(Circular) (Six-base) MAPSORT of: pFN21AA0006.seq Check: 4821 from: 1 to: 7033 pFN21AA0006 7033 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2962 3784 4376 1937 Size: 1025 822 592 4594 Fragments arranged by size: 4594 1025 822 592 Acc65I G'GTAC_C Cuts at: 3411 4354 3411 Size: 943 6090 AclI AA'CG_TT Cuts at: 1563 5160 5533 1563 Size: 3597 373 3063 Fragments arranged by size: 3597 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 2768 3006 4208 5096 6480 6748 1632 Size: 579 557 238 1202 888 1384 268 1917 Fragments arranged by size: 1917 1384 1202 888 579 557 268 238 AflII C'TTAA_G Cuts at: 820 1017 2806 820 Size: 197 1789 5047 Fragments arranged by size: 5047 1789 197 AflIII A'CryG_T Cuts at: 1203 5932 6909 1203 Size: 4729 977 1327 Fragments arranged by size: 4729 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AlwNI CAG_nnn'CTG Cuts at: 3298 4776 6348 3298 Size: 1478 1572 3983 Fragments arranged by size: 3983 1572 1478 ApaLI G'TGCA_C Cuts at: 5090 6246 5090 Size: 1156 5877 ApoI r'AATT_y Cuts at: 1493 3887 4015 4342 4568 4720 6829 1493 Size: 2394 128 327 226 152 2109 1697 Fragments arranged by size: 2394 2109 1697 327 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 3732 4358 1870 Size: 73 1789 626 4545 Fragments arranged by size: 4545 1789 626 73 BamHI G'GATC_C Cuts at: 2179 4363 4730 2179 Size: 2184 367 4482 Fragments arranged by size: 4482 2184 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3411 4354 5809 618 Size: 325 217 627 1624 943 1455 1842 Fragments arranged by size: 1842 1624 1455 943 627 325 217 BanII G_rGCy'C Cuts at: 1391 3095 4352 1391 Size: 1704 1257 4072 Fragments arranged by size: 4072 1704 1257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3911 928 Size: 971 2012 4050 Fragments arranged by size: 4050 2012 971 BbvCI CC'TCA_GC Cuts at: 3122 3122 Size: 7033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3110 3184 4925 6135 3110 Size: 74 1741 1210 4008 Fragments arranged by size: 4008 1741 1210 74 BclI T'GATC_A Cuts at: 1548 2136 3642 1548 Size: 588 1506 4939 Fragments arranged by size: 4939 1506 588 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5644 136 Size: 107 122 71 631 605 3972 1525 Fragments arranged by size: 3972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3262 7028 3262 Size: 3766 3267 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 5094 6250 1790 Size: 3304 1156 2573 Fragments arranged by size: 3304 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2628 2870 4764 Size: 416 53 83 399 24 1211 242 1894 Cuts at: 4764 5722 442 Size: 958 1753 Fragments arranged by size: 1894 1753 1211 958 416 399 242 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3164 3305 5693 1239 Size: 1925 141 2388 2579 Fragments arranged by size: 2579 2388 1925 141 Bpu10I CC'TnA_GC Cuts at: 3122 3122 Size: 7033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 5158 6023 6321 4021 Size: 1137 865 298 4733 Fragments arranged by size: 4733 1137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2662 5696 882 Size: 617 1163 3034 2219 Fragments arranged by size: 3034 2219 1163 617 BsaAI yAC'GTr Cuts at: 493 1204 4824 493 Size: 711 3620 2702 Fragments arranged by size: 3620 2702 711 BsaBI GATnn'nnATC Cuts at: 3195 7027 3195 Size: 3832 3201 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4840 5222 5857 275 Size: 53 83 186 936 3307 382 635 1451 Fragments arranged by size: 3307 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2083 3722 5466 5864 6138 6285 6696 2083 Size: 1639 1744 398 274 147 411 2420 Fragments arranged by size: 2420 1744 1639 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3763 3793 1178 Size: 30 165 30 2360 30 4418 Fragments arranged by size: 4418 2360 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 3419 3741 3941 1186 Size: 916 1317 322 200 4278 Fragments arranged by size: 4278 1317 916 322 200 BseYI C'CCAG_C Cuts at: 6236 6732 6236 Size: 496 6537 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3398 3529 3777 3988 1163 Size: 573 1662 131 248 211 4208 Fragments arranged by size: 4208 1662 573 248 211 131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4483 5244 6272 1540 Size: 275 186 2482 761 1028 2301 Fragments arranged by size: 2482 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3039 3703 5094 5179 6250 3039 Size: 664 1391 85 1071 3822 Fragments arranged by size: 3822 1391 1071 664 85 BsmI GAATG_Cn' Cuts at: 3233 4544 4637 3233 Size: 1311 93 5629 Fragments arranged by size: 5629 1311 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3039 3095 3703 4352 5094 5179 6250 Size: 399 1249 56 608 649 742 85 1071 Cuts at: 6250 1391 Size: 2174 Fragments arranged by size: 2174 1249 1071 742 649 608 399 85 56 BspEI T'CCGG_A Cuts at: 2083 6696 2083 Size: 4613 2420 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3661 3799 4388 844 Size: 2817 138 589 3489 Fragments arranged by size: 3489 2817 589 138 BsrBI CCG'CTC Cuts at: 1394 1823 3409 4920 1394 Size: 429 1586 1511 3507 Fragments arranged by size: 3507 1586 1511 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3260 4220 5528 5702 65 Size: 1183 2012 960 1308 174 1396 Fragments arranged by size: 2012 1396 1308 1183 960 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5677 5864 1700 Size: 110 3867 187 2869 Fragments arranged by size: 3867 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 2574 5093 6105 2574 Size: 2519 1012 3502 Fragments arranged by size: 3502 2519 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7033 BstBI TT'CG_AA Cuts at: 5837 5837 