(Circular) (Six-base) MAPSORT of: pFN21AB6384.seq Check: 5143 from: 1 to: 5605 pFN21AB6384 5605 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2804 2804 Size: 5605 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2481 2948 1937 Size: 544 467 4594 Fragments arranged by size: 4594 544 467 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 3668 5052 5320 1632 Size: 579 1457 1384 268 1917 Fragments arranged by size: 1917 1457 1384 579 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 4504 5481 1203 Size: 3301 977 1327 Fragments arranged by size: 3301 1327 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 3348 4920 3348 Size: 1572 4033 ApaI G_GGCC'C Cuts at: 2832 2832 Size: 5605 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2930 1870 Size: 73 83 904 4545 Fragments arranged by size: 4545 904 83 73 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 4381 618 Size: 325 217 627 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 BanII G_rGCy'C Cuts at: 1391 2832 2924 1391 Size: 1441 92 4072 Fragments arranged by size: 4072 1441 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2316 2517 928 Size: 971 417 201 4016 Fragments arranged by size: 4016 971 417 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 2395 1548 Size: 847 4758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2617 4216 136 Size: 107 122 71 631 605 945 1599 1525 Fragments arranged by size: 1599 1525 945 631 605 122 107 71 BglII A'GATC_T Cuts at: 2520 5600 2520 Size: 3080 2525 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2832 3666 4822 1790 Size: 1042 834 1156 2573 Fragments arranged by size: 2573 1156 1042 834 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 2314 2608 Size: 416 53 83 399 24 865 32 294 Cuts at: 2608 3336 4294 442 Size: 728 958 1753 Fragments arranged by size: 1753 958 865 728 416 399 294 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2387 4265 1239 Size: 836 312 1878 2579 Fragments arranged by size: 2579 1878 836 312 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2711 4268 882 Size: 617 1212 1557 2219 Fragments arranged by size: 2219 1557 1212 617 BsaAI yAC'GTr Cuts at: 493 1204 3396 493 Size: 711 2192 2702 Fragments arranged by size: 2702 2192 711 BsaBI GATnn'nnATC Cuts at: 2780 5599 2780 Size: 2819 2786 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2843 4038 4436 4710 4857 5268 2843 Size: 1195 398 274 147 411 3180 Fragments arranged by size: 3180 1195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 1178 Size: 30 165 30 603 30 4747 Fragments arranged by size: 4747 603 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2866 1186 Size: 838 3 839 3925 Fragments arranged by size: 3925 839 838 3 BseYI C'CCAG_C Cuts at: 2032 2747 4808 5304 2032 Size: 715 2061 496 2333 Fragments arranged by size: 2333 2061 715 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 3943 Fragments arranged by size: 3943 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 3666 3751 4822 2262 Size: 1404 85 1071 3045 Fragments arranged by size: 3045 1404 1071 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2832 2924 3666 3751 4822 1391 Size: 399 472 570 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 472 399 92 85 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2712 2804 2960 844 Size: 1868 92 156 3489 Fragments arranged by size: 3489 1868 156 92 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 2669 4100 4274 65 Size: 1183 1213 208 1431 174 1396 Fragments arranged by size: 1431 1396 1213 1183 208 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4249 4436 1700 Size: 110 2439 187 2869 Fragments arranged by size: 2869 2439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 2113 1184 Size: 929 4676 BstYI r'GATC_y Cuts at: 1972 2520 2743 2935 3302 3687 3704 5156 5242 Size: 548 223 192 367 385 17 1452 86 Cuts at: 5242 5254 5600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 548 385 367 346 223 192 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2833 2833 Size: 5605 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 2434 2467 3121 3915 3935 2434 Size: 33 654 794 20 4104 Fragments arranged by size: 4104 794 654 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2443 2478 529 Size: 35 1879 35 3656 Fragments arranged by size: 3656 1879 35 35 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2844 4612 809 Size: 507 1528 1768 1802 Fragments arranged by size: 1802 1768 1528 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2818 3052 3941 Size: 54 1425 50 275 18 988 234 889 Cuts at: 3941 5570 8 Size: 1629 43 Fragments arranged by size: 1629 1425 988 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 3535 1353 Size: 1356 826 3423 Fragments arranged by size: 3423 1356 826 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2211 2387 3668 4265 5052 5320 Size: 393 443 136 176 1281 597 787 268 Cuts at: 5320 1239 Size: 1524 Fragments arranged by size: 1524 1281 787 597 443 393 268 176 136 EcoO109I rG'GnC_Cy Cuts at: 2798 2828 2829 2837 2798 Size: 30 1 8 5566 Fragments arranged by size: 5566 30 8 1 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2431 2492 2431 Size: 61 5544 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 4752 1678 Size: 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2006 2035 2038 4246 Size: 32 135 32 431 3 29 3 2208 Cuts at: 4246 4278 4320 4352 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2208 431 135 42 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4719 4903 1305 Size: 3414 184 2007 Fragments arranged by size: 3414 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2820 10 Size: 54 1425 343 988 2795 Fragments arranged by size: 2795 1425 988 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 3922 4081 518 Size: 648 2397 359 159 2042 Fragments arranged by size: 2397 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3021 3698 4846 5091 1606 Size: 1415 677 1148 245 2120 Fragments arranged by size: 2120 1415 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 NdeI CA'TA_TG Cuts at: 387 2148 387 Size: 1761 3844 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2677 2963 4508 1132 Size: 97 1448 286 1545 2229 Fragments arranged by size: 2229 1545 1448 286 97 PasI CC'CwG_GG Cuts at: 1348 2068 1348 Size: 720 4885 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 2664 1108 Size: 1556 4049 PfoI T'CCnGG_A Cuts at: 2529 2529 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2510 2542 3674 3706 5187 5219 2510 Size: 32 1132 32 1481 32 2896 Fragments arranged by size: 2896 1481 1132 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2837 2837 Size: 5605 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PspOMI G'GGCC_C Cuts at: 2828 2828 Size: 5605 PstI C_TGCA'G Cuts at: 830 2042 2815 2957 830 Size: 1212 773 142 3478 Fragments arranged by size: 3478 1212 773 142 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SbfI CC_TGCA'GG Cuts at: 2815 2957 2815 Size: 142 5463 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2038 2811 2953 4088 4769 4960 826 Size: 219 993 773 142 1135 681 191 1471 Fragments arranged by size: 1471 1135 993 773 681 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2028 2932 1872 Size: 156 904 4545 Fragments arranged by size: 4545 904 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2004 3044 513 Size: 552 939 1040 3074 Fragments arranged by size: 3074 1040 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2333 2365 2612 2735 3632 3649 3802 Size: 1255 753 32 247 123 897 17 153 Cuts at: 3802 3987 4432 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 897 753 445 247 185 153 123 32 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2026 2930 1870 Size: 156 904 4545 Fragments arranged by size: 4545 904 156 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI