Restriction map for
pFN21AB6741
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# NO CUT ENZYMES ( 198 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BlnI BmyI BpmI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 136 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 243 GGTCC G^G[AT]CC
BbsI 288 GAAGAC GAAGAC(2/6)
BclI 155 TGATCA T^GATCA
BlpI 67 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTCAGC GC^T[ACGT]AGC
BpuAI 288 GAAGAC GAAGAC(2/6)
BsmAI 7 GTCTC GTCTC(1/5)
BspHI 35 TCATGA T^CATGA
BspMI 167 GCAGGT ACCTGC(4/8)
BsrI 204 ACTGG ACTGG(1/-1)
Cac8I 26 GCCTGC GC[ACGT]^[ACGT]GC
CelII 67 GCTCAGC GC^T[ACGT]AGC
DdeI 68 CTCAG C^T[ACGT]AG
EarI 144 GAAGAG CTCTTC(1/4)
EcoNI 131 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 155 TGATCA T^GATCA
HaeIII 268 GGCC GG^CC
HapII 270 CCGG C^CGG
HpaII 270 CCGG C^CGG
HphI 158 TCACC GGTGA(8/7)
Ksp632I 144 GAAGAG CTCTTC(1/4)
MaeII 86 ACGT A^CGT
MnlI 138 GAGG CCTC(7/6)
MspI 270 CCGG C^CGG
NlaIII 36 CATG CATG^
NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC
PstI 11 CTGCAG CTGCA^G
RcaI 35 TCATGA T^CATGA
SapI 144 GAAGAGC GCTCTTC(1/4)
SfaNI 76 GATGC GCATC(5/9)
SfcI 11 CTGCAG C^T[GA][CT]AG
Tsp509I 250 AATT ^AATT
TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 229 CCAAAGGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BglII 125 AGATCT A^GATCT
215 AGATCT
BsiYI 131 CCTTGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
161 CCACCAGCAGG
BslI 131 CCTTGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
161 CCACCAGCAGG
BssKI 57 CCAGG ^CC[ACGT]GG
264 CCTGG
BstNI 57 CCAGG CC^[AT]GG
264 CCTGG
BstYI 125 AGATCT [GA]^GATC[CT]
215 AGATCT
Cfr13I 243 GGTCC G^G[ACGT]CC
267 GGGCC
EcoRII 57 CCAGG ^CC[AT]GG
264 CCTGG
MflI 125 AGATCT [GA]^GATC[CT]
215 AGATCT
MvaI 57 CCAGG CC^[AT]GG
264 CCTGG
Sau96I 243 GGTCC G^G[ACGT]CC
267 GGGCC
ScrFI 57 CCAGG CC^[ACGT]GG
264 CCTGG
XhoII 125 AGATCT [GA]^GATC[CT]
215 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BbvI BsaJI BsmFI DpnII
Fnu4HI ItaI MboI MboII NdeII
Sau3AI TseI