Restriction map for pFN21AB7171
 (Linear) (Six-base) MAPSORT of: KIBB7171.seq  Check: 2168  from: 1  to: 1446



KIBB7171.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169    1356    1446 
   Size:        169    1187      90

  Fragments arranged by size: 

                1187     169      90
 
AfeI AGC'GCT

Cuts at:      0     897    1446 
   Size:        897     549
 
AflIII A'CryG_T

Cuts at:      0     314    1387    1446 
   Size:        314    1073      59

  Fragments arranged by size: 

                1073     314      59
 
ApoI r'AATT_y

Cuts at:      0     523    1355    1446 
   Size:        523     832      91

  Fragments arranged by size: 

                 832     523      91
 
AvrII C'CTAG_G

Cuts at:      0     727    1446 
   Size:        727     719
 
BanII G_rGCy'C

Cuts at:      0      62    1385    1446 
   Size:         62    1323      61

  Fragments arranged by size: 

                1323      62      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     321     675    1071    1446 
   Size:        321     354     396     375

  Fragments arranged by size: 

                 396     375     354     321
 
BclI T'GATC_A

Cuts at:      0     657    1446 
   Size:        657     789
 
BfrBI ATG'CAT

Cuts at:      0    1259    1446 
   Size:       1259     187
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      58     115     803    1446 
   Size:         58      57     688     643

  Fragments arranged by size: 

                 688     643      58      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     519     632    1381    1446 
   Size:        235     284     113     749      65

  Fragments arranged by size: 

                 749     284     235     113      65
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      13     535     590     798    1446 
   Size:         13     522      55     208     648

  Fragments arranged by size: 

                 648     522     208      55      13
 
BsaBI GATnn'nnATC

Cuts at:      0     977    1446 
   Size:        977     469
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1446 
   Size:          4    1442
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     196    1393    1446 
   Size:        196    1197      53

  Fragments arranged by size: 

                1197     196      53
 
BsiHKAI G_wGCw'C

Cuts at:      0    1385    1446 
   Size:       1385      61
 
BsmI GAATG_Cn'

Cuts at:      0     461    1091    1446 
   Size:        461     630     355

  Fragments arranged by size: 

                 630     461     355
 
Bsp1286I G_dGCh'C

Cuts at:      0      62    1385    1446 
   Size:         62    1323      61

  Fragments arranged by size: 

                1323      62      61
 
BspHI T'CATG_A

Cuts at:      0    1052    1446 
   Size:       1052     394
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263     789    1446 
   Size:        263     526     657

  Fragments arranged by size: 

                 657     526     263
 
BsrDI GCAATG_nn'

Cuts at:      0     126     408    1198    1259    1446 
   Size:        126     282     790      61     187

  Fragments arranged by size: 

                 790     282     187     126      61
 
BssSI C'ACGA_G

Cuts at:      0     997    1446 
   Size:        997     449
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     351     846    1186    1446 
   Size:        351     495     340     260

  Fragments arranged by size: 

                 495     351     340     260
 
BstYI r'GATC_y

Cuts at:      0     567     633    1446 
   Size:        567      66     813

  Fragments arranged by size: 

                 813     567      66
 
BtgI C'CryG_G

Cuts at:      0      49      77    1136    1446 
   Size:         49      28    1059     310

  Fragments arranged by size: 

                1059     310      49      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     382    1446 
   Size:        382    1064
 
DraI TTT'AAA

Cuts at:      0     777     881    1446 
   Size:        777     104     565

  Fragments arranged by size: 

                 777     565     104
 
EaeI y'GGCC_r

Cuts at:      0    1133    1446 
   Size:       1133     313
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     214    1446 
   Size:        214    1232
 
EcoICRI GAG'CTC

Cuts at:      0    1383    1446 
   Size:       1383      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     235     519     632    1356    1381    1446 
   Size:        169      66     284     113     724      25      65

  Fragments arranged by size: 

                 724     284     169     113      66      65      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     270    1446 
   Size:        270    1176
 
EcoRI G'AATT_C

Cuts at:      0    1355    1446 
   Size:       1355      91
 
HaeII r_GCGC'y

Cuts at:      0     899    1446 
   Size:        899     547
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     625     657     778     810    1446 
   Size:         25      32     568      32     121      32     636

  Fragments arranged by size: 

                 636     568     121      32      32      32      25
 
HincII GTy'rAC

Cuts at:      0     746    1446 
   Size:        746     700
 
HpaI GTT'AAC

Cuts at:      0     746    1446 
   Size:        746     700
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     711    1446 
   Size:        711     735
 
MslI CAynn'nnrTG

Cuts at:      0      48     401     466    1446 
   Size:         48     353      65     980

  Fragments arranged by size: 

                 980     353      65      48
 
MspA1I CmG'CkG

Cuts at:      0     159    1446 
   Size:        159    1287
 
NcoI C'CATG_G

Cuts at:      0      49      77    1446 
   Size:         49      28    1369

  Fragments arranged by size: 

                1369      49      28
 
NsiI A_TGCA'T

Cuts at:      0    1261    1446 
   Size:       1261     185
 
NspI r_CATG'y

Cuts at:      0     318    1446 
   Size:        318    1128
 
PciI A'CATG_T

Cuts at:      0     314    1446 
   Size:        314    1132
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     121    1446 
   Size:        121    1325
 
PfoI T'CCnGG_A

Cuts at:      0     941    1446 
   Size:        941     505
 
PstI C_TGCA'G

Cuts at:      0     717    1446 
   Size:        717     729
 
SacI G_AGCT'C

Cuts at:      0    1385    1446 
   Size:       1385      61
 
SfcI C'TryA_G

Cuts at:      0     494     713    1446 
   Size:        494     219     733

  Fragments arranged by size: 

                 733     494     219
 
StyI C'CwwG_G

Cuts at:      0      49      77     194     664     727    1223    1446 
   Size:         49      28     117     470      63     496     223

  Fragments arranged by size: 

                 496     470     223     117      63      49      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     126     429    1411    1446 
   Size:        126     303     982      35

  Fragments arranged by size: 

                 982     303     126      35
 
TatI w'GTAC_w

Cuts at:      0    1435    1446 
   Size:       1435      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     788     820     923     955    1386    1418    1446 
   Size:        788      32     103      32     431      32      28

  Fragments arranged by size: 

                 788     431     103      32      32      32      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     511    1446 
   Size:        511     935
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     ApoI    AvrII    BanII     BbsI     BclI
    BfrBI     BmrI     BpmI     BsaI    BsaBI    BsaHI    BseRI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BssSI    BstXI    BstYI
     BtgI    BtgZI     DraI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    HaeII    Hin4I   HincII     HpaI     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI     PciI    PflMI     PfoI     PstI     SacI
     SfcI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EarI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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