Restriction map for
pFN21AE0032
(Linear) (Six-base) MAPSORT of: KIEE0032.seq Check: 2170 from: 1 to: 1821
KIEE0032.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 300 1821
Size: 300 1521
AccI GT'mk_AC
Cuts at: 0 477 1821
Size: 477 1344
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 1753 1820 1821
Size: 729 1024 67 1
Fragments arranged by size:
1024 729 67 1
AhdI GACnn_n'nnGTC
Cuts at: 0 546 1821
Size: 546 1275
AleI CACnn'nnGTG
Cuts at: 0 493 635 889 1009 1821
Size: 493 142 254 120 812
Fragments arranged by size:
812 493 254 142 120
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 1821
Size: 447 32 1342
Fragments arranged by size:
1342 447 32
AlwNI CAG_nnn'CTG
Cuts at: 0 138 315 1167 1821
Size: 138 177 852 654
Fragments arranged by size:
852 654 177 138
ApaI G_GGCC'C
Cuts at: 0 1485 1821
Size: 1485 336
ApoI r'AATT_y
Cuts at: 0 371 1225 1567 1821
Size: 371 854 342 254
Fragments arranged by size:
854 371 342 254
AvaI C'yCGr_G
Cuts at: 0 126 1478 1789 1821
Size: 126 1352 311 32
Fragments arranged by size:
1352 311 126 32
BamHI G'GATC_C
Cuts at: 0 626 1386 1821
Size: 626 760 435
Fragments arranged by size:
760 626 435
BanI G'GyrC_C
Cuts at: 0 340 1154 1821
Size: 340 814 667
Fragments arranged by size:
814 667 340
BanII G_rGCy'C
Cuts at: 0 425 1485 1731 1790 1821
Size: 425 1060 246 59 31
Fragments arranged by size:
1060 425 246 59 31
BbeI G_GCGC'C
Cuts at: 0 344 1821
Size: 344 1477
BbsI GAAGACnn'nnnn_
Cuts at: 0 887 1134 1821
Size: 887 247 687
Fragments arranged by size:
887 687 247
BbvCI CC'TCA_GC
Cuts at: 0 568 1357 1821
Size: 568 789 464
Fragments arranged by size:
789 568 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 224 1821
Size: 190 34 1597
Fragments arranged by size:
1597 190 34
BglI GCCn_nnn'nGGC
Cuts at: 0 599 1070 1297 1521 1665 1821
Size: 599 471 227 224 144 156
Fragments arranged by size:
599 471 227 224 156 144
BlpI GC'TnA_GC
Cuts at: 0 220 987 1591 1821
Size: 220 767 604 230
Fragments arranged by size:
767 604 230 220
Bme1580I G_kGCm'C
Cuts at: 0 1485 1821
Size: 1485 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 786 1623 1821
Size: 70 716 837 198
Fragments arranged by size:
837 716 198 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1732 1821
Size: 773 959 89
Fragments arranged by size:
959 773 89
Bpu10I CC'TnA_GC
Cuts at: 0 419 568 1357 1821
Size: 419 149 789 464
Fragments arranged by size:
789 464 419 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 1821
Size: 1510 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 1821
Size: 169 1652
BsaHI Gr'CG_yC
Cuts at: 0 341 1821
Size: 341 1480
BsaWI w'CCGG_w
Cuts at: 0 290 1821
Size: 290 1531
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 1821
Size: 490 30 1301
Fragments arranged by size:
1301 490 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1099 1378 1597 1699 1807 1821
Size: 1099 279 219 102 108 14
Fragments arranged by size:
1099 279 219 108 102 14
BseYI C'CCAG_C
Cuts at: 0 93 315 809 1516 1821
Size: 93 222 494 707 305
Fragments arranged by size:
707 494 305 222 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 785 1433 1821
Size: 184 601 648 388
Fragments arranged by size:
648 601 388 184
BsiHKAI G_wGCw'C
Cuts at: 0 1790 1821
Size: 1790 31
Bsp1286I G_dGCh'C
Cuts at: 0 425 1485 1731 1790 1821
Size: 425 1060 246 59 31
Fragments arranged by size:
1060 425 246 59 31
BspEI T'CCGG_A
Cuts at: 0 290 1821
Size: 290 1531
BspHI T'CATG_A
Cuts at: 0 1250 1821
Size: 1250 571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 617 1361 1703 1821
Size: 300 317 744 342 118
Fragments arranged by size:
744 342 317 300 118
BsrBI CCG'CTC
Cuts at: 0 17 1821
Size: 17 1804
BsrDI GCAATG_nn'
Cuts at: 0 977 1821
Size: 977 844
BsrFI r'CCGG_y
Cuts at: 0 161 582 612 769 1821
Size: 161 421 30 157 1052
Fragments arranged by size:
1052 421 161 157 30
BssSI C'ACGA_G
Cuts at: 0 885 1821
Size: 885 936
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1695 1821
Size: 1695 126
BstEII G'GTnAC_C
Cuts at: 0 1348 1821
Size: 1348 473
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1011 1821
Size: 1011 810
BstYI r'GATC_y
Cuts at: 0 626 1256 1386 1778 1821
Size: 626 630 130 392 43
Fragments arranged by size:
630 626 392 130 43
BtgI C'CryG_G
Cuts at: 0 630 933 1004 1821
Size: 630 303 71 817
Fragments arranged by size:
817 630 303 71
BtsI GCAGTG_nn'
Cuts at: 0 111 500 1821
Size: 111 389 1321
Fragments arranged by size:
1321 389 111
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1821
Size: 9 1812
EaeI y'GGCC_r
Cuts at: 0 467 1013 1666 1821
Size: 467 546 653 155
Fragments arranged by size:
653 546 467 155
EarI CTCTTCn'nnn_
Cuts at: 0 934 1574 1796 1821
Size: 934 640 222 25
Fragments arranged by size:
934 640 222 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 148 1441 1821
Size: 148 1293 380
Fragments arranged by size:
1293 380 148
EcoICRI GAG'CTC
Cuts at: 0 1788 1821
Size: 1788 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 773 1732 1753 1820 1821
Size: 729 44 959 21 67 1
Fragments arranged by size:
959 729 67 44 21 1
EcoO109I rG'GnC_Cy
Cuts at: 0 483 1050 1482 1821
Size: 483 567 432 339
Fragments arranged by size:
567 483 432 339
EcoRI G'AATT_C
Cuts at: 0 371 1225 1821
Size: 371 854 596
Fragments arranged by size:
854 596 371
HaeII r_GCGC'y
Cuts at: 0 344 1768 1821
Size: 344 1424 53
Fragments arranged by size:
1424 344 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 985 1017 1320 1352 1821
Size: 532 32 421 32 303 32 469
Fragments arranged by size:
532 469 421 303 32 32 32
HincII GTy'rAC
Cuts at: 0 1041 1215 1319 1821
Size: 1041 174 104 502
Fragments arranged by size:
1041 502 174 104
HpaI GTT'AAC
Cuts at: 0 1319 1821
Size: 1319 502
KasI G'GCGC_C
Cuts at: 0 340 1821
Size: 340 1481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1821
Size: 563 1258
MscI TGG'CCA
Cuts at: 0 1015 1821
Size: 1015 806
MslI CAynn'nnrTG
Cuts at: 0 493 593 635 889 1009 1438 1821
Size: 493 100 42 254 120 429 383
Fragments arranged by size:
493 429 383 254 120 100 42
MspA1I CmG'CkG
Cuts at: 0 63 402 991 1520 1671 1701 1821
Size: 63 339 589 529 151 30 120
Fragments arranged by size:
589 529 339 151 120 63 30
NaeI GCC'GGC
Cuts at: 0 163 584 1821
Size: 163 421 1237
Fragments arranged by size:
1237 421 163
NarI GG'CG_CC
Cuts at: 0 341 1821
Size: 341 1480
NgoMIV G'CCGG_C
Cuts at: 0 161 582 1821
Size: 161 421 1239
Fragments arranged by size:
1239 421 161
PfoI T'CCnGG_A
Cuts at: 0 1412 1781 1821
Size: 1412 369 40
Fragments arranged by size:
1412 369 40
PpuMI rG'GwC_Cy
Cuts at: 0 483 1821
Size: 483 1338
PspOMI G'GGCC_C
Cuts at: 0 1481 1821
Size: 1481 340
PspXI vC'TCGA_Gb
Cuts at: 0 1789 1821
Size: 1789 32
PstI C_TGCA'G
Cuts at: 0 1310 1700 1821
Size: 1310 390 121
Fragments arranged by size:
1310 390 121
PvuII CAG'CTG
Cuts at: 0 991 1520 1701 1821
Size: 991 529 181 120
Fragments arranged by size:
991 529 181 120
SacI G_AGCT'C
Cuts at: 0 1790 1821
Size: 1790 31
ScaI AGT'ACT
Cuts at: 0 1612 1821
Size: 1612 209
SfcI C'TryA_G
Cuts at: 0 478 1306 1696 1708 1821
Size: 478 828 390 12 113
Fragments arranged by size:
828 478 390 113 12
SfoI GGC'GCC
Cuts at: 0 342 1821
Size: 342 1479
SmaI CCC'GGG
Cuts at: 0 128 1480 1821
Size: 128 1352 341
Fragments arranged by size:
1352 341 128
SmlI C'TyrA_G
Cuts at: 0 1525 1789 1821
Size: 1525 264 32
Fragments arranged by size:
1525 264 32
StuI AGG'CCT
Cuts at: 0 1291 1821
Size: 1291 530
StyI C'CwwG_G
Cuts at: 0 88 689 921 1244 1821
Size: 88 601 232 323 577
Fragments arranged by size:
601 577 323 232 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 130 475 718 1821
Size: 45 85 345 243 1103
Fragments arranged by size:
1103 345 243 85 45
TatI w'GTAC_w
Cuts at: 0 1367 1490 1610 1821
Size: 1367 123 120 211
Fragments arranged by size:
1367 211 123 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 618 650 1821
Size: 618 32 1171
Fragments arranged by size:
1171 618 32
Tth111I GACn'n_nGTC
Cuts at: 0 853 1821
Size: 853 968
XhoI C'TCGA_G
Cuts at: 0 1789 1821
Size: 1789 32
XmaI C'CCGG_G
Cuts at: 0 126 1478 1821
Size: 126 1352 343
Fragments arranged by size:
1352 343 126
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII HpaI KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PfoI PpuMI PspOMI PspXI PstI PvuII
SacI ScaI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI
BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BsiEI
BsiWI BsmI BsmBI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EcoNI EcoRV
FalI FseI FspI FspAI HindIII KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XcmI XmnI ZraI