Restriction map for pFN21AA0006
 (Circular) (Six-base) MAPSORT of: pFN21AA0006.seq  Check: 4821  from: 1  to: 7033



pFN21AA0006  7033 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4843     278 
   Size:         53      83     186     936    3307    2468

  Fragments arranged by size: 

                3307    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2962    3784    4376    1937 
   Size:       1025     822     592    4594

  Fragments arranged by size: 

                4594    1025     822     592
 
Acc65I G'GTAC_C

Cuts at:   3411    4354    3411 
   Size:        943    6090
 
AclI AA'CG_TT

Cuts at:   1563    5160    5533    1563 
   Size:       3597     373    3063

  Fragments arranged by size: 

                3597    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2211    2768    3006    4208    5096    6480    6748    1632 
   Size:        579     557     238    1202     888    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1202     888     579     557     268     238
            
 
AflII C'TTAA_G

Cuts at:    820    1017    2806     820 
   Size:        197    1789    5047

  Fragments arranged by size: 

                5047    1789     197
 
AflIII A'CryG_T

Cuts at:   1203    5932    6909    1203 
   Size:       4729     977    1327

  Fragments arranged by size: 

                4729    1327     977
 
AgeI A'CCGG_T

Cuts at:   5864    5864 
   Size:       7033
 
AhdI GACnn_n'nnGTC

Cuts at:   5762    5762 
   Size:       7033
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7033
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7033
 
AlwNI CAG_nnn'CTG

Cuts at:   3298    4776    6348    3298 
   Size:       1478    1572    3983

  Fragments arranged by size: 

                3983    1572    1478
 
ApaLI G'TGCA_C

Cuts at:   5090    6246    5090 
   Size:       1156    5877
 
ApoI r'AATT_y

Cuts at:   1493    3887    4015    4342    4568    4720    6829    1493 
   Size:       2394     128     327     226     152    2109    1697

  Fragments arranged by size: 

                2394    2109    1697     327     226     152     128
 
AseI AT'TA_AT

Cuts at:    160    1032    5587     160 
   Size:        872    4555    1606

  Fragments arranged by size: 

                4555    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7033
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3732    4358    1870 
   Size:         73    1789     626    4545

  Fragments arranged by size: 

                4545    1789     626      73
 
BamHI G'GATC_C

Cuts at:   2179    4363    4730    2179 
   Size:       2184     367    4482

  Fragments arranged by size: 

                4482    2184     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3411    4354    5809     618 
   Size:        325     217     627    1624     943    1455    1842

  Fragments arranged by size: 

                1842    1624    1455     943     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3095    4352    1391 
   Size:       1704    1257    4072

  Fragments arranged by size: 

                4072    1704    1257
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3911     928 
   Size:        971    2012    4050

  Fragments arranged by size: 

                4050    2012     971
 
BbvCI CC'TCA_GC

Cuts at:   3122    3122 
   Size:       7033
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5224    5258    1903 
   Size:         34    3287      34    3678

  Fragments arranged by size: 

                3678    3287      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3110    3184    4925    6135    3110 
   Size:         74    1741    1210    4008

  Fragments arranged by size: 

                4008    1741    1210      74
 
BclI T'GATC_A

Cuts at:   1548    2136    3642    1548 
   Size:        588    1506    4939

  Fragments arranged by size: 

                4939    1506     588
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5644     136 
   Size:        107     122      71     631     605    3972    1525

  Fragments arranged by size: 

                3972    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   3262    7028    3262 
   Size:       3766    3267
 
BlpI GC'TnA_GC

Cuts at:   4450    4450 
   Size:       7033
 
Bme1580I G_kGCm'C

Cuts at:   1790    5094    6250    1790 
   Size:       3304    1156    2573

  Fragments arranged by size: 

                3304    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7033
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2628    2870    4764 
   Size:        416      53      83     399      24    1211     242    1894

Cuts at:   4764    5722     442 
   Size:        958    1753

  Fragments arranged by size: 

                1894    1753    1211     958     416     399     242      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7033
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3164    3305    5693    1239 
   Size:       1925     141    2388    2579

  Fragments arranged by size: 

                2579    2388    1925     141
 
Bpu10I CC'TnA_GC

Cuts at:   3122    3122 
   Size:       7033
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4021    5158    6023    6321    4021 
   Size:       1137     865     298    4733

  Fragments arranged by size: 

                4733    1137     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2662    5696     882 
   Size:        617    1163    3034    2219

  Fragments arranged by size: 

                3034    2219    1163     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4824     493 
   Size:        711    3620    2702

  Fragments arranged by size: 

                3620    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3195    7027    3195 
   Size:       3832    3201
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4840    5222    5857     275 
   Size:         53      83     186     936    3307     382     635    1451

  Fragments arranged by size: 

                3307    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2083    3722    5466    5864    6138    6285    6696    2083 
   Size:       1639    1744     398     274     147     411    2420

  Fragments arranged by size: 

                2420    1744    1639     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3763    3793    1178 
   Size:         30     165      30    2360      30    4418

  Fragments arranged by size: 

                4418    2360     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2102    3419    3741    3941    1186 
   Size:        916    1317     322     200    4278

  Fragments arranged by size: 

                4278    1317     916     322     200
 
BseYI C'CCAG_C

Cuts at:   6236    6732    6236 
   Size:        496    6537
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3398    3529    3777    3988    1163 
   Size:        573    1662     131     248     211    4208

  Fragments arranged by size: 

                4208    1662     573     248     211     131
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4483    5244    6272    1540 
   Size:        275     186    2482     761    1028    2301

  Fragments arranged by size: 

                2482    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3039    3703    5094    5179    6250    3039 
   Size:        664    1391      85    1071    3822

  Fragments arranged by size: 

                3822    1391    1071     664      85
 
BsmI GAATG_Cn'

Cuts at:   3233    4544    4637    3233 
   Size:       1311      93    5629

  Fragments arranged by size: 

                5629    1311      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3039    3095    3703    4352    5094    5179    6250 
   Size:        399    1249      56     608     649     742      85    1071

Cuts at:   6250    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1249    1071     742     649     608     399      85
                  56
 
BspEI T'CCGG_A

Cuts at:   2083    6696    2083 
   Size:       4613    2420
 
BspHI T'CATG_A

Cuts at:   4922    6652    4922 
   Size:       1730    5303
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3661    3799    4388     844 
   Size:       2817     138     589    3489

  Fragments arranged by size: 

                3489    2817     589     138
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3409    4920    1394 
   Size:        429    1586    1511    3507

  Fragments arranged by size: 

                3507    1586    1511     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3260    4220    5528    5702      65 
   Size:       1183    2012     960    1308     174    1396

  Fragments arranged by size: 

                2012    1396    1308    1183     960     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5677    5864    1700 
   Size:        110    3867     187    2869

  Fragments arranged by size: 

                3867    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7033
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7033
 
BssSI C'ACGA_G

Cuts at:   2574    5093    6105    2574 
   Size:       2519    1012    3502

  Fragments arranged by size: 

                3502    2519    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7033
 
BstBI TT'CG_AA

Cuts at:   5837    5837 
   Size:       7033
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7033
 
BstYI r'GATC_y

Cuts at:   1972    2179    3262    4363    4730    5115    5132    6584    6670 
   Size:        207    1083    1101     367     385      17    1452      86

Cuts at:   6670    6682    7028    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1101    1083     385     367     346     207
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   4077    4077 
   Size:       7033
 
BtgI C'CryG_G

Cuts at:    513    1065    3767    4030     513 
   Size:        552    2702     263    3516

  Fragments arranged by size: 

                3516    2702     552     263
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6294

  Fragments arranged by size: 

                6294     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4549    5343    5363    4549 
   Size:        794      20    6219

  Fragments arranged by size: 

                6219     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7033
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2442    2477     529 
   Size:         35    1878      35    5085

  Fragments arranged by size: 

                5085    1878      35      35
 
DraI TTT'AAA

Cuts at:   2097    2986    4337    2097 
   Size:        889    1351    4793

  Fragments arranged by size: 

                4793    1351     889
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6040     809 
   Size:        507    4724    1802

  Fragments arranged by size: 

                4724    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4117    4480    5369 
   Size:         54    1425      50     275      18    2287     363     889

Cuts at:   5369    6998       8 
   Size:       1629      43

  Fragments arranged by size: 

                2287    1629    1425     889     363     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4480    1537 
   Size:        275    2668    4090

  Fragments arranged by size: 

                4090    2668     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2377    2796    3266    3960    4077    4963    1353 
   Size:       1024     419     470     694     117     886    3423

  Fragments arranged by size: 

                3423    1024     886     694     470     419     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5620    5994    6140     387 
   Size:       1064     138    4031     374     146    1280

  Fragments arranged by size: 

                4031    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2211    2768    3006    3164    3305    4208    5096 
   Size:        393     579     557     238     158     141     903     888

Cuts at:   5096    5693    6480    6748    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     903     888     787     597     579     557     393
                 268     238     158     141
 
EcoO109I rG'GnC_Cy

Cuts at:   3650    3737    4246    3650 
   Size:         87     509    6437

  Fragments arranged by size: 

                6437     509      87
 
EcoRI G'AATT_C

Cuts at:   4342    4720    4342 
   Size:        378    6655
 
FspI TGC'GCA

Cuts at:   5539    5539 
   Size:       7033
 
HaeII r_GCGC'y

Cuts at:   1678    6180    1678 
   Size:       4502    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2330    2362    2525    2557    2837 
   Size:         32     135      32     758      32     163      32     280

Cuts at:   2837    2869    3824    3856    5674    5706    5748    5780    1373 
   Size:         32     955      32    1818      32      42      32    2626

  Fragments arranged by size: 

                2626    1818     955     758     280     163     135      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2323    4377    4623     669 
   Size:        988     281     385    2054     246    3079

  Fragments arranged by size: 

                3079    2054     988     385     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       7033
 
HpaI GTT'AAC

Cuts at:   4623    4623 
   Size:       7033
 
KpnI G_GTAC'C

Cuts at:   3415    4358    3415 
   Size:        943    6090
 
MfeI C'AATT_G

Cuts at:   4632    4632 
   Size:       7033
 
MluI A'CGCG_T

Cuts at:   6909    6909 
   Size:       7033
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3039    3603    6147    6331    1305 
   Size:       1734     564    2544     184    2007

  Fragments arranged by size: 

                2544    2007    1734     564     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5211

  Fragments arranged by size: 

                5211    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3051    4991    5350    5509     518 
   Size:        648    1885    1940     359     159    2042

  Fragments arranged by size: 

                2042    1940    1885     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3026    3283    3631    3956    4449    5126    6274    6519 
   Size:       1420     257     348     325     493     677    1148     245

Cuts at:   6519    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1420    1148     677     493     348     325     257
                 245
 
NcoI C'CATG_G

Cuts at:    513    1065    4030     513 
   Size:        552    2965    3516

  Fragments arranged by size: 

                3516    2965     552
 
NdeI CA'TA_TG

Cuts at:    387    3183     387 
   Size:       2796    4237
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7033
 
NotI GC'GGCC_GC

Cuts at:   4480    4480 
   Size:       7033
 
NspI r_CATG'y

Cuts at:   1132    1229    3393    4391    5936    1132 
   Size:         97    2164     998    1545    2229

  Fragments arranged by size: 

                2229    2164    1545     998      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7033
 
PciI A'CATG_T

Cuts at:   5932    5932 
   Size:       7033
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2911    1108 
   Size:       1803    5230
 
PfoI T'CCnGG_A

Cuts at:   2751    3612    2751 
   Size:        861    6172
 
PmeI GTTT'AAAC

Cuts at:   4337    4337 
   Size:       7033
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5102    5134    6615    6647    5102 
   Size:         32    1481      32    5488

  Fragments arranged by size: 

                5488    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3650    3737    4246    3650 
   Size:         87     509    6437

  Fragments arranged by size: 

                6437     509      87
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7033
 
PsiI TTA'TAA

Cuts at:   3761    3828    4603    3761 
   Size:         67     775    6191

  Fragments arranged by size: 

                6191     775      67
 
PstI C_TGCA'G

Cuts at:    830    2820    4385     830 
   Size:       1990    1565    3478

  Fragments arranged by size: 

                3478    1990    1565
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7033
 
PvuII CAG'CTG

Cuts at:   3026    3283    3631    3956    3026 
   Size:        257     348     325    6103

  Fragments arranged by size: 

                6103     348     325     257
 
SalI G'TCGA_C

Cuts at:   1936    4375    1936 
   Size:       2439    4594
 
SbfI CC_TGCA'GG

Cuts at:   4385    4385 
   Size:       7033
 
ScaI AGT'ACT

Cuts at:   2308    2839    4042    5281    6993    2308 
   Size:        531    1203    1239    1712    2348

  Fragments arranged by size: 

                2348    1712    1239    1203     531
 
SfcI C'TryA_G

Cuts at:    826    1045    2816    4381    5516    6197    6388     826 
   Size:        219    1771    1565    1135     681     191    1471

  Fragments arranged by size: 

                1771    1565    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7033
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7033
 
SmaI CCC'GGG

Cuts at:   1872    4360    1872 
   Size:       2488    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2806    4036    5137    6038    6300     820 
   Size:        197     926     863    1230    1101     901     262    1553

  Fragments arranged by size: 

                1553    1230    1101     926     901     863     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4824     493 
   Size:       4331    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7033
 
SphI G_CATG'C

Cuts at:   1132    4391    1132 
   Size:       3259    3774
 
SspI AAT'ATT

Cuts at:      5      52    2256    4957       5 
   Size:         47    2204    2701    2081

  Fragments arranged by size: 

                2701    2204    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       7033
 
StyI C'CwwG_G

Cuts at:    513    1065    2295    2910    3741    4030    4472     513 
   Size:        552    1230     615     831     289     442    3074

  Fragments arranged by size: 

                3074    1230     831     615     552     442     289
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    5060    5077    5230    5415    5860     325 
   Size:       1255    3480      17     153     185     445    1498

  Fragments arranged by size: 

                3480    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2306    2837    3950 
   Size:        274      80      33      51    1443     329     531    1113

Cuts at:   3950    4040    5279    6991      96 
   Size:         90    1239    1712     138

  Fragments arranged by size: 

                1712    1443    1239    1113     531     329     274     138
                  90      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3495    3527    6993    7025    3495 
   Size:         32    3466      32    3503

  Fragments arranged by size: 

                3503    3466      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    4175    1316 
   Size:       2859    4174
 
XbaI T'CTAG_A

Cuts at:   4369    4369 
   Size:       7033
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7033
 
XmaI C'CCGG_G

Cuts at:   1870    4358    1870 
   Size:       2488    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3309    4051    5162    3309 
   Size:        742    1111    5180

  Fragments arranged by size: 

                5180    1111     742
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4841     276 
   Size:         53      83     186     936    3307    2468

  Fragments arranged by size: 

                3307    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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