_wGCw'C

Cuts at:      0     184    1758 
   Size:        184    1574
 
Bsp1286I G_dGCh'C

Cuts at:      0     184     282     294    1241    1758 
   Size:        184      98      12     947     517

  Fragments arranged by size: 

                 947     517     184      98      12
 
BspHI T'CATG_A

Cuts at:      0    1143    1268    1758 
   Size:       1143     125     490

  Fragments arranged by size: 

                1143     490     125
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1105    1758 
   Size:       1105     653
 
BsrDI GCAATG_nn'

Cuts at:      0     596     630    1758 
   Size:        596      34    1128

  Fragments arranged by size: 

                1128     596      34
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     400    1251    1299    1758 
   Size:        400     851      48     459

  Fragments arranged by size: 

                 851     459     400      48
 
BstYI r'GATC_y

Cuts at:      0    1536    1617    1758 
   Size:       1536      81     141

  Fragments arranged by size: 

                1536     141      81
 
Bsu36I CC'TnA_GG

Cuts at:      0     224     239     254     767    1758 
   Size:        224      15      15     513     991

  Fragments arranged by size: 

                 991     513     224      15      15
 
BtgI C'CryG_G

Cuts at:      0      63    1579    1758 
   Size:         63    1516     179

  Fragments arranged by size: 

                1516     179      63
 
BtsI GCAGTG_nn'

Cuts at:      0      51     511    1758 
   Size:         51     460    1247

  Fragments arranged by size: 

                1247     460      51
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1428    1758 
   Size:       1428     330
 
EaeI y'GGCC_r

Cuts at:      0     330     430    1758 
   Size:        330     100    1328

  Fragments arranged by size: 

                1328     330     100
 
EarI CTCTTCn'nnn_

Cuts at:      0    1199    1597    1758 
   Size:       1199     398     161

  Fragments arranged by size: 

                1199     398     161
 
EcoICRI GAG'CTC

Cuts at:      0     182    1758 
   Size:        182    1576
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     147     280    1328    1380    1456    1496    1570 
   Size:        129      18     133    1048      52      76      40      74

Cuts at:   1570    1758 
   Size:        188

  Fragments arranged by size: 

                1048     188     133     129      76      74      52      40
                  18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     291     580    1758 
   Size:        291     289    1178

  Fragments arranged by size: 

                1178     291     289
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     573    1758 
   Size:        573    1185
 
HaeII r_GCGC'y

Cuts at:      0    1306    1734    1758 
   Size:       1306     428      24

  Fragments arranged by size: 

                1306     428      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     310     342     408     440     739     771 
   Size:        181      32      97      32      66      32     299      32

Cuts at:    771    1609    1641    1758 
   Size:        838      32     117

  Fragments arranged by size: 

                 838     299     181     117      97      66      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1302    1758 
   Size:       1302     456
 
MscI TGG'CCA

Cuts at:      0     332    1758 
   Size:        332    1426
 
MspA1I CmG'CkG

Cuts at:      0    1738    1758 
   Size:       1738      20
 
NarI GG'CG_CC

Cuts at:      0    1303    1758 
   Size:       1303     455
 
NcoI C'CATG_G

Cuts at:      0      63    1758 
   Size:         63    1695
 
NspI r_CATG'y

Cuts at:      0     857    1758 
   Size:        857     901
 
PasI CC'CwG_GG

Cuts at:      0     105     275    1758 
   Size:        105     170    1483

  Fragments arranged by size: 

                1483     170     105
 
PciI A'CATG_T

Cuts at:      0     853    1758 
   Size:        853     905
 
PpuMI rG'GwC_Cy

Cuts at:      0     580    1758 
   Size:        580    1178
 
PsiI TTA'TAA

Cuts at:      0    1219    1758 
   Size:       1219     539
 
PspOMI G'GGCC_C

Cuts at:      0     290    1758 
   Size:        290    1468
 
PvuII CAG'CTG

Cuts at:      0    1738    1758 
   Size:       1738      20
 
SacI G_AGCT'C

Cuts at:      0     184    1758 
   Size:        184    1574
 
SfcI C'TryA_G

Cuts at:      0     743    1758 
   Size:        743    1015
 
SfoI GGC'GCC

Cuts at:      0    1304    1758 
   Size:       1304     454
 
SmlI C'TyrA_G

Cuts at:      0     316    1199    1424    1569    1758 
   Size:        316     883     225     145     189

  Fragments arranged by size: 

                 883     316     225     189     145
 
SpeI A'CTAG_T

Cuts at:      0    1678    1758 
   Size:       1678      80
 
SspI AAT'ATT

Cuts at:      0     496    1758 
   Size:        496    1262
 
StyI C'CwwG_G

Cuts at:      0      63      84    1758 
   Size:         63      21    1674

  Fragments arranged by size: 

                1674      63      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     396     428    1758 
   Size:        396      32    1330

  Fragments arranged by size: 

                1330     396      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     195     324    1513    1758 
   Size:        195     129    1189     245

  Fragments arranged by size: 

                1189     245     195     129
 

 Enzymes that do cut:

     AcuI    AflII   AflIII    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KasI
     MscI   MspA1I     NarI     NcoI     NspI     PasI     PciI    PpuMI
     PsiI   PspOMI    PvuII     SacI     SfcI     SfoI     SmlI     SpeI
     SspI     StyI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BcgI    BfrBI    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI
    TaqII     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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