Restriction map for
pFN21AA0006
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccII AclI AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BbeI BbrPI
BcgI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KspI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1406 GGTACC G^GTACC
AccIII 78 TCCGGA T^CCGGA
AflII 801 CTTAAG C^TTAAG
AlwNI 1288 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2071 CCTGAGG CC^T[ACGT]AGG
Asp718I 1406 GGTACC G^GTACC
AspI 2167 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1727 CCCGAG C^[CT]CG[GA]G
BamHI 174 GGATCC G^GATCC
BanI 1406 GGTACC G^G[CT][GA]CC
BanII 1086 GAGCCC G[GA]GC[CT]^C
BbsI 1899 GAAGAC GAAGAC(2/6)
BbvCI 1116 CCTCAGC CCTCAGC(-5/-2)
BfrI 801 CTTAAG C^TTAAG
BglII 1257 AGATCT A^GATCT
BpuAI 1899 GAAGAC GAAGAC(2/6)
BsaBI 1186 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 663 GAGACC GGTCTC(1/5)
BseAI 78 TCCGGA T^CCGGA
BsmI 1228 GCATTC GAATGC(1/-1)
BsoBI 1727 CCCGAG C^[CT]CG[GA]G
BspEI 78 TCCGGA T^CCGGA
BsrBI 1402 GAGCGG CCGCTC(-3/-3)
BssSI 569 CACGAG CACGAG(-5/-1)
Bsu36I 2071 CCTGAGG CC^T[ACGT]AGG
CfoI 2006 GCGC GCG^C
EaeI 2112 CGGCCA [CT]^GGCC[GA]
Eco81I 2071 CCTGAGG CC^T[ACGT]AGG
HhaI 2006 GCGC GCG^C
HinP1I 2006 GCGC G^CGC
HincII 316 GTCAAC GT[CT]^[GA]AC
HindII 316 GTCAAC GT[CT]^[GA]AC
HphI 1506 GGTGA GGTGA(8/7)
KpnI 1406 GGTACC GGTAC^C
MamI 1186 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 78 TCCGGA T^CCGGA
MslI 1042 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2025 CCATGG C^CATGG
NdeI 1177 CATATG CA^TATG
NspI 1384 GCATGT [GA]CATG^[CT]
PflFI 2167 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 900 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 811 CTGCAG CTGCA^G
SfcI 811 CTGCAG C^T[GA][CT]AG
SspI 249 AATATT AAT^ATT
Tth111I 2167 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 900 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 956 GTCTAC GT^[AC][GT]AC
1778 GTCTAC
AciI 1404 GCGG CCGC(-3/-1)
2259 GCGG
AcsI 1882 AAATTT [GA]^AATT[CT]
2010 AAATTT
ApoI 1882 AAATTT [GA]^AATT[CT]
2010 AAATTT
Asp700I 1300 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2042 GAAAAGATTC
AspHI 1030 GTGCTC G[AT]GC[AT]^C
1694 GTGCTC
BciVI 1185 GGATAC GTATCC(6/5)
1094 GTATCC
BclI 131 TGATCA T^GATCA
1637 TGATCA
BpmI 1138 CTGGAG CTGGAG(16/14)
1279 CTGGAG
BsaWI 78 TCCGGA [AT]^CCGG[AT]
1717 ACCGGA
BsiHKAI 1030 GTGCTC G[AT]GC[AT]^C
1694 GTGCTC
BspMI 1647 ACCTGC ACCTGC(4/8)
1785 ACCTGC
BsrDI 1248 GCAATG GCAATG(2/0)
2208 GCAATG
BstYI 174 GGATCC [GA]^GATC[CT]
1257 AGATCT
DraI 90 TTTAAA TTT^AAA
979 TTTAAA
DsaI 1762 CCGTGG C^C[GA][CT]GG
2025 CCATGG
FbaI 131 TGATCA T^GATCA
1637 TGATCA
HaeIII 2023 GGCC GG^CC
2113 GGCC
HapII 79 CCGG C^CGG
1718 CCGG
HpaII 79 CCGG C^CGG
1718 CCGG
MaeII 360 ACGT A^CGT
563 ACGT
MflI 174 GGATCC [GA]^GATC[CT]
1257 AGATCT
MspI 79 CCGG C^CGG
1718 CCGG
SmlI 801 CTTAAG C^T[CT][GA]AG
2031 CTCAAG
XhoII 174 GGATCC [GA]^GATC[CT]
1257 AGATCT
XmnI 1300 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2042 GAAAAGATTC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BfaI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I