################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AvaII 45 GGACC G^G[AT]CC BanI 4 GGTGCC G^G[CT][GA]CC BcgI 383 CGAAGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 102 CCCGG CC^[GC]GG BmyI 5 GTGCCC G[AGT]GC[ACT]^C BsaWI 244 TCCGGT [AT]^CCGG[AT] BsgI 96 CTGCAC GTGCAG(16/14) BsmI 237 GCATTC GAATGC(1/-1) Bsp1286I 5 GTGCCC G[AGT]GC[ACT]^C BsrBI 106 GAGCGG CCGCTC(-3/-3) BstNI 26 CCTGG CC^[AT]GG DdeI 143 CTGAG C^T[ACGT]AG DsaI 419 CCATGG C^C[GA][CT]GG EaeI 113 TGGCCG [CT]^GGCC[GA] EcoRII 26 CCTGG ^CC[AT]GG HphI 295 GGTGA GGTGA(8/7) MaeII 13 ACGT A^CGT MseI 286 TTAA T^TAA MspA1I 409 CAGCTG C[AC]G^C[GT]G MvaI 26 CCTGG CC^[AT]GG MwoI 89 GCTGCGCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 102 CCCGG CC^[GC]GG NcoI 419 CCATGG C^CATGG NlaIII 420 CATG CATG^ PvuII 409 CAGCTG CAG^CTG SfaNI 53 GCATC GCATC(5/9) SmlI 40 CTCAAG C^T[CT][GA]AG TaqI 404 TCGA T^CGA Tru9I 286 TTAA T^TAA TthHB8I 404 TCGA T^CGA XcmI 401 CCATCGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 51 CCGC CCGC(-3/-1) 108 GCGG AcsI 298 GAATTT [GA]^AATT[CT] 367 GAATTT AluI 188 AGCT AG^CT 410 AGCT ApoI 298 GAATTT [GA]^AATT[CT] 367 GAATTT BssKI 26 CCTGG ^CC[ACGT]GG 102 CCCGG BstF5I 129 CATCC GGATG(2/0) 110 GGATG EcoT14I 291 CCTTGG C^C[AT][AT]GG 419 CCATGG FokI 129 CATCC GGATG(9/13) 110 GGATG HapII 103 CCGG C^CGG 245 CCGG HpaII 103 CCGG C^CGG 245 CCGG MspI 103 CCGG C^CGG 245 CCGG NlaIV 4 GGTGCC GG[ACGT]^[ACGT]CC 201 GGAGCC ScrFI 26 CCTGG CC^[ACGT]GG 102 CCCGG StyI 291 CCTTGG C^C[AT][AT]GG 419 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### BbvI BsaJI BsiYI BslI BsrI Cac8I CfoI Cfr13I Fnu4HI HaeIII HhaI HinP1I ItaI MboII MnlI Sau96I TseI Tsp509I TspRI