(Circular) (Six-base) MAPSORT of: pFN21AE0476.seq Check: 2290 from: 1 to: 5440 pFN21AE0476 5440 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2489 2629 2489 Size: 140 5300 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2511 2783 1937 Size: 574 272 4594 Fragments arranged by size: 4594 574 272 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 3503 4887 5155 1632 Size: 431 1440 1384 268 1917 Fragments arranged by size: 1917 1440 1384 431 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 2517 2589 4339 5316 1203 Size: 1314 72 1750 977 1327 Fragments arranged by size: 1750 1327 1314 977 72 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 3183 4755 3183 Size: 1572 3868 ApaI G_GGCC'C Cuts at: 2019 2172 2019 Size: 153 5287 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2354 2749 2975 3127 5236 1493 Size: 861 395 226 152 2109 1697 Fragments arranged by size: 2109 1697 861 395 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2201 2614 2765 1870 Size: 73 258 413 151 4545 Fragments arranged by size: 4545 413 258 151 73 BamHI G'GATC_C Cuts at: 2106 2205 2770 3137 2106 Size: 99 565 367 4409 Fragments arranged by size: 4409 565 367 99 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2296 2761 4216 618 Size: 325 217 627 347 162 465 1455 1842 Fragments arranged by size: 1842 1455 627 465 347 325 217 162 BanII G_rGCy'C Cuts at: 1391 2019 2172 2702 2759 1391 Size: 628 153 530 57 4072 Fragments arranged by size: 4072 628 530 153 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2704 928 Size: 971 805 3664 Fragments arranged by size: 3664 971 805 BbvCI CC'TCA_GC Cuts at: 2630 2630 Size: 5440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 2415 1548 Size: 867 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2310 4051 136 Size: 107 122 71 631 605 638 1741 1525 Fragments arranged by size: 1741 1525 638 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 2019 2137 2172 2301 3501 4657 1790 Size: 229 118 35 129 1200 1156 2573 Fragments arranged by size: 2573 1200 1156 229 129 118 35 BmgBI CAC'GTC Cuts at: 1318 2011 2592 1318 Size: 693 581 4166 Fragments arranged by size: 4166 693 581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2340 4100 1239 Size: 1101 1760 2579 Fragments arranged by size: 2579 1760 1101 Bpu10I CC'TnA_GC Cuts at: 2630 2630 Size: 5440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3565 4430 4728 3565 Size: 865 298 4277 Fragments arranged by size: 4277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2562 3247 3629 4264 Size: 53 83 186 936 1029 685 382 635 Cuts at: 4264 275 Size: 1451 Fragments arranged by size: 1451 1029 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5215 Fragments arranged by size: 5215 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2663 1186 Size: 1162 315 3963 Fragments arranged by size: 3963 1162 315 BseYI C'CCAG_C Cuts at: 2018 2191 4643 5139 2018 Size: 173 2452 496 2319 Fragments arranged by size: 2452 2319 496 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2385 1163 Size: 573 649 4218 Fragments arranged by size: 4218 649 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2890 3651 4679 1540 Size: 275 186 889 761 1028 2301 Fragments arranged by size: 2301 1028 889 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2468 3501 3586 4657 2468 Size: 1033 85 1071 3251 Fragments arranged by size: 3251 1071 1033 85 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2137 2172 2301 2468 2702 2759 Size: 399 229 118 35 129 167 234 57 Cuts at: 2759 3501 3586 4657 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 234 229 167 129 118 85 57 35 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2489 2629 2795 844 Size: 1645 140 166 3489 Fragments arranged by size: 3489 1645 166 140 BsrBI CCG'CTC Cuts at: 1394 1823 3327 1394 Size: 429 1504 3507 Fragments arranged by size: 3507 1504 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3935 4109 65 Size: 1183 2687 174 1396 Fragments arranged by size: 2687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 2461 3500 4512 2461 Size: 1039 1012 3389 Fragments arranged by size: 3389 1039 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 2572 1184 Size: 1388 4052 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2106 2205 2770 3137 3522 3539 4991 5077 Size: 134 99 565 367 385 17 1452 86 Cuts at: 5077 5089 5435 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 565 385 367 346 134 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2075 2075 Size: 5440 BtgI C'CryG_G Cuts at: 513 1065 2004 2271 2307 513 Size: 552 939 267 36 3646 Fragments arranged by size: 3646 939 552 267 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3750 3770 2956 Size: 794 20 4626 Fragments arranged by size: 4626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2584 2887 Size: 54 1425 50 275 18 418 336 303 Cuts at: 2887 3776 5405 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 418 336 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 3370 1353 Size: 1382 635 3423 Fragments arranged by size: 3423 1382 635 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4027 4401 4547 387 Size: 1064 138 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2340 3503 4100 4887 5155 1239 Size: 393 431 277 1163 597 787 268 1524 Fragments arranged by size: 1524 1163 787 597 431 393 277 268 EcoO109I rG'GnC_Cy Cuts at: 2015 2168 2213 2276 2015 Size: 153 45 63 5179 Fragments arranged by size: 5179 153 63 45 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 4587 1678 Size: 2909 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373 Size: 32 135 32 2509 32 42 32 2626 Fragments arranged by size: 2626 2509 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2611 2784 3030 669 Size: 988 281 673 173 246 3079 Fragments arranged by size: 3079 988 673 281 246 173 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 2517 5316 2517 Size: 2799 2641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2586 10 Size: 54 1425 343 754 2864 Fragments arranged by size: 2864 1425 754 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3398 3757 3916 518 Size: 648 843 1389 359 159 2042 Fragments arranged by size: 2042 1389 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2559 2856 3533 4681 4926 1606 Size: 953 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 677 297 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2271 2307 513 Size: 552 939 267 36 3646 Fragments arranged by size: 3646 939 552 267 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2798 4343 1132 Size: 97 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 97 PasI CC'CwG_GG Cuts at: 1348 2111 2282 1348 Size: 763 171 4506 Fragments arranged by size: 4506 763 171 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2213 2276 2213 Size: 63 5377 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PspOMI G'GGCC_C Cuts at: 2015 2168 2015 Size: 153 5287 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2226 2258 2226 Size: 32 5408 PstI C_TGCA'G Cuts at: 830 2792 830 Size: 1962 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SanDI GG'GwC_CC Cuts at: 2276 2276 Size: 5440 SapI GCTCTTCn'nnn_ Cuts at: 2735 2735 Size: 5440 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2788 3923 4604 4795 826 Size: 219 1743 1135 681 191 1471 Fragments arranged by size: 1743 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2203 2767 1872 Size: 331 564 4545 Fragments arranged by size: 4545 564 331 SmlI C'TyrA_G Cuts at: 820 1017 1943 3544 4445 4707 820 Size: 197 926 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2004 2035 2172 2271 2307 2879 513 Size: 552 939 31 137 99 36 572 3074 Fragments arranged by size: 3074 939 572 552 137 99 36 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 3467 3484 3637 3822 4267 325 Size: 1255 839 1048 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1048 839 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5400 5432 5400 Size: 32 5408 Tth111I GACn'n_nGTC Cuts at: 1316 2708 1316 Size: 1392 4048 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2201 2765 1870 Size: 331 564 4545 Fragments arranged by size: 4545 564 331 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI