Restriction map for
pFN21AE0219
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmFI BsmI Bsp1286I
BspDI BspEI BspHI BspLU11I BsrBI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 99 CGCG CG^CG
AcsI 302 GAATTC [GA]^AATT[CT]
ApoI 302 GAATTC [GA]^AATT[CT]
BalI 275 TGGCCA TGG^CCA
BamHI 120 GGATCC G^GATCC
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
BbvI 215 GCAGC GCAGC(8/12)
BcnI 117 CCGGG CC^[GC]GG
BpmI 7 CTGGAG CTGGAG(16/14)
BsaAI 244 TACGTA [CT]AC^GT[GA]
BsaJI 284 CCCTGG C^C[ACGT][ACGT]GG
BsiYI 111 CCAGGACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 111 CCAGGACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 10 GAGAC GTCTC(1/5)
BsmBI 10 GAGACG CGTCTC(1/5)
Bsp1407I 251 TGTACA T^GTACA
BspMI 361 ACCTGC ACCTGC(4/8)
BsrGI 251 TGTACA T^GTACA
BstF5I 162 GGATG GGATG(2/0)
BstUI 99 CGCG CG^CG
BstXI 278 CCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 467 GCCTGC GC[ACGT]^[ACGT]GC
EaeI 275 TGGCCA [CT]^GGCC[GA]
EcoRI 302 GAATTC G^AATTC
FauI 298 GCGGG CCCGC(4/6)
FokI 162 GGATG GGATG(9/13)
HphI 180 GGTGA GGTGA(8/7)
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
MseI 343 TTAA T^TAA
MvnI 99 CGCG CG^CG
NciI 117 CCGGG CC^[GC]GG
NlaIV 120 GGATCC GG[ACGT]^[ACGT]CC
PaeR7I 73 CTCGAG C^TCGAG
PstI 213 CTGCAG CTGCA^G
PvuII 321 CAGCTG CAG^CTG
SapI 33 GCTCTTC GCTCTTC(1/4)
SnaBI 244 TACGTA TAC^GTA
SspBI 251 TGTACA T^GTACA
Tru9I 343 TTAA T^TAA
TseI 215 GCAGC G^C[AT]GC
Tsp509I 303 AATT ^AATT
TspRI 383 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 73 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaI 73 CTCGAG C^[CT]CG[GA]G
88 CCCGAG
AvaII 108 GGACC G^G[AT]CC
114 GGACC
BsgI 328 CTGCAC GTGCAG(16/14)
385 GTGCAG
BsoBI 73 CTCGAG C^[CT]CG[GA]G
88 CCCGAG
BsrDI 294 CATTGC GCAATG(2/0)
420 CATTGC
BsrFI 128 ACCGGC [GA]^CCGG[CT]
177 GCCGGT
BstYI 120 GGATCC [GA]^GATC[CT]
209 GGATCT
Cfr10I 128 ACCGGC [GA]^CCGG[CT]
177 GCCGGT
Cfr13I 108 GGACC G^G[ACGT]CC
114 GGACC
DdeI 319 CTCAG C^T[ACGT]AG
475 CTGAG
Fnu4HI 68 GCGGC GC^[ACGT]GC
215 GCAGC
HinfI 49 GACTC G^A[ACGT]TC
103 GACTC
ItaI 68 GCGGC GC^[ACGT]GC
215 GCAGC
MaeII 26 ACGT A^CGT
245 ACGT
MaeIII 181 GTGAC ^GT[ACGT]AC
406 GTGAC
MflI 120 GGATCC [GA]^GATC[CT]
209 GGATCT
MspA1I 66 CAGCGG C[AC]G^C[GT]G
321 CAGCTG
NlaIII 39 CATG CATG^
220 CATG
PleI 49 GACTC GAGTC(4/5)
103 GACTC
Sau96I 108 GGACC G^G[ACGT]CC
114 GGACC
SfaNI 449 GATGC GCATC(5/9)
141 GCATC
SfcI 54 CTACAG C^T[GA][CT]AG
213 CTGCAG
SmlI 73 CTCGAG C^T[CT][GA]AG
145 CTCAAG
TaqI 74 TCGA T^CGA
158 TCGA
Tsp45I 181 GTGAC ^GT[GC]AC
406 GTGAC
TthHB8I 74 TCGA T^CGA
158 TCGA
XhoII 120 GGATCC [GA]^GATC[CT]
209 GGATCT
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI AluI AlwI BssKI
BstNI DpnII EarI EcoRII HaeIII
HapII HpaII Ksp632I MboI MboII
MnlI MspI MvaI MwoI NdeII
RsaI Sau3AI ScrFI