Restriction map for
pFN21AE0032
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbrPI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
KpnI KspI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 476 GTCTAC GT^[AC][GT]AC
AccIII 290 TCCGGA T^CCGGA
AcyI 340 GGCGCC G[GA]^CG[CT]C
AhdI 541 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1481 GGGCCC GGGCC^C
AspEI 541 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1786 GAGCTC G[AT]GC[AT]^C
AspI 850 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1013 TGGCCA TGG^CCA
BbeI 340 GGCGCC GGCGC^C
BcgI 201 GCACAGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 340 GGCGCC G[GA]^CG[CT]C
BsaI 175 GAGACC GGTCTC(1/5)
BsaWI 290 TCCGGA [AT]^CCGG[AT]
BseAI 290 TCCGGA T^CCGGA
BsiHKAI 1786 GAGCTC G[AT]GC[AT]^C
BspEI 290 TCCGGA T^CCGGA
BspHI 1250 TCATGA T^CATGA
BsrBI 15 CCGCTC CCGCTC(-3/-3)
BsrDI 978 CATTGC GCAATG(2/0)
BssSI 885 CACGAG CACGAG(-5/-1)
BstAPI 1689 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1348 GGTGACC G^GT[ACGT]ACC
BstPI 1348 GGTGACC G^GT[ACGT]ACC
BstXI 1004 CCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 3 GACTGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 541 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1348 GGTGACC G^GT[ACGT]ACC
Hin1I 340 GGCGCC G[GA]^CG[CT]C
HpaI 1317 GTTAAC GTT^AAC
KasI 340 GGCGCC G^GCGCC
MaeII 698 ACGT A^CGT
MluNI 1013 TGGCCA TGG^CCA
MroI 290 TCCGGA T^CCGGA
MscI 1013 TGGCCA TGG^CCA
NarI 340 GGCGCC GG^CGCC
PaeR7I 1789 CTCGAG C^TCGAG
PflFI 850 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 482 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1250 TCATGA T^CATGA
SacI 1786 GAGCTC GAGCT^C
ScaI 1610 AGTACT AGT^ACT
SfoI 340 GGCGCC GGC^GCC
StuI 1289 AGGCCT AGG^CCT
Tth111I 850 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1789 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 215 CGCG CG^CG
225 CGCG
BamHI 626 GGATCC G^GATCC
1386 GGATCC
BanI 340 GGCGCC G^G[CT][GA]CC
1154 GGCACC
BbsI 894 GTCTTC GAAGAC(2/6)
1141 GTCTTC
BbvCI 567 CCTCAGC CCTCAGC(-5/-2)
1356 GCTGAGG
BfaI 394 CTAG C^TAG
1199 CTAG
BpmI 1747 CTCCAG CTGGAG(16/14)
752 CTGGAG
BpuAI 894 GTCTTC GAAGAC(2/6)
1141 GTCTTC
BstUI 215 CGCG CG^CG
225 CGCG
EcoRI 371 GAATTC G^AATTC
1225 GAATTC
HaeII 340 GGCGCC [GA]GCGC^[CT]
1764 GGCGCT
MaeI 394 CTAG C^TAG
1199 CTAG
MvnI 215 CGCG CG^CG
225 CGCG
NaeI 161 GCCGGC GCC^GGC
582 GCCGGC
NgoMIV 161 GCCGGC G^CCGGC
582 GCCGGC
PstI 1306 CTGCAG CTGCA^G
1696 CTGCAG
SfaNI 572 GCATC GCATC(5/9)
1509 GCATC
SmaI 126 CCCGGG CCC^GGG
1478 CCCGGG
SmlI 1525 CTCAAG C^T[CT][GA]AG
1789 CTCGAG
TaqI 1676 TCGA T^CGA
1790 TCGA
TfiI 430 GAATC G^A[AT]TC
1089 GATTC
TthHB8I 1676 TCGA T^CGA
1790 TCGA
XmaCI 126 CCCGGG C^CCGGG
1478 CCCGGG
XmaI 126 CCCGGG C^CCGGG
1478 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BanII
BbvI BcnI BglI BlpI BmyI
Bpu1102I BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII