################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV FseI FspI HaeII HincII HindII HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 539 GACGTC GACGT^C Acc65I 1816 GGTACC G^GTACC AccII 410 CGCG CG^CG AcyI 539 GACGTC G[GA]^CG[CT]C AseI 1348 ATTAAT AT^TAAT AsnI 1348 ATTAAT AT^TAAT Asp718I 1816 GGTACC G^GTACC BalI 221 TGGCCA TGG^CCA BamHI 162 GGATCC G^GATCC BanI 1816 GGTACC G^G[CT][GA]CC BsaAI 1390 TACGTG [CT]AC^GT[GA] BsaBI 385 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 539 GACGTC G[GA]^CG[CT]C BsgI 1909 GTGCAG GTGCAG(16/14) BsmFI 2216 GGGAC GGGAC(10/14) Bsp1407I 1203 TGTACA T^GTACA BspHI 2192 TCATGA T^CATGA BsrGI 1203 TGTACA T^GTACA BssSI 2177 CTCGTG CACGAG(-5/-1) BstEII 2167 GGTTACC G^GT[ACGT]ACC BstPI 2167 GGTTACC G^GT[ACGT]ACC BstUI 410 CGCG CG^CG BstXI 235 CCAACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 409 GCGC GCG^C DraI 2391 TTTAAA TTT^AAA DsaI 1944 CCATGG C^C[GA][CT]GG EaeI 221 TGGCCA [CT]^GGCC[GA] EcoO65I 2167 GGTTACC G^GT[ACGT]ACC EcoRI 1082 GAATTC G^AATTC EcoT22I 1609 ATGCAT ATGCA^T FauI 470 CCCGC CCCGC(4/6) HgaI 706 GACGC GACGC(5/10) HhaI 409 GCGC GCG^C Hin1I 539 GACGTC G[GA]^CG[CT]C HinP1I 409 GCGC G^CGC HindIII 1964 AAGCTT A^AGCTT KpnI 1816 GGTACC GGTAC^C MamI 385 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 221 TGGCCA TGG^CCA MscI 221 TGGCCA TGG^CCA MvnI 410 CGCG CG^CG NcoI 1944 CCATGG C^CATGG NsiI 1609 ATGCAT ATGCA^T PaeR7I 2086 CTCGAG C^TCGAG PflMI 259 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1348 ATTAAT AT^TAAT PstI 1087 CTGCAG CTGCA^G PvuII 578 CAGCTG CAG^CTG RcaI 2192 TCATGA T^CATGA SacI 1951 GAGCTC GAGCT^C SapI 1879 GAAGAGC GCTCTTC(1/4) ScaI 885 AGTACT AGT^ACT SexAI 1973 ACCAGGT A^CC[AT]GGT SspBI 1203 TGTACA T^GTACA Van91I 259 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 464 CCAGAGCCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2086 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 1831 GAGCAC G[AT]GC[AT]^C 1951 GAGCTC BbsI 177 GAAGAC GAAGAC(2/6) 2314 GAAGAC BclI 14 TGATCA T^GATCA 1736 TGATCA BglII 754 AGATCT A^GATCT 1629 AGATCT BpuAI 177 GAAGAC GAAGAC(2/6) 2314 GAAGAC BseRI 1325 CTCCTC GAGGAG(10/8) 617 GAGGAG BsiHKAI 1831 GAGCAC G[AT]GC[AT]^C 1951 GAGCTC BsmI 1607 GAATGC GAATGC(1/-1) 1987 GAATGC DrdI 180 GACAGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2388 GACTTTAAAGTC EcoT14I 663 CCTTGG C^C[AT][AT]GG 1944 CCATGG FbaI 14 TGATCA T^GATCA 1736 TGATCA MfeI 422 CAATTG C^AATTG 523 CAATTG MspA1I 62 CAGCGG C[AC]G^C[GT]G 578 CAGCTG MunI 422 CAATTG C^AATTG 523 CAATTG SpeI 844 ACTAGT A^CTAGT 882 ACTAGT StyI 663 CCTTGG C^C[AT][AT]GG 1944 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI