(Circular) (Six-base) MAPSORT of: pFN21AE0424.seq Check: 2743 from: 1 to: 6115 pFN21AE0424 6115 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 3458 1937 Size: 475 1046 4594 Fragments arranged by size: 4594 1046 475 Acc65I G'GTAC_C Cuts at: 2957 3436 2957 Size: 479 5636 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 3179 4178 5562 5830 1632 Size: 893 654 999 1384 268 1917 Fragments arranged by size: 1917 1384 999 893 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6115 AlwNI CAG_nnn'CTG Cuts at: 2623 3008 3255 3858 5430 2623 Size: 385 247 603 1572 3308 Fragments arranged by size: 3308 1572 603 385 247 ApaI G_GGCC'C Cuts at: 2818 3096 3389 2818 Size: 278 293 5544 Fragments arranged by size: 5544 293 278 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2459 3440 1870 Size: 73 85 431 981 4545 Fragments arranged by size: 4545 981 431 85 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2949 2982 2949 Size: 33 6082 BamHI G'GATC_C Cuts at: 2802 3445 3812 2802 Size: 643 367 5105 Fragments arranged by size: 5105 643 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2575 2957 3436 4891 618 Size: 325 217 627 788 382 479 1455 1842 Fragments arranged by size: 1842 1455 788 627 479 382 325 217 BanII G_rGCy'C Cuts at: 1391 2179 2818 3096 3389 3434 1391 Size: 788 639 278 293 45 4072 Fragments arranged by size: 4072 788 639 293 278 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5144 BbvCI CC'TCA_GC Cuts at: 3347 3347 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2402 2436 4306 4340 1903 Size: 34 465 34 1870 34 3678 Fragments arranged by size: 3678 1870 465 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2711 4726 Size: 107 122 71 631 605 337 702 2015 Cuts at: 4726 136 Size: 1525 Fragments arranged by size: 2015 1525 702 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 2294 3532 2294 Size: 1238 4877 Bme1580I G_kGCm'C Cuts at: 1790 2818 3096 3389 4176 5332 1790 Size: 1028 278 293 787 1156 2573 Fragments arranged by size: 2573 1156 1028 787 293 278 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2996 3846 4804 Size: 416 53 83 399 24 1579 850 958 Cuts at: 4804 442 Size: 1753 Fragments arranged by size: 1753 1579 958 850 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3256 3256 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 4775 1239 Size: 1031 2505 2579 Fragments arranged by size: 2579 2505 1031 Bpu10I CC'TnA_GC Cuts at: 2624 3134 3347 2624 Size: 510 213 5392 Fragments arranged by size: 5392 510 213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 3338 4240 5105 5403 2618 Size: 720 902 865 298 3330 Fragments arranged by size: 3330 902 865 720 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4778 882 Size: 617 3279 2219 Fragments arranged by size: 3279 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3906 493 Size: 711 2702 2702 Fragments arranged by size: 2702 2702 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3922 4304 4939 275 Size: 53 83 186 936 2389 382 635 1451 Fragments arranged by size: 2389 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4548 4946 5220 5367 5778 4548 Size: 398 274 147 411 4885 Fragments arranged by size: 4885 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5890 Fragments arranged by size: 5890 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2380 3143 1186 Size: 1194 763 4158 Fragments arranged by size: 4158 1194 763 BseYI C'CCAG_C Cuts at: 2254 3095 5318 5814 2254 Size: 841 2223 496 2555 Fragments arranged by size: 2555 2223 841 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2900 3320 1163 Size: 573 1164 420 3958 Fragments arranged by size: 3958 1164 573 420 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2187 3565 4326 5354 1540 Size: 275 186 186 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1028 761 275 186 186 BsiHKAI G_wGCw'C Cuts at: 2630 4176 4261 5332 2630 Size: 1546 85 1071 3413 Fragments arranged by size: 3413 1546 1071 85 BsmI GAATG_Cn' Cuts at: 2374 3626 3719 2374 Size: 1252 93 4770 Fragments arranged by size: 4770 1252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2630 2818 3096 3389 3434 4176 Size: 399 389 451 188 278 293 45 742 Cuts at: 4176 4261 5332 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 399 389 293 278 188 85 45 BspEI T'CCGG_A Cuts at: 5778 5778 Size: 6115 BspHI T'CATG_A Cuts at: 2194 4004 5734 2194 Size: 1810 1730 2575 Fragments arranged by size: 2575 1810 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2125 2454 2608 3470 844 Size: 1281 329 154 862 3489 Fragments arranged by size: 3489 1281 862 329 154 BsrBI CCG'CTC Cuts at: 1394 1823 2795 4002 1394 Size: 429 972 1207 3507 Fragments arranged by size: 3507 1207 972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3058 4610 4784 65 Size: 1183 1810 1552 174 1396 Fragments arranged by size: 1810 1552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 4759 4946 1700 Size: 110 273 2676 187 2869 Fragments arranged by size: 2869 2676 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 3155 4175 5187 3155 Size: 1020 1012 4083 Fragments arranged by size: 4083 1020 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3190 794 Size: 2396 3719 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstXI CCAn_nnnn'nTGG Cuts at: 3053 3053 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2239 2802 3207 3445 3812 4197 4214 5666 Size: 267 563 405 238 367 385 17 1452 Cuts at: 5666 5752 5764 6110 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 563 405 385 367 346 267 238 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2045 2075 2706 3027 3360 513 Size: 552 951 29 30 631 321 333 3268 Fragments arranged by size: 3268 951 631 552 333 321 30 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3631 4425 4445 3112 Size: 519 794 20 4782 Fragments arranged by size: 4782 794 519 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2184 2487 Size: 54 1425 50 275 18 183 171 303 Cuts at: 2487 2712 2985 3562 4451 6080 8 Size: 225 273 577 889 1629 43 Fragments arranged by size: 1629 1425 889 577 303 275 273 225 183 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2184 3562 1537 Size: 275 372 1378 4090 Fragments arranged by size: 4090 1378 372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3320 4045 1353 Size: 1967 725 3423 Fragments arranged by size: 3423 1967 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2141 2666 4702 5076 5222 387 Size: 1064 138 552 525 2036 374 146 1280 Fragments arranged by size: 2036 1280 1064 552 525 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2525 3179 4178 4775 5562 5830 Size: 393 638 255 654 999 597 787 268 Cuts at: 5830 1239 Size: 1524 Fragments arranged by size: 1524 999 787 654 638 597 393 268 255 EcoNI CCTnn'n_nnAGG Cuts at: 2923 2923 Size: 6115 EcoO109I rG'GnC_Cy Cuts at: 2137 2258 2620 2854 3092 3385 3386 2137 Size: 121 362 234 238 293 1 4866 Fragments arranged by size: 4866 362 293 238 234 121 1 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 2063 2352 3037 5262 1678 Size: 385 289 685 2225 2531 Fragments arranged by size: 2531 2225 685 385 289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2755 2787 3349 3381 4756 Size: 32 135 32 1183 32 562 32 1375 Cuts at: 4756 4788 4830 4862 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1375 1183 562 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2413 3459 3705 669 Size: 988 281 475 1046 246 3079 Fragments arranged by size: 3079 1046 988 475 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6115 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KpnI G_GTAC'C Cuts at: 2961 3440 2961 Size: 479 5636 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5229 5413 1305 Size: 3924 184 2007 Fragments arranged by size: 3924 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2489 2987 10 Size: 54 1425 343 657 498 3138 Fragments arranged by size: 3138 1425 657 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3051 3284 4073 4432 4591 518 Size: 648 1885 233 789 359 159 2042 Fragments arranged by size: 2042 1885 789 648 359 233 159 MspA1I CmG'CkG Cuts at: 1606 2047 2123 2143 2660 2672 2734 3531 4208 Size: 441 76 20 517 12 62 797 677 Cuts at: 4208 5356 5601 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 797 677 517 441 245 76 62 20 12 NcoI C'CATG_G Cuts at: 513 1065 2016 3027 3360 513 Size: 552 951 1011 333 3268 Fragments arranged by size: 3268 1011 951 552 333 NdeI CA'TA_TG Cuts at: 387 2317 387 Size: 1930 4185 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 2074 2480 3473 5018 1132 Size: 97 845 406 993 1545 2229 Fragments arranged by size: 2229 1545 993 845 406 97 PasI CC'CwG_GG Cuts at: 1348 2580 1348 Size: 1232 4883 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 3412 1108 Size: 2304 3811 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2258 2620 2854 2137 Size: 121 362 234 5398 Fragments arranged by size: 5398 362 234 121 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 2814 3092 3385 2814 Size: 278 293 5544 Fragments arranged by size: 5544 293 278 PspXI vC'TCGA_Gb Cuts at: 2028 2028 Size: 6115 PstI C_TGCA'G Cuts at: 830 2286 2451 3467 830 Size: 1456 165 1016 3478 Fragments arranged by size: 3478 1456 1016 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 2734 2734 Size: 6115 RsrII CG'GwC_CG Cuts at: 2674 2674 Size: 6115 SacII CC_GC'GG Cuts at: 2048 2048 Size: 6115 SalI G'TCGA_C Cuts at: 1936 2411 3457 1936 Size: 475 1046 4594 Fragments arranged by size: 4594 1046 475 SanDI GG'GwC_CC Cuts at: 2258 2258 Size: 6115 SapI GCTCTTCn'nnn_ Cuts at: 3320 3320 Size: 6115 SbfI CC_TGCA'GG Cuts at: 2286 2451 3467 2286 Size: 165 1016 4934 Fragments arranged by size: 4934 1016 165 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2282 2447 3463 4598 5279 5470 826 Size: 219 1237 165 1016 1135 681 191 1471 Fragments arranged by size: 1471 1237 1135 1016 681 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 3442 1872 Size: 1570 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2028 2633 3353 4219 5120 5382 Size: 197 926 85 605 720 866 901 262 Cuts at: 5382 820 Size: 1553 Fragments arranged by size: 1553 926 901 866 720 605 262 197 85 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 2074 3473 1132 Size: 942 1399 3774 Fragments arranged by size: 3774 1399 942 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 2016 3027 3360 3554 513 Size: 552 951 1011 333 194 3074 Fragments arranged by size: 3074 1011 951 552 333 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2593 4142 4159 4312 4497 4942 325 Size: 1255 1013 1549 17 153 185 445 1498 Fragments arranged by size: 1549 1498 1255 1013 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2748 2780 6075 6107 2748 Size: 32 3295 32 2756 Fragments arranged by size: 3295 2756 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 2028 1943 Size: 85 6030 XmaI C'CCGG_G Cuts at: 1870 3440 1870 Size: 1570 4545 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI SacI SexAI SfiI SfoI SrfI SwaI XcmI