Restriction map for pFN21AE0476
 (Circular) (Six-base) MAPSORT of: pFN21AE0476.seq  Check: 2290  from: 1  to: 5440



pFN21AE0476  5440 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2489    2629    2489 
   Size:        140    5300
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3250     278 
   Size:         53      83     186     936    1714    2468

  Fragments arranged by size: 

                2468    1714     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2511    2783    1937 
   Size:        574     272    4594

  Fragments arranged by size: 

                4594     574     272
 
Acc65I G'GTAC_C

Cuts at:   2761    2761 
   Size:       5440
 
AclI AA'CG_TT

Cuts at:   1563    3567    3940    1563 
   Size:       2004     373    3063

  Fragments arranged by size: 

                3063    2004     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2063    3503    4887    5155    1632 
   Size:        431    1440    1384     268    1917

  Fragments arranged by size: 

                1917    1440    1384     431     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5243
 
AflIII A'CryG_T

Cuts at:   1203    2517    2589    4339    5316    1203 
   Size:       1314      72    1750     977    1327

  Fragments arranged by size: 

                1750    1327    1314     977      72
 
AgeI A'CCGG_T

Cuts at:   4271    4271 
   Size:       5440
 
AhdI GACnn_n'nnGTC

Cuts at:   4169    4169 
   Size:       5440
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5440
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5440
 
AlwNI CAG_nnn'CTG

Cuts at:   3183    4755    3183 
   Size:       1572    3868
 
ApaI G_GGCC'C

Cuts at:   2019    2172    2019 
   Size:        153    5287
 
ApaLI G'TGCA_C

Cuts at:   3497    4653    3497 
   Size:       1156    4284
 
ApoI r'AATT_y

Cuts at:   1493    2354    2749    2975    3127    5236    1493 
   Size:        861     395     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     861     395     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3994     160 
   Size:        872    2962    1606

  Fragments arranged by size: 

                2962    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5440
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2201    2614    2765    1870 
   Size:         73     258     413     151    4545

  Fragments arranged by size: 

                4545     413     258     151      73
 
BamHI G'GATC_C

Cuts at:   2106    2205    2770    3137    2106 
   Size:         99     565     367    4409

  Fragments arranged by size: 

                4409     565     367      99
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2134    2296    2761    4216     618 
   Size:        325     217     627     347     162     465    1455    1842

  Fragments arranged by size: 

                1842    1455     627     465     347     325     217     162
            
 
BanII G_rGCy'C

Cuts at:   1391    2019    2172    2702    2759    1391 
   Size:        628     153     530      57    4072

  Fragments arranged by size: 

                4072     628     530     153      57
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2704     928 
   Size:        971     805    3664

  Fragments arranged by size: 

                3664     971     805
 
BbvCI CC'TCA_GC

Cuts at:   2630    2630 
   Size:       5440
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3631    3665    1903 
   Size:         34    1694      34    3678

  Fragments arranged by size: 

                3678    1694      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3332    4542    3332 
   Size:       1210    4230
 
BclI T'GATC_A

Cuts at:   1548    2415    1548 
   Size:        867    4573
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2310    4051     136 
   Size:        107     122      71     631     605     638    1741    1525

  Fragments arranged by size: 

                1741    1525     638     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5435    5435 
   Size:       5440
 
BlpI GC'TnA_GC

Cuts at:   2857    2857 
   Size:       5440
 
Bme1580I G_kGCm'C

Cuts at:   1790    2019    2137    2172    2301    3501    4657    1790 
   Size:        229     118      35     129    1200    1156    2573

  Fragments arranged by size: 

                2573    1200    1156     229     129     118      35
 
BmgBI CAC'GTC

Cuts at:   1318    2011    2592    1318 
   Size:        693     581    4166

  Fragments arranged by size: 

                4166     693     581
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3171    4129     442 
   Size:        416      53      83     399      24    1754     958    1753

  Fragments arranged by size: 

                1754    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5440
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2340    4100    1239 
   Size:       1101    1760    2579

  Fragments arranged by size: 

                2579    1760    1101
 
Bpu10I CC'TnA_GC

Cuts at:   2630    2630 
   Size:       5440
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3565    4430    4728    3565 
   Size:        865     298    4277

  Fragments arranged by size: 

                4277     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4103     882 
   Size:        617    2604    2219

  Fragments arranged by size: 

                2604    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3231     493 
   Size:        711    2027    2702

  Fragments arranged by size: 

                2702    2027     711
 
BsaBI GATnn'nnATC

Cuts at:   5434    5434 
   Size:       5440
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2562    3247    3629    4264 
   Size:         53      83     186     936    1029     685     382     635

Cuts at:   4264     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1029     936     685     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3873    4271    4545    4692    5103    3873 
   Size:        398     274     147     411    4210

  Fragments arranged by size: 

                4210     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5215

  Fragments arranged by size: 

                5215     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2348    2663    1186 
   Size:       1162     315    3963

  Fragments arranged by size: 

                3963    1162     315
 
BseYI C'CCAG_C

Cuts at:   2018    2191    4643    5139    2018 
   Size:        173    2452     496    2319

  Fragments arranged by size: 

                2452    2319     496     173
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2385    1163 
   Size:        573     649    4218

  Fragments arranged by size: 

                4218     649     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2890    3651    4679    1540 
   Size:        275     186     889     761    1028    2301

  Fragments arranged by size: 

                2301    1028     889     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2468    3501    3586    4657    2468 
   Size:       1033      85    1071    3251

  Fragments arranged by size: 

                3251    1071    1033      85
 
BsmI GAATG_Cn'

Cuts at:   2951    3044    2951 
   Size:         93    5347
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2019    2137    2172    2301    2468    2702    2759 
   Size:        399     229     118      35     129     167     234      57

Cuts at:   2759    3501    3586    4657    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     234     229     167     129
                 118      85      57      35
 
BspEI T'CCGG_A

Cuts at:   5103    5103 
   Size:       5440
 
BspHI T'CATG_A

Cuts at:   3329    5059    3329 
   Size:       1730    3710
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2489    2629    2795     844 
   Size:       1645     140     166    3489

  Fragments arranged by size: 

                3489    1645     166     140
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3327    1394 
   Size:        429    1504    3507

  Fragments arranged by size: 

                3507    1504     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3935    4109      65 
   Size:       1183    2687     174    1396

  Fragments arranged by size: 

                2687    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4084    4271    1700 
   Size:        110    2274     187    2869

  Fragments arranged by size: 

                2869    2274     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5440
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5440
 
BssSI C'ACGA_G

Cuts at:   2461    3500    4512    2461 
   Size:       1039    1012    3389

  Fragments arranged by size: 

                3389    1039    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5440
 
BstBI TT'CG_AA

Cuts at:   4244    4244 
   Size:       5440
 
BstEII G'GTnAC_C

Cuts at:   1184    2572    1184 
   Size:       1388    4052
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2011    2011 
   Size:       5440
 
BstYI r'GATC_y

Cuts at:   1972    2106    2205    2770    3137    3522    3539    4991    5077 
   Size:        134      99     565     367     385      17    1452      86

Cuts at:   5077    5089    5435    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     565     385     367     346     134      99
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2075    2075 
   Size:       5440
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2271    2307     513 
   Size:        552     939     267      36    3646

  Fragments arranged by size: 

                3646     939     552     267      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4701

  Fragments arranged by size: 

                4701     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2956    3750    3770    2956 
   Size:        794      20    4626

  Fragments arranged by size: 

                4626     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5440
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5405
 
DraI TTT'AAA

Cuts at:   2744    2744 
   Size:       5440
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4447     809 
   Size:        507    3131    1802

  Fragments arranged by size: 

                3131    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2248    2584    2887 
   Size:         54    1425      50     275      18     418     336     303

Cuts at:   2887    3776    5405       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     418     336     303     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2887    1537 
   Size:        275    1075    4090

  Fragments arranged by size: 

                4090    1075     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2735    3370    1353 
   Size:       1382     635    3423

  Fragments arranged by size: 

                3423    1382     635
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4027    4401    4547     387 
   Size:       1064     138    2438     374     146    1280

  Fragments arranged by size: 

                2438    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2063    2340    3503    4100    4887    5155    1239 
   Size:        393     431     277    1163     597     787     268    1524

  Fragments arranged by size: 

                1524    1163     787     597     431     393     277     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2015    2168    2213    2276    2015 
   Size:        153      45      63    5179

  Fragments arranged by size: 

                5179     153      63      45
 
EcoRI G'AATT_C

Cuts at:   2749    3127    2749 
   Size:        378    5062
 
FspI TGC'GCA

Cuts at:   3946    3946 
   Size:       5440
 
HaeII r_GCGC'y

Cuts at:   1678    4587    1678 
   Size:       2909    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4081    4113    4155    4187    1373 
   Size:         32     135      32    2509      32      42      32    2626

  Fragments arranged by size: 

                2626    2509     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2611    2784    3030     669 
   Size:        988     281     673     173     246    3079

  Fragments arranged by size: 

                3079     988     673     281     246     173
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5440
 
HpaI GTT'AAC

Cuts at:   3030    3030 
   Size:       5440
 
KpnI G_GTAC'C

Cuts at:   2765    2765 
   Size:       5440
 
MfeI C'AATT_G

Cuts at:   3039    3039 
   Size:       5440
 
MluI A'CGCG_T

Cuts at:   2517    5316    2517 
   Size:       2799    2641
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4554    4738    1305 
   Size:       3249     184    2007

  Fragments arranged by size: 

                3249    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2586      10 
   Size:         54    1425     343     754    2864

  Fragments arranged by size: 

                2864    1425     754     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    3398    3757    3916     518 
   Size:        648     843    1389     359     159    2042

  Fragments arranged by size: 

                2042    1389     843     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2559    2856    3533    4681    4926    1606 
   Size:        953     297     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     953     677     297     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2271    2307     513 
   Size:        552     939     267      36    3646

  Fragments arranged by size: 

                3646     939     552     267      36
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5440
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5440
 
NotI GC'GGCC_GC

Cuts at:   2887    2887 
   Size:       5440
 
NspI r_CATG'y

Cuts at:   1132    1229    2798    4343    1132 
   Size:         97    1569    1545    2229

  Fragments arranged by size: 

                2229    1569    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2111    2282    1348 
   Size:        763     171    4506

  Fragments arranged by size: 

                4506     763     171
 
PciI A'CATG_T

Cuts at:   4339    4339 
   Size:       5440
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5440
 
PmeI GTTT'AAAC

Cuts at:   2744    2744 
   Size:       5440
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3509    3541    5022    5054    3509 
   Size:         32    1481      32    3895

  Fragments arranged by size: 

                3895    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2213    2276    2213 
   Size:         63    5377
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5440
 
PsiI TTA'TAA

Cuts at:   3010    3010 
   Size:       5440
 
PspOMI G'GGCC_C

Cuts at:   2015    2168    2015 
   Size:        153    5287
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2226    2258    2226 
   Size:         32    5408
 
PstI C_TGCA'G

Cuts at:    830    2792     830 
   Size:       1962    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5440
 
SalI G'TCGA_C

Cuts at:   1936    2782    1936 
   Size:        846    4594
 
SanDI GG'GwC_CC

Cuts at:   2276    2276 
   Size:       5440
 
SapI GCTCTTCn'nnn_

Cuts at:   2735    2735 
   Size:       5440
 
SbfI CC_TGCA'GG

Cuts at:   2792    2792 
   Size:       5440
 
ScaI AGT'ACT

Cuts at:   3688    5400    3688 
   Size:       1712    3728
 
SfcI C'TryA_G

Cuts at:    826    1045    2788    3923    4604    4795     826 
   Size:        219    1743    1135     681     191    1471

  Fragments arranged by size: 

                1743    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5440
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5440
 
SmaI CCC'GGG

Cuts at:   1872    2203    2767    1872 
   Size:        331     564    4545

  Fragments arranged by size: 

                4545     564     331
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3544    4445    4707     820 
   Size:        197     926    1601     901     262    1553

  Fragments arranged by size: 

                1601    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3231     493 
   Size:       2738    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5440
 
SphI G_CATG'C

Cuts at:   1132    2798    1132 
   Size:       1666    3774
 
SspI AAT'ATT

Cuts at:      5      52    3364       5 
   Size:         47    3312    2081

  Fragments arranged by size: 

                3312    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5440
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2035    2172    2271    2307    2879     513 
   Size:        552     939      31     137      99      36     572    3074

  Fragments arranged by size: 

                3074     939     572     552     137      99      36      31
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2419    3467    3484    3637    3822    4267     325 
   Size:       1255     839    1048      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1048     839     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3686    5398      96 
   Size:        274      80      33      51    1443    1709    1712     138

  Fragments arranged by size: 

                1712    1709    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5400    5432    5400 
   Size:         32    5408
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2708    1316 
   Size:       1392    4048
 
XbaI T'CTAG_A

Cuts at:   2776    2776 
   Size:       5440
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5440
 
XmaI C'CCGG_G

Cuts at:   1870    2201    2765    1870 
   Size:        331     564    4545

  Fragments arranged by size: 

                4545     564     331
 
XmnI GAAnn'nnTTC

Cuts at:   3569    3569 
   Size:       5440
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3248     276 
   Size:         53      83     186     936    1714    2468

  Fragments arranged by size: 

                2468    1714     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BfrBI     BplI
    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI    EcoNI    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI     PmlI    PspXI    PvuII    RsrII     SacI    SacII
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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