(Circular) (Six-base) MAPSORT of: pFN21AE0337.seq Check: 5120 from: 1 to: 6232 pFN21AE0337 6232 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 4295 5679 5947 1632 Size: 1739 924 1384 268 1917 Fragments arranged by size: 1917 1739 1384 924 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2739 1184 Size: 1555 4677 AlwNI CAG_nnn'CTG Cuts at: 2485 2536 3245 3975 5547 2485 Size: 51 709 730 1572 3170 Fragments arranged by size: 3170 1572 730 709 51 ApaI G_GGCC'C Cuts at: 2474 3049 2474 Size: 575 5657 ApaLI G'TGCA_C Cuts at: 2215 4289 5445 2215 Size: 2074 1156 3002 Fragments arranged by size: 3002 2074 1156 ApoI r'AATT_y Cuts at: 1493 3541 3767 3919 6028 1493 Size: 2048 226 152 2109 1697 Fragments arranged by size: 2109 2048 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2298 2349 2880 3444 3557 1870 Size: 73 355 51 531 564 113 4545 Fragments arranged by size: 4545 564 531 355 113 73 51 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2130 2287 2382 3553 Size: 325 217 627 230 113 157 95 1171 Cuts at: 3553 5008 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1171 627 325 230 217 157 113 95 BanII G_rGCy'C Cuts at: 1391 2474 3049 3116 3218 3285 3551 1391 Size: 1083 575 67 102 67 266 4072 Fragments arranged by size: 4072 1083 575 266 102 67 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5261 BbvCI CC'TCA_GC Cuts at: 3083 3083 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2871 2905 4423 4457 1903 Size: 34 934 34 1518 34 3678 Fragments arranged by size: 3678 1518 934 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2587 4843 136 Size: 107 122 71 631 605 915 2256 1525 Fragments arranged by size: 2256 1525 915 631 605 122 107 71 BglII A'GATC_T Cuts at: 3468 6227 3468 Size: 2759 3473 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2020 2133 2219 2292 2474 3049 3104 4293 Size: 230 113 86 73 182 575 55 1189 Cuts at: 4293 5449 1790 Size: 1156 2573 Fragments arranged by size: 2573 1189 1156 575 230 182 113 86 73 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3397 3963 4921 Size: 416 53 83 399 24 1980 566 958 Cuts at: 4921 442 Size: 1753 Fragments arranged by size: 1980 1753 958 566 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2375 2375 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 3244 4892 1239 Size: 1097 908 1648 2579 Fragments arranged by size: 2579 1648 1097 908 Bpu10I CC'TnA_GC Cuts at: 3083 3083 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3380 4357 5222 5520 3380 Size: 977 865 298 4092 Fragments arranged by size: 4092 977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2858 2903 4895 882 Size: 617 1359 45 1992 2219 Fragments arranged by size: 2219 1992 1359 617 45 BsaAI yAC'GTr Cuts at: 493 1204 2200 4023 493 Size: 711 996 1823 2702 Fragments arranged by size: 2702 1823 996 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2280 2643 4039 4421 Size: 53 83 186 936 747 363 1396 382 Cuts at: 4421 5056 275 Size: 635 1451 Fragments arranged by size: 1451 1396 936 747 635 382 363 186 83 53 BsaWI w'CCGG_w Cuts at: 2894 4665 5063 5337 5484 5895 2894 Size: 1771 398 274 147 411 3231 Fragments arranged by size: 3231 1771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 2333 1186 Size: 835 312 5085 Fragments arranged by size: 5085 835 312 BseYI C'CCAG_C Cuts at: 2135 2291 2646 3041 3097 3334 3431 3499 5435 Size: 156 355 395 56 237 97 68 1936 Cuts at: 5435 5931 2135 Size: 496 2436 Fragments arranged by size: 2436 1936 496 395 355 237 156 97 68 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3472 1163 Size: 573 1736 3923 Fragments arranged by size: 3923 1736 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2248 3682 4443 5471 1540 Size: 275 186 247 1434 761 1028 2301 Fragments arranged by size: 2301 1434 1028 761 275 247 186 BsiHKAI G_wGCw'C Cuts at: 2219 4293 4378 5449 2219 Size: 2074 85 1071 3002 Fragments arranged by size: 3002 2074 1071 85 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 BsmBI CGTCTCn'nnnn_ Cuts at: 2822 2822 Size: 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2133 2219 2292 2474 3049 3104 Size: 399 230 113 86 73 182 575 55 Cuts at: 3104 3116 3218 3285 3551 4293 4378 5449 1391 Size: 12 102 67 266 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 399 266 230 182 113 102 86 85 73 67 55 12 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 3277 4121 5851 3277 Size: 844 1730 3658 Fragments arranged by size: 3658 1730 844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2498 3024 3339 3587 844 Size: 1654 526 315 248 3489 Fragments arranged by size: 3489 1654 526 315 248 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3511 4727 4901 65 Size: 1183 2263 1216 174 1396 Fragments arranged by size: 2263 1396 1216 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 2247 2372 2500 4876 5063 1700 Size: 110 433 4 125 128 2376 187 2869 Fragments arranged by size: 2869 2376 433 187 128 125 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 2993 4292 5304 2993 Size: 1299 1012 3921 Fragments arranged by size: 3921 1299 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3245 794 Size: 2451 3781 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 2323 1184 Size: 1139 5093 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3010 3439 2011 Size: 999 429 4804 Fragments arranged by size: 4804 999 429 BstYI r'GATC_y Cuts at: 1972 3468 3520 3562 3929 4314 4331 5783 5869 Size: 1496 52 42 367 385 17 1452 86 Cuts at: 5869 5881 6227 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1496 1452 385 367 346 86 52 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2400 2400 Size: 6232 BtgI C'CryG_G Cuts at: 513 1065 2010 2021 2328 2568 2740 3160 513 Size: 552 945 11 307 240 172 420 3585 Fragments arranged by size: 3585 945 552 420 307 240 172 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 2844 3050 3234 3748 4542 4562 2844 Size: 206 184 514 794 20 4514 Fragments arranged by size: 4514 794 514 206 184 20 ClaI AT'CG_AT Cuts at: 1554 2437 1554 Size: 883 5349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2245 2331 Size: 54 1425 50 275 18 225 190 86 Cuts at: 2331 2370 2502 2556 2637 2963 3000 3345 3679 Size: 39 132 54 81 326 37 345 334 Cuts at: 3679 4568 6197 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 345 334 326 275 225 190 132 86 81 54 54 50 43 39 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2245 3679 1537 Size: 275 433 1434 4090 Fragments arranged by size: 4090 1434 433 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2820 3134 4162 1353 Size: 1467 314 1028 3423 Fragments arranged by size: 3423 1467 1028 314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2058 4819 5193 5339 387 Size: 1064 138 469 2761 374 146 1280 Fragments arranged by size: 2761 1280 1064 469 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 3244 3371 4295 4892 5679 5947 Size: 393 704 908 127 924 597 787 268 Cuts at: 5947 1239 Size: 1524 Fragments arranged by size: 1524 924 908 787 704 597 393 268 127 EcoNI CCTnn'n_nnAGG Cuts at: 2318 3305 2318 Size: 987 5245 EcoO109I rG'GnC_Cy Cuts at: 2113 2305 2396 2406 2470 2471 2533 2787 2809 Size: 192 91 10 64 1 62 254 22 Cuts at: 2809 3045 3046 3310 3454 2113 Size: 236 1 264 144 4891 Fragments arranged by size: 4891 264 254 236 192 144 91 64 62 22 10 1 1 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3217 3217 Size: 6232 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2653 5379 1678 Size: 975 2726 2531 Fragments arranged by size: 2726 2531 975 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3460 3492 4873 4905 4947 Size: 32 135 32 1888 32 1381 32 42 Cuts at: 4947 4979 1373 Size: 32 2626 Fragments arranged by size: 2626 1888 1381 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3822 669 Size: 988 281 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5346 5530 1305 Size: 4041 184 2007 Fragments arranged by size: 4041 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2558 2965 3002 10 Size: 54 1425 343 726 407 37 3240 Fragments arranged by size: 3240 1425 726 407 343 54 37 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2366 2873 4190 4549 4708 518 Size: 648 843 357 507 1317 359 159 2042 Fragments arranged by size: 2042 1317 843 648 507 359 357 159 MspA1I CmG'CkG Cuts at: 1606 2023 2164 2742 2780 3289 3391 3416 3648 Size: 417 141 578 38 509 102 25 232 Cuts at: 3648 4325 5473 5718 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 578 509 417 245 232 141 102 38 25 NaeI GCC'GGC Cuts at: 2249 2374 2502 2249 Size: 125 128 5979 Fragments arranged by size: 5979 128 125 NcoI C'CATG_G Cuts at: 513 1065 2010 513 Size: 552 945 4735 Fragments arranged by size: 4735 945 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NgoMIV G'CCGG_C Cuts at: 2247 2372 2500 2247 Size: 125 128 5979 Fragments arranged by size: 5979 128 125 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2272 3590 5135 1132 Size: 97 1043 1318 1545 2229 Fragments arranged by size: 2229 1545 1318 1043 97 PasI CC'CwG_GG Cuts at: 1348 3211 1348 Size: 1863 4369 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 2536 2998 1108 Size: 1428 462 4342 Fragments arranged by size: 4342 1428 462 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2787 2809 3310 3454 2113 Size: 674 22 501 144 4891 Fragments arranged by size: 4891 674 501 144 22 PshAI GACnn'nnGTC Cuts at: 1613 2834 1613 Size: 1221 5011 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 2470 3045 2470 Size: 575 5657 PstI C_TGCA'G Cuts at: 830 2851 3241 3468 3584 830 Size: 2021 390 227 116 3478 Fragments arranged by size: 3478 2021 390 227 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2164 2780 3391 3416 2164 Size: 616 611 25 4980 Fragments arranged by size: 4980 616 611 25 SacII CC_GC'GG Cuts at: 2024 2743 2024 Size: 719 5513 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SanDI GG'GwC_CC Cuts at: 2787 2787 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2847 3237 3464 3580 4715 5396 5587 Size: 219 1802 390 227 116 1135 681 191 Cuts at: 5587 826 Size: 1471 Fragments arranged by size: 1802 1471 1135 681 390 227 219 191 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 2300 3446 3559 1872 Size: 428 1146 113 4545 Fragments arranged by size: 4545 1146 428 113 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 4336 5237 5499 820 Size: 197 926 1452 941 901 262 1553 Fragments arranged by size: 1553 1452 941 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2010 2386 2952 3066 3306 3671 513 Size: 552 945 376 566 114 240 365 3074 Fragments arranged by size: 3074 945 566 552 376 365 240 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2708 3224 4259 4276 4429 4614 5059 Size: 1255 1128 516 1035 17 153 185 445 Cuts at: 5059 325 Size: 1498 Fragments arranged by size: 1498 1255 1128 1035 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 3189 4478 Size: 274 80 33 51 1443 696 516 1289 Cuts at: 4478 6190 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1289 696 516 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 3155 1316 Size: 1839 4393 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 2551 2566 2809 3064 2152 Size: 399 15 243 255 5320 Fragments arranged by size: 5320 399 255 243 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 2298 3444 3557 1870 Size: 428 1146 113 4545 Fragments arranged by size: 4545 1146 428 113 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI