Restriction map for
pFN21AE0337
(Linear) (Six-base) MAPSORT of: KIEE0337.seq Check: 3465 from: 1 to: 1527
KIEE0337.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 1527
Size: 1366 161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 734 1527
Size: 734 793
AlwNI CAG_nnn'CTG
Cuts at: 0 480 531 1240 1527
Size: 480 51 709 287
Fragments arranged by size:
709 480 287 51
ApaI G_GGCC'C
Cuts at: 0 469 1044 1527
Size: 469 575 483
Fragments arranged by size:
575 483 469
ApaLI G'TGCA_C
Cuts at: 0 210 1527
Size: 210 1317
AvaI C'yCGr_G
Cuts at: 0 293 344 875 1439 1527
Size: 293 51 531 564 88
Fragments arranged by size:
564 531 293 88 51
BanI G'GyrC_C
Cuts at: 0 12 125 282 377 1527
Size: 12 113 157 95 1150
Fragments arranged by size:
1150 157 113 95 12
BanII G_rGCy'C
Cuts at: 0 469 1044 1111 1213 1280 1527
Size: 469 575 67 102 67 247
Fragments arranged by size:
575 469 247 102 67 67
BbvCI CC'TCA_GC
Cuts at: 0 1078 1527
Size: 1078 449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 866 900 1527
Size: 866 34 627
Fragments arranged by size:
866 627 34
BglI GCCn_nnn'nGGC
Cuts at: 0 582 1527
Size: 582 945
BglII A'GATC_T
Cuts at: 0 1463 1527
Size: 1463 64
Bme1580I G_kGCm'C
Cuts at: 0 15 128 214 287 469 1044 1099 1527
Size: 15 113 86 73 182 575 55 428
Fragments arranged by size:
575 428 182 113 86 73 55 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 1392 1527
Size: 1392 135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 370 1527
Size: 370 1157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1239 1527
Size: 331 908 288
Fragments arranged by size:
908 331 288
Bpu10I CC'TnA_GC
Cuts at: 0 1078 1527
Size: 1078 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1527
Size: 1375 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 853 898 1527
Size: 853 45 629
Fragments arranged by size:
853 629 45
BsaAI yAC'GTr
Cuts at: 0 195 1527
Size: 195 1332
BsaHI Gr'CG_yC
Cuts at: 0 275 638 1527
Size: 275 363 889
Fragments arranged by size:
889 363 275
BsaWI w'CCGG_w
Cuts at: 0 889 1527
Size: 889 638
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 328 1527
Size: 16 312 1199
Fragments arranged by size:
1199 312 16
BseYI C'CCAG_C
Cuts at: 0 130 286 641 1036 1092 1329 1426 1494
Size: 130 156 355 395 56 237 97 68
Cuts at: 1494 1527
Size: 33
Fragments arranged by size:
395 355 237 156 130 97 68 56
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1467 1527
Size: 1467 60
BsiEI CG_ry'CG
Cuts at: 0 243 1527
Size: 243 1284
BsiHKAI G_wGCw'C
Cuts at: 0 214 1527
Size: 214 1313
BsmBI CGTCTCn'nnnn_
Cuts at: 0 817 1527
Size: 817 710
Bsp1286I G_dGCh'C
Cuts at: 0 15 128 214 287 469 1044 1099 1111
Size: 15 113 86 73 182 575 55 12
Cuts at: 1111 1213 1280 1527
Size: 102 67 247
Fragments arranged by size:
575 247 182 113 102 86 73 67
55 15 12
BspHI T'CATG_A
Cuts at: 0 1272 1527
Size: 1272 255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 493 1019 1334 1527
Size: 493 526 315 193
Fragments arranged by size:
526 493 315 193
BsrDI GCAATG_nn'
Cuts at: 0 1506 1527
Size: 1506 21
BsrFI r'CCGG_y
Cuts at: 0 238 242 367 495 1527
Size: 238 4 125 128 1032
Fragments arranged by size:
1032 238 128 125 4
BssSI C'ACGA_G
Cuts at: 0 988 1527
Size: 988 539
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1240 1527
Size: 1240 287
BstEII G'GTnAC_C
Cuts at: 0 318 1527
Size: 318 1209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1005 1434 1527
Size: 1005 429 93
Fragments arranged by size:
1005 429 93
BstYI r'GATC_y
Cuts at: 0 1463 1515 1527
Size: 1463 52 12
Fragments arranged by size:
1463 52 12
Bsu36I CC'TnA_GG
Cuts at: 0 395 1527
Size: 395 1132
BtgI C'CryG_G
Cuts at: 0 5 16 323 563 735 1155 1527
Size: 5 11 307 240 172 420 372
Fragments arranged by size:
420 372 307 240 172 11 5
BtsI GCAGTG_nn'
Cuts at: 0 839 1045 1229 1527
Size: 839 206 184 298
Fragments arranged by size:
839 298 206 184
ClaI AT'CG_AT
Cuts at: 0 432 1527
Size: 432 1095
EaeI y'GGCC_r
Cuts at: 0 50 240 326 365 497 551 632 958
Size: 50 190 86 39 132 54 81 326
Cuts at: 958 995 1340 1527
Size: 37 345 187
Fragments arranged by size:
345 326 190 187 132 86 81 54
50 39 37
EagI C'GGCC_G
Cuts at: 0 240 1527
Size: 240 1287
EarI CTCTTCn'nnn_
Cuts at: 0 815 1129 1527
Size: 815 314 398
Fragments arranged by size:
815 398 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 53 1527
Size: 53 1474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1239 1366 1527
Size: 331 908 127 161
Fragments arranged by size:
908 331 161 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1300 1527
Size: 313 987 227
Fragments arranged by size:
987 313 227
EcoO109I rG'GnC_Cy
Cuts at: 0 108 300 391 401 465 466 528 782
Size: 108 192 91 10 64 1 62 254
Cuts at: 782 804 1040 1041 1305 1449 1527
Size: 22 236 1 264 144 78
Fragments arranged by size:
264 254 236 192 144 108 91 78
64 62 22 10 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1212 1527
Size: 1212 315
HaeII r_GCGC'y
Cuts at: 0 648 1527
Size: 648 879
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1455 1487 1527
Size: 1455 32 40
Fragments arranged by size:
1455 40 32
MscI TGG'CCA
Cuts at: 0 553 960 997 1527
Size: 553 407 37 530
Fragments arranged by size:
553 530 407 37
MslI CAynn'nnrTG
Cuts at: 0 361 868 1527
Size: 361 507 659
Fragments arranged by size:
659 507 361
MspA1I CmG'CkG
Cuts at: 0 18 159 737 775 1284 1386 1411 1527
Size: 18 141 578 38 509 102 25 116
Fragments arranged by size:
578 509 141 116 102 38 25 18
NaeI GCC'GGC
Cuts at: 0 244 369 497 1527
Size: 244 125 128 1030
Fragments arranged by size:
1030 244 128 125
NcoI C'CATG_G
Cuts at: 0 5 1527
Size: 5 1522
NgoMIV G'CCGG_C
Cuts at: 0 242 367 495 1527
Size: 242 125 128 1032
Fragments arranged by size:
1032 242 128 125
NspI r_CATG'y
Cuts at: 0 267 1527
Size: 267 1260
PasI CC'CwG_GG
Cuts at: 0 1206 1527
Size: 1206 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 993 1527
Size: 531 462 534
Fragments arranged by size:
534 531 462
PpuMI rG'GwC_Cy
Cuts at: 0 108 782 804 1305 1449 1527
Size: 108 674 22 501 144 78
Fragments arranged by size:
674 501 144 108 78 22
PshAI GACnn'nnGTC
Cuts at: 0 829 1527
Size: 829 698
PspOMI G'GGCC_C
Cuts at: 0 465 1040 1527
Size: 465 575 487
Fragments arranged by size:
575 487 465
PstI C_TGCA'G
Cuts at: 0 846 1236 1463 1527
Size: 846 390 227 64
Fragments arranged by size:
846 390 227 64
PvuII CAG'CTG
Cuts at: 0 159 775 1386 1411 1527
Size: 159 616 611 25 116
Fragments arranged by size:
616 611 159 116 25
SacII CC_GC'GG
Cuts at: 0 19 738 1527
Size: 19 719 789
Fragments arranged by size:
789 719 19
SanDI GG'GwC_CC
Cuts at: 0 782 1527
Size: 782 745
SfcI C'TryA_G
Cuts at: 0 842 1232 1459 1527
Size: 842 390 227 68
Fragments arranged by size:
842 390 227 68
SmaI CCC'GGG
Cuts at: 0 295 1441 1527
Size: 295 1146 86
Fragments arranged by size:
1146 295 86
SmlI C'TyrA_G
Cuts at: 0 1390 1527
Size: 1390 137
StyI C'CwwG_G
Cuts at: 0 5 381 947 1061 1301 1527
Size: 5 376 566 114 240 226
Fragments arranged by size:
566 376 240 226 114 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 703 1219 1527
Size: 703 516 308
Fragments arranged by size:
703 516 308
TatI w'GTAC_w
Cuts at: 0 668 1184 1527
Size: 668 516 343
Fragments arranged by size:
668 516 343
Tth111I GACn'n_nGTC
Cuts at: 0 1150 1527
Size: 1150 377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 546 561 804 1059 1527
Size: 147 399 15 243 255 468
Fragments arranged by size:
468 399 255 243 147 15
XmaI C'CCGG_G
Cuts at: 0 293 1439 1527
Size: 293 1146 88
Fragments arranged by size:
1146 293 88
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI AvaI BanI BanII
BbvCI BcgI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PpuMI PshAI PspOMI
PstI PvuII SacII SanDI SfcI SmaI SmlI StyI
TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI
BlpI BmgBI BmtI BsaBI BsaXI BsiWI BsmI BspEI
BsrBI BsrGI BssHII BstBI BstZ17I BtgZI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XhoI XmnI ZraI