Restriction map for pFN21AE0218
 (Circular) (Six-base) MAPSORT of: pFN21AE0218.seq  Check: 5793  from: 1  to: 5239



pFN21AE0218  5239 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3049     278 
   Size:         53      83     186     936    1513    2468

  Fragments arranged by size: 

                2468    1513     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2025    2582    1937 
   Size:         88     557    4594

  Fragments arranged by size: 

                4594     557      88
 
Acc65I G'GTAC_C

Cuts at:   2560    2560 
   Size:       5239
 
AclI AA'CG_TT

Cuts at:   1563    3366    3739    1563 
   Size:       1803     373    3063

  Fragments arranged by size: 

                3063    1803     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3302    4686    4954    1632 
   Size:       1670    1384     268    1917

  Fragments arranged by size: 

                1917    1670    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5042
 
AflIII A'CryG_T

Cuts at:   1203    4138    5115    1203 
   Size:       2935     977    1327

  Fragments arranged by size: 

                2935    1327     977
 
AgeI A'CCGG_T

Cuts at:   4070    4070 
   Size:       5239
 
AhdI GACnn_n'nnGTC

Cuts at:   3968    3968 
   Size:       5239
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5239
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2540    1184 
   Size:       1356    3883
 
AlwNI CAG_nnn'CTG

Cuts at:   2982    4554    2982 
   Size:       1572    3667
 
ApaLI G'TGCA_C

Cuts at:   3296    4452    3296 
   Size:       1156    4083
 
ApoI r'AATT_y

Cuts at:   1493    2548    2774    2926    5035    1493 
   Size:       1055     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1055     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3793     160 
   Size:        872    2761    1606

  Fragments arranged by size: 

                2761    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5239
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2564    1870 
   Size:         73     621    4545

  Fragments arranged by size: 

                4545     621      73
 
BamHI G'GATC_C

Cuts at:   2569    2936    2569 
   Size:        367    4872
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2560    4015     618 
   Size:        325     217     627     773    1455    1842

  Fragments arranged by size: 

                1842    1455     773     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2558    1391 
   Size:       1167    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4268
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2341    2375    3430    3464    1903 
   Size:         34     404      34    1055      34    3678

  Fragments arranged by size: 

                3678    1055     404      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3131    4341    3131 
   Size:       1210    4029
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5239
 
BfrBI ATG'CAT

Cuts at:   2403    2403 
   Size:       5239
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3850     136 
   Size:        107     122      71     631     605    2178    1525

  Fragments arranged by size: 

                2178    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5234    5234 
   Size:       5239
 
BlpI GC'TnA_GC

Cuts at:   2111    2656    2111 
   Size:        545    4694
 
Bme1580I G_kGCm'C

Cuts at:   1790    3300    4456    1790 
   Size:       1510    1156    2573

  Fragments arranged by size: 

                2573    1510    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2191    1318 
   Size:        873    4366
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2970    3928     442 
   Size:        416      53      83     399      24    1553     958    1753

  Fragments arranged by size: 

                1753    1553     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3899    1239 
   Size:       2660    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3364    4229    4527    3364 
   Size:        865     298    4076

  Fragments arranged by size: 

                4076     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3902     882 
   Size:        617    2403    2219

  Fragments arranged by size: 

                2403    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2023    3030     493 
   Size:        711     819    1007    2702

  Fragments arranged by size: 

                2702    1007     819     711
 
BsaBI GATnn'nnATC

Cuts at:   5233    5233 
   Size:       5239
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3046    3428    4063     275 
   Size:         53      83     186     936    1513     382     635    1451

  Fragments arranged by size: 

                1513    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3672    4070    4344    4491    4902    3672 
   Size:        398     274     147     411    4009

  Fragments arranged by size: 

                4009     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5014

  Fragments arranged by size: 

                5014     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5239
 
BseYI C'CCAG_C

Cuts at:   4442    4938    4442 
   Size:        496    4743
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2363    1163 
   Size:        573     627    4039

  Fragments arranged by size: 

                4039     627     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2689    3450    4478    1540 
   Size:        275     186     688     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     688     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3300    3385    4456    3300 
   Size:         85    1071    4083

  Fragments arranged by size: 

                4083    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2750    2843    2750 
   Size:         93    5146
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2558    3300    3385    4456    1391 
   Size:        399     768     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     768     742     399      85
 
BspEI T'CCGG_A

Cuts at:   4902    4902 
   Size:       5239
 
BspHI T'CATG_A

Cuts at:   3128    4858    3128 
   Size:       1730    3509
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2594     844 
   Size:       1750    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3126    1394 
   Size:        429    1303    3507

  Fragments arranged by size: 

                3507    1303     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2515    3734    3908      65 
   Size:       1183    1267    1219     174    1396

  Fragments arranged by size: 

                1396    1267    1219    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3883    4070    1700 
   Size:        110    2073     187    2869

  Fragments arranged by size: 

                2869    2073     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5239
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5239
 
BssSI C'ACGA_G

Cuts at:   3299    4311    3299 
   Size:       1012    4227
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5239
 
BstBI TT'CG_AA

Cuts at:   4043    4043 
   Size:       5239
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5239
 
BstYI r'GATC_y

Cuts at:   1972    2569    2936    3321    3338    4790    4876    4888    5234 
   Size:        597     367     385      17    1452      86      12     346

Cuts at:   5234    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     597     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2097     513 
   Size:        552    1032    3655

  Fragments arranged by size: 

                3655    1032     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4500

  Fragments arranged by size: 

                4500     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2755    3549    3569    2755 
   Size:        794      20    4425

  Fragments arranged by size: 

                4425     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5239
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5204
 
DraI TTT'AAA

Cuts at:   2543    2543 
   Size:       5239
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4246     809 
   Size:        507    2930    1802

  Fragments arranged by size: 

                2930    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2686    3575    5204 
   Size:         54    1425      50     275      18     856     889    1629

Cuts at:   5204       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     856     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2686    1537 
   Size:        275     874    4090

  Fragments arranged by size: 

                4090     874     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3169    1353 
   Size:       1816    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3826    4200    4346     387 
   Size:       1064     138    2237     374     146    1280

  Fragments arranged by size: 

                2237    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3302    3899    4686    4954    1239 
   Size:        393    1670     597     787     268    1524

  Fragments arranged by size: 

                1670    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2548    2926    2548 
   Size:        378    4861
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2221    2284    2221 
   Size:         63    5176
 
FspI TGC'GCA

Cuts at:   3745    3745 
   Size:       5239
 
HaeII r_GCGC'y

Cuts at:   1678    4386    1678 
   Size:       2708    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3880    3912    3954    3986    1373 
   Size:         32     135      32    2308      32      42      32    2626

  Fragments arranged by size: 

                2626    2308     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2161    2583    2829     669 
   Size:        988     281     223     422     246    3079

  Fragments arranged by size: 

                3079     988     422     281     246     223
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5239
 
HpaI GTT'AAC

Cuts at:   2829    2829 
   Size:       5239
 
KpnI G_GTAC'C

Cuts at:   2564    2564 
   Size:       5239
 
MfeI C'AATT_G

Cuts at:   2838    2838 
   Size:       5239
 
MluI A'CGCG_T

Cuts at:   5115    5115 
   Size:       5239
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4353    4537    1305 
   Size:       3048     184    2007

  Fragments arranged by size: 

                3048    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3417

  Fragments arranged by size: 

                3417    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2333    3197    3556    3715     518 
   Size:        648    1167     864     359     159    2042

  Fragments arranged by size: 

                2042    1167     864     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2655    3332    4480    4725    1606 
   Size:       1049     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1049     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2097     513 
   Size:        552    1032    3655

  Fragments arranged by size: 

                3655    1032     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5239
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5239
 
NotI GC'GGCC_GC

Cuts at:   2686    2686 
   Size:       5239
 
NsiI A_TGCA'T

Cuts at:   2405    2405 
   Size:       5239
 
NspI r_CATG'y

Cuts at:   1132    1229    2597    4142    1132 
   Size:         97    1368    1545    2229

  Fragments arranged by size: 

                2229    1545    1368      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5239
 
PciI A'CATG_T

Cuts at:   4138    4138 
   Size:       5239
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5239
 
PfoI T'CCnGG_A

Cuts at:   2058    2058 
   Size:       5239
 
PmeI GTTT'AAAC

Cuts at:   2543    2543 
   Size:       5239
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3308    3340    4821    4853    3308 
   Size:         32    1481      32    3694

  Fragments arranged by size: 

                3694    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5239
 
PsiI TTA'TAA

Cuts at:   2809    2809 
   Size:       5239
 
PstI C_TGCA'G

Cuts at:    830    2591     830 
   Size:       1761    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5239
 
SalI G'TCGA_C

Cuts at:   1936    2581    1936 
   Size:        645    4594
 
SbfI CC_TGCA'GG

Cuts at:   2591    2591 
   Size:       5239
 
ScaI AGT'ACT

Cuts at:   3487    5199    3487 
   Size:       1712    3527
 
SexAI A'CCwGG_T

Cuts at:   2154    2154 
   Size:       5239
 
SfcI C'TryA_G

Cuts at:    826    1045    2587    3722    4403    4594     826 
   Size:        219    1542    1135     681     191    1471

  Fragments arranged by size: 

                1542    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5239
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5239
 
SmaI CCC'GGG

Cuts at:   1872    2566    1872 
   Size:        694    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3343    4244    4506     820 
   Size:        197     926    1400     901     262    1553

  Fragments arranged by size: 

                1553    1400     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2023    3030     493 
   Size:       1530    1007    2702

  Fragments arranged by size: 

                2702    1530    1007
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5239
 
SphI G_CATG'C

Cuts at:   1132    2597    1132 
   Size:       1465    3774
 
SspI AAT'ATT

Cuts at:      5      52    2502    3163       5 
   Size:         47    2450     661    2081

  Fragments arranged by size: 

                2450    2081     661      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5239
 
StyI C'CwwG_G

Cuts at:    513    1065    2097    2142    2678     513 
   Size:        552    1032      45     536    3074

  Fragments arranged by size: 

                3074    1032     552     536      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3266    3283    3436    3621    4066     325 
   Size:       1255    1686      17     153     185     445    1498

  Fragments arranged by size: 

                1686    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3485    5197      96 
   Size:        274      80      33      51    1443    1508    1712     138

  Fragments arranged by size: 

                1712    1508    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5199    5231    5199 
   Size:         32    5207
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2156    2192    1316 
   Size:        840      36    4363

  Fragments arranged by size: 

                4363     840      36
 
XbaI T'CTAG_A

Cuts at:   2575    2575 
   Size:       5239
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5239
 
XmaI C'CCGG_G

Cuts at:   1870    2564    1870 
   Size:        694    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2501    3368    2501 
   Size:        867    4372
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3047     276 
   Size:         53      83     186     936    1513    2468

  Fragments arranged by size: 

                2468    1513     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     PacI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SfiI     SfoI     SrfI     SwaI     XcmI
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