(Circular) (Six-base) MAPSORT of: pFN21AE0032.seq Check: 2331 from: 1 to: 6526 pFN21AE0032 6526 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2305 2305 Size: 6526 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2482 3869 1937 Size: 545 1387 4594 Fragments arranged by size: 4594 1387 545 Acc65I G'GTAC_C Cuts at: 3847 3847 Size: 6526 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2734 3758 3825 4589 5973 6241 1632 Size: 1102 1024 67 764 1384 268 1917 Fragments arranged by size: 1917 1384 1102 1024 764 268 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 5425 6402 1203 Size: 4222 977 1327 Fragments arranged by size: 4222 1327 977 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 2551 5255 2551 Size: 2704 3822 AleI CACnn'nnGTG Cuts at: 1166 2498 2640 2894 3014 1166 Size: 1332 142 254 120 4678 Fragments arranged by size: 4678 1332 254 142 120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6526 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2452 2484 2452 Size: 32 6494 AlwNI CAG_nnn'CTG Cuts at: 2143 2320 3172 4269 5841 2143 Size: 177 852 1097 1572 2828 Fragments arranged by size: 2828 1572 1097 852 177 ApaI G_GGCC'C Cuts at: 3490 3490 Size: 6526 ApaLI G'TGCA_C Cuts at: 4583 5739 4583 Size: 1156 5370 ApoI r'AATT_y Cuts at: 1493 2376 3230 3572 3835 4061 4213 6322 1493 Size: 883 854 342 263 226 152 2109 1697 Fragments arranged by size: 2109 1697 883 854 342 263 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2131 3483 3794 3851 1870 Size: 73 188 1352 311 57 4545 Fragments arranged by size: 4545 1352 311 188 73 57 BamHI G'GATC_C Cuts at: 2631 3391 3856 4223 2631 Size: 760 465 367 4934 Fragments arranged by size: 4934 760 465 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 3159 3847 5302 618 Size: 325 217 627 558 814 688 1455 1842 Fragments arranged by size: 1842 1455 814 688 627 558 325 217 BanII G_rGCy'C Cuts at: 1391 2430 3490 3736 3795 3845 1391 Size: 1039 1060 246 59 50 4072 Fragments arranged by size: 4072 1060 1039 246 59 50 BbeI G_GCGC'C Cuts at: 2349 2349 Size: 6526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2892 3139 928 Size: 971 993 247 4315 Fragments arranged by size: 4315 993 971 247 BbvCI CC'TCA_GC Cuts at: 2573 3362 2573 Size: 789 5737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2195 2229 4717 4751 1903 Size: 34 258 34 2488 34 3678 Fragments arranged by size: 3678 2488 258 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5628 4418 Size: 1210 5316 BclI T'GATC_A Cuts at: 1548 1548 Size: 6526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2604 3075 3302 Size: 107 122 71 631 605 932 471 227 Cuts at: 3302 3526 3670 5137 136 Size: 224 144 1467 1525 Fragments arranged by size: 1525 1467 932 631 605 471 227 224 144 122 107 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 2225 2992 3596 3943 2225 Size: 767 604 347 4808 Fragments arranged by size: 4808 767 604 347 Bme1580I G_kGCm'C Cuts at: 1790 3490 4587 5743 1790 Size: 1700 1097 1156 2573 Fragments arranged by size: 2573 1700 1156 1097 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 2791 3628 Size: 416 53 83 399 24 658 716 837 Cuts at: 3628 4257 5215 442 Size: 629 958 1753 Fragments arranged by size: 1753 958 837 716 658 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2778 3737 5186 1239 Size: 1539 959 1449 2579 Fragments arranged by size: 2579 1539 1449 959 Bpu10I CC'TnA_GC Cuts at: 2424 2573 3362 2424 Size: 149 789 5588 Fragments arranged by size: 5588 789 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3515 4651 5516 5814 3515 Size: 1136 865 298 4227 Fragments arranged by size: 4227 1136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 5189 882 Size: 617 675 3015 2219 Fragments arranged by size: 3015 2219 675 617 BsaAI yAC'GTr Cuts at: 493 1204 4317 493 Size: 711 3113 2702 Fragments arranged by size: 3113 2702 711 BsaBI GATnn'nnATC Cuts at: 6520 6520 Size: 6526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2346 4333 4715 5350 Size: 53 83 186 936 813 1987 382 635 Cuts at: 5350 275 Size: 1451 Fragments arranged by size: 1987 1451 936 813 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2295 4959 5357 5631 5778 6189 2295 Size: 2664 398 274 147 411 2632 Fragments arranged by size: 2664 2632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2495 2525 1178 Size: 30 165 30 1092 30 5179 Fragments arranged by size: 5179 1092 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3104 3383 3602 3704 3812 1186 Size: 1918 279 219 102 108 3900 Fragments arranged by size: 3900 1918 279 219 108 102 BseYI C'CCAG_C Cuts at: 2098 2320 2814 3521 5729 6225 2098 Size: 222 494 707 2208 496 2399 Fragments arranged by size: 2399 2208 707 496 494 222 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 2790 3438 1163 Size: 573 453 601 648 4251 Fragments arranged by size: 4251 648 601 573 453 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3976 4737 5765 1540 Size: 275 186 1975 761 1028 2301 Fragments arranged by size: 2301 1975 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 4587 4672 5743 3795 Size: 792 85 1071 4578 Fragments arranged by size: 4578 1071 792 85 BsmI GAATG_Cn' Cuts at: 4037 4130 4037 Size: 93 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 3490 3736 3795 3845 4587 4672 Size: 399 640 1060 246 59 50 742 85 Cuts at: 4672 5743 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1060 742 640 399 246 85 59 50 BspEI T'CCGG_A Cuts at: 2295 6189 2295 Size: 3894 2632 BspHI T'CATG_A Cuts at: 3255 4415 6145 3255 Size: 1160 1730 3636 Fragments arranged by size: 3636 1730 1160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 2622 3366 3708 3881 844 Size: 1461 317 744 342 173 3489 Fragments arranged by size: 3489 1461 744 342 317 173 BsrBI CCG'CTC Cuts at: 1394 1823 2022 4413 1394 Size: 429 199 2391 3507 Fragments arranged by size: 3507 2391 429 199 BsrDI GCAATG_nn' Cuts at: 65 1248 2982 5021 5195 65 Size: 1183 1734 2039 174 1396 Fragments arranged by size: 2039 1734 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2166 2587 2617 2774 5170 5357 1700 Size: 110 356 421 30 157 2396 187 2869 Fragments arranged by size: 2869 2396 421 356 187 157 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 2890 4586 5598 2890 Size: 1696 1012 3818 Fragments arranged by size: 3818 1696 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3700 794 Size: 2906 3620 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 3353 1184 Size: 2169 4357 BstXI CCAn_nnnn'nTGG Cuts at: 3016 3016 Size: 6526 BstYI r'GATC_y Cuts at: 1972 2631 3261 3391 3783 3856 4223 4608 4625 Size: 659 630 130 392 73 367 385 17 Cuts at: 4625 6077 6163 6175 6521 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 659 630 392 385 367 346 130 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2635 2938 3009 513 Size: 552 1570 303 71 4030 Fragments arranged by size: 4030 1570 552 303 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5787 Fragments arranged by size: 5787 683 56 BtsI GCAGTG_nn' Cuts at: 2116 2505 4042 4836 4856 2116 Size: 389 1537 794 20 3786 Fragments arranged by size: 3786 1537 794 389 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6526 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6491 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 5533 809 Size: 507 698 3519 1802 Fragments arranged by size: 3519 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2472 3018 3671 Size: 54 1425 50 275 18 642 546 653 Cuts at: 3671 3973 4862 6491 8 Size: 302 889 1629 43 Fragments arranged by size: 1629 1425 889 653 642 546 302 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3973 1537 Size: 275 2161 4090 Fragments arranged by size: 4090 2161 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2939 3579 3801 4456 1353 Size: 1586 640 222 655 3423 Fragments arranged by size: 3423 1586 655 640 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2153 3446 5113 5487 5633 387 Size: 1064 138 564 1293 1667 374 146 1280 Fragments arranged by size: 1667 1293 1280 1064 564 374 146 138 EcoICRI GAG'CTC Cuts at: 3793 3793 Size: 6526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2734 2778 3737 3758 3825 4589 5186 Size: 393 1102 44 959 21 67 764 597 Cuts at: 5186 5973 6241 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1102 959 787 764 597 393 268 67 44 21 EcoO109I rG'GnC_Cy Cuts at: 2488 3055 3487 2488 Size: 567 432 5527 Fragments arranged by size: 5527 567 432 EcoRI G'AATT_C Cuts at: 2376 3230 3835 4213 2376 Size: 854 605 378 4689 Fragments arranged by size: 4689 854 605 378 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 2349 3773 5673 1678 Size: 671 1424 1900 2531 Fragments arranged by size: 2531 1900 1424 671 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 2990 3022 3325 Size: 32 135 32 965 32 421 32 303 Cuts at: 3325 3357 5167 5199 5241 5273 1373 Size: 32 1810 32 42 32 2626 Fragments arranged by size: 2626 1810 965 421 303 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3046 3220 3324 3870 4116 669 Size: 988 281 1108 174 104 546 246 3079 Fragments arranged by size: 3079 1108 988 546 281 246 174 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 3324 4116 3324 Size: 792 5734 KasI G'GCGC_C Cuts at: 2345 2345 Size: 6526 KpnI G_GTAC'C Cuts at: 3851 3851 Size: 6526 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2568 5640 5824 1305 Size: 1263 3072 184 2007 Fragments arranged by size: 3072 2007 1263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3020 10 Size: 54 1425 343 1188 3516 Fragments arranged by size: 3516 1425 1188 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 2598 2640 2894 3014 3443 4484 Size: 648 1332 100 42 254 120 429 1041 Cuts at: 4484 4843 5002 518 Size: 359 159 2042 Fragments arranged by size: 2042 1332 1041 648 429 359 254 159 120 100 42 MspA1I CmG'CkG Cuts at: 1606 2068 2407 2996 3525 3676 3706 3942 4619 Size: 462 339 589 529 151 30 236 677 Cuts at: 4619 5767 6012 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 529 462 339 245 236 151 30 NaeI GCC'GGC Cuts at: 2168 2589 2168 Size: 421 6105 NarI GG'CG_CC Cuts at: 2346 2346 Size: 6526 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5974 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NgoMIV G'CCGG_C Cuts at: 2166 2587 2166 Size: 421 6105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 3884 5429 1132 Size: 97 2655 1545 2229 Fragments arranged by size: 2655 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6526 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6526 PfoI T'CCnGG_A Cuts at: 3417 3786 3417 Size: 369 6157 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2488 Size: 6526 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PspOMI G'GGCC_C Cuts at: 3486 3486 Size: 6526 PspXI vC'TCGA_Gb Cuts at: 3794 3794 Size: 6526 PstI C_TGCA'G Cuts at: 830 3315 3705 3878 830 Size: 2485 390 173 3478 Fragments arranged by size: 3478 2485 390 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 PvuII CAG'CTG Cuts at: 2996 3525 3706 2996 Size: 529 181 5816 Fragments arranged by size: 5816 529 181 SacI G_AGCT'C Cuts at: 3795 3795 Size: 6526 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 3617 4774 6486 3617 Size: 1157 1712 3657 Fragments arranged by size: 3657 1712 1157 SfcI C'TryA_G Cuts at: 826 1045 2483 3311 3701 3713 3874 5009 5690 Size: 219 1438 828 390 12 161 1135 681 Cuts at: 5690 5881 826 Size: 191 1471 Fragments arranged by size: 1471 1438 1135 828 681 390 219 191 161 12 SfoI GGC'GCC Cuts at: 2347 2347 Size: 6526 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 2133 3485 3853 1872 Size: 261 1352 368 4545 Fragments arranged by size: 4545 1352 368 261 SmlI C'TyrA_G Cuts at: 820 1017 1943 3530 3794 4630 5531 5793 820 Size: 197 926 1587 264 836 901 262 1553 Fragments arranged by size: 1587 1553 926 901 836 264 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 3296 1517 Size: 1779 4747 StyI C'CwwG_G Cuts at: 513 1065 2093 2694 2926 3249 3965 513 Size: 552 1028 601 232 323 716 3074 Fragments arranged by size: 3074 1028 716 601 552 323 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2135 2480 2723 4553 4570 4723 Size: 1255 470 85 345 243 1830 17 153 Cuts at: 4723 4908 5353 325 Size: 185 445 1498 Fragments arranged by size: 1830 1498 1255 470 445 345 243 185 153 85 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3372 3495 3615 Size: 274 80 33 51 1443 1395 123 120 Cuts at: 3615 4772 6484 96 Size: 1157 1712 138 Fragments arranged by size: 1712 1443 1395 1157 274 138 123 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2623 2655 6486 6518 2623 Size: 32 3831 32 2631 Fragments arranged by size: 3831 2631 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2858 1316 Size: 1542 4984 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XhoI C'TCGA_G Cuts at: 1943 3794 1943 Size: 1851 4675 XmaI C'CCGG_G Cuts at: 1870 2131 3483 3851 1870 Size: 261 1352 368 4545 Fragments arranged by size: 4545 1352 368 261 XmnI GAAnn'nnTTC Cuts at: 4655 4655 Size: 6526 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI