(Circular) (Six-base) MAPSORT of: pFN21AB8655.seq Check: 4097 from: 1 to: 6403 pFN21AB8655 6403 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2965 3429 2965 Size: 464 5939 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4213 278 Size: 53 83 186 936 2677 2468 Fragments arranged by size: 2677 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2247 2331 3746 1937 Size: 310 84 1415 4594 Fragments arranged by size: 4594 1415 310 84 Acc65I G'GTAC_C Cuts at: 3724 3724 Size: 6403 AclI AA'CG_TT Cuts at: 1563 4530 4903 1563 Size: 2967 373 3063 Fragments arranged by size: 3063 2967 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2389 2990 4466 5850 6118 1632 Size: 464 293 601 1476 1384 268 1917 Fragments arranged by size: 1917 1476 1384 601 464 293 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6206 AflIII A'CryG_T Cuts at: 1203 5302 6279 1203 Size: 4099 977 1327 Fragments arranged by size: 4099 1327 977 AgeI A'CCGG_T Cuts at: 5234 5234 Size: 6403 AhdI GACnn_n'nnGTC Cuts at: 5132 5132 Size: 6403 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3309 3437 3485 1184 Size: 2125 128 48 4102 Fragments arranged by size: 4102 2125 128 48 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2777 2809 2777 Size: 32 6371 AlwNI CAG_nnn'CTG Cuts at: 2162 3205 4146 5718 2162 Size: 1043 941 1572 2847 Fragments arranged by size: 2847 1572 1043 941 ApaLI G'TGCA_C Cuts at: 4460 5616 4460 Size: 1156 5247 ApoI r'AATT_y Cuts at: 1493 3019 3053 3712 3938 4090 6199 1493 Size: 1526 34 659 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 659 226 152 34 AseI AT'TA_AT Cuts at: 160 1032 4957 160 Size: 872 3925 1606 Fragments arranged by size: 3925 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 AvaI C'yCGr_G Cuts at: 1870 1943 3164 3728 1870 Size: 73 1221 564 4545 Fragments arranged by size: 4545 1221 564 73 BamHI G'GATC_C Cuts at: 3733 4100 3733 Size: 367 6036 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3724 5179 618 Size: 325 217 627 1937 1455 1842 Fragments arranged by size: 1937 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3164 3722 1391 Size: 1773 558 4072 Fragments arranged by size: 4072 1773 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2283 2403 2954 928 Size: 971 384 120 551 4377 Fragments arranged by size: 4377 971 551 384 120 BbvCI CC'TCA_GC Cuts at: 3657 3657 Size: 6403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3298 3332 4594 4628 1903 Size: 34 1361 34 1262 34 3678 Fragments arranged by size: 3678 1361 1262 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4295 5505 4295 Size: 1210 5193 BclI T'GATC_A Cuts at: 1548 1548 Size: 6403 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5014 136 Size: 107 122 71 631 605 3342 1525 Fragments arranged by size: 3342 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6398 6398 Size: 6403 BlpI GC'TnA_GC Cuts at: 3410 3820 3410 Size: 410 5993 Bme1580I G_kGCm'C Cuts at: 1790 3365 4464 5620 1790 Size: 1575 1099 1156 2573 Fragments arranged by size: 2573 1575 1156 1099 BmgBI CAC'GTC Cuts at: 1318 2083 3130 1318 Size: 765 1047 4591 Fragments arranged by size: 4591 1047 765 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4134 5092 442 Size: 416 53 83 399 24 2717 958 1753 Fragments arranged by size: 2717 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6403 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2573 2573 Size: 6403 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2921 3202 3351 5063 1239 Size: 1682 281 149 1712 2579 Fragments arranged by size: 2579 1712 1682 281 149 Bpu10I CC'TnA_GC Cuts at: 3178 3401 3494 3657 3178 Size: 223 93 163 5924 Fragments arranged by size: 5924 223 163 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 3410 4528 5393 5691 2213 Size: 1197 1118 865 298 2925 Fragments arranged by size: 2925 1197 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3533 5066 882 Size: 617 2034 1533 2219 Fragments arranged by size: 2219 2034 1533 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 2719 4194 493 Size: 711 933 582 1475 2702 Fragments arranged by size: 2702 1475 933 711 582 BsaBI GATnn'nnATC Cuts at: 6397 6397 Size: 6403 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4210 4592 5227 275 Size: 53 83 186 936 2677 382 635 1451 Fragments arranged by size: 2677 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 4836 5234 5508 5655 6066 2766 Size: 2070 398 274 147 411 3103 Fragments arranged by size: 3103 2070 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2229 2259 2777 2807 3200 Size: 30 165 30 826 30 518 30 393 Cuts at: 3200 3230 1178 Size: 30 4351 Fragments arranged by size: 4351 826 518 393 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 2504 2530 2795 1186 Size: 1246 72 26 265 4794 Fragments arranged by size: 4794 1246 265 72 26 BseYI C'CCAG_C Cuts at: 3282 5606 6102 3282 Size: 2324 496 3583 Fragments arranged by size: 3583 2324 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2788 3098 3602 1163 Size: 573 1052 310 504 3964 Fragments arranged by size: 3964 1052 573 504 310 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3853 4614 5642 1540 Size: 275 186 1852 761 1028 2301 Fragments arranged by size: 2301 1852 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2844 4464 4549 5620 2844 Size: 1620 85 1071 3627 Fragments arranged by size: 3627 1620 1071 85 BsmI GAATG_Cn' Cuts at: 2431 3090 3914 4007 2431 Size: 659 824 93 4827 Fragments arranged by size: 4827 824 659 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2844 3164 3365 3722 4464 4549 5620 Size: 399 1054 320 201 357 742 85 1071 Cuts at: 5620 1391 Size: 2174 Fragments arranged by size: 2174 1071 1054 742 399 357 320 201 85 BspEI T'CCGG_A Cuts at: 6066 6066 Size: 6403 BspHI T'CATG_A Cuts at: 4292 6022 4292 Size: 1730 4673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2965 3429 3758 844 Size: 2121 464 329 3489 Fragments arranged by size: 3489 2121 464 329 BsrBI CCG'CTC Cuts at: 1394 1823 4290 1394 Size: 429 2467 3507 Fragments arranged by size: 3507 2467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3291 4898 5072 65 Size: 1183 2043 1607 174 1396 Fragments arranged by size: 2043 1607 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3391 3453 5047 5234 1700 Size: 110 1581 62 1594 187 2869 Fragments arranged by size: 2869 1594 1581 187 110 62 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6403 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6403 BssSI C'ACGA_G Cuts at: 4463 5475 4463 Size: 1012 5391 BstAPI GCAn_nnn'nTGC Cuts at: 794 2509 794 Size: 1715 4688 BstBI TT'CG_AA Cuts at: 5207 5207 Size: 6403 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6403 BstXI CCAn_nnnn'nTGG Cuts at: 3130 3130 Size: 6403 BstYI r'GATC_y Cuts at: 1972 2106 2811 3733 4100 4485 4502 5954 6040 Size: 134 705 922 367 385 17 1452 86 Cuts at: 6040 6052 6398 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 922 705 385 367 346 134 86 17 12 BstZ17I GTA'TAC Cuts at: 2332 2332 Size: 6403 BtgI C'CryG_G Cuts at: 513 1065 2601 2796 2997 3244 3519 513 Size: 552 1536 195 201 247 275 3397 Fragments arranged by size: 3397 1536 552 275 247 201 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5664 Fragments arranged by size: 5664 683 56 BtsI GCAGTG_nn' Cuts at: 3919 4713 4733 3919 Size: 794 20 5589 Fragments arranged by size: 5589 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6403 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6368 DraI TTT'AAA Cuts at: 3707 3707 Size: 6403 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5410 809 Size: 507 4094 1802 Fragments arranged by size: 4094 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3120 3850 4739 Size: 54 1425 50 275 18 1290 730 889 Cuts at: 4739 6368 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1290 889 730 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3850 1537 Size: 275 2038 4090 Fragments arranged by size: 4090 2038 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2409 2421 2481 2542 3033 4333 1353 Size: 719 337 12 60 61 491 1300 3423 Fragments arranged by size: 3423 1300 719 491 337 61 60 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2822 4990 5364 5510 387 Size: 1064 138 1233 2168 374 146 1280 Fragments arranged by size: 2168 1280 1233 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2389 2921 2990 3202 3351 4466 Size: 393 464 293 532 69 212 149 1115 Cuts at: 4466 5063 5850 6118 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1115 787 597 532 464 393 293 268 212 149 69 EcoNI CCTnn'n_nnAGG Cuts at: 2972 2972 Size: 6403 EcoO109I rG'GnC_Cy Cuts at: 2059 2638 3332 2059 Size: 579 694 5130 Fragments arranged by size: 5130 694 579 EcoRI G'AATT_C Cuts at: 3712 4090 3712 Size: 378 6025 FspI TGC'GCA Cuts at: 4909 4909 Size: 6403 HaeII r_GCGC'y Cuts at: 1678 2746 3460 5550 1678 Size: 1068 714 2090 2531 Fragments arranged by size: 2531 2090 1068 714 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3200 3232 5044 5076 5118 Size: 32 135 32 1628 32 1812 32 42 Cuts at: 5118 5150 1373 Size: 32 2626 Fragments arranged by size: 2626 1812 1628 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3747 3993 669 Size: 988 281 1809 246 3079 Fragments arranged by size: 3079 1809 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6403 HpaI GTT'AAC Cuts at: 3993 3993 Size: 6403 KpnI G_GTAC'C Cuts at: 3728 3728 Size: 6403 MfeI C'AATT_G Cuts at: 4002 4002 Size: 6403 MluI A'CGCG_T Cuts at: 6279 6279 Size: 6403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2650 5517 5701 1305 Size: 1345 2867 184 2007 Fragments arranged by size: 2867 2007 1345 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3122 10 Size: 54 1425 343 1290 3291 Fragments arranged by size: 3291 1425 1290 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2606 2708 3074 4361 4720 4879 Size: 648 843 597 102 366 1287 359 159 Cuts at: 4879 518 Size: 2042 Fragments arranged by size: 2042 1287 843 648 597 366 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2524 3271 3300 3593 3819 4496 5644 5889 Size: 918 747 29 293 226 677 1148 245 Cuts at: 5889 1606 Size: 2120 Fragments arranged by size: 2120 1148 918 747 677 293 245 226 29 NaeI GCC'GGC Cuts at: 3393 3393 Size: 6403 NcoI C'CATG_G Cuts at: 513 1065 2601 2997 3244 513 Size: 552 1536 396 247 3672 Fragments arranged by size: 3672 1536 552 396 247 NdeI CA'TA_TG Cuts at: 387 387 Size: 6403 NgoMIV G'CCGG_C Cuts at: 3391 3391 Size: 6403 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6403 NotI GC'GGCC_GC Cuts at: 3850 3850 Size: 6403 NspI r_CATG'y Cuts at: 1132 1229 2653 3073 3761 5306 1132 Size: 97 1424 420 688 1545 2229 Fragments arranged by size: 2229 1545 1424 688 420 97 PasI CC'CwG_GG Cuts at: 1348 3328 1348 Size: 1980 4423 PciI A'CATG_T Cuts at: 5302 5302 Size: 6403 PflMI CCAn_nnn'nTGG Cuts at: 1108 2162 2998 3205 3250 1108 Size: 1054 836 207 45 4261 Fragments arranged by size: 4261 1054 836 207 45 PfoI T'CCnGG_A Cuts at: 2937 3218 3608 2937 Size: 281 390 5732 Fragments arranged by size: 5732 390 281 PmeI GTTT'AAAC Cuts at: 3707 3707 Size: 6403 PmlI CAC'GTG Cuts at: 2137 2137 Size: 6403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2777 2809 4472 4504 5985 6017 2777 Size: 32 1663 32 1481 32 3163 Fragments arranged by size: 3163 1663 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2638 2059 Size: 579 5824 PshAI GACnn'nnGTC Cuts at: 1613 2958 1613 Size: 1345 5058 PsiI TTA'TAA Cuts at: 3973 3973 Size: 6403 PstI C_TGCA'G Cuts at: 830 2742 3755 830 Size: 1912 1013 3478 Fragments arranged by size: 3478 1912 1013 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6403 PvuII CAG'CTG Cuts at: 3271 3300 3271 Size: 29 6374 SalI G'TCGA_C Cuts at: 1936 3745 1936 Size: 1809 4594 SapI GCTCTTCn'nnn_ Cuts at: 2421 2421 Size: 6403 SbfI CC_TGCA'GG Cuts at: 3755 3755 Size: 6403 ScaI AGT'ACT Cuts at: 4651 6363 4651 Size: 1712 4691 SfcI C'TryA_G Cuts at: 826 1045 2738 3751 4886 5567 5758 826 Size: 219 1693 1013 1135 681 191 1471 Fragments arranged by size: 1693 1471 1135 1013 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6403 SmaI CCC'GGG Cuts at: 1872 3730 1872 Size: 1858 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 3425 4507 5408 5670 820 Size: 197 926 249 1233 1082 901 262 1553 Fragments arranged by size: 1553 1233 1082 926 901 262 249 197 SnaBI TAC'GTA Cuts at: 493 4194 493 Size: 3701 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6403 SphI G_CATG'C Cuts at: 1132 3761 1132 Size: 2629 3774 SspI AAT'ATT Cuts at: 5 52 4327 5 Size: 47 4275 2081 Fragments arranged by size: 4275 2081 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 5803 StyI C'CwwG_G Cuts at: 513 1065 2601 2634 2997 3244 3842 513 Size: 552 1536 33 363 247 598 3074 Fragments arranged by size: 3074 1536 598 552 363 247 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2154 2318 2677 3296 3557 4430 4447 Size: 1255 574 164 359 619 261 873 17 Cuts at: 4447 4600 4785 5230 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 873 619 574 445 359 261 185 164 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3342 4649 6361 Size: 274 80 33 51 1443 1365 1307 1712 Cuts at: 6361 96 Size: 138 Fragments arranged by size: 1712 1443 1365 1307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 6363 6395 2078 Size: 32 4253 32 2086 Fragments arranged by size: 4253 2086 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2593 3131 1316 Size: 1277 538 4588 Fragments arranged by size: 4588 1277 538 XbaI T'CTAG_A Cuts at: 3739 3739 Size: 6403 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2163 3151 3226 2163 Size: 988 75 5340 Fragments arranged by size: 5340 988 75 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6403 XmaI C'CCGG_G Cuts at: 1870 3728 1870 Size: 1858 4545 XmnI GAAnn'nnTTC Cuts at: 2208 4532 2208 Size: 2324 4079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4211 276 Size: 53 83 186 936 2677 2468 Fragments arranged by size: 2677 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI