Restriction map for
pFN21AB6384
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MslI MspA1I MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 475 GTCTAC GT^[AC][GT]AC
AccII 74 CGCG CG^CG
AlwI 739 GATCC GGATC(4/5)
AocI 827 CCTCAGG CC^T[ACGT]AGG
ApaI 823 GGGCCC GGGCC^C
AspHI 253 GAGCAC G[AT]GC[AT]^C
AvaI 21 CCCGGG C^[CT]CG[GA]G
BalI 813 TGGCCA TGG^CCA
BanII 823 GGGCCC G[GA]GC[CT]^C
BclI 390 TGATCA T^GATCA
BglI 606 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 515 AGATCT A^GATCT
BsaBI 771 GATCGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 712 GAGACC GGTCTC(1/5)
BsaWI 838 TCCGGT [AT]^CCGG[AT]
BsgI 799 GTGCAG GTGCAG(16/14)
BsiHKAI 253 GAGCAC G[AT]GC[AT]^C
BsmAI 712 GAGAC GTCTC(1/5)
BsoBI 21 CCCGGG C^[CT]CG[GA]G
BstEII 108 GGTGACC G^GT[ACGT]ACC
BstPI 108 GGTGACC G^GT[ACGT]ACC
BstUI 74 CGCG CG^CG
Bsu36I 827 CCTCAGG CC^T[ACGT]AGG
CfoI 75 GCGC GCG^C
DrdI 833 GACCCTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 813 TGGCCA [CT]^GGCC[GA]
EarI 709 GAAGAG CTCTTC(1/4)
Eco81I 827 CCTCAGG CC^T[ACGT]AGG
EcoO65I 108 GGTGACC G^GT[ACGT]ACC
FbaI 390 TGATCA T^GATCA
HhaI 75 GCGC GCG^C
HinP1I 75 GCGC G^CGC
Ksp632I 709 GAAGAG CTCTTC(1/4)
MaeII 138 ACGT A^CGT
MamI 771 GATCGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 813 TGGCCA TGG^CCA
MscI 813 TGGCCA TGG^CCA
MvnI 74 CGCG CG^CG
NdeI 142 CATATG CA^TATG
NspI 668 GCATGT [GA]CATG^[CT]
PflMI 653 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 831 AGGACCC [GA]G^G[AT]CC[CT]
SbfI 805 CCTGCAGG CCTGCA^GG
SmaI 21 CCCGGG CCC^GGG
Sse8387I 805 CCTGCAGG CCTGCA^GG
Tsp509I 282 AATT ^AATT
Van91I 653 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 43 CCGC CCGC(-3/-1)
579 CCGC
AvaII 832 GGACC G^G[AT]CC
841 GGTCC
BbsI 505 GAAGAC GAAGAC(2/6)
318 GTCTTC
BfaI 82 CTAG C^TAG
736 CTAG
BmyI 253 GAGCAC G[AGT]GC[ACT]^C
823 GGGCCC
BpmI 49 CTGGAG CTGGAG(16/14)
361 CTGGAG
BpuAI 505 GAAGAC GAAGAC(2/6)
318 GTCTTC
BsmFI 18 GTCCC GGGAC(10/14)
842 GTCCC
Bsp1286I 253 GAGCAC G[AGT]GC[ACT]^C
823 GGGCCC
BspMI 698 ACCTGC ACCTGC(4/8)
808 GCAGGT
BsrDI 449 GCAATG GCAATG(2/0)
657 GCAATG
BstF5I 78 CATCC GGATG(2/0)
249 GGATG
BstYI 515 AGATCT [GA]^GATC[CT]
738 AGATCC
FokI 78 CATCC GGATG(9/13)
249 GGATG
MaeI 82 CTAG C^TAG
736 CTAG
MaeIII 100 GTGAC ^GT[ACGT]AC
109 GTGAC
MflI 515 AGATCT [GA]^GATC[CT]
738 AGATCC
MseI 284 TTAA T^TAA
755 TTAA
PstI 33 CTGCAG CTGCA^G
806 CTGCAG
SfcI 33 CTGCAG C^T[GA][CT]AG
806 CTGCAG
TfiI 85 GAATC G^A[AT]TC
442 GATTC
Tru9I 284 TTAA T^TAA
755 TTAA
Tsp45I 100 GTGAC ^GT[GC]AC
109 GTGAC
XhoII 515 AGATCT [GA]^GATC[CT]
738 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI BbvI BcnI BsaJI BseRI
BsiYI BslI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI