Restriction map for
pFN21AA0006
(Linear) (Six-base) MAPSORT of: KIAA0006.seq Check: 9487 from: 1 to: 2328
KIAA0006.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 957 1779 2328
Size: 957 822 549
Fragments arranged by size:
957 822 549
Acc65I G'GTAC_C
Cuts at: 0 1406 2328
Size: 1406 922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 763 1001 2203 2328
Size: 206 557 238 1202 125
Fragments arranged by size:
1202 557 238 206 125
AflII C'TTAA_G
Cuts at: 0 801 2328
Size: 801 1527
AlwNI CAG_nnn'CTG
Cuts at: 0 1293 2328
Size: 1293 1035
ApoI r'AATT_y
Cuts at: 0 1882 2010 2328
Size: 1882 128 318
Fragments arranged by size:
1882 318 128
AvaI C'yCGr_G
Cuts at: 0 1727 2328
Size: 1727 601
BamHI G'GATC_C
Cuts at: 0 174 2328
Size: 174 2154
BanI G'GyrC_C
Cuts at: 0 1406 2328
Size: 1406 922
BanII G_rGCy'C
Cuts at: 0 1090 2328
Size: 1090 1238
BbsI GAAGACnn'nnnn_
Cuts at: 0 1906 2328
Size: 1906 422
BbvCI CC'TCA_GC
Cuts at: 0 1117 2328
Size: 1117 1211
BciVI GTATCCnnnnn_n'
Cuts at: 0 1105 1179 2328
Size: 1105 74 1149
Fragments arranged by size:
1149 1105 74
BclI T'GATC_A
Cuts at: 0 131 1637 2328
Size: 131 1506 691
Fragments arranged by size:
1506 691 131
BglII A'GATC_T
Cuts at: 0 1257 2328
Size: 1257 1071
BmrI ACTGGGnnnn_n'
Cuts at: 0 623 865 2328
Size: 623 242 1463
Fragments arranged by size:
1463 623 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1300 2328
Size: 1159 141 1028
Fragments arranged by size:
1159 1028 141
Bpu10I CC'TnA_GC
Cuts at: 0 1117 2328
Size: 1117 1211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2016 2328
Size: 2016 312
BsaI GGTCTCn'nnnn_
Cuts at: 0 657 2328
Size: 657 1671
BsaBI GATnn'nnATC
Cuts at: 0 1190 2328
Size: 1190 1138
BsaWI w'CCGG_w
Cuts at: 0 78 1717 2328
Size: 78 1639 611
Fragments arranged by size:
1639 611 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1758 1788 2328
Size: 1758 30 540
Fragments arranged by size:
1758 540 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 1414 1736 1936 2328
Size: 97 1317 322 200 392
Fragments arranged by size:
1317 392 322 200 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1524 1772 1983 2328
Size: 1393 131 248 211 345
Fragments arranged by size:
1393 345 248 211 131
BsiHKAI G_wGCw'C
Cuts at: 0 1034 1698 2328
Size: 1034 664 630
Fragments arranged by size:
1034 664 630
BsmI GAATG_Cn'
Cuts at: 0 1228 2328
Size: 1228 1100
Bsp1286I G_dGCh'C
Cuts at: 0 1034 1090 1698 2328
Size: 1034 56 608 630
Fragments arranged by size:
1034 630 608 56
BspEI T'CCGG_A
Cuts at: 0 78 2328
Size: 78 2250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1656 1794 2328
Size: 1656 138 534
Fragments arranged by size:
1656 534 138
BsrBI CCG'CTC
Cuts at: 0 1404 2328
Size: 1404 924
BsrDI GCAATG_nn'
Cuts at: 0 1255 2215 2328
Size: 1255 960 113
Fragments arranged by size:
1255 960 113
BssSI C'ACGA_G
Cuts at: 0 569 2328
Size: 569 1759
BstYI r'GATC_y
Cuts at: 0 174 1257 2328
Size: 174 1083 1071
Fragments arranged by size:
1083 1071 174
Bsu36I CC'TnA_GG
Cuts at: 0 2072 2328
Size: 2072 256
BtgI C'CryG_G
Cuts at: 0 1762 2025 2328
Size: 1762 263 303
Fragments arranged by size:
1762 303 263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 437 472 2328
Size: 437 35 1856
Fragments arranged by size:
1856 437 35
DraI TTT'AAA
Cuts at: 0 92 981 2328
Size: 92 889 1347
Fragments arranged by size:
1347 889 92
EaeI y'GGCC_r
Cuts at: 0 2112 2328
Size: 2112 216
EarI CTCTTCn'nnn_
Cuts at: 0 372 791 1261 1955 2072 2328
Size: 372 419 470 694 117 256
Fragments arranged by size:
694 470 419 372 256 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 763 1001 1159 1300 2203 2328
Size: 206 557 238 158 141 903 125
Fragments arranged by size:
903 557 238 206 158 141 125
EcoO109I rG'GnC_Cy
Cuts at: 0 1645 1732 2241 2328
Size: 1645 87 509 87
Fragments arranged by size:
1645 509 87 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 520 552 832 864 1819 1851
Size: 325 32 163 32 280 32 955 32
Cuts at: 1851 2328
Size: 477
Fragments arranged by size:
955 477 325 280 163 32 32 32
32
HincII GTy'rAC
Cuts at: 0 318 2328
Size: 318 2010
KpnI G_GTAC'C
Cuts at: 0 1410 2328
Size: 1410 918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1598 2328
Size: 1034 564 730
Fragments arranged by size:
1034 730 564
MslI CAynn'nnrTG
Cuts at: 0 1046 2328
Size: 1046 1282
MspA1I CmG'CkG
Cuts at: 0 1021 1278 1626 1951 2328
Size: 1021 257 348 325 377
Fragments arranged by size:
1021 377 348 325 257
NcoI C'CATG_G
Cuts at: 0 2025 2328
Size: 2025 303
NdeI CA'TA_TG
Cuts at: 0 1178 2328
Size: 1178 1150
NspI r_CATG'y
Cuts at: 0 1388 2328
Size: 1388 940
PflMI CCAn_nnn'nTGG
Cuts at: 0 906 2328
Size: 906 1422
PfoI T'CCnGG_A
Cuts at: 0 746 1607 2328
Size: 746 861 721
Fragments arranged by size:
861 746 721
PpuMI rG'GwC_Cy
Cuts at: 0 1645 1732 2241 2328
Size: 1645 87 509 87
Fragments arranged by size:
1645 509 87 87
PsiI TTA'TAA
Cuts at: 0 1756 1823 2328
Size: 1756 67 505
Fragments arranged by size:
1756 505 67
PstI C_TGCA'G
Cuts at: 0 815 2328
Size: 815 1513
PvuII CAG'CTG
Cuts at: 0 1021 1278 1626 1951 2328
Size: 1021 257 348 325 377
Fragments arranged by size:
1021 377 348 325 257
ScaI AGT'ACT
Cuts at: 0 303 834 2037 2328
Size: 303 531 1203 291
Fragments arranged by size:
1203 531 303 291
SfcI C'TryA_G
Cuts at: 0 811 2328
Size: 811 1517
SmlI C'TyrA_G
Cuts at: 0 801 2031 2328
Size: 801 1230 297
Fragments arranged by size:
1230 801 297
SspI AAT'ATT
Cuts at: 0 251 2328
Size: 251 2077
StyI C'CwwG_G
Cuts at: 0 290 905 1736 2025 2328
Size: 290 615 831 289 303
Fragments arranged by size:
831 615 303 290 289
TatI w'GTAC_w
Cuts at: 0 301 832 1945 2035 2328
Size: 301 531 1113 90 293
Fragments arranged by size:
1113 531 301 293 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1490 1522 2328
Size: 1490 32 806
Fragments arranged by size:
1490 806 32
Tth111I GACn'n_nGTC
Cuts at: 0 2170 2328
Size: 2170 158
XmnI GAAnn'nnTTC
Cuts at: 0 1304 2046 2328
Size: 1304 742 282
Fragments arranged by size:
1304 742 282
Enzymes that do cut:
AccI Acc65I AcuI AflII AlwNI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BciVI BclI BglII BmrI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BssSI BstYI Bsu36I BtgI CspCI DraI EaeI EarI
Eco57MI EcoO109I Hin4I HincII KpnI MmeI MslI MspA1I
NcoI NdeI NspI PflMI PfoI PpuMI PsiI PstI
PvuII ScaI SfcI SmlI SspI StyI TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI BsaAI BsaHI BseYI BsiEI BsiWI BsmBI
BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII XbaI XcmI XhoI
XmaI ZraI