Restriction map for pFN21AB6384
 (Circular) (Six-base) MAPSORT of: pFN21AB6384.seq  Check: 5143  from: 1  to: 5605



pFN21AB6384  5605 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2804    2804 
   Size:       5605
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3415     278 
   Size:         53      83     186     936    1879    2468

  Fragments arranged by size: 

                2468    1879     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2481    2948    1937 
   Size:        544     467    4594

  Fragments arranged by size: 

                4594     544     467
 
Acc65I G'GTAC_C

Cuts at:   2926    2926 
   Size:       5605
 
AclI AA'CG_TT

Cuts at:   1563    3732    4105    1563 
   Size:       2169     373    3063

  Fragments arranged by size: 

                3063    2169     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2211    3668    5052    5320    1632 
   Size:        579    1457    1384     268    1917

  Fragments arranged by size: 

                1917    1457    1384     579     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5408
 
AflIII A'CryG_T

Cuts at:   1203    4504    5481    1203 
   Size:       3301     977    1327

  Fragments arranged by size: 

                3301    1327     977
 
AgeI A'CCGG_T

Cuts at:   4436    4436 
   Size:       5605
 
AhdI GACnn_n'nnGTC

Cuts at:   4334    4334 
   Size:       5605
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5605
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5605
 
AlwNI CAG_nnn'CTG

Cuts at:   3348    4920    3348 
   Size:       1572    4033
 
ApaI G_GGCC'C

Cuts at:   2832    2832 
   Size:       5605
 
ApaLI G'TGCA_C

Cuts at:   3662    4818    3662 
   Size:       1156    4449
 
ApoI r'AATT_y

Cuts at:   1493    2914    3140    3292    5401    1493 
   Size:       1421     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1421     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4159     160 
   Size:        872    3127    1606

  Fragments arranged by size: 

                3127    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5605
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2026    2930    1870 
   Size:         73      83     904    4545

  Fragments arranged by size: 

                4545     904      83      73
 
BamHI G'GATC_C

Cuts at:   2935    3302    2935 
   Size:        367    5238
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2926    4381     618 
   Size:        325     217     627    1139    1455    1842

  Fragments arranged by size: 

                1842    1455    1139     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2832    2924    1391 
   Size:       1441      92    4072

  Fragments arranged by size: 

                4072    1441      92
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2316    2517     928 
   Size:        971     417     201    4016

  Fragments arranged by size: 

                4016     971     417     201
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3796    3830    1903 
   Size:         34    1859      34    3678

  Fragments arranged by size: 

                3678    1859      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3497    4707    3497 
   Size:       1210    4395
 
BclI T'GATC_A

Cuts at:   1548    2395    1548 
   Size:        847    4758
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2617    4216     136 
   Size:        107     122      71     631     605     945    1599    1525

  Fragments arranged by size: 

                1599    1525     945     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2520    5600    2520 
   Size:       3080    2525
 
BlpI GC'TnA_GC

Cuts at:   3022    3022 
   Size:       5605
 
Bme1580I G_kGCm'C

Cuts at:   1790    2832    3666    4822    1790 
   Size:       1042     834    1156    2573

  Fragments arranged by size: 

                2573    1156    1042     834
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5605
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2282    2314    2608 
   Size:        416      53      83     399      24     865      32     294

Cuts at:   2608    3336    4294     442 
   Size:        728     958    1753

  Fragments arranged by size: 

                1753     958     865     728     416     399     294      83
                  53      32      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5605
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2740    2740 
   Size:       5605
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2075    2387    4265    1239 
   Size:        836     312    1878    2579

  Fragments arranged by size: 

                2579    1878     836     312
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3730    4595    4893    3730 
   Size:        865     298    4442

  Fragments arranged by size: 

                4442     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2711    4268     882 
   Size:        617    1212    1557    2219

  Fragments arranged by size: 

                2219    1557    1212     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3396     493 
   Size:        711    2192    2702

  Fragments arranged by size: 

                2702    2192     711
 
BsaBI GATnn'nnATC

Cuts at:   2780    5599    2780 
   Size:       2819    2786
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3412    3794    4429     275 
   Size:         53      83     186     936    1879     382     635    1451

  Fragments arranged by size: 

                1879    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2843    4038    4436    4710    4857    5268    2843 
   Size:       1195     398     274     147     411    3180

  Fragments arranged by size: 

                3180    1195     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2006    2036    1178 
   Size:         30     165      30     603      30    4747

  Fragments arranged by size: 

                4747     603     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2024    2027    2866    1186 
   Size:        838       3     839    3925

  Fragments arranged by size: 

                3925     839     838       3
 
BseYI C'CCAG_C

Cuts at:   2032    2747    4808    5304    2032 
   Size:        715    2061     496    2333

  Fragments arranged by size: 

                2333    2061     715     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2825    1163 
   Size:        573    1089    3943

  Fragments arranged by size: 

                3943    1089     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3055    3816    4844    1540 
   Size:        275     186    1054     761    1028    2301

  Fragments arranged by size: 

                2301    1054    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2262    3666    3751    4822    2262 
   Size:       1404      85    1071    3045

  Fragments arranged by size: 

                3045    1404    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3116    3209    3116 
   Size:         93    5512
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2262    2832    2924    3666    3751    4822    1391 
   Size:        399     472     570      92     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     570     472     399      92      85
            
 
BspEI T'CCGG_A

Cuts at:   5268    5268 
   Size:       5605
 
BspHI T'CATG_A

Cuts at:   3494    5224    3494 
   Size:       1730    3875
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2712    2804    2960     844 
   Size:       1868      92     156    3489

  Fragments arranged by size: 

                3489    1868     156      92
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3492    1394 
   Size:        429    1669    3507

  Fragments arranged by size: 

                3507    1669     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2461    2669    4100    4274      65 
   Size:       1183    1213     208    1431     174    1396

  Fragments arranged by size: 

                1431    1396    1213    1183     208     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4249    4436    1700 
   Size:        110    2439     187    2869

  Fragments arranged by size: 

                2869    2439     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5605
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5605
 
BssSI C'ACGA_G

Cuts at:   3665    4677    3665 
   Size:       1012    4593
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5605
 
BstBI TT'CG_AA

Cuts at:   4409    4409 
   Size:       5605
 
BstEII G'GTnAC_C

Cuts at:   1184    2113    1184 
   Size:        929    4676
 
BstYI r'GATC_y

Cuts at:   1972    2520    2743    2935    3302    3687    3704    5156    5242 
   Size:        548     223     192     367     385      17    1452      86

Cuts at:   5242    5254    5600    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     548     385     367     346     223     192
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2833    2833 
   Size:       5605
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4114

  Fragments arranged by size: 

                4114     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4866

  Fragments arranged by size: 

                4866     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2434    2467    3121    3915    3935    2434 
   Size:         33     654     794      20    4104

  Fragments arranged by size: 

                4104     794     654      33      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5605
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2443    2478     529 
   Size:         35    1879      35    3656

  Fragments arranged by size: 

                3656    1879      35      35
 
DraI TTT'AAA

Cuts at:   2909    2909 
   Size:       5605
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2844    4612     809 
   Size:        507    1528    1768    1802

  Fragments arranged by size: 

                1802    1768    1528     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2818    3052    3941 
   Size:         54    1425      50     275      18     988     234     889

Cuts at:   3941    5570       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     988     889     275     234      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3052    1537 
   Size:        275    1240    4090

  Fragments arranged by size: 

                4090    1240     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2709    3535    1353 
   Size:       1356     826    3423

  Fragments arranged by size: 

                3423    1356     826
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4192    4566    4712     387 
   Size:       1064     138    2603     374     146    1280

  Fragments arranged by size: 

                2603    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2075    2211    2387    3668    4265    5052    5320 
   Size:        393     443     136     176    1281     597     787     268

Cuts at:   5320    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1281     787     597     443     393     268     176
                 136
 
EcoO109I rG'GnC_Cy

Cuts at:   2798    2828    2829    2837    2798 
   Size:         30       1       8    5566

  Fragments arranged by size: 

                5566      30       8       1
 
EcoRI G'AATT_C

Cuts at:   2914    3292    2914 
   Size:        378    5227
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2431    2492    2431 
   Size:         61    5544
 
FspI TGC'GCA

Cuts at:   4111    4111 
   Size:       5605
 
HaeII r_GCGC'y

Cuts at:   1678    4752    1678 
   Size:       3074    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2003    2006    2035    2038    4246 
   Size:         32     135      32     431       3      29       3    2208

Cuts at:   4246    4278    4320    4352    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2208     431     135      42      32      32      32
                  32      29       3       3
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2949    3195     669 
   Size:        988     281    1011     246    3079

  Fragments arranged by size: 

                3079    1011     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5605
 
HpaI GTT'AAC

Cuts at:   3195    3195 
   Size:       5605
 
KpnI G_GTAC'C

Cuts at:   2930    2930 
   Size:       5605
 
MfeI C'AATT_G

Cuts at:   3204    3204 
   Size:       5605
 
MluI A'CGCG_T

Cuts at:   5481    5481 
   Size:       5605
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4719    4903    1305 
   Size:       3414     184    2007

  Fragments arranged by size: 

                3414    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2820      10 
   Size:         54    1425     343     988    2795

  Fragments arranged by size: 

                2795    1425     988     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3563    3922    4081     518 
   Size:        648    2397     359     159    2042

  Fragments arranged by size: 

                2397    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3021    3698    4846    5091    1606 
   Size:       1415     677    1148     245    2120

  Fragments arranged by size: 

                2120    1415    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4114

  Fragments arranged by size: 

                4114     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2148     387 
   Size:       1761    3844
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5605
 
NotI GC'GGCC_GC

Cuts at:   3052    3052 
   Size:       5605
 
NspI r_CATG'y

Cuts at:   1132    1229    2677    2963    4508    1132 
   Size:         97    1448     286    1545    2229

  Fragments arranged by size: 

                2229    1545    1448     286      97
 
PasI CC'CwG_GG

Cuts at:   1348    2068    1348 
   Size:        720    4885
 
PciI A'CATG_T

Cuts at:   4504    4504 
   Size:       5605
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2664    1108 
   Size:       1556    4049
 
PfoI T'CCnGG_A

Cuts at:   2529    2529 
   Size:       5605
 
PmeI GTTT'AAAC

Cuts at:   2909    2909 
   Size:       5605
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2510    2542    3674    3706    5187    5219    2510 
   Size:         32    1132      32    1481      32    2896

  Fragments arranged by size: 

                2896    1481    1132      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2837    2837 
   Size:       5605
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5605
 
PsiI TTA'TAA

Cuts at:   3175    3175 
   Size:       5605
 
PspOMI G'GGCC_C

Cuts at:   2828    2828 
   Size:       5605
 
PstI C_TGCA'G

Cuts at:    830    2042    2815    2957     830 
   Size:       1212     773     142    3478

  Fragments arranged by size: 

                3478    1212     773     142
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5605
 
SalI G'TCGA_C

Cuts at:   1936    2947    1936 
   Size:       1011    4594
 
SbfI CC_TGCA'GG

Cuts at:   2815    2957    2815 
   Size:        142    5463
 
ScaI AGT'ACT

Cuts at:   3853    5565    3853 
   Size:       1712    3893
 
SfcI C'TryA_G

Cuts at:    826    1045    2038    2811    2953    4088    4769    4960     826 
   Size:        219     993     773     142    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135     993     773     681     219     191     142
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5605
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5605
 
SmaI CCC'GGG

Cuts at:   1872    2028    2932    1872 
   Size:        156     904    4545

  Fragments arranged by size: 

                4545     904     156
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3709    4610    4872     820 
   Size:        197     926    1766     901     262    1553

  Fragments arranged by size: 

                1766    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3396     493 
   Size:       2903    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5605
 
SphI G_CATG'C

Cuts at:   1132    2963    1132 
   Size:       1831    3774
 
SspI AAT'ATT

Cuts at:      5      52    3529       5 
   Size:         47    3477    2081

  Fragments arranged by size: 

                3477    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5605
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3044     513 
   Size:        552     939    1040    3074

  Fragments arranged by size: 

                3074    1040     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2333    2365    2612    2735    3632    3649    3802 
   Size:       1255     753      32     247     123     897      17     153

Cuts at:   3802    3987    4432     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                1498    1255     897     753     445     247     185     153
                 123      32      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3851    5563      96 
   Size:        274      80      33      51    1443    1874    1712     138

  Fragments arranged by size: 

                1874    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5565    5597    5565 
   Size:         32    5573
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5605
 
XbaI T'CTAG_A

Cuts at:   2941    2941 
   Size:       5605
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5605
 
XmaI C'CCGG_G

Cuts at:   1870    2026    2930    1870 
   Size:        156     904    4545

  Fragments arranged by size: 

                4545     904     156
 
XmnI GAAnn'nnTTC

Cuts at:   3734    3734 
   Size:       5605
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3413     276 
   Size:         53      83     186     936    1879    2468

  Fragments arranged by size: 

                2468    1879     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI    EcoNI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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