Restriction map for
pFN21AB8907
(Linear) (Six-base) MAPSORT of: KIBB8907.seq Check: 30 from: 1 to: 717
KIBB8907.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 205 717
Size: 92 113 512
Fragments arranged by size:
512 113 92
AlwNI CAG_nnn'CTG
Cuts at: 0 237 717
Size: 237 480
ApaI G_GGCC'C
Cuts at: 0 481 717
Size: 481 236
BanI G'GyrC_C
Cuts at: 0 121 717
Size: 121 596
BanII G_rGCy'C
Cuts at: 0 355 481 492 717
Size: 355 126 11 225
Fragments arranged by size:
355 225 126 11
BbeI G_GCGC'C
Cuts at: 0 125 717
Size: 125 592
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 717
Size: 70 647
BglI GCCn_nnn'nGGC
Cuts at: 0 344 717
Size: 344 373
Bme1580I G_kGCm'C
Cuts at: 0 481 717
Size: 481 236
BmrI ACTGGGnnnn_n'
Cuts at: 0 519 717
Size: 519 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 603 717
Size: 348 255 114
Fragments arranged by size:
348 255 114
BsaHI Gr'CG_yC
Cuts at: 0 25 122 142 717
Size: 25 97 20 575
Fragments arranged by size:
575 97 25 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 717
Size: 298 419
BseYI C'CCAG_C
Cuts at: 0 108 377 457 660 717
Size: 108 269 80 203 57
Fragments arranged by size:
269 203 108 80 57
BsiEI CG_ry'CG
Cuts at: 0 411 717
Size: 411 306
Bsp1286I G_dGCh'C
Cuts at: 0 355 481 492 717
Size: 355 126 11 225
Fragments arranged by size:
355 225 126 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 212 717
Size: 41 171 505
Fragments arranged by size:
505 171 41
BsrBI CCG'CTC
Cuts at: 0 288 362 717
Size: 288 74 355
Fragments arranged by size:
355 288 74
BsrDI GCAATG_nn'
Cuts at: 0 306 717
Size: 306 411
BsrFI r'CCGG_y
Cuts at: 0 151 410 717
Size: 151 259 307
Fragments arranged by size:
307 259 151
BstEII G'GTnAC_C
Cuts at: 0 240 717
Size: 240 477
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 717
Size: 465 252
BstYI r'GATC_y
Cuts at: 0 224 717
Size: 224 493
BtgI C'CryG_G
Cuts at: 0 11 84 717
Size: 11 73 633
Fragments arranged by size:
633 73 11
BtsI GCAGTG_nn'
Cuts at: 0 272 697 717
Size: 272 425 20
Fragments arranged by size:
425 272 20
ClaI AT'CG_AT
Cuts at: 0 215 717
Size: 215 502
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 717
Size: 15 702
EaeI y'GGCC_r
Cuts at: 0 153 717
Size: 153 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 205 717
Size: 92 113 512
Fragments arranged by size:
512 113 92
EcoO109I rG'GnC_Cy
Cuts at: 0 477 717
Size: 477 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 681 717
Size: 681 36
HaeII r_GCGC'y
Cuts at: 0 125 184 717
Size: 125 59 533
Fragments arranged by size:
533 125 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 717
Size: 409 32 276
Fragments arranged by size:
409 276 32
HincII GTy'rAC
Cuts at: 0 299 717
Size: 299 418
KasI G'GCGC_C
Cuts at: 0 121 717
Size: 121 596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 717
Size: 260 457
MslI CAynn'nnrTG
Cuts at: 0 217 717
Size: 217 500
MspA1I CmG'CkG
Cuts at: 0 86 237 377 717
Size: 86 151 140 340
Fragments arranged by size:
340 151 140 86
NarI GG'CG_CC
Cuts at: 0 122 717
Size: 122 595
PasI CC'CwG_GG
Cuts at: 0 136 604 717
Size: 136 468 113
Fragments arranged by size:
468 136 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 717
Size: 162 555
PfoI T'CCnGG_A
Cuts at: 0 440 717
Size: 440 277
PspOMI G'GGCC_C
Cuts at: 0 477 717
Size: 477 240
PstI C_TGCA'G
Cuts at: 0 233 717
Size: 233 484
PvuII CAG'CTG
Cuts at: 0 237 377 717
Size: 237 140 340
Fragments arranged by size:
340 237 140
SacII CC_GC'GG
Cuts at: 0 87 717
Size: 87 630
SfcI C'TryA_G
Cuts at: 0 229 717
Size: 229 488
SfoI GGC'GCC
Cuts at: 0 123 717
Size: 123 594
SmlI C'TyrA_G
Cuts at: 0 363 582 717
Size: 363 219 135
Fragments arranged by size:
363 219 135
Tth111I GACn'n_nGTC
Cuts at: 0 421 717
Size: 421 296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 549 717
Size: 411 138 168
Fragments arranged by size:
411 168 138
Enzymes that do cut:
AcuI AlwNI ApaI BanI BanII BbeI BbsI BglI
Bme1580I BmrI BpuEI BsaHI BseRI BseYI BsiEI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI
BtsI ClaI DrdI EaeI Eco57MI EcoO109I FalI HaeII
Hin4I HincII KasI MmeI MslI MspA1I NarI PasI
PflMI PfoI PspOMI PstI PvuII SacII SfcI SfoI
SmlI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI CspCI DraI
DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI XbaI XhoI XmaI XmnI
ZraI