################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccII 1633 CGCG CG^CG AflIII 2665 ACATGT A^C[GA][CT]GT AseI 1945 ATTAAT AT^TAAT AsnI 1945 ATTAAT AT^TAAT AspHI 2587 GAGCTC G[AT]GC[AT]^C AvaI 2297 CTCGAG C^[CT]CG[GA]G BalI 2 TGGCCA TGG^CCA BamHI 1008 GGATCC G^GATCC BanII 2587 GAGCTC G[GA]GC[CT]^C BclI 1545 TGATCA T^GATCA BcnI 1715 CCCGG CC^[GC]GG BglI 535 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2584 GCTGAGC GC^T[ACGT]AGC BmyI 2587 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 2584 GCTGAGC GC^T[ACGT]AGC BsaI 1134 GAGACC GGTCTC(1/5) BsgI 899 GTGCAG GTGCAG(16/14) BsiHKAI 2587 GAGCTC G[AT]GC[AT]^C BsmBI 650 CGTCTC CGTCTC(1/5) BsmFI 1713 GTCCC GGGAC(10/14) BsoBI 2297 CTCGAG C^[CT]CG[GA]G Bsp1286I 2587 GAGCTC G[AGT]GC[ACT]^C BspDI 2020 ATCGAT AT^CGAT BspLU11I 2665 ACATGT A^CATGT BspMI 2557 ACCTGC ACCTGC(4/8) BsrDI 1583 GCAATG GCAATG(2/0) BssSI 1520 CTCGTG CACGAG(-5/-1) BstEII 2648 GGTTACC G^GT[ACGT]ACC BstPI 2648 GGTTACC G^GT[ACGT]ACC BstUI 1633 CGCG CG^CG CelII 2584 GCTGAGC GC^T[ACGT]AGC CfoI 1782 GCGC GCG^C ClaI 2020 ATCGAT AT^CGAT DraI 261 TTTAAA TTT^AAA EaeI 2 TGGCCA [CT]^GGCC[GA] EcoNI 2569 CCTCTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2648 GGTTACC G^GT[ACGT]ACC FauI 1900 GCGGG CCCGC(4/6) FbaI 1545 TGATCA T^GATCA HaeII 1781 GGCGCT [GA]GCGC^[CT] HapII 1716 CCGG C^CGG HgaI 763 GCGTC GACGC(5/10) HhaI 1782 GCGC GCG^C HinP1I 1782 GCGC G^CGC HpaII 1716 CCGG C^CGG MaeII 1222 ACGT A^CGT MfeI 1285 CAATTG C^AATTG MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspI 1716 CCGG C^CGG MunI 1285 CAATTG C^AATTG MvnI 1633 CGCG CG^CG NciI 1715 CCCGG CC^[GC]GG PaeR7I 2297 CTCGAG C^TCGAG PshBI 1945 ATTAAT AT^TAAT SacI 2587 GAGCTC GAGCT^C SphI 2408 GCATGC GCATG^C XbaI 952 TCTAGA T^CTAGA XcmI 896 CCAGTGCAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2297 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 1366 GGACC G^G[AT]CC 1612 GGTCC BsmI 1032 GAATGC GAATGC(1/-1) 2528 GAATGC BstF5I 737 CATCC GGATG(2/0) 192 GGATG DsaI 1238 CCATGG C^C[GA][CT]GG 1994 CCATGG FokI 737 CATCC GGATG(9/13) 192 GGATG HincII 2176 GTTGAC GT[CT]^[GA]AC 2419 GTTGAC HindII 2176 GTTGAC GT[CT]^[GA]AC 2419 GTTGAC HindIII 1648 AAGCTT A^AGCTT 2321 AAGCTT MslI 502 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1055 CATCAGGGTG MspA1I 1228 CAGCGG C[AC]G^C[GT]G 1276 CAGCGG NcoI 1238 CCATGG C^CATGG 1994 CCATGG NspI 2408 GCATGC [GA]CATG^[CT] 2665 ACATGT ScaI 823 AGTACT AGT^ACT 2369 AGTACT SpeI 28 ACTAGT A^CTAGT 820 ACTAGT SspI 1794 AATATT AAT^ATT 2233 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I BbvI BfaI BpmI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI