Restriction map for
pFN21AE0476
(Linear) (Six-base) MAPSORT of: KIEE0476.seq Check: 7732 from: 1 to: 735
KIEE0476.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 484 624 735
Size: 484 140 111
Fragments arranged by size:
484 140 111
AccI GT'mk_AC
Cuts at: 0 506 735
Size: 506 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 735
Size: 58 677
AflIII A'CryG_T
Cuts at: 0 512 584 735
Size: 512 72 151
Fragments arranged by size:
512 151 72
ApaI G_GGCC'C
Cuts at: 0 14 167 735
Size: 14 153 568
Fragments arranged by size:
568 153 14
ApoI r'AATT_y
Cuts at: 0 349 735
Size: 349 386
AvaI C'yCGr_G
Cuts at: 0 196 609 735
Size: 196 413 126
Fragments arranged by size:
413 196 126
BamHI G'GATC_C
Cuts at: 0 101 200 735
Size: 101 99 535
Fragments arranged by size:
535 101 99
BanI G'GyrC_C
Cuts at: 0 129 291 735
Size: 129 162 444
Fragments arranged by size:
444 162 129
BanII G_rGCy'C
Cuts at: 0 14 167 697 735
Size: 14 153 530 38
Fragments arranged by size:
530 153 38 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 699 735
Size: 699 36
BbvCI CC'TCA_GC
Cuts at: 0 625 735
Size: 625 110
BclI T'GATC_A
Cuts at: 0 410 735
Size: 410 325
BglI GCCn_nnn'nGGC
Cuts at: 0 305 735
Size: 305 430
Bme1580I G_kGCm'C
Cuts at: 0 14 132 167 296 735
Size: 14 118 35 129 439
Fragments arranged by size:
439 129 118 35 14
BmgBI CAC'GTC
Cuts at: 0 6 587 735
Size: 6 581 148
Fragments arranged by size:
581 148 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 735
Size: 335 400
Bpu10I CC'TnA_GC
Cuts at: 0 625 735
Size: 625 110
BsaHI Gr'CG_yC
Cuts at: 0 557 735
Size: 557 178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 658 735
Size: 343 315 77
Fragments arranged by size:
343 315 77
BseYI C'CCAG_C
Cuts at: 0 13 186 735
Size: 13 173 549
Fragments arranged by size:
549 173 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 735
Size: 380 355
BsiHKAI G_wGCw'C
Cuts at: 0 463 735
Size: 463 272
Bsp1286I G_dGCh'C
Cuts at: 0 14 132 167 296 463 697 735
Size: 14 118 35 129 167 234 38
Fragments arranged by size:
234 167 129 118 38 35 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 484 624 735
Size: 484 140 111
Fragments arranged by size:
484 140 111
BssSI C'ACGA_G
Cuts at: 0 456 735
Size: 456 279
BstEII G'GTnAC_C
Cuts at: 0 567 735
Size: 567 168
BstYI r'GATC_y
Cuts at: 0 101 200 735
Size: 101 99 535
Fragments arranged by size:
535 101 99
Bsu36I CC'TnA_GG
Cuts at: 0 70 735
Size: 70 665
BtgI C'CryG_G
Cuts at: 0 266 302 735
Size: 266 36 433
Fragments arranged by size:
433 266 36
EaeI y'GGCC_r
Cuts at: 0 243 579 735
Size: 243 336 156
Fragments arranged by size:
336 243 156
EarI CTCTTCn'nnn_
Cuts at: 0 730 735
Size: 730 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 335 735
Size: 58 277 400
Fragments arranged by size:
400 277 58
EcoO109I rG'GnC_Cy
Cuts at: 0 10 163 208 271 735
Size: 10 153 45 63 464
Fragments arranged by size:
464 153 63 45 10
HincII GTy'rAC
Cuts at: 0 606 735
Size: 606 129
MluI A'CGCG_T
Cuts at: 0 512 735
Size: 512 223
MscI TGG'CCA
Cuts at: 0 581 735
Size: 581 154
MspA1I CmG'CkG
Cuts at: 0 554 735
Size: 554 181
NcoI C'CATG_G
Cuts at: 0 266 302 735
Size: 266 36 433
Fragments arranged by size:
433 266 36
PasI CC'CwG_GG
Cuts at: 0 106 277 735
Size: 106 171 458
Fragments arranged by size:
458 171 106
PpuMI rG'GwC_Cy
Cuts at: 0 208 271 735
Size: 208 63 464
Fragments arranged by size:
464 208 63
PspOMI G'GGCC_C
Cuts at: 0 10 163 735
Size: 10 153 572
Fragments arranged by size:
572 153 10
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 221 253 735
Size: 221 32 482
Fragments arranged by size:
482 221 32
SanDI GG'GwC_CC
Cuts at: 0 271 735
Size: 271 464
SapI GCTCTTCn'nnn_
Cuts at: 0 730 735
Size: 730 5
SmaI CCC'GGG
Cuts at: 0 198 735
Size: 198 537
StyI C'CwwG_G
Cuts at: 0 30 167 266 302 735
Size: 30 137 99 36 433
Fragments arranged by size:
433 137 99 36 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 735
Size: 414 321
Tth111I GACn'n_nGTC
Cuts at: 0 703 735
Size: 703 32
XmaI C'CCGG_G
Cuts at: 0 196 735
Size: 196 539
Enzymes that do cut:
AarI AccI AcuI AflIII ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BclI BglI Bme1580I BmgBI
BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BssSI BstEII BstYI Bsu36I BtgI EaeI EarI
Eco57MI EcoO109I HincII MluI MscI MspA1I NcoI PasI
PpuMI PspOMI PsrI SanDI SapI SmaI StyI TaqII
Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BglII BlpI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MmeI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI XbaI XcmI XhoI
XmnI ZraI