(Circular) (Six-base) MAPSORT of: pFN21AB8007.seq Check: 567 from: 1 to: 7471 pFN21AB8007 7471 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5281 278 Size: 53 83 186 936 3745 2468 Fragments arranged by size: 3745 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4814 1937 Size: 2877 4594 Acc65I G'GTAC_C Cuts at: 4792 4792 Size: 7471 AclI AA'CG_TT Cuts at: 1563 5598 5971 1563 Size: 4035 373 3063 Fragments arranged by size: 4035 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 2817 2940 4241 4346 5534 6918 7186 Size: 876 309 123 1301 105 1188 1384 268 Cuts at: 7186 1632 Size: 1917 Fragments arranged by size: 1917 1384 1301 1188 876 309 268 123 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7274 AflIII A'CryG_T Cuts at: 1203 4670 6370 7347 1203 Size: 3467 1700 977 1327 Fragments arranged by size: 3467 1700 1327 977 AgeI A'CCGG_T Cuts at: 6302 6302 Size: 7471 AhdI GACnn_n'nnGTC Cuts at: 6200 6200 Size: 7471 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7471 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7471 AlwNI CAG_nnn'CTG Cuts at: 5214 6786 5214 Size: 1572 5899 ApaLI G'TGCA_C Cuts at: 5528 6684 5528 Size: 1156 6315 ApoI r'AATT_y Cuts at: 1493 2240 3259 4109 4780 5006 5158 7267 1493 Size: 747 1019 850 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 850 747 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 3951 6025 160 Size: 872 2919 2074 1606 Fragments arranged by size: 2919 2074 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7471 AvaI C'yCGr_G Cuts at: 1870 1943 4302 4796 1870 Size: 73 2359 494 4545 Fragments arranged by size: 4545 2359 494 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2772 2805 2772 Size: 33 7438 BamHI G'GATC_C Cuts at: 3013 4801 5168 3013 Size: 1788 367 5316 Fragments arranged by size: 5316 1788 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4792 6247 618 Size: 325 217 627 3005 1455 1842 Fragments arranged by size: 3005 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4596 4790 1391 Size: 3205 194 4072 Fragments arranged by size: 4072 3205 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6500 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5662 5696 1903 Size: 34 3725 34 3678 Fragments arranged by size: 3725 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5363 6573 5363 Size: 1210 6261 BclI T'GATC_A Cuts at: 1548 3550 1548 Size: 2002 5469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 6082 136 Size: 107 122 71 631 605 874 3536 1525 Fragments arranged by size: 3536 1525 874 631 605 122 107 71 BglII A'GATC_T Cuts at: 7466 7466 Size: 7471 BlpI GC'TnA_GC Cuts at: 4590 4888 4590 Size: 298 7173 Bme1580I G_kGCm'C Cuts at: 1790 5532 6688 1790 Size: 3742 1156 2573 Fragments arranged by size: 3742 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7471 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3445 5202 6160 Size: 416 53 83 399 24 2028 1757 958 Cuts at: 6160 442 Size: 1753 Fragments arranged by size: 2028 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7471 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2769 2832 3141 6131 1239 Size: 1530 63 309 2990 2579 Fragments arranged by size: 2990 2579 1530 309 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3456 3799 5596 6461 6759 3456 Size: 343 1797 865 298 4168 Fragments arranged by size: 4168 1797 865 343 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3133 6134 882 Size: 617 1634 3001 2219 Fragments arranged by size: 3001 2219 1634 617 BsaAI yAC'GTr Cuts at: 493 1204 5262 493 Size: 711 4058 2702 Fragments arranged by size: 4058 2702 711 BsaBI GATnn'nnATC Cuts at: 7465 7465 Size: 7471 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5278 5660 6295 275 Size: 53 83 186 936 3745 382 635 1451 Fragments arranged by size: 3745 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5904 6302 6576 6723 7134 5904 Size: 398 274 147 411 6241 Fragments arranged by size: 6241 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2674 2704 2767 2797 1178 Size: 30 165 30 1271 30 63 30 5852 Fragments arranged by size: 5852 1271 165 63 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3796 3822 4268 4320 1186 Size: 2610 26 446 52 4337 Fragments arranged by size: 4337 2610 446 52 26 BseYI C'CCAG_C Cuts at: 3387 3507 6674 7170 3387 Size: 120 3167 496 3688 Fragments arranged by size: 3688 3167 496 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2925 1163 Size: 573 1189 5709 Fragments arranged by size: 5709 1189 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4921 5682 6710 1540 Size: 275 186 2920 761 1028 2301 Fragments arranged by size: 2920 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4596 5532 5617 6688 4596 Size: 936 85 1071 5379 Fragments arranged by size: 5379 1071 936 85 BsmI GAATG_Cn' Cuts at: 3043 4539 4982 5075 3043 Size: 1496 443 93 5439 Fragments arranged by size: 5439 1496 443 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2661 2661 Size: 7471 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4596 4790 5532 5617 6688 1391 Size: 399 2806 194 742 85 1071 2174 Fragments arranged by size: 2806 2174 1071 742 399 194 85 BspEI T'CCGG_A Cuts at: 7134 7134 Size: 7471 BspHI T'CATG_A Cuts at: 5360 7090 5360 Size: 1730 5741 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4571 4826 844 Size: 3727 255 3489 Fragments arranged by size: 3727 3489 255 BsrBI CCG'CTC Cuts at: 1394 1823 5358 1394 Size: 429 3535 3507 Fragments arranged by size: 3535 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3595 5966 6140 65 Size: 1183 2347 2371 174 1396 Fragments arranged by size: 2371 2347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6115 6302 1700 Size: 110 4305 187 2869 Fragments arranged by size: 4305 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7471 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7471 BssSI C'ACGA_G Cuts at: 3525 5531 6543 3525 Size: 2006 1012 4453 Fragments arranged by size: 4453 2006 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7471 BstBI TT'CG_AA Cuts at: 6275 6275 Size: 7471 BstEII G'GTnAC_C Cuts at: 1184 4653 1184 Size: 3469 4002 BstXI CCAn_nnnn'nTGG Cuts at: 3066 3387 3507 4243 3066 Size: 321 120 736 6294 Fragments arranged by size: 6294 736 321 120 BstYI r'GATC_y Cuts at: 1972 2842 3013 3082 3460 3580 4687 4801 5168 Size: 870 171 69 378 120 1107 114 367 Cuts at: 5168 5553 5570 7022 7108 7120 7466 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1107 870 385 378 367 346 171 120 114 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3243 3999 513 Size: 552 939 1239 756 3985 Fragments arranged by size: 3985 1239 939 756 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2243 487 Size: 683 56 1017 5715 Fragments arranged by size: 5715 1017 683 56 BtsI GCAGTG_nn' Cuts at: 2010 4987 5781 5801 2010 Size: 2977 794 20 3680 Fragments arranged by size: 3680 2977 794 20 ClaI AT'CG_AT Cuts at: 1554 4026 1554 Size: 2472 4999 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7436 DraI TTT'AAA Cuts at: 2268 4775 2268 Size: 2507 4964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6478 809 Size: 507 5162 1802 Fragments arranged by size: 5162 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4918 5807 Size: 54 1425 50 275 18 177 2911 889 Cuts at: 5807 7436 8 Size: 1629 43 Fragments arranged by size: 2911 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4918 1537 Size: 275 3106 4090 Fragments arranged by size: 4090 3106 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3166 3184 4365 4740 5401 1353 Size: 1813 18 1181 375 661 3423 Fragments arranged by size: 3423 1813 1181 661 375 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6058 6432 6578 387 Size: 1064 138 4469 374 146 1280 Fragments arranged by size: 4469 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4594 4594 Size: 7471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2769 2817 2832 2940 3141 4241 Size: 393 876 261 48 15 108 201 1100 Cuts at: 4241 4346 5534 6131 6918 7186 1239 Size: 105 1188 597 787 268 1524 Fragments arranged by size: 1524 1188 1100 876 787 597 393 268 261 201 108 105 48 15 EcoNI CCTnn'n_nnAGG Cuts at: 4578 4578 Size: 7471 EcoRI G'AATT_C Cuts at: 4780 5158 4780 Size: 378 7093 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3836 4349 3836 Size: 513 6958 FspI TGC'GCA Cuts at: 5977 5977 Size: 7471 HaeII r_GCGC'y Cuts at: 1678 3790 6618 1678 Size: 2112 2828 2531 Fragments arranged by size: 2828 2531 2112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 4079 4111 6112 Size: 32 135 32 1181 32 1294 32 2001 Cuts at: 6112 6144 6186 6218 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2001 1294 1181 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4183 4426 4815 5061 669 Size: 988 281 2245 243 389 246 3079 Fragments arranged by size: 3079 2245 988 389 281 246 243 HindIII A'AGCT_T Cuts at: 748 3653 4326 748 Size: 2905 673 3893 Fragments arranged by size: 3893 2905 673 HpaI GTT'AAC Cuts at: 5061 5061 Size: 7471 KpnI G_GTAC'C Cuts at: 4796 4796 Size: 7471 MfeI C'AATT_G Cuts at: 3290 5070 3290 Size: 1780 5691 MluI A'CGCG_T Cuts at: 7347 7347 Size: 7471 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2495 3772 6585 6769 1305 Size: 1190 1277 2813 184 2007 Fragments arranged by size: 2813 2007 1277 1190 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5472 Fragments arranged by size: 5472 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2511 3064 5429 5788 5947 518 Size: 648 1345 553 2365 359 159 2042 Fragments arranged by size: 2365 2042 1345 648 553 359 159 MspA1I CmG'CkG Cuts at: 1606 3235 3283 4887 5564 6712 6957 1606 Size: 1629 48 1604 677 1148 245 2120 Fragments arranged by size: 2120 1629 1604 1148 677 245 48 NcoI C'CATG_G Cuts at: 513 1065 2004 3243 3999 513 Size: 552 939 1239 756 3985 Fragments arranged by size: 3985 1239 939 756 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7471 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7471 NotI GC'GGCC_GC Cuts at: 4918 4918 Size: 7471 NspI r_CATG'y Cuts at: 1132 1229 4417 4674 4829 6374 1132 Size: 97 3188 257 155 1545 2229 Fragments arranged by size: 3188 2229 1545 257 155 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7471 PciI A'CATG_T Cuts at: 4670 6370 4670 Size: 1700 5771 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7471 PmeI GTTT'AAAC Cuts at: 4775 4775 Size: 7471 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5540 5572 7053 7085 5540 Size: 32 1481 32 5926 Fragments arranged by size: 5926 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7471 PsiI TTA'TAA Cuts at: 4116 5041 4116 Size: 925 6546 PstI C_TGCA'G Cuts at: 830 2462 3355 3930 4823 830 Size: 1632 893 575 893 3478 Fragments arranged by size: 3478 1632 893 893 575 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7471 SacI G_AGCT'C Cuts at: 4596 4596 Size: 7471 SalI G'TCGA_C Cuts at: 1936 4813 1936 Size: 2877 4594 SbfI CC_TGCA'GG Cuts at: 4823 4823 Size: 7471 ScaI AGT'ACT Cuts at: 2830 4376 5719 7431 2830 Size: 1546 1343 1712 2870 Fragments arranged by size: 2870 1712 1546 1343 SfcI C'TryA_G Cuts at: 826 1045 2458 3351 3926 4370 4819 5954 6635 Size: 219 1413 893 575 444 449 1135 681 Cuts at: 6635 6826 826 Size: 191 1471 Fragments arranged by size: 1471 1413 1135 893 681 575 449 444 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7471 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7471 SmaI CCC'GGG Cuts at: 1872 4798 1872 Size: 2926 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3471 3778 4302 5575 6476 6738 Size: 197 926 1528 307 524 1273 901 262 Cuts at: 6738 820 Size: 1553 Fragments arranged by size: 1553 1528 1273 926 901 524 307 262 197 SnaBI TAC'GTA Cuts at: 493 5262 493 Size: 4769 2702 SpeI A'CTAG_T Cuts at: 152 2033 2825 152 Size: 1881 792 4798 Fragments arranged by size: 4798 1881 792 SphI G_CATG'C Cuts at: 1132 4417 4829 1132 Size: 3285 412 3774 Fragments arranged by size: 3774 3285 412 SspI AAT'ATT Cuts at: 5 52 3801 4240 5395 5 Size: 47 3749 439 1155 2081 Fragments arranged by size: 3749 2081 1155 439 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7471 StyI C'CwwG_G Cuts at: 513 1065 2004 2021 2370 3243 3999 4910 513 Size: 552 939 17 349 873 756 911 3074 Fragments arranged by size: 3074 939 911 873 756 552 349 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2994 5498 5515 5668 5853 6298 325 Size: 1255 1414 2504 17 153 185 445 1498 Fragments arranged by size: 2504 1498 1414 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2828 4170 4309 Size: 274 80 33 51 1443 851 1342 139 Cuts at: 4309 4374 5717 7429 96 Size: 65 1343 1712 138 Fragments arranged by size: 1712 1443 1343 1342 851 274 139 138 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2767 2799 7431 7463 2767 Size: 32 4632 32 2775 Fragments arranged by size: 4632 2775 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7471 XbaI T'CTAG_A Cuts at: 2957 4807 2957 Size: 1850 5621 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2908 2908 Size: 7471 XhoI C'TCGA_G Cuts at: 1943 4302 1943 Size: 2359 5112 XmaI C'CCGG_G Cuts at: 1870 4796 1870 Size: 2926 4545 XmnI GAAnn'nnTTC Cuts at: 2108 2889 2965 4353 4728 5600 2108 Size: 781 76 1388 375 872 3979 Fragments arranged by size: 3979 1388 872 781 375 76 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5279 276 Size: 53 83 186 936 3745 2468 Fragments arranged by size: 3745 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI