Restriction map for pFN21AB8655
 (Linear) (Six-base) MAPSORT of: KIBB8655.seq  Check: 7548  from: 1  to: 1698



KIBB8655.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     960    1424    1698 
   Size:        960     464     274

  Fragments arranged by size: 

                 960     464     274
 
AccI GT'mk_AC

Cuts at:      0     242     326    1698 
   Size:        242      84    1372

  Fragments arranged by size: 

                1372     242      84
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     384     985    1698 
   Size:         91     293     601     713

  Fragments arranged by size: 

                 713     601     293      91
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1304    1432    1480    1698 
   Size:       1304     128      48     218

  Fragments arranged by size: 

                1304     218     128      48
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     772     804    1698 
   Size:        772      32     894

  Fragments arranged by size: 

                 894     772      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     157    1200    1698 
   Size:        157    1043     498

  Fragments arranged by size: 

                1043     498     157
 
ApoI r'AATT_y

Cuts at:      0    1014    1048    1698 
   Size:       1014      34     650

  Fragments arranged by size: 

                1014     650      34
 
AvaI C'yCGr_G

Cuts at:      0    1159    1698 
   Size:       1159     539
 
BanII G_rGCy'C

Cuts at:      0    1159    1698 
   Size:       1159     539
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     278     398     949    1698 
   Size:        278     120     551     749

  Fragments arranged by size: 

                 749     551     278     120
 
BbvCI CC'TCA_GC

Cuts at:      0    1652    1698 
   Size:       1652      46
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1293    1327    1698 
   Size:       1293      34     371

  Fragments arranged by size: 

                1293     371      34
 
BlpI GC'TnA_GC

Cuts at:      0    1405    1698 
   Size:       1405     293
 
Bme1580I G_kGCm'C

Cuts at:      0    1360    1698 
   Size:       1360     338
 
BmgBI CAC'GTC

Cuts at:      0      78    1125    1698 
   Size:         78    1047     573

  Fragments arranged by size: 

                1047     573      78
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     568    1698 
   Size:        568    1130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916    1197    1346    1698 
   Size:        916     281     149     352

  Fragments arranged by size: 

                 916     352     281     149
 
Bpu10I CC'TnA_GC

Cuts at:      0    1173    1396    1489    1652    1698 
   Size:       1173     223      93     163      46

  Fragments arranged by size: 

                1173     223     163      93      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1405    1698 
   Size:        208    1197     293

  Fragments arranged by size: 

                1197     293     208
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1528    1698 
   Size:       1528     170
 
BsaAI yAC'GTr

Cuts at:      0     132     714    1698 
   Size:        132     582     984

  Fragments arranged by size: 

                 984     582     132
 
BsaWI w'CCGG_w

Cuts at:      0     761    1698 
   Size:        761     937
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     224     254     772     802    1195    1225    1698 
   Size:        224      30     518      30     393      30     473

  Fragments arranged by size: 

                 518     473     393     224      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     427     499     525     790    1698 
   Size:        427      72      26     265     908

  Fragments arranged by size: 

                 908     427     265      72      26
 
BseYI C'CCAG_C

Cuts at:      0    1277    1698 
   Size:       1277     421
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     783    1093    1597    1698 
   Size:        783     310     504     101

  Fragments arranged by size: 

                 783     504     310     101
 
BsiHKAI G_wGCw'C

Cuts at:      0     839    1698 
   Size:        839     859
 
BsmI GAATG_Cn'

Cuts at:      0     426    1085    1698 
   Size:        426     659     613

  Fragments arranged by size: 

                 659     613     426
 
Bsp1286I G_dGCh'C

Cuts at:      0     839    1159    1360    1698 
   Size:        839     320     201     338

  Fragments arranged by size: 

                 839     338     320     201
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     960    1424    1698 
   Size:        960     464     274

  Fragments arranged by size: 

                 960     464     274
 
BsrDI GCAATG_nn'

Cuts at:      0    1286    1698 
   Size:       1286     412
 
BsrFI r'CCGG_y

Cuts at:      0    1386    1448    1698 
   Size:       1386      62     250

  Fragments arranged by size: 

                1386     250      62
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     504    1698 
   Size:        504    1194
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1125    1698 
   Size:       1125     573
 
BstYI r'GATC_y

Cuts at:      0     101     806    1698 
   Size:        101     705     892

  Fragments arranged by size: 

                 892     705     101
 
BstZ17I GTA'TAC

Cuts at:      0     327    1698 
   Size:        327    1371
 
BtgI C'CryG_G

Cuts at:      0     596     791     992    1239    1514    1698 
   Size:        596     195     201     247     275     184

  Fragments arranged by size: 

                 596     275     247     201     195     184
 
EaeI y'GGCC_r

Cuts at:      0    1115    1698 
   Size:       1115     583
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     404     416     476     537    1028    1698 
   Size:         67     337      12      60      61     491     670

  Fragments arranged by size: 

                 670     491     337      67      61      60      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     817    1698 
   Size:        817     881
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     384     916     985    1197    1346    1698 
   Size:         91     293     532      69     212     149     352

  Fragments arranged by size: 

                 532     352     293     212     149      91      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     967    1698 
   Size:        967     731
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     633    1327    1698 
   Size:         54     579     694     371

  Fragments arranged by size: 

                 694     579     371      54
 
HaeII r_GCGC'y

Cuts at:      0     741    1455    1698 
   Size:        741     714     243

  Fragments arranged by size: 

                 741     714     243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1195    1227    1698 
   Size:       1195      32     471

  Fragments arranged by size: 

                1195     471      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     645    1698 
   Size:        645    1053
 
MscI TGG'CCA

Cuts at:      0    1117    1698 
   Size:       1117     581
 
MslI CAynn'nnrTG

Cuts at:      0     601     703    1069    1698 
   Size:        601     102     366     629

  Fragments arranged by size: 

                 629     601     366     102
 
MspA1I CmG'CkG

Cuts at:      0     519    1266    1295    1588    1698 
   Size:        519     747      29     293     110

  Fragments arranged by size: 

                 747     519     293     110      29
 
NaeI GCC'GGC

Cuts at:      0    1388    1698 
   Size:       1388     310
 
NcoI C'CATG_G

Cuts at:      0     596     992    1239    1698 
   Size:        596     396     247     459

  Fragments arranged by size: 

                 596     459     396     247
 
NgoMIV G'CCGG_C

Cuts at:      0    1386    1698 
   Size:       1386     312
 
NspI r_CATG'y

Cuts at:      0     648    1068    1698 
   Size:        648     420     630

  Fragments arranged by size: 

                 648     630     420
 
PasI CC'CwG_GG

Cuts at:      0    1323    1698 
   Size:       1323     375
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157     993    1200    1245    1698 
   Size:        157     836     207      45     453

  Fragments arranged by size: 

                 836     453     207     157      45
 
PfoI T'CCnGG_A

Cuts at:      0     932    1213    1603    1698 
   Size:        932     281     390      95

  Fragments arranged by size: 

                 932     390     281      95
 
PmlI CAC'GTG

Cuts at:      0     132    1698 
   Size:        132    1566
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     772     804    1698 
   Size:        772      32     894

  Fragments arranged by size: 

                 894     772      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     633    1698 
   Size:         54     579    1065

  Fragments arranged by size: 

                1065     579      54
 
PshAI GACnn'nnGTC

Cuts at:      0     953    1698 
   Size:        953     745
 
PstI C_TGCA'G

Cuts at:      0     737    1698 
   Size:        737     961
 
PvuII CAG'CTG

Cuts at:      0    1266    1295    1698 
   Size:       1266      29     403

  Fragments arranged by size: 

                1266     403      29
 
SapI GCTCTTCn'nnn_

Cuts at:      0     416    1698 
   Size:        416    1282
 
SfcI C'TryA_G

Cuts at:      0     733    1698 
   Size:        733     965
 
SmlI C'TyrA_G

Cuts at:      0     187    1420    1698 
   Size:        187    1233     278

  Fragments arranged by size: 

                1233     278     187
 
StuI AGG'CCT

Cuts at:      0     112    1698 
   Size:        112    1586
 
StyI C'CwwG_G

Cuts at:      0     596     629     992    1239    1698 
   Size:        596      33     363     247     459

  Fragments arranged by size: 

                 596     459     363     247      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     149     313     672    1291    1552    1698 
   Size:        149     164     359     619     261     146

  Fragments arranged by size: 

                 619     359     261     164     149     146
 
TatI w'GTAC_w

Cuts at:      0    1337    1698 
   Size:       1337     361
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      73     105    1698 
   Size:         73      32    1593

  Fragments arranged by size: 

                1593      73      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     588    1126    1698 
   Size:        588     538     572

  Fragments arranged by size: 

                 588     572     538
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     158    1146    1221    1698 
   Size:        158     988      75     477

  Fragments arranged by size: 

                 988     477     158      75
 
XmnI GAAnn'nnTTC

Cuts at:      0     203    1698 
   Size:        203    1495
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI     AloI    AlwNI     ApoI     AvaI
    BanII     BbsI    BbvCI     BcgI     BlpI Bme1580I    BmgBI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
   BstAPI    BstXI    BstYI  BstZ17I     BtgI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI     PstI    PvuII     SapI     SfcI
     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BciVI     BclI    BfrBI     BglI    BglII
     BmrI     BmtI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
     XhoI     XmaI     ZraI
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