Restriction map for
pFN21AB6821
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DpnII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MscI MslI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 164 TCCGGA T^CCGGA
AciI 66 CCGC CCGC(-3/-1)
AfaI 448 GTAC GT^AC
ApaI 31 GGGCCC GGGCC^C
AvaII 152 GGACC G^G[AT]CC
BbvI 172 GCAGC GCAGC(8/12)
BcgI 53 GCATGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 164 TCCGGA [AT]^CCGG[AT]
BseAI 164 TCCGGA T^CCGGA
BsmI 160 GCATTC GAATGC(1/-1)
BspEI 164 TCCGGA T^CCGGA
BssKI 43 CCTGG ^CC[ACGT]GG
BstAPI 68 GCAACGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 43 CCTGG CC^[AT]GG
Cac8I 415 GCAGGC GC[ACGT]^[ACGT]GC
DraI 118 TTTAAA TTT^AAA
DraII 30 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoNI 409 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 30 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 43 CCTGG ^CC[AT]GG
EcoRV 367 GATATC GAT^ATC
Fnu4HI 172 GCAGC GC^[ACGT]GC
HaeII 305 AGCGCC [GA]GCGC^[CT]
HaeIII 32 GGCC GG^CC
HapII 165 CCGG C^CGG
HpaII 165 CCGG C^CGG
ItaI 172 GCAGC GC^[ACGT]GC
MaeII 219 ACGT A^CGT
MaeIII 456 GTTAC ^GT[ACGT]AC
MroI 164 TCCGGA T^CCGGA
MseI 119 TTAA T^TAA
MspA1I 173 CAGCTG C[AC]G^C[GT]G
MspI 165 CCGG C^CGG
MvaI 43 CCTGG CC^[AT]GG
PvuII 173 CAGCTG CAG^CTG
RsaI 448 GTAC GT^AC
ScrFI 43 CCTGG CC^[ACGT]GG
SfcI 352 CTACAG C^T[GA][CT]AG
TaqI 8 TCGA T^CGA
Tru9I 119 TTAA T^TAA
TseI 172 GCAGC G^C[AT]GC
TspRI 191 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 8 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 208 GAATTT [GA]^AATT[CT]
316 AAATTT
ApoI 208 GAATTT [GA]^AATT[CT]
316 AAATTT
BanII 31 GGGCCC G[GA]GC[CT]^C
404 GAGCCC
BmyI 31 GGGCCC G[AGT]GC[ACT]^C
404 GAGCCC
BsiYI 43 CCTGGACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
409 CCTCTTGCAGG
BslI 43 CCTGGACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
409 CCTCTTGCAGG
Bsp1286I 31 GGGCCC G[AGT]GC[ACT]^C
404 GAGCCC
BstF5I 390 CATCC GGATG(2/0)
465 GGATG
CfoI 129 GCGC GCG^C
306 GCGC
EarI 279 GAAGAG CTCTTC(1/4)
436 GAAGAG
FokI 390 CATCC GGATG(9/13)
465 GGATG
HhaI 129 GCGC GCG^C
306 GCGC
HinP1I 129 GCGC G^CGC
306 GCGC
Ksp632I 279 GAAGAG CTCTTC(1/4)
436 GAAGAG
NlaIII 54 CATG CATG^
474 CATG
NlaIV 30 GGGGCC GG[ACGT]^[ACGT]CC
31 GGGCCC
SfaNI 271 GATGC GCATC(5/9)
481 GATGC
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI Cfr13I MboII MnlI MwoI
Sau96I Tsp509I