Size: 7033 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstYI r'GATC_y Cuts at: 1972 2179 3262 4363 4730 5115 5132 6584 6670 Size: 207 1083 1101 367 385 17 1452 86 Cuts at: 6670 6682 7028 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1101 1083 385 367 346 207 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4077 4077 Size: 7033 BtgI C'CryG_G Cuts at: 513 1065 3767 4030 513 Size: 552 2702 263 3516 Fragments arranged by size: 3516 2702 552 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 4549 5343 5363 4549 Size: 794 20 6219 Fragments arranged by size: 6219 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2442 2477 529 Size: 35 1878 35 5085 Fragments arranged by size: 5085 1878 35 35 DraI TTT'AAA Cuts at: 2097 2986 4337 2097 Size: 889 1351 4793 Fragments arranged by size: 4793 1351 889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4117 4480 5369 Size: 54 1425 50 275 18 2287 363 889 Cuts at: 5369 6998 8 Size: 1629 43 Fragments arranged by size: 2287 1629 1425 889 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4480 1537 Size: 275 2668 4090 Fragments arranged by size: 4090 2668 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2377 2796 3266 3960 4077 4963 1353 Size: 1024 419 470 694 117 886 3423 Fragments arranged by size: 3423 1024 886 694 470 419 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2768 3006 3164 3305 4208 5096 Size: 393 579 557 238 158 141 903 888 Cuts at: 5096 5693 6480 6748 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 903 888 787 597 579 557 393 268 238 158 141 EcoO109I rG'GnC_Cy Cuts at: 3650 3737 4246 3650 Size: 87 509 6437 Fragments arranged by size: 6437 509 87 EcoRI G'AATT_C Cuts at: 4342 4720 4342 Size: 378 6655 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 6180 1678 Size: 4502 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 2525 2557 2837 Size: 32 135 32 758 32 163 32 280 Cuts at: 2837 2869 3824 3856 5674 5706 5748 5780 1373 Size: 32 955 32 1818 32 42 32 2626 Fragments arranged by size: 2626 1818 955 758 280 163 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2323 4377 4623 669 Size: 988 281 385 2054 246 3079 Fragments arranged by size: 3079 2054 988 385 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7033 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KpnI G_GTAC'C Cuts at: 3415 4358 3415 Size: 943 6090 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3039 3603 6147 6331 1305 Size: 1734 564 2544 184 2007 Fragments arranged by size: 2544 2007 1734 564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5211 Fragments arranged by size: 5211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3051 4991 5350 5509 518 Size: 648 1885 1940 359 159 2042 Fragments arranged by size: 2042 1940 1885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3026 3283 3631 3956 4449 5126 6274 6519 Size: 1420 257 348 325 493 677 1148 245 Cuts at: 6519 1606 Size: 2120 Fragments arranged by size: 2120 1420 1148 677 493 348 325 257 245 NcoI C'CATG_G Cuts at: 513 1065 4030 513 Size: 552 2965 3516 Fragments arranged by size: 3516 2965 552 NdeI CA'TA_TG Cuts at: 387 3183 387 Size: 2796 4237 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 3393 4391 5936 1132 Size: 97 2164 998 1545 2229 Fragments arranged by size: 2229 2164 1545 998 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 2911 1108 Size: 1803 5230 PfoI T'CCnGG_A Cuts at: 2751 3612 2751 Size: 861 6172 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3650 3737 4246 3650 Size: 87 509 6437 Fragments arranged by size: 6437 509 87 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 3761 3828 4603 3761 Size: 67 775 6191 Fragments arranged by size: 6191 775 67 PstI C_TGCA'G Cuts at: 830 2820 4385 830 Size: 1990 1565 3478 Fragments arranged by size: 3478 1990 1565 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 3026 3283 3631 3956 3026 Size: 257 348 325 6103 Fragments arranged by size: 6103 348 325 257 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 2308 2839 4042 5281 6993 2308 Size: 531 1203 1239 1712 2348 Fragments arranged by size: 2348 1712 1239 1203 531 SfcI C'TryA_G Cuts at: 826 1045 2816 4381 5516 6197 6388 826 Size: 219 1771 1565 1135 681 191 1471 Fragments arranged by size: 1771 1565 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2806 4036 5137 6038 6300 820 Size: 197 926 863 1230 1101 901 262 1553 Fragments arranged by size: 1553 1230 1101 926 901 863 262 197 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SspI AAT'ATT Cuts at: 5 52 2256 4957 5 Size: 47 2204 2701 2081 Fragments arranged by size: 2701 2204 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2295 2910 3741 4030 4472 513 Size: 552 1230 615 831 289 442 3074 Fragments arranged by size: 3074 1230 831 615 552 442 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5060 5077 5230 5415 5860 325 Size: 1255 3480 17 153 185 445 1498 Fragments arranged by size: 3480 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2306 2837 3950 Size: 274 80 33 51 1443 329 531 1113 Cuts at: 3950 4040 5279 6991 96 Size: 90 1239 1712 138 Fragments arranged by size: 1712 1443 1239 1113 531 329 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3495 3527 6993 7025 3495 Size: 32 3466 32 3503 Fragments arranged by size: 3503 3466 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4175 1316 Size: 2859 4174 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 3309 4051 5162 3309 Size: 742 1111 5180 Fragments arranged by size: 5180 1111 742 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI