Restriction map for
pFN21AB6877
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 200 GGTACC G^GTACC
AccI 67 GTCGAC GT^[AC][GT]AC
AciI 112 CCGC CCGC(-3/-1)
AfaI 201 GTAC GT^AC
AgeI 509 ACCGGT A^CCGGT
AlwNI 309 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 200 GGTACC G^GTACC
AvaI 18 CCCGGG C^[CT]CG[GA]G
BalI 437 TGGCCA TGG^CCA
BamHI 408 GGATCC G^GATCC
BanI 200 GGTACC G^G[CT][GA]CC
BbsI 473 GTCTTC GAAGAC(2/6)
BbvI 427 GCTGC GCAGC(8/12)
BpuAI 473 GTCTTC GAAGAC(2/6)
BsaWI 509 ACCGGT [AT]^CCGG[AT]
BsgI 346 CTGCAC GTGCAG(16/14)
BsoBI 18 CCCGGG C^[CT]CG[GA]G
BsrDI 478 CATTGC GCAATG(2/0)
BsrFI 509 ACCGGT [GA]^CCGG[CT]
BstEII 361 GGTGACC G^GT[ACGT]ACC
BstF5I 168 CATCC GGATG(2/0)
BstPI 361 GGTGACC G^GT[ACGT]ACC
BstXI 233 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 408 GGATCC [GA]^GATC[CT]
CfoI 297 GCGC GCG^C
Cfr10I 509 ACCGGT [GA]^CCGG[CT]
EaeI 437 TGGCCA [CT]^GGCC[GA]
EcoNI 351 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 361 GGTGACC G^GT[ACGT]ACC
FokI 168 CATCC GGATG(9/13)
HhaI 297 GCGC GCG^C
HinP1I 297 GCGC G^CGC
HincII 67 GTCGAC GT[CT]^[GA]AC
HindII 67 GTCGAC GT[CT]^[GA]AC
HinfI 245 GACTC G^A[ACGT]TC
KpnI 200 GGTACC GGTAC^C
MflI 408 GGATCC [GA]^GATC[CT]
MluNI 437 TGGCCA TGG^CCA
MscI 437 TGGCCA TGG^CCA
PinAI 509 ACCGGT A^CCGGT
PleI 245 GACTC GAGTC(4/5)
PstI 428 CTGCAG CTGCA^G
RsaI 201 GTAC GT^AC
SalI 67 GTCGAC G^TCGAC
SexAI 371 ACCAGGT A^CC[AT]GGT
SmaI 18 CCCGGG CCC^GGG
TseI 427 GCTGC G^C[AT]GC
XhoII 408 GGATCC [GA]^GATC[CT]
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 409 GATCC GGATC(4/5)
408 GGATC
BpmI 99 CTCCAG CTGGAG(16/14)
379 CTCCAG
BssSI 282 CACGAG CACGAG(-5/-1)
403 CTCGTG
Cac8I 120 GCAGGC GC[ACGT]^[ACGT]GC
423 GCCTGC
DpnII 343 GATC ^GATC
409 GATC
Fnu4HI 111 GCCGC GC^[ACGT]GC
427 GCTGC
HphI 361 GGTGA GGTGA(8/7)
305 TCACC
ItaI 111 GCCGC GC^[ACGT]GC
427 GCTGC
MaeIII 106 GTCAC ^GT[ACGT]AC
362 GTGAC
MboI 343 GATC ^GATC
409 GATC
MboII 257 TCTTC GAAGA(8/7)
474 TCTTC
NdeII 343 GATC ^GATC
409 GATC
Sau3AI 343 GATC ^GATC
409 GATC
SfaNI 133 GATGC GCATC(5/9)
525 GATGC
SfcI 81 CTACAG C^T[GA][CT]AG
428 CTGCAG
TaqI 68 TCGA T^CGA
131 TCGA
Tsp45I 106 GTCAC ^GT[GC]AC
362 GTGAC
TthHB8I 68 TCGA T^CGA
131 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AvaII BcnI BsaJI BsiYI
BslI BsmFI BspMI BssKI BstNI
Cfr13I DraII EcoO109I EcoRII HaeIII
HapII HpaII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
Sau96I ScrFI