Restriction map for pFN21AB7323
 (Circular) (Six-base) MAPSORT of: pFN21AB7323.seq  Check: 775  from: 1  to: 10567



pFN21AB7323  10567 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2689    3092    3330    5300    5473    2689 
   Size:        403     238    1970     173    7783

  Fragments arranged by size: 

                7783    1970     403     238     173
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    8377     278 
   Size:         53      83     186     936    6841    2468

  Fragments arranged by size: 

                6841    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    7910    1937 
   Size:       5973    4594
 
Acc65I G'GTAC_C

Cuts at:   7888    7888 
   Size:      10567
 
AclI AA'CG_TT

Cuts at:   1563    8694    9067    1563 
   Size:       7131     373    3063

  Fragments arranged by size: 

                7131    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2479    3419    4625    5055    5123    5380    6224    7515 
   Size:        847     940    1206     430      68     257     844    1291

Cuts at:   7515    8630   10014   10282    1632 
   Size:       1115    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1291    1206    1115     940     847     844
                 430     268     257      68
 
AfeI AGC'GCT

Cuts at:   3746    3746 
   Size:      10567
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   10370
 
AflIII A'CryG_T

Cuts at:   1203    4809    9466   10443    1203 
   Size:       3606    4657     977    1327

  Fragments arranged by size: 

                4657    3606    1327     977
 
AgeI A'CCGG_T

Cuts at:   9398    9398 
   Size:      10567
 
AhdI GACnn_n'nnGTC

Cuts at:   2077    9296    2077 
   Size:       7219    3348
 
AleI CACnn'nnGTG

Cuts at:   1166    5483    6429    6653    1166 
   Size:       4317     946     224    5080

  Fragments arranged by size: 

                5080    4317     946     224
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    4217    5519    1184 
   Size:       3033    1302    6232

  Fragments arranged by size: 

                6232    3033    1302
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2435    2467    4373    4405    4556    4588    4646    4678    2435 
   Size:         32    1906      32     151      32      58      32    8324

  Fragments arranged by size: 

                8324    1906     151      58      32      32      32      32
            
 
AlwNI CAG_nnn'CTG

Cuts at:   5569    6772    7528    8310    9882    5569 
   Size:       1203     756     782    1572    6254

  Fragments arranged by size: 

                6254    1572    1203     782     756
 
ApaI G_GGCC'C

Cuts at:   2015    4153    4257    5278    6606    7791    7805    7851    2015 
   Size:       2138     104    1021    1328    1185      14      46    4731

  Fragments arranged by size: 

                4731    2138    1328    1185    1021     104      46      14
            
 
ApaLI G'TGCA_C

Cuts at:   3827    3868    8624    9780    3827 
   Size:         41    4756    1156    4614

  Fragments arranged by size: 

                4756    4614    1156      41
 
ApoI r'AATT_y

Cuts at:   1493    4457    7103    7876    8102    8254   10363    1493 
   Size:       2964    2646     773     226     152    2109    1697

  Fragments arranged by size: 

                2964    2646    2109    1697     773     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    9121     160 
   Size:        872    8089    1606

  Fragments arranged by size: 

                8089    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10567
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2404    2872    3088    3137    3347    5270    5304 
   Size:         73     461     468     216      49     210    1923      34

Cuts at:   5304    5454    6243    6477    6605    6746    6855    7892    1870 
   Size:        150     789     234     128     141     109    1037    4545

  Fragments arranged by size: 

                4545    1923    1037     789     468     461     234     216
                 210     150     141     128     109      73      49      34
            
 
BamHI G'GATC_C

Cuts at:   3177    7897    8264    3177 
   Size:       4720     367    5480

  Fragments arranged by size: 

                5480    4720     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2021    2570    2701    4318    5124 
   Size:        325     217     627     234     549     131    1617     806

Cuts at:   5124    5979    6423    7212    7218    7888    9343     618 
   Size:        855     444     789       6     670    1455    1842

  Fragments arranged by size: 

                1842    1617    1455     855     806     789     670     627
                 549     444     325     234     217     131       6
 
BanII G_rGCy'C

Cuts at:   1391    2015    2136    2397    2826    3444    3489    4153    4257 
   Size:        624     121     261     429     618      45     664     104

Cuts at:   4257    4488    4517    4842    5278    5864    6099    6606    6836 
   Size:        231      29     325     436     586     235     507     230

Cuts at:   6836    6856    7035    7317    7791    7805    7851    7886    1391 
   Size:         20     179     282     474      14      46      35    4072

  Fragments arranged by size: 

                4072     664     624     618     586     507     474     436
                 429     325     282     261     235     231     230     179
                 121     104      46      45      35      29      20      14
            
 
BbeI G_GCGC'C

Cuts at:   4322    7222    4322 
   Size:       2900    7667
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3693    3963    4752    5061     928 
   Size:        971    1794     270     789     309    6434

  Fragments arranged by size: 

                6434    1794     971     789     309     270
 
BbvCI CC'TCA_GC

Cuts at:   4001    7020    4001 
   Size:       3019    7548
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2933    2967    8758    8792    1903 
   Size:         34      50      34     912      34    5791      34    3678

  Fragments arranged by size: 

                5791    3678     912      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   7195    8459    9669    7195 
   Size:       1264    1210    8093

  Fragments arranged by size: 

                8093    1264    1210
 
BclI T'GATC_A

Cuts at:   1548    3837    1548 
   Size:       2289    8278
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2019    3976    5018 
   Size:        107     122      71     631     605     347    1957    1042

Cuts at:   5018    5273    6169    7490    7846    9178     136 
   Size:        255     896    1321     356    1332    1525

  Fragments arranged by size: 

                1957    1525    1332    1321    1042     896     631     605
                 356     347     255     122     107      71
 
BglII A'GATC_T

Cuts at:  10562   10562 
   Size:      10567
 
BlpI GC'TnA_GC

Cuts at:   4859    6962    7607    7984    4859 
   Size:       2103     645     377    7442

  Fragments arranged by size: 

                7442    2103     645     377
 
Bme1580I G_kGCm'C

Cuts at:   1790    2015    2024    2706    3831    3872    4153    4160    4257 
   Size:        225       9     682    1125      41     281       7      97

Cuts at:   4257    5127    5278    6477    6606    6660    6754    7791    7805 
   Size:        870     151    1199     129      54      94    1037      14

Cuts at:   7805    7851    8628    9784    1790 
   Size:         46     777    1156    2573

  Fragments arranged by size: 

                2573    1199    1156    1125    1037     870     777     682
                 281     225     151     129      97      94      54      46
                  41      14       9       7
 
BmgBI CAC'GTC

Cuts at:   1318    2440    3481    4810    5617    5749    7627    1318 
   Size:       1122    1041    1329     807     132    1878    4258

  Fragments arranged by size: 

                4258    1878    1329    1122    1041     807     132
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2009    3937    4303 
   Size:        416      53      83     399      24     592    1928     366

Cuts at:   4303    5971    8298    9256     442 
   Size:       1668    2327     958    1753

  Fragments arranged by size: 

                2327    1928    1753    1668     958     592     416     399
                 366      83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    2684    1056 
   Size:       1628    8939
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4261    4381    6505    6649    4261 
   Size:        120    2124     144    8179

  Fragments arranged by size: 

                8179    2124     144     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2775    3146    3448    3533    3617    4139    4671    4673 
   Size:       1536     371     302      85      84     522     532       2

Cuts at:   4673    4727    4739    5192    5204    6475    6977    7370    7412 
   Size:         54      12     453      12    1271     502     393      42

Cuts at:   7412    7616    9227    1239 
   Size:        204    1611    2579

  Fragments arranged by size: 

                2579    1611    1536    1271     532     522     502     453
                 393     371     302     204      85      84      54      42
                  12      12       2
 
Bpu10I CC'TnA_GC

Cuts at:   3504    4001    4790    5858    7020    7703    3504 
   Size:        497     789    1068    1162     683    6368

  Fragments arranged by size: 

                6368    1162    1068     789     683     497
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2156    2297    2810    3899    3968    4544    4784    5567    8692 
   Size:        141     513    1089      69     576     240     783    3125

Cuts at:   8692    9557    9855    2156 
   Size:        865     298    2868

  Fragments arranged by size: 

                3125    2868    1089     865     783     576     513     298
                 240     141      69
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    6449    9230     882 
   Size:        617    4950    2781    2219

  Fragments arranged by size: 

                4950    2781    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4333    4465    5884    6502    8358     493 
   Size:        711    3129     132    1419     618    1856    2702

  Fragments arranged by size: 

                3129    2702    1856    1419     711     618     132
 
BsaBI GATnn'nnATC

Cuts at:  10561   10561 
   Size:      10567
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3300    4319    6339    7219 
   Size:         53      83     186     936    1767    1019    2020     880

Cuts at:   7219    8374    8756    9391     275 
   Size:       1155     382     635    1451

  Fragments arranged by size: 

                2020    1767    1451    1155    1019     936     880     635
                 382     186      83      53
 
BsaWI w'CCGG_w

Cuts at:   5805    7422    9000    9398    9672    9819   10230    5805 
   Size:       1617    1578     398     274     147     411    6142

  Fragments arranged by size: 

                6142    1617    1578     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2384    2414    2915    2945    4070 
   Size:         30     165      30     981      30     501      30    1125

Cuts at:   4070    4100    4370    4400    4646    4676    5846    5876    6050 
   Size:         30     270      30     246      30    1170      30     174

Cuts at:   6050    6080    7385    7415    7806    7836    1178 
   Size:         30    1305      30     391      30    3909

  Fragments arranged by size: 

                3909    1305    1170    1125     981     501     391     270
                 246     174     165      30      30      30      30      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2042    2045    2069    2474    2860    2909    2992    3227 
   Size:        856       3      24     405     386      49      83     235

Cuts at:   3227    3230    3245    3248    3251    3301    3755    4661    4670 
   Size:          3      15       3       3      50     454     906       9

Cuts at:   4670    4736    5285    5483    5909    5924    5954    6625    6875 
   Size:         66     549     198     426      15      30     671     250

Cuts at:   6875    6887    6932    7037    7184    7427    7613    7750    1186 
   Size:         12      45     105     147     243     186     137    4003

  Fragments arranged by size: 

                4003     906     856     671     549     454     426     405
                 386     250     243     235     198     186     147     137
                 105      83      66      50      49      45      30      24
                  15      15      12       9       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:   2706    3488    3949    4774    5277    6268    6318    6691    6717 
   Size:        782     461     825     503     991      50     373      26

Cuts at:   6717    6721    6835    7547    9770   10266    2706 
   Size:          4     114     712    2223     496    3007

  Fragments arranged by size: 

                3007    2223     991     825     782     712     503     496
                 461     373     114      50      26       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    4088    4223    4469    6471    7559    1163 
   Size:        573    2352     135     246    2002    1088    4171

  Fragments arranged by size: 

                4171    2352    2002    1088     573     246     135
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2820    8017    8778    9806    1540 
   Size:        275     186     819    5197     761    1028    2301

  Fragments arranged by size: 

                5197    2301    1028     819     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3831    3872    4488    4842    5136    5864    6099    8628    8713 
   Size:         41     616     354     294     728     235    2529      85

Cuts at:   8713    9784    3831 
   Size:       1071    4614

  Fragments arranged by size: 

                4614    2529    1071     728     616     354     294     235
                  85      41
 
BsmI GAATG_Cn'

Cuts at:   2951    8078    8171    2951 
   Size:       5127      93    5347

  Fragments arranged by size: 

                5347    5127      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2651    6869    2651 
   Size:       4218    6349
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2015    2024    2136    2397    2706    2826    3444 
   Size:        399     225       9     112     261     309     120     618

Cuts at:   3444    3489    3831    3872    4153    4160    4257    4488    4517 
   Size:         45     342      41     281       7      97     231      29

Cuts at:   4517    4842    5127    5136    5278    5864    6099    6477    6606 
   Size:        325     285       9     142     586     235     378     129

Cuts at:   6606    6660    6754    6836    6856    7035    7317    7791    7805 
   Size:         54      94      82      20     179     282     474      14

Cuts at:   7805    7851    7886    8628    8713    9784    1391 
   Size:         46      35     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     618     586     474     399     378
                 342     325     309     285     282     281     261     235
                 231     225     179     142     129     120     112      97
                  94      85      82      54      46      45      41      35
                  29      20      14       9       9       7
 
BspEI T'CCGG_A

Cuts at:   7422   10230    7422 
   Size:       2808    7759
 
BspHI T'CATG_A

Cuts at:   4959    8456   10186    4959 
   Size:       3497    1730    5340

  Fragments arranged by size: 

                5340    3497    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2689    3092    3330    3861    4779    5300    5473    6037 
   Size:       1845     403     238     531     918     521     173     564

Cuts at:   6037    7368    7922     844 
   Size:       1331     554    3489

  Fragments arranged by size: 

                3489    1845    1331     918     564     554     531     521
                 403     238     173
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4365    5209    5920    6865    7174    8454    1394 
   Size:        429    2542     844     711     945     309    1280    3507

  Fragments arranged by size: 

                3507    2542    1280     945     844     711     429     309
            
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2224    2517    3325    3568    9062    9236      65 
   Size:       1183     976     293     808     243    5494     174    1396

  Fragments arranged by size: 

                5494    1396    1183     976     808     293     243     174
            
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4825    5647    7215    9211    9398    1700 
   Size:        110    3015     822    1568    1996     187    2869

  Fragments arranged by size: 

                3015    2869    1996    1568     822     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    7770      96 
   Size:       7674    2893
 
BssHII G'CGCG_C

Cuts at:   1925    4269    1925 
   Size:       2344    8223
 
BssSI C'ACGA_G

Cuts at:   6827    8627    9639    6827 
   Size:       1800    1012    7755

  Fragments arranged by size: 

                7755    1800    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    4771    5131    6502     794 
   Size:       3977     360    1371    4859

  Fragments arranged by size: 

                4859    3977    1371     360
 
BstBI TT'CG_AA

Cuts at:   9371    9371 
   Size:      10567
 
BstEII G'GTnAC_C

Cuts at:   1184    2662    4336    1184 
   Size:       1478    1674    7415

  Fragments arranged by size: 

                7415    1674    1478
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3059    5161    5800    3059 
   Size:       2102     639    7826

  Fragments arranged by size: 

                7826    2102     639
 
BstYI r'GATC_y

Cuts at:   1972    2245    2538    3177    3351    6942    7897    8264    8649 
   Size:        273     293     639     174    3591     955     367     385

Cuts at:   8649    8666   10118   10204   10216   10562    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                3591    1977    1452     955     639     385     367     346
                 293     273     174      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2507    6597    2507 
   Size:       4090    6477
 
BtgI C'CryG_G

Cuts at:    513    1065    2039    2619    4050    4073    5013    5334    7292 
   Size:        552     974     580    1431      23     940     321    1958

Cuts at:   7292    7470     513 
   Size:        178    3610

  Fragments arranged by size: 

                3610    1958    1431     974     940     580     552     321
                 178      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    7108     487 
   Size:        683      56    5882    3946

  Fragments arranged by size: 

                5882    3946     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2281    4100    5767    8083    8877    8897    2281 
   Size:       1819    1667    2316     794      20    3951

  Fragments arranged by size: 

                3951    2316    1819    1667     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    3882    1554 
   Size:       2328    8239
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3308    3343     529 
   Size:         35    2744      35    7753

  Fragments arranged by size: 

                7753    2744      35      35
 
DraI TTT'AAA

Cuts at:   7871    7871 
   Size:      10567
 
DraIII CAC_nnn'GTG

Cuts at:   3613    5131    5518    7158    3613 
   Size:       1518     387    1640    7022

  Fragments arranged by size: 

                7022    1640    1518     387
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4052    9574     809 
   Size:        507    2736    5522    1802

  Fragments arranged by size: 

                5522    2736    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2418    2817    4173 
   Size:         54    1425      50     275      18     588     399    1356

Cuts at:   4173    4827    5331    5416    5487    5694    5784    6866    7668 
   Size:        654     504      85      71     207      90    1082     802

Cuts at:   7668    8014    8903   10532       8 
   Size:        346     889    1629      43

  Fragments arranged by size: 

                1629    1425    1356    1082     889     802     654     588
                 504     399     346     275     207      90      85      71
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2817    8014    1537 
   Size:        275    1005    5197    4090

  Fragments arranged by size: 

                5197    4090    1005     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2235    2247    2963    2987    3204    3698    5003    5609 
   Size:        882      12     716      24     217     494    1305     606

Cuts at:   5609    6150    6200    6374    6419    8497    1353 
   Size:        541      50     174      45    2078    3423

  Fragments arranged by size: 

                3423    2078    1305     882     716     606     541     494
                 217     174      50      45      24      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5354    6458    9154    9528    9674     387 
   Size:       1064     138    3765    1104    2696     374     146    1280

  Fragments arranged by size: 

                3765    2696    1280    1104    1064     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   4486    4840    5862    6097    4486 
   Size:        354    1022     235    8956

  Fragments arranged by size: 

                8956    1022     354     235
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2479    2775    3146    3419    3448    3533    3617 
   Size:        393     847     296     371     273      29      85      84

Cuts at:   3617    4139    4625    4671    4673    4727    4739    5055    5123 
   Size:        522     486      46       2      54      12     316      68

Cuts at:   5123    5192    5204    5380    6224    6475    6977    7370    7412 
   Size:         69      12     176     844     251     502     393      42

Cuts at:   7412    7515    7616    8630    9227   10014   10282    1239 
   Size:        103     101    1014     597     787     268    1524

  Fragments arranged by size: 

                1524    1014     847     844     787     597     522     502
                 486     393     393     371     316     296     273     268
                 251     176     103     101      85      84      69      68
                  54      46      42      29      12      12       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3088    3917    4232    5171    5240    6023    7532    7577    3088 
   Size:        829     315     939      69     783    1509      45    6078

  Fragments arranged by size: 

                6078    1509     939     829     783     315      69      45
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2011    2428    2750    3093    3473    3718    4123    4253    4254 
   Size:        417     322     343     380     245     405     130       1

Cuts at:   4254    4632    5020    5233    5274    6004    6367    6484    6602 
   Size:        378     388     213      41     730     363     117     118

Cuts at:   6602    6784    6886    7453    7582    7729    7801    7802    7847 
   Size:        182     102     567     129     147      72       1      45

Cuts at:   7847    7848    2011 
   Size:          1    4730

  Fragments arranged by size: 

                4730     730     567     417     405     388     380     378
                 363     343     322     245     213     182     147     130
                 129     118     117     102      72      45      41       1
                   1       1
 
EcoRI G'AATT_C

Cuts at:   7876    8254    7876 
   Size:        378   10189
 
EcoRV GAT'ATC

Cuts at:   7864    7864 
   Size:      10567
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2851    2851 
   Size:      10567
 
FspI TGC'GCA

Cuts at:   9073    9073 
   Size:      10567
 
HaeII r_GCGC'y

Cuts at:   1678    2595    2916    3748    4322    7222    7752    9714    1678 
   Size:        917     321     832     574    2900     530    1962    2531

  Fragments arranged by size: 

                2900    2531    1962     917     832     574     530     321
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2063    2095    2213    2245    2384 
   Size:         32     135      32     491      32     118      32     139

Cuts at:   2384    2416    3659    3691    5039    5071    5657    5689    6083 
   Size:         32    1243      32    1348      32     586      32     394

Cuts at:   6083    6095    6115    6127    7806    7838    9208    9240    9282 
   Size:         12      20      12    1679      32    1370      32      42

Cuts at:   9282    9314    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1679    1370    1348    1243     586     491     394
                 139     135     118      42      32      32      32      32
                  32      32      32      32      32      32      32      20
                  12      12
 
HincII GTy'rAC

Cuts at:    669    1657    1938    6052    6565    7825    7911    8157     669 
   Size:        988     281    4114     513    1260      86     246    3079

  Fragments arranged by size: 

                4114    3079    1260     988     513     281     246      86
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:      10567
 
HpaI GTT'AAC

Cuts at:   8157    8157 
   Size:      10567
 
KasI G'GCGC_C

Cuts at:   4318    7218    4318 
   Size:       2900    7667
 
KpnI G_GTAC'C

Cuts at:   7892    7892 
   Size:      10567
 
MfeI C'AATT_G

Cuts at:   8166    8166 
   Size:      10567
 
MluI A'CGCG_T

Cuts at:  10443   10443 
   Size:      10567
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2241    2880    7668    9681    9865    1305 
   Size:        936     639    4788    2013     184    2007

  Fragments arranged by size: 

                4788    2013    2007     936     639     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    5333    5489      10 
   Size:         54    1425     343    3501     156    5088

  Fragments arranged by size: 

                5088    3501    1425     343     156      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3614    4961    5483    5985    6263    6429    6653 
   Size:        648    2448    1347     522     502     278     166     224

Cuts at:   6653    8525    8884    9043     518 
   Size:       1872     359     159    2042

  Fragments arranged by size: 

                2448    2042    1872    1347     648     522     502     359
                 278     224     166     159
 
MspA1I CmG'CkG

Cuts at:   1606    2041    2621    2630    3343    3415    3595    4117    6202 
   Size:        435     580       9     713      72     180     522    2085

Cuts at:   6202    6289    6691    6721    6907    7269    7594    7983    8660 
   Size:         87     402      30     186     362     325     389     677

Cuts at:   8660    9808   10053    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    2085    1148     713     677     580     522     435
                 402     389     362     325     245     186     180      87
                  72      30       9
 
NaeI GCC'GGC

Cuts at:   4827    4827 
   Size:      10567
 
NarI GG'CG_CC

Cuts at:   4319    7219    4319 
   Size:       2900    7667
 
NcoI C'CATG_G

Cuts at:    513    1065    4073    7470     513 
   Size:        552    3008    3397    3610

  Fragments arranged by size: 

                3610    3397    3008     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:      10567
 
NgoMIV G'CCGG_C

Cuts at:   4825    4825 
   Size:      10567
 
NheI G'CTAG_C

Cuts at:   1052    2680    1052 
   Size:       1628    8939
 
NotI GC'GGCC_GC

Cuts at:   8014    8014 
   Size:      10567
 
NspI r_CATG'y

Cuts at:   1132    1229    4789    4825    6328    6563    7925    9470    1132 
   Size:         97    3560      36    1503     235    1362    1545    2229

  Fragments arranged by size: 

                3560    2229    1545    1503    1362     235      97      36
            
 
PasI CC'CwG_GG

Cuts at:   1348    2959    4153    7843    1348 
   Size:       1611    1194    3690    4072

  Fragments arranged by size: 

                4072    3690    1611    1194
 
PciI A'CATG_T

Cuts at:   9466    9466 
   Size:      10567
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    4524    5518    6358    1108 
   Size:       3416     994     840    5317

  Fragments arranged by size: 

                5317    3416     994     840
 
PfoI T'CCnGG_A

Cuts at:   4716    5169    5181    6191    6945    4716 
   Size:        453      12    1010     754    8338

  Fragments arranged by size: 

                8338    1010     754     453      12
 
PmeI GTTT'AAAC

Cuts at:   7871    7871 
   Size:      10567
 
PmlI CAC'GTG

Cuts at:   4465    6502    4465 
   Size:       2037    8530
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3518    3550    4646    4678    8636    8668   10149   10181    3518 
   Size:         32    1096      32    3958      32    1481      32    3904

  Fragments arranged by size: 

                3958    3904    1481    1096      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:   3718    4632    6004    6367    7453    7582    7729    3718 
   Size:        914    1372     363    1086     129     147    6556

  Fragments arranged by size: 

                6556    1372    1086     914     363     147     129
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:      10567
 
PsiI TTA'TAA

Cuts at:   8137    8137 
   Size:      10567
 
PspOMI G'GGCC_C

Cuts at:   2011    4149    4253    5274    6602    7787    7801    7847    2011 
   Size:       2138     104    1021    1328    1185      14      46    4731

  Fragments arranged by size: 

                4731    2138    1328    1185    1021     104      46      14
            
 
PspXI vC'TCGA_Gb

Cuts at:   2872    2872 
   Size:      10567
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   4871    4903    4871 
   Size:         32   10535
 
PstI C_TGCA'G

Cuts at:    830    2956    7683    7919     830 
   Size:       2126    4727     236    3478

  Fragments arranged by size: 

                4727    3478    2126     236
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      10567
 
PvuII CAG'CTG

Cuts at:   3595    4117    6202    6721    6907    7594    3595 
   Size:        522    2085     519     186     687    6568

  Fragments arranged by size: 

                6568    2085     687     522     519     186
 
SacI G_AGCT'C

Cuts at:   4488    4842    5864    6099    4488 
   Size:        354    1022     235    8956

  Fragments arranged by size: 

                8956    1022     354     235
 
SacII CC_GC'GG

Cuts at:   2042    2622    2042 
   Size:        580    9987
 
SalI G'TCGA_C

Cuts at:   1936    7909    1936 
   Size:       5973    4594
 
SanDI GG'GwC_CC

Cuts at:   4632    7729    4632 
   Size:       3097    7470
 
SapI GCTCTTCn'nnn_

Cuts at:   2963    6374    2963 
   Size:       3411    7156
 
SbfI CC_TGCA'GG

Cuts at:   7919    7919 
   Size:      10567
 
ScaI AGT'ACT

Cuts at:   4880    8815   10527    4880 
   Size:       3935    1712    4920

  Fragments arranged by size: 

                4920    3935    1712
 
SexAI A'CCwGG_T

Cuts at:   4260    5514    4260 
   Size:       1254    9313
 
SfcI C'TryA_G

Cuts at:    826    1045    2652    2952    3590    5090    5820    5837    7679 
   Size:        219    1607     300     638    1500     730      17    1842

Cuts at:   7679    7915    9050    9731    9922     826 
   Size:        236    1135     681     191    1471

  Fragments arranged by size: 

                1842    1607    1500    1471    1135     730     681     638
                 300     236     219     191      17
 
SfoI GGC'GCC

Cuts at:   4320    7220    4320 
   Size:       2900    7667
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10567
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    7215    1700 
   Size:       5515    5052
 
SmaI CCC'GGG

Cuts at:   1872    5272    5306    6479    6748    7894    1872 
   Size:       3400      34    1173     269    1146    4545

  Fragments arranged by size: 

                4545    3400    1173    1146     269      34
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2171    2312    2825    2872    3914    3983 
   Size:        197     926     228     141     513      47    1042      69

Cuts at:   3983    4559    4799    5582    8671    9572    9834     820 
   Size:        576     240     783    3089     901     262    1553

  Fragments arranged by size: 

                3089    1553    1042     926     901     783     576     513
                 262     240     228     197     141      69      47
 
SnaBI TAC'GTA

Cuts at:    493    8358     493 
   Size:       7865    2702
 
SpeI A'CTAG_T

Cuts at:    152    6529     152 
   Size:       6377    4190
 
SphI G_CATG'C

Cuts at:   1132    6328    7925    1132 
   Size:       5196    1597    3774

  Fragments arranged by size: 

                5196    3774    1597
 
SspI AAT'ATT

Cuts at:      5      52    6119    8491       5 
   Size:         47    6067    2372    2081

  Fragments arranged by size: 

                6067    2372    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2492    3923    5393    6296    1517 
   Size:        975    1431    1470     903    5788

  Fragments arranged by size: 

                5788    1470    1431     975     903
 
StyI C'CwwG_G

Cuts at:    513    1065    2736    4073    4740    4923    6761    6798    7014 
   Size:        552    1671    1337     667     183    1838      37     216

Cuts at:   7014    7470    7533    8006     513 
   Size:        456      63     473    3074

  Fragments arranged by size: 

                3074    1838    1671    1337     667     552     473     456
                 216     183      63      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2558    3568    4850    5525    5671    7874    8594 
   Size:       1255     978    1010    1282     675     146    2203     720

Cuts at:   8594    8611    8764    8949    9394     325 
   Size:         17     153     185     445    1498

  Fragments arranged by size: 

                2203    1498    1282    1255    1010     978     720     675
                 445     185     153     146      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3383    4878    6573 
   Size:        274      80      33      51    1443    1406    1495    1695

Cuts at:   6573    7770    8813   10525      96 
   Size:       1197    1043    1712     138

  Fragments arranged by size: 

                1712    1695    1495    1443    1406    1197    1043     274
                 138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2168    2200    2915    2947    5203    5235    6068    6100   10527 
   Size:         32     715      32    2256      32     833      32    4427

Cuts at:  10527   10559    2168 
   Size:         32    2176

  Fragments arranged by size: 

                4427    2256    2176     833     715      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    5345    1316 
   Size:       4029    6538
 
XbaI T'CTAG_A

Cuts at:   7903    7903 
   Size:      10567
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3363    5566    3363 
   Size:       2203    8364
 
XhoI C'TCGA_G

Cuts at:   1943    2872    1943 
   Size:        929    9638
 
XmaI C'CCGG_G

Cuts at:   1870    5270    5304    6477    6746    7892    1870 
   Size:       3400      34    1173     269    1146    4545

  Fragments arranged by size: 

                4545    3400    1173    1146     269      34
 
XmnI GAAnn'nnTTC

Cuts at:   4427    7364    8696    4427 
   Size:       2937    1332    6298

  Fragments arranged by size: 

                6298    2937    1332
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    8375     276 
   Size:         53      83     186     936    6841    2468

  Fragments arranged by size: 

                6841    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII     BaeI    BfrBI    BsiWI  BstZ17I     FseI    FspAI
     NruI     NsiI     PacI    RsrII     SfiI     SrfI     SwaI
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