(Linear) (Six-base) MAPSORT of: KIBB6737.seq  Check: 7360  from: 1  to: 291



KIBB6737.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     100     291 
   Size:        100     191
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     112     291 
   Size:         33      79     179

  Fragments arranged by size: 

                 179      79      33
 
AleI CACnn'nnGTG

Cuts at:      0      21     291 
   Size:         21     270
 
AlwNI CAG_nnn'CTG

Cuts at:      0      79     291 
   Size:         79     212
 
BanII G_rGCy'C

Cuts at:      0      65     291 
   Size:         65     226
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     291 
   Size:         12     279
 
BseYI C'CCAG_C

Cuts at:      0      67     291 
   Size:         67     224
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     291 
   Size:        262      29
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     291 
   Size:         65     226
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     100     291 
   Size:        100     191
 
EaeI y'GGCC_r

Cuts at:      0     107     203     291 
   Size:        107      96      88

  Fragments arranged by size: 

                 107      96      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     112     291 
   Size:         33      79     179

  Fragments arranged by size: 

                 179      79      33
 
EcoRV GAT'ATC

Cuts at:      0     213     291 
   Size:        213      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     291 
   Size:        256      32       3

  Fragments arranged by size: 

                 256      32       3
 
MscI TGG'CCA

Cuts at:      0     109     205     291 
   Size:        109      96      86

  Fragments arranged by size: 

                 109      96      86
 
MslI CAynn'nnrTG

Cuts at:      0      21     291 
   Size:         21     270
 
MspA1I CmG'CkG

Cuts at:      0      43     291 
   Size:         43     248
 
PasI CC'CwG_GG

Cuts at:      0      57     291 
   Size:         57     234
 
PvuII CAG'CTG

Cuts at:      0      43     291 
   Size:         43     248
 
SfcI C'TryA_G

Cuts at:      0     144     291 
   Size:        144     147
 
StyI C'CwwG_G

Cuts at:      0     206     291 
   Size:        206      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     228     291 
   Size:        228      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      64     258     291 
   Size:         64     194      33

  Fragments arranged by size: 

                 194      64      33
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI    BanII     BsaI    BseYI     BsgI
 Bsp1286I    BspMI     EaeI  Eco57MI    EcoRV    Hin4I     MscI     MslI
   MspA1I     PasI    PvuII     SfcI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI