Restriction map for
pFN21AB8875
(Circular) (Six-base) MAPSORT of: pFN21AB8875.seq Check: 4345 from: 1 to: 5203
pFN21AB8875 5203 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3013 278
Size: 53 83 186 936 1477 2468
Fragments arranged by size:
2468 1477 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2546 1937
Size: 609 4594
Acc65I G'GTAC_C
Cuts at: 2225 2524 2225
Size: 299 4904
AclI AA'CG_TT
Cuts at: 1563 3330 3703 1563
Size: 1767 373 3063
Fragments arranged by size:
3063 1767 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2294 3266 4650 4918 1632
Size: 575 87 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 575 268 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5006
AflIII A'CryG_T
Cuts at: 1203 4102 5079 1203
Size: 2899 977 1327
Fragments arranged by size:
2899 1327 977
AgeI A'CCGG_T
Cuts at: 4034 4034
Size: 5203
AhdI GACnn_n'nnGTC
Cuts at: 3932 3932
Size: 5203
AleI CACnn'nnGTG
Cuts at: 1166 2366 1166
Size: 1200 4003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5203
AlwNI CAG_nnn'CTG
Cuts at: 2946 4518 2946
Size: 1572 3631
ApaLI G'TGCA_C
Cuts at: 3260 4416 3260
Size: 1156 4047
ApoI r'AATT_y
Cuts at: 1493 2512 2738 2890 4999 1493
Size: 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3757 160
Size: 872 2725 1606
Fragments arranged by size:
2725 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5203
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2528 1870
Size: 73 112 473 4545
Fragments arranged by size:
4545 473 112 73
BamHI G'GATC_C
Cuts at: 2533 2900 2533
Size: 367 4836
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 2225 2318 2524 3979
Size: 325 217 627 408 30 93 206 1455
Cuts at: 3979 618
Size: 1842
Fragments arranged by size:
1842 1455 627 408 325 217 206 93
30
BanII G_rGCy'C
Cuts at: 1391 2458 2522 1391
Size: 1067 64 4072
Fragments arranged by size:
4072 1067 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2152 2186 3394 3428 1903
Size: 34 50 34 131 34 1208 34 3678
Fragments arranged by size:
3678 1208 131 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3095 4305 3095
Size: 1210 3993
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5203
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3814 136
Size: 107 122 71 631 605 2142 1525
Fragments arranged by size:
2142 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5198 5198
Size: 5203
BlpI GC'TnA_GC
Cuts at: 2620 2620
Size: 5203
Bme1580I G_kGCm'C
Cuts at: 1790 2329 3264 4420 1790
Size: 539 935 1156 2573
Fragments arranged by size:
2573 1156 935 539
BmgBI CAC'GTC
Cuts at: 1318 2065 1318
Size: 747 4456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2934 3892 442
Size: 416 53 83 399 24 1517 958 1753
Fragments arranged by size:
1753 1517 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2472
Size: 5203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2198 3863 1239
Size: 794 165 1665 2579
Fragments arranged by size:
2579 1665 794 165
Bpu10I CC'TnA_GC
Cuts at: 2457 2457
Size: 5203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3328 4193 4491 3328
Size: 865 298 4040
Fragments arranged by size:
4040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2426 3866 882
Size: 617 927 1440 2219
Fragments arranged by size:
2219 1440 927 617
BsaAI yAC'GTr
Cuts at: 493 1204 2994 493
Size: 711 1790 2702
Fragments arranged by size:
2702 1790 711
BsaBI GATnn'nnATC
Cuts at: 2400 5197 2400
Size: 2797 2406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2412 3010 3392
Size: 53 83 186 936 579 300 598 382
Cuts at: 3392 4027 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 598 579 382 300 186
83 53
BsaWI w'CCGG_w
Cuts at: 2222 3636 4034 4308 4455 4866 2222
Size: 1414 398 274 147 411 2559
Fragments arranged by size:
2559 1414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4978
Fragments arranged by size:
4978 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 4359
BseYI C'CCAG_C
Cuts at: 4406 4902 4406
Size: 496 4707
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 2087 1163
Size: 573 336 15 4279
Fragments arranged by size:
4279 573 336 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 2653 3414 4442 1540
Size: 275 186 34 618 761 1028 2301
Fragments arranged by size:
2301 1028 761 618 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 2292 3264 3349 4420 2292
Size: 972 85 1071 3075
Fragments arranged by size:
3075 1071 972 85
BsmI GAATG_Cn'
Cuts at: 2714 2807 2714
Size: 93 5110
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2329 2458 2522 3264 3349 4420
Size: 399 502 37 129 64 742 85 1071
Cuts at: 4420 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 502 399 129 85 64
37
BspEI T'CCGG_A
Cuts at: 4866 4866
Size: 5203
BspHI T'CATG_A
Cuts at: 2148 3092 4822 2148
Size: 944 1730 2529
Fragments arranged by size:
2529 1730 944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2558 844
Size: 1290 424 3489
Fragments arranged by size:
3489 1290 424
BsrBI CCG'CTC
Cuts at: 1394 1823 3090 1394
Size: 429 1267 3507
Fragments arranged by size:
3507 1267 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3698 3872 65
Size: 1183 2450 174 1396
Fragments arranged by size:
2450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2034 3847 4034 1700
Size: 110 217 7 1813 187 2869
Fragments arranged by size:
2869 1813 217 187 110 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5203
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5203
BssSI C'ACGA_G
Cuts at: 2465 3263 4275 2465
Size: 798 1012 3393
Fragments arranged by size:
3393 1012 798
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 794
Size: 1280 3923
BstBI TT'CG_AA
Cuts at: 4007 4007
Size: 5203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5203
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2176 2011
Size: 165 5038
BstYI r'GATC_y
Cuts at: 1972 2533 2900 3285 3302 4754 4840 4852 5198
Size: 561 367 385 17 1452 86 12 346
Cuts at: 5198 1972
Size: 1977
Fragments arranged by size:
1977 1452 561 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4651
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2155 2387 487
Size: 683 56 929 232 3303
Fragments arranged by size:
3303 929 683 232 56
BtsI GCAGTG_nn'
Cuts at: 2361 2441 2719 3513 3533 2361
Size: 80 278 794 20 4031
Fragments arranged by size:
4031 794 278 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5168
DraI TTT'AAA
Cuts at: 2507 2507
Size: 5203
DraIII CAC_nnn'GTG
Cuts at: 2491 2491
Size: 5203
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4210 809
Size: 507 2894 1802
Fragments arranged by size:
2894 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2650 3539 5168
Size: 54 1425 50 275 18 820 889 1629
Cuts at: 5168 8
Size: 43
Fragments arranged by size:
1629 1425 889 820 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2650 1537
Size: 275 838 4090
Fragments arranged by size:
4090 838 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3133 1353
Size: 1780 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3790 4164 4310 387
Size: 1064 138 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2198 2207 2294 3266 3863 4650
Size: 393 401 165 9 87 972 597 787
Cuts at: 4650 4918 1239
Size: 268 1524
Fragments arranged by size:
1524 972 787 597 401 393 268 165
87 9
EcoO109I rG'GnC_Cy
Cuts at: 2278 2461 2278
Size: 183 5020
EcoRI G'AATT_C
Cuts at: 2512 2890 2512
Size: 378 4825
FspI TGC'GCA
Cuts at: 3709 3709
Size: 5203
HaeII r_GCGC'y
Cuts at: 1678 2141 4350 1678
Size: 463 2209 2531
Fragments arranged by size:
2531 2209 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3844 3876 3918 3950 1373
Size: 32 135 32 2272 32 42 32 2626
Fragments arranged by size:
2626 2272 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 2547 2793 669
Size: 988 281 367 242 246 3079
Fragments arranged by size:
3079 988 367 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5203
HpaI GTT'AAC
Cuts at: 2793 2793
Size: 5203
KpnI G_GTAC'C
Cuts at: 2229 2528 2229
Size: 299 4904
MfeI C'AATT_G
Cuts at: 2802 2802
Size: 5203
MluI A'CGCG_T
Cuts at: 5079 5079
Size: 5203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4317 4501 1305
Size: 3012 184 2007
Fragments arranged by size:
3012 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3381
Fragments arranged by size:
3381 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2366 3161 3520 3679 518
Size: 648 1200 795 359 159 2042
Fragments arranged by size:
2042 1200 795 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2387 2500 2619 3296 4444 4689 1606
Size: 781 113 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 781 677 245 119 113
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 5203
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4651
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5203
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 5203
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5203
NotI GC'GGCC_GC
Cuts at: 2650 2650
Size: 5203
NspI r_CATG'y
Cuts at: 1132 1229 2561 4106 1132
Size: 97 1332 1545 2229
Fragments arranged by size:
2229 1545 1332 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5203
PciI A'CATG_T
Cuts at: 4102 4102
Size: 5203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2491 1108
Size: 1383 3820
PfoI T'CCnGG_A
Cuts at: 2211 2262 2211
Size: 51 5152
PmeI GTTT'AAAC
Cuts at: 2507 2507
Size: 5203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 4785 4817 3272
Size: 32 1481 32 3658
Fragments arranged by size:
3658 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2461 2278
Size: 183 5020
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5203
PsiI TTA'TAA
Cuts at: 2773 2773
Size: 5203
PstI C_TGCA'G
Cuts at: 830 2145 2448 2481 2555 830
Size: 1315 303 33 74 3478
Fragments arranged by size:
3478 1315 303 74 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5203
PvuII CAG'CTG
Cuts at: 2387 2387
Size: 5203
SalI G'TCGA_C
Cuts at: 1936 2545 1936
Size: 609 4594
SbfI CC_TGCA'GG
Cuts at: 2481 2555 2481
Size: 74 5129
ScaI AGT'ACT
Cuts at: 3451 5163 3451
Size: 1712 3491
SfcI C'TryA_G
Cuts at: 826 1045 2141 2444 2477 2551 3686 4367 4558
Size: 219 1096 303 33 74 1135 681 191
Cuts at: 4558 826
Size: 1471
Fragments arranged by size:
1471 1135 1096 681 303 219 191 74
33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5203
SgrAI Cr'CCGG_yG
Cuts at: 1700 2027 1700
Size: 327 4876
SmaI CCC'GGG
Cuts at: 1872 2057 2530 1872
Size: 185 473 4545
Fragments arranged by size:
4545 473 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3307 4208 4470 820
Size: 197 926 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2994 493
Size: 2501 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5203
SphI G_CATG'C
Cuts at: 1132 2561 1132
Size: 1429 3774
SspI AAT'ATT
Cuts at: 5 52 3127 5
Size: 47 3075 2081
Fragments arranged by size:
3075 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5203
StyI C'CwwG_G
Cuts at: 513 1065 2430 2642 513
Size: 552 1365 212 3074
Fragments arranged by size:
3074 1365 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3230 3247 3400 3585 4030 325
Size: 1255 1650 17 153 185 445 1498
Fragments arranged by size:
1650 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3449 5161 96
Size: 274 80 33 51 1443 1472 1712 138
Fragments arranged by size:
1712 1472 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 5163 5195 2319
Size: 32 2812 32 2327
Fragments arranged by size:
2812 2327 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5203
XbaI T'CTAG_A
Cuts at: 2539 2539
Size: 5203
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5203
XmaI C'CCGG_G
Cuts at: 1870 2055 2528 1870
Size: 185 473 4545
Fragments arranged by size:
4545 473 185
XmnI GAAnn'nnTTC
Cuts at: 3332 3332
Size: 5203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3011 276
Size: 53 83 186 936 1477 2468
Fragments arranged by size:
2468 1477 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI