(Circular) (Six-base) MAPSORT of: pFN21AB7171.seq Check: 8875 from: 1 to: 6151 pFN21AB7171 6151 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 3361 4214 5598 5866 1632 Size: 542 1187 853 1384 268 1917 Fragments arranged by size: 1917 1384 1187 853 542 268 AfeI AGC'GCT Cuts at: 2902 2902 Size: 6151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 2319 3392 5050 6027 1203 Size: 1116 1073 1658 977 1327 Fragments arranged by size: 1658 1327 1116 1073 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 3894 5466 3894 Size: 1572 4579 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2528 3360 3460 3686 3838 5947 1493 Size: 1035 832 100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 832 226 152 100 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 AvrII C'CTAG_G Cuts at: 2732 2732 Size: 6151 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2067 3390 3470 1391 Size: 676 1323 80 4072 Fragments arranged by size: 4072 1323 676 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2326 2680 3076 928 Size: 971 427 354 396 4003 Fragments arranged by size: 4003 971 427 396 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 2662 1548 Size: 1114 5037 BfrBI ATG'CAT Cuts at: 3264 3264 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 4212 5368 1790 Size: 2422 1156 2573 Fragments arranged by size: 2573 2422 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 2120 2808 Size: 416 53 83 399 24 646 57 688 Cuts at: 2808 3882 4840 442 Size: 1074 958 1753 Fragments arranged by size: 1753 1074 958 688 646 416 399 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2524 2637 3386 4811 1239 Size: 1001 284 113 749 1425 2579 Fragments arranged by size: 2579 1425 1001 749 284 113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4276 5141 5439 4276 Size: 865 298 4988 Fragments arranged by size: 4988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 2540 2595 2803 4814 882 Size: 617 519 522 55 208 2011 2219 Fragments arranged by size: 2219 2011 617 522 519 208 55 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 2982 6145 2982 Size: 3163 2988 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3958 4340 4975 Size: 53 83 186 936 476 1949 382 635 Cuts at: 4975 275 Size: 1451 Fragments arranged by size: 1949 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5926 Fragments arranged by size: 5926 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 3398 1186 Size: 1015 1197 3939 Fragments arranged by size: 3939 1197 1015 BseYI C'CCAG_C Cuts at: 5354 5850 5354 Size: 496 5655 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5578 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3390 4212 4297 5368 3390 Size: 822 85 1071 4173 Fragments arranged by size: 4173 1071 822 85 BsmI GAATG_Cn' Cuts at: 2466 3096 3662 3755 2466 Size: 630 566 93 4862 Fragments arranged by size: 4862 630 566 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 3390 3470 4212 4297 5368 1391 Size: 399 277 1323 80 742 85 1071 2174 Fragments arranged by size: 2174 1323 1071 742 399 277 85 80 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 3057 4040 5770 3057 Size: 983 1730 3438 Fragments arranged by size: 3438 1730 983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 2794 3506 844 Size: 1424 526 712 3489 Fragments arranged by size: 3489 1424 712 526 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2131 2413 3203 3264 4646 4820 65 Size: 1183 883 282 790 61 1382 174 1396 Fragments arranged by size: 1396 1382 1183 883 790 282 174 61 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 3002 4211 5223 3002 Size: 1209 1012 3930 Fragments arranged by size: 3930 1209 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2851 3191 2356 Size: 495 340 5316 Fragments arranged by size: 5316 495 340 BstYI r'GATC_y Cuts at: 1972 2572 2638 3481 3848 4233 4250 5702 5788 Size: 600 66 843 367 385 17 1452 86 Cuts at: 5788 5800 6146 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 843 600 385 367 346 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 2082 3141 513 Size: 552 939 50 28 1059 3523 Fragments arranged by size: 3523 1059 939 552 50 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2387 487 Size: 683 56 1161 4251 Fragments arranged by size: 4251 1161 683 56 BtsI GCAGTG_nn' Cuts at: 3667 4461 4481 3667 Size: 794 20 5337 Fragments arranged by size: 5337 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 2782 2886 3455 2782 Size: 104 569 5478 Fragments arranged by size: 5478 569 104 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3138 3598 4487 Size: 54 1425 50 275 18 1308 460 889 Cuts at: 4487 6116 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1308 889 460 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4081 1353 Size: 2728 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2219 4738 5112 5258 387 Size: 1064 138 630 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 630 374 146 138 EcoICRI GAG'CTC Cuts at: 3388 3388 Size: 6151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2240 2524 2637 3361 3386 4214 Size: 393 542 66 284 113 724 25 828 Cuts at: 4214 4811 5598 5866 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 828 787 724 597 542 393 284 268 113 66 25 EcoNI CCTnn'n_nnAGG Cuts at: 2275 2275 Size: 6151 EcoRI G'AATT_C Cuts at: 3360 3460 3838 3360 Size: 100 378 5673 Fragments arranged by size: 5673 378 100 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 2904 5298 1678 Size: 1226 2394 2531 Fragments arranged by size: 2531 2394 1226 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2630 2662 2783 Size: 32 135 32 458 32 568 32 121 Cuts at: 2783 2815 4792 4824 4866 4898 1373 Size: 32 1977 32 42 32 2626 Fragments arranged by size: 2626 1977 568 458 135 121 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 3495 3741 669 Size: 988 281 813 744 246 3079 Fragments arranged by size: 3079 988 813 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 2751 3741 2751 Size: 990 5161 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2716 5265 5449 1305 Size: 1411 2549 184 2007 Fragments arranged by size: 2549 2007 1411 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2053 2406 2471 4109 4468 4627 518 Size: 648 887 353 65 1638 359 159 2042 Fragments arranged by size: 2042 1638 887 648 359 353 159 65 MspA1I CmG'CkG Cuts at: 1606 2164 3567 4244 5392 5637 1606 Size: 558 1403 677 1148 245 2120 Fragments arranged by size: 2120 1403 1148 677 558 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2054 2082 513 Size: 552 939 50 28 4582 Fragments arranged by size: 4582 939 552 50 28 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NsiI A_TGCA'T Cuts at: 3266 3266 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2323 3509 5054 1132 Size: 97 1094 1186 1545 2229 Fragments arranged by size: 2229 1545 1186 1094 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 2319 5050 2319 Size: 2731 3420 PflMI CCAn_nnn'nTGG Cuts at: 1108 2126 1108 Size: 1018 5133 PfoI T'CCnGG_A Cuts at: 2946 2946 Size: 6151 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PstI C_TGCA'G Cuts at: 830 2722 3503 830 Size: 1892 781 3478 Fragments arranged by size: 3478 1892 781 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 SacI G_AGCT'C Cuts at: 3390 3390 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 2499 2718 3499 4634 5315 5506 826 Size: 219 1454 219 781 1135 681 191 1471 Fragments arranged by size: 1471 1454 1135 781 681 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4255 5156 5418 820 Size: 197 926 2312 901 262 1553 Fragments arranged by size: 2312 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 2054 2082 2199 2669 2732 3228 Size: 552 939 50 28 117 470 63 496 Cuts at: 3228 3590 513 Size: 362 3074 Fragments arranged by size: 3074 939 552 496 470 362 117 63 50 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 2434 3416 4178 4195 4348 4533 Size: 1255 551 303 982 762 17 153 185 Cuts at: 4533 4978 325 Size: 445 1498 Fragments arranged by size: 1498 1255 982 762 551 445 303 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3440 4397 6109 Size: 274 80 33 51 1443 1463 957 1712 Cuts at: 6109 96 Size: 138 Fragments arranged by size: 1712 1463 1443 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2793 2825 2928 2960 3391 3423 6111 6143 2793 Size: 32 103 32 431 32 2688 32 2801 Fragments arranged by size: 2801 2688 431 103 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2516 2516 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI