Restriction map for
pFN21AE0424
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 952 GGTACC G^GTACC
AccI 406 GTCGAC GT^[AC][GT]AC
AccII 41 CGCG CG^CG
Asp718I 952 GGTACC G^GTACC
AspHI 621 GAGCAC G[AT]GC[AT]^C
BamHI 797 GGATCC G^GATCC
BbvCI 1341 CCTCAGC CCTCAGC(-5/-2)
BcgI 408 CGACTGGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 700 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 288 GCTGAGC GC^T[ACGT]AGC
BpmI 244 CTGGAG CTGGAG(16/14)
Bpu1102I 288 GCTGAGC GC^T[ACGT]AGC
BsiEI 179 CGGCCG CG[GA][CT]^CG
BsiHKAI 621 GAGCAC G[AT]GC[AT]^C
BsmAI 1145 GTCTC GTCTC(1/5)
BsmI 363 GAATGC GAATGC(1/-1)
BspHI 189 TCATGA T^CATGA
BsrBI 788 CCGCTC CCGCTC(-3/-3)
BsrDI 1046 GCAATG GCAATG(2/0)
BsrFI 78 GCCGGT [GA]^CCGG[CT]
BssSI 1150 CTCGTG CACGAG(-5/-1)
BstAPI 1179 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 41 CGCG CG^CG
BstXI 1041 CCACAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 288 GCTGAGC GC^T[ACGT]AGC
Cfr10I 78 GCCGGT [GA]^CCGG[CT]
CpoI 668 CGGTCCG CG^G[AT]CCG
EagI 179 CGGCCG C^GGCCG
EarI 1309 CTCTTC CTCTTC(1/4)
EclXI 179 CGGCCG C^GGCCG
Eco52I 179 CGGCCG C^GGCCG
EcoNI 914 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 406 GTCGAC GT[CT]^[GA]AC
HindII 406 GTCGAC GT[CT]^[GA]AC
KpnI 952 GGTACC GGTAC^C
Ksp632I 1309 CTCTTC CTCTTC(1/4)
KspI 40 CCGCGG CCGC^GG
MaeII 1217 ACGT A^CGT
MvnI 41 CGCG CG^CG
NdeI 311 CATATG CA^TATG
PaeR7I 23 CTCGAG C^TCGAG
PvuII 727 CAGCTG CAG^CTG
RcaI 189 TCATGA T^CATGA
RsrII 668 CGGTCCG CG^G[AT]CCG
SacII 40 CCGCGG CCGC^GG
SalI 406 GTCGAC G^TCGAC
SapI 1308 GCTCTTC GCTCTTC(1/4)
SphI 65 GCATGC GCATG^C
XhoI 23 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 831 GTAC GT^AC
953 GTAC
AvaI 23 CTCGAG C^[CT]CG[GA]G
454 CTCGGG
BalI 482 TGGCCA TGG^CCA
980 TGGCCA
BanI 570 GGCACC G^G[CT][GA]CC
952 GGTACC
BfaI 358 CTAG C^TAG
718 CTAG
BseRI 384 CTCCTC GAGGAG(10/8)
1147 CTCCTC
BsgI 1330 CTGCAC GTGCAG(16/14)
874 GTGCAG
BsoBI 23 CTCGAG C^[CT]CG[GA]G
454 CTCGGG
MaeI 358 CTAG C^TAG
718 CTAG
MluNI 482 TGGCCA TGG^CCA
980 TGGCCA
MscI 482 TGGCCA TGG^CCA
980 TGGCCA
MslI 1042 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1275 CATCTGGATG
NspI 65 GCATGC [GA]CATG^[CT]
471 GCATGT
PstI 277 CTGCAG CTGCA^G
442 CTGCAG
RsaI 831 GTAC GT^AC
953 GTAC
SbfI 276 CCTGCAGG CCTGCA^GG
441 CCTGCAGG
SfcI 277 CTGCAG C^T[GA][CT]AG
442 CTGCAG
Sse8387I 276 CCTGCAGG CCTGCA^GG
441 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII