(Linear) (Six-base) MAPSORT of: KIBB3164.seq Check: 1128 from: 1 to: 2763 KIBB3164.orf With 182 enzymes: SgfI * March 2, 2010 14:25 .. AatII G_ACGT'C Cuts at: 0 605 2763 Size: 605 2158 AccI GT'mk_AC Cuts at: 0 599 2763 Size: 599 2164 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 995 1306 2014 2530 2584 2763 Size: 51 944 311 708 516 54 179 Fragments arranged by size: 944 708 516 311 179 54 51 AflII C'TTAA_G Cuts at: 0 826 2763 Size: 826 1937 AflIII A'CryG_T Cuts at: 0 534 2763 Size: 534 2229 AgeI A'CCGG_T Cuts at: 0 2279 2763 Size: 2279 484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 128 787 2763 Size: 128 659 1976 Fragments arranged by size: 1976 659 128 AlwNI CAG_nnn'CTG Cuts at: 0 194 375 1050 2475 2508 2607 2763 Size: 194 181 675 1425 33 99 156 Fragments arranged by size: 1425 675 194 181 156 99 33 ApaLI G'TGCA_C Cuts at: 0 2724 2763 Size: 2724 39 ApoI r'AATT_y Cuts at: 0 274 889 946 1244 1604 2763 Size: 274 615 57 298 360 1159 Fragments arranged by size: 1159 615 360 298 274 57 BamHI G'GATC_C Cuts at: 0 777 966 2763 Size: 777 189 1797 Fragments arranged by size: 1797 777 189 BanI G'GyrC_C Cuts at: 0 465 558 2763 Size: 465 93 2205 Fragments arranged by size: 2205 465 93 BanII G_rGCy'C Cuts at: 0 2720 2763 Size: 2720 43 BbsI GAAGACnn'nnnn_ Cuts at: 0 1004 1172 1715 2071 2247 2763 Size: 1004 168 543 356 176 516 Fragments arranged by size: 1004 543 516 356 176 168 BbvCI CC'TCA_GC Cuts at: 0 883 2763 Size: 883 1880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2030 2064 2763 Size: 2030 34 699 Fragments arranged by size: 2030 699 34 BclI T'GATC_A Cuts at: 0 1956 2763 Size: 1956 807 BfrBI ATG'CAT Cuts at: 0 1557 2763 Size: 1557 1206 BglII A'GATC_T Cuts at: 0 171 291 1152 1841 2154 2763 Size: 171 120 861 689 313 609 Fragments arranged by size: 861 689 609 313 171 120 Bme1580I G_kGCm'C Cuts at: 0 2728 2763 Size: 2728 35 BmgBI CAC'GTC Cuts at: 0 15 535 2493 2763 Size: 15 520 1958 270 Fragments arranged by size: 1958 520 270 15 BmrI ACTGGGnnnn_n' Cuts at: 0 110 2763 Size: 110 2653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 548 1400 2464 2558 2722 2763 Size: 548 852 1064 94 164 41 Fragments arranged by size: 1064 852 548 164 94 41 Bpu10I CC'TnA_GC Cuts at: 0 883 1666 2763 Size: 883 783 1097 Fragments arranged by size: 1097 883 783 BsaI GGTCTCn'nnnn_ Cuts at: 0 2014 2440 2763 Size: 2014 426 323 Fragments arranged by size: 2014 426 323 BsaBI GATnn'nnATC Cuts at: 0 1133 2763 Size: 1133 1630 BsaHI Gr'CG_yC Cuts at: 0 602 2763 Size: 602 2161 BsaWI w'CCGG_w Cuts at: 0 2279 2453 2763 Size: 2279 174 310 Fragments arranged by size: 2279 310 174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 521 551 991 1021 1368 1398 2298 2328 Size: 521 30 440 30 347 30 900 30 Cuts at: 2328 2556 2586 2763 Size: 228 30 177 Fragments arranged by size: 900 521 440 347 228 177 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 999 1373 2763 Size: 999 374 1390 Fragments arranged by size: 1390 999 374 BseYI C'CCAG_C Cuts at: 0 1767 2763 Size: 1767 996 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 938 2543 2763 Size: 938 1605 220 Fragments arranged by size: 1605 938 220 BsiHKAI G_wGCw'C Cuts at: 0 1857 2720 2728 2763 Size: 1857 863 8 35 Fragments arranged by size: 1857 863 35 8 BsmI GAATG_Cn' Cuts at: 0 260 1910 2191 2763 Size: 260 1650 281 572 Fragments arranged by size: 1650 572 281 260 Bsp1286I G_dGCh'C Cuts at: 0 1857 2720 2728 2763 Size: 1857 863 8 35 Fragments arranged by size: 1857 863 35 8 BspEI T'CCGG_A Cuts at: 0 2453 2763 Size: 2453 310 BspHI T'CATG_A Cuts at: 0 2361 2763 Size: 2361 402 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1996 2763 Size: 1996 767 BsrBI CCG'CTC Cuts at: 0 834 2763 Size: 834 1929 BsrDI GCAATG_nn' Cuts at: 0 2051 2552 2763 Size: 2051 501 211 Fragments arranged by size: 2051 501 211 BsrFI r'CCGG_y Cuts at: 0 2279 2763 Size: 2279 484 BsrGI T'GTAC_A Cuts at: 0 335 2763 Size: 335 2428 BssSI C'ACGA_G Cuts at: 0 2416 2464 2719 2763 Size: 2416 48 255 44 Fragments arranged by size: 2416 255 48 44 BstAPI GCAn_nnn'nTGC Cuts at: 0 1662 2533 2763 Size: 1662 871 230 Fragments arranged by size: 1662 871 230 BstBI TT'CG_AA Cuts at: 0 272 2763 Size: 272 2491 BstXI CCAn_nnnn'nTGG Cuts at: 0 722 2763 Size: 722 2041 BstYI r'GATC_y Cuts at: 0 171 291 777 966 1152 1841 2154 2459 Size: 171 120 486 189 186 689 313 305 Cuts at: 2459 2763 Size: 304 Fragments arranged by size: 689 486 313 305 304 189 186 171 120 Bsu36I CC'TnA_GG Cuts at: 0 1011 2501 2763 Size: 1011 1490 262 Fragments arranged by size: 1490 1011 262 BtgI C'CryG_G Cuts at: 0 820 2057 2763 Size: 820 1237 706 Fragments arranged by size: 1237 820 706 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1223 2440 2763 Size: 1223 1217 323 Fragments arranged by size: 1223 1217 323 BtsI GCAGTG_nn' Cuts at: 0 727 1095 2763 Size: 727 368 1668 Fragments arranged by size: 1668 727 368 DraI TTT'AAA Cuts at: 0 1403 1514 2582 2763 Size: 1403 111 1068 181 Fragments arranged by size: 1403 1068 181 111 DraIII CAC_nnn'GTG Cuts at: 0 2230 2763 Size: 2230 533 EaeI y'GGCC_r Cuts at: 0 2 2763 Size: 2 2761 EarI CTCTTCn'nnn_ Cuts at: 0 971 1058 2603 2763 Size: 971 87 1545 160 Fragments arranged by size: 1545 971 160 87 EcoICRI GAG'CTC Cuts at: 0 2718 2763 Size: 2718 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 548 995 1306 1400 2014 2464 2530 Size: 51 497 447 311 94 614 450 66 Cuts at: 2530 2558 2584 2722 2763 Size: 28 26 138 41 Fragments arranged by size: 614 497 450 447 311 138 94 66 51 41 28 26 EcoO109I rG'GnC_Cy Cuts at: 0 61 1098 2514 2742 2763 Size: 61 1037 1416 228 21 Fragments arranged by size: 1416 1037 228 61 21 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 81 599 1603 2763 Size: 81 518 1004 1160 Fragments arranged by size: 1160 1004 518 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 71 103 762 794 991 1023 1798 1826 Size: 71 32 659 32 197 32 775 28 Cuts at: 1826 1830 1858 2578 2610 2763 Size: 4 28 720 32 153 Fragments arranged by size: 775 720 659 197 153 71 32 32 32 32 28 28 4 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 519 2763 Size: 519 2244 MslI CAynn'nnrTG Cuts at: 0 419 769 1441 1595 1627 2056 2420 2763 Size: 419 350 672 154 32 429 364 343 Fragments arranged by size: 672 429 419 364 350 343 154 32 MspA1I CmG'CkG Cuts at: 0 784 882 931 2090 2763 Size: 784 98 49 1159 673 Fragments arranged by size: 1159 784 673 98 49 NcoI C'CATG_G Cuts at: 0 820 2057 2763 Size: 820 1237 706 Fragments arranged by size: 1237 820 706 NdeI CA'TA_TG Cuts at: 0 200 2166 2640 2763 Size: 200 1966 474 123 Fragments arranged by size: 1966 474 200 123 NsiI A_TGCA'T Cuts at: 0 1559 2763 Size: 1559 1204 NspI r_CATG'y Cuts at: 0 768 2763 Size: 768 1995 PflMI CCAn_nnn'nTGG Cuts at: 0 1644 1774 2763 Size: 1644 130 989 Fragments arranged by size: 1644 989 130 PfoI T'CCnGG_A Cuts at: 0 1124 2763 Size: 1124 1639 PpuMI rG'GwC_Cy Cuts at: 0 61 1098 2514 2742 2763 Size: 61 1037 1416 228 21 Fragments arranged by size: 1416 1037 228 61 21 PstI C_TGCA'G Cuts at: 0 1090 2007 2529 2612 2763 Size: 1090 917 522 83 151 Fragments arranged by size: 1090 917 522 151 83 PvuII CAG'CTG Cuts at: 0 784 882 2090 2763 Size: 784 98 1208 673 Fragments arranged by size: 1208 784 673 98 SacI G_AGCT'C Cuts at: 0 2720 2763 Size: 2720 43 SanDI GG'GwC_CC Cuts at: 0 61 2742 2763 Size: 61 2681 21 Fragments arranged by size: 2681 61 21 ScaI AGT'ACT Cuts at: 0 923 2398 2763 Size: 923 1475 365 Fragments arranged by size: 1475 923 365 SfcI C'TryA_G Cuts at: 0 347 596 1086 2003 2525 2608 2763 Size: 347 249 490 917 522 83 155 Fragments arranged by size: 917 522 490 347 249 155 83 SmlI C'TyrA_G Cuts at: 0 826 2763 Size: 826 1937 SpeI A'CTAG_T Cuts at: 0 2208 2763 Size: 2208 555 SphI G_CATG'C Cuts at: 0 768 2763 Size: 768 1995 SspI AAT'ATT Cuts at: 0 2134 2763 Size: 2134 629 StyI C'CwwG_G Cuts at: 0 665 715 820 1877 2057 2763 Size: 665 50 105 1057 180 706 Fragments arranged by size: 1057 706 665 180 105 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 833 2288 2763 Size: 833 1455 475 Fragments arranged by size: 1455 833 475 TatI w'GTAC_w Cuts at: 0 163 335 921 1215 2092 2120 2396 2763 Size: 163 172 586 294 877 28 276 367 Fragments arranged by size: 877 586 367 294 276 172 163 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 135 167 1368 1400 2498 2530 2726 2758 Size: 135 32 1201 32 1098 32 196 32 Cuts at: 2758 2763 Size: 5 Fragments arranged by size: 1201 1098 196 135 32 32 32 32 5 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1233 1464 2763 Size: 1233 231 1299 Fragments arranged by size: 1299 1233 231 XmnI GAAnn'nnTTC Cuts at: 0 1067 2597 2763 Size: 1067 1530 166 Fragments arranged by size: 1530 1067 166 ZraI GAC'GTC Cuts at: 0 603 2763 Size: 603 2160 Enzymes that do cut: AatII AccI AcuI AflII AflIII AgeI AlfI AlwNI ApaLI ApoI BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MmeI MslI MspA1I NcoI NdeI NsiI NspI PflMI PfoI PpuMI PstI PvuII SacI SanDI ScaI SfcI SmlI SpeI SphI SspI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BbeI BciVI BglI BlpI BmtI BplI BpuEI BsaAI BsiEI BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI Tth111I XbaI XhoI XmaI