Restriction map for
pFN21AE0424
(Linear) (Six-base) MAPSORT of: KIEE0424.seq Check: 8721 from: 1 to: 1410
KIEE0424.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 407 1410
Size: 407 1003
Acc65I G'GTAC_C
Cuts at: 0 952 1410
Size: 952 458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1174 1410
Size: 520 654 236
Fragments arranged by size:
654 520 236
AlwNI CAG_nnn'CTG
Cuts at: 0 618 1003 1250 1410
Size: 618 385 247 160
Fragments arranged by size:
618 385 247 160
ApaI G_GGCC'C
Cuts at: 0 813 1091 1384 1410
Size: 813 278 293 26
Fragments arranged by size:
813 293 278 26
AvaI C'yCGr_G
Cuts at: 0 23 454 1410
Size: 23 431 956
Fragments arranged by size:
956 431 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 944 977 1410
Size: 944 33 433
Fragments arranged by size:
944 433 33
BamHI G'GATC_C
Cuts at: 0 797 1410
Size: 797 613
BanI G'GyrC_C
Cuts at: 0 570 952 1410
Size: 570 382 458
Fragments arranged by size:
570 458 382
BanII G_rGCy'C
Cuts at: 0 174 813 1091 1384 1410
Size: 174 639 278 293 26
Fragments arranged by size:
639 293 278 174 26
BbvCI CC'TCA_GC
Cuts at: 0 1342 1410
Size: 1342 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 431 1410
Size: 397 34 979
Fragments arranged by size:
979 397 34
BglI GCCn_nnn'nGGC
Cuts at: 0 706 1410
Size: 706 704
BlpI GC'TnA_GC
Cuts at: 0 289 1410
Size: 289 1121
Bme1580I G_kGCm'C
Cuts at: 0 813 1091 1384 1410
Size: 813 278 293 26
Fragments arranged by size:
813 293 278 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 991 1410
Size: 991 419
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1251 1410
Size: 1251 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1410
Size: 265 1145
Bpu10I CC'TnA_GC
Cuts at: 0 619 1129 1342 1410
Size: 619 510 213 68
Fragments arranged by size:
619 510 213 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1333 1410
Size: 613 720 77
Fragments arranged by size:
720 613 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 375 1138 1410
Size: 375 763 272
Fragments arranged by size:
763 375 272
BseYI C'CCAG_C
Cuts at: 0 249 1090 1410
Size: 249 841 320
Fragments arranged by size:
841 320 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1315 1410
Size: 895 420 95
Fragments arranged by size:
895 420 95
BsiEI CG_ry'CG
Cuts at: 0 182 1410
Size: 182 1228
BsiHKAI G_wGCw'C
Cuts at: 0 625 1410
Size: 625 785
BsmI GAATG_Cn'
Cuts at: 0 369 1410
Size: 369 1041
Bsp1286I G_dGCh'C
Cuts at: 0 174 625 813 1091 1384 1410
Size: 174 451 188 278 293 26
Fragments arranged by size:
451 293 278 188 174 26
BspHI T'CATG_A
Cuts at: 0 189 1410
Size: 189 1221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 120 449 603 1410
Size: 120 329 154 807
Fragments arranged by size:
807 329 154 120
BsrBI CCG'CTC
Cuts at: 0 790 1410
Size: 790 620
BsrDI GCAATG_nn'
Cuts at: 0 1053 1410
Size: 1053 357
BsrFI r'CCGG_y
Cuts at: 0 78 1410
Size: 78 1332
BssSI C'ACGA_G
Cuts at: 0 1150 1410
Size: 1150 260
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1185 1410
Size: 1185 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1048 1410
Size: 1048 362
BstYI r'GATC_y
Cuts at: 0 234 797 1202 1410
Size: 234 563 405 208
Fragments arranged by size:
563 405 234 208
BtgI C'CryG_G
Cuts at: 0 11 40 70 701 1022 1355 1410
Size: 11 29 30 631 321 333 55
Fragments arranged by size:
631 333 321 55 30 29 11
BtsI GCAGTG_nn'
Cuts at: 0 1107 1410
Size: 1107 303
EaeI y'GGCC_r
Cuts at: 0 8 179 482 707 980 1410
Size: 8 171 303 225 273 430
Fragments arranged by size:
430 303 273 225 171 8
EagI C'GGCC_G
Cuts at: 0 179 1410
Size: 179 1231
EarI CTCTTCn'nnn_
Cuts at: 0 1315 1410
Size: 1315 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 136 661 1410
Size: 136 525 749
Fragments arranged by size:
749 525 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 520 1174 1410
Size: 265 255 654 236
Fragments arranged by size:
654 265 255 236
EcoNI CCTnn'n_nnAGG
Cuts at: 0 918 1410
Size: 918 492
EcoO109I rG'GnC_Cy
Cuts at: 0 132 253 615 849 1087 1380 1381 1410
Size: 132 121 362 234 238 293 1 29
Fragments arranged by size:
362 293 238 234 132 121 29 1
HaeII r_GCGC'y
Cuts at: 0 58 347 1032 1410
Size: 58 289 685 378
Fragments arranged by size:
685 378 289 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 750 782 1344 1376 1410
Size: 750 32 562 32 34
Fragments arranged by size:
750 562 34 32 32
HincII GTy'rAC
Cuts at: 0 408 1410
Size: 408 1002
KpnI G_GTAC'C
Cuts at: 0 956 1410
Size: 956 454
MscI TGG'CCA
Cuts at: 0 484 982 1410
Size: 484 498 428
Fragments arranged by size:
498 484 428
MslI CAynn'nnrTG
Cuts at: 0 1046 1279 1410
Size: 1046 233 131
Fragments arranged by size:
1046 233 131
MspA1I CmG'CkG
Cuts at: 0 42 118 138 655 667 729 1410
Size: 42 76 20 517 12 62 681
Fragments arranged by size:
681 517 76 62 42 20 12
NcoI C'CATG_G
Cuts at: 0 11 1022 1355 1410
Size: 11 1011 333 55
Fragments arranged by size:
1011 333 55 11
NdeI CA'TA_TG
Cuts at: 0 312 1410
Size: 312 1098
NspI r_CATG'y
Cuts at: 0 69 475 1410
Size: 69 406 935
Fragments arranged by size:
935 406 69
PasI CC'CwG_GG
Cuts at: 0 575 1410
Size: 575 835
PpuMI rG'GwC_Cy
Cuts at: 0 132 253 615 849 1410
Size: 132 121 362 234 561
Fragments arranged by size:
561 362 234 132 121
PspOMI G'GGCC_C
Cuts at: 0 809 1087 1380 1410
Size: 809 278 293 30
Fragments arranged by size:
809 293 278 30
PspXI vC'TCGA_Gb
Cuts at: 0 23 1410
Size: 23 1387
PstI C_TGCA'G
Cuts at: 0 281 446 1410
Size: 281 165 964
Fragments arranged by size:
964 281 165
PvuII CAG'CTG
Cuts at: 0 729 1410
Size: 729 681
RsrII CG'GwC_CG
Cuts at: 0 669 1410
Size: 669 741
SacII CC_GC'GG
Cuts at: 0 43 1410
Size: 43 1367
SalI G'TCGA_C
Cuts at: 0 406 1410
Size: 406 1004
SanDI GG'GwC_CC
Cuts at: 0 253 1410
Size: 253 1157
SapI GCTCTTCn'nnn_
Cuts at: 0 1315 1410
Size: 1315 95
SbfI CC_TGCA'GG
Cuts at: 0 281 446 1410
Size: 281 165 964
Fragments arranged by size:
964 281 165
SfcI C'TryA_G
Cuts at: 0 277 442 1410
Size: 277 165 968
Fragments arranged by size:
968 277 165
SmlI C'TyrA_G
Cuts at: 0 23 628 1348 1410
Size: 23 605 720 62
Fragments arranged by size:
720 605 62 23
SphI G_CATG'C
Cuts at: 0 69 1410
Size: 69 1341
StyI C'CwwG_G
Cuts at: 0 11 1022 1355 1410
Size: 11 1011 333 55
Fragments arranged by size:
1011 333 55 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 588 1410
Size: 588 822
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 743 775 1410
Size: 743 32 635
Fragments arranged by size:
743 635 32
XhoI C'TCGA_G
Cuts at: 0 23 1410
Size: 23 1387
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI AvaI BaeI BamHI
BanI BanII BbvCI BcgI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KpnI MscI MslI MspA1I NcoI NdeI NspI PasI
PpuMI PspOMI PspXI PstI PvuII RsrII SacII SalI
SanDI SapI SbfI SfcI SmlI SphI StyI TaqII
TstI XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BbeI BbsI BciVI BclI BfrBI BglII BmgBI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI
BsmBI BspEI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI SacI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XmaI
XmnI ZraI