Restriction map for
pFN21AE0423
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MspA1I MunI NaeI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 583 CGCG CG^CG
AlwNI 262 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 170 TGGCCA TGG^CCA
BbeI 245 GGCGCC GGCGC^C
BbrPI 508 CACGTG CAC^GTG
BcnI 345 CCGGG CC^[GC]GG
BpmI 484 CTGGAG CTGGAG(16/14)
BsaAI 508 CACGTG [CT]AC^GT[GA]
BsaI 649 GAGACC GGTCTC(1/5)
BsgI 355 CTGCAC GTGCAG(16/14)
BsrFI 242 ACCGGC [GA]^CCGG[CT]
BstAPI 507 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 6 CATCC GGATG(2/0)
BstUI 583 CGCG CG^CG
CfoI 246 GCGC GCG^C
Cfr10I 242 ACCGGC [GA]^CCGG[CT]
DpnII 387 GATC ^GATC
DraIII 508 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 633 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 444 GCGGG CCCGC(4/6)
FokI 6 CATCC GGATG(9/13)
HaeII 245 GGCGCC [GA]GCGC^[CT]
HgaI 427 GACGC GACGC(5/10)
HhaI 246 GCGC GCG^C
HinP1I 246 GCGC G^CGC
HinfI 694 GACTC G^A[ACGT]TC
KasI 245 GGCGCC G^GCGCC
MaeII 509 ACGT A^CGT
MaeIII 341 GTAAC ^GT[ACGT]AC
MboI 387 GATC ^GATC
MluNI 170 TGGCCA TGG^CCA
MscI 170 TGGCCA TGG^CCA
MslI 453 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 583 CGCG CG^CG
NarI 245 GGCGCC GG^CGCC
NciI 345 CCGGG CC^[GC]GG
NdeII 387 GATC ^GATC
NruI 582 TCGCGA TCG^CGA
PaeR7I 284 CTCGAG C^TCGAG
PleI 694 GACTC GAGTC(4/5)
PmaCI 508 CACGTG CAC^GTG
PmlI 508 CACGTG CAC^GTG
SacI 564 GAGCTC GAGCT^C
Sau3AI 387 GATC ^GATC
SfoI 245 GGCGCC GGC^GCC
SgrAI 241 CACCGGCG C[GA]^CCGG[CT]G
SmlI 284 CTCGAG C^T[CT][GA]AG
Tsp509I 553 AATT ^AATT
XhoI 284 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 245 GGCGCC G[GA]^CG[CT]C
427 GACGCC
AspHI 564 GAGCTC G[AT]GC[AT]^C
660 GAGCAC
AvaI 67 CCCGAG C^[CT]CG[GA]G
284 CTCGAG
BanII 400 GAGCCC G[GA]GC[CT]^C
564 GAGCTC
BbvI 42 GCAGC GCAGC(8/12)
601 GCTGC
BsaHI 245 GGCGCC G[GA]^CG[CT]C
427 GACGCC
BseRI 55 CTCCTC GAGGAG(10/8)
586 GAGGAG
BsiHKAI 564 GAGCTC G[AT]GC[AT]^C
660 GAGCAC
BsmAI 649 GAGAC GTCTC(1/5)
319 GTCTC
BsmFI 446 GGGAC GGGAC(10/14)
620 GGGAC
BsoBI 67 CCCGAG C^[CT]CG[GA]G
284 CTCGAG
BsrDI 129 CATTGC GCAATG(2/0)
11 GCAATG
Cac8I 62 GCCTGC GC[ACGT]^[ACGT]GC
503 GCAGGC
DsaI 99 CCATGG C^C[GA][CT]GG
204 CCATGG
EaeI 170 TGGCCA [CT]^GGCC[GA]
461 TGGCCG
EarI 181 GAAGAG CTCTTC(1/4)
336 GAAGAG
EcoT14I 99 CCATGG C^C[AT][AT]GG
204 CCATGG
HapII 243 CCGG C^CGG
345 CCGG
Hin1I 245 GGCGCC G[GA]^CG[CT]C
427 GACGCC
HpaII 243 CCGG C^CGG
345 CCGG
HphI 215 TCACC GGTGA(8/7)
556 TCACC
Ksp632I 181 GAAGAG CTCTTC(1/4)
336 GAAGAG
MspI 243 CCGG C^CGG
345 CCGG
NcoI 99 CCATGG C^CATGG
204 CCATGG
NspI 38 ACATGC [GA]CATG^[CT]
327 GCATGT
StyI 99 CCATGG C^C[AT][AT]GG
204 CCATGG
TaqI 285 TCGA T^CGA
527 TCGA
TseI 42 GCAGC G^C[AT]GC
601 GCTGC
TspRI 46 CACTG CA[GC]TG[ACGT][ACGT]^
390 CAGTG
TthHB8I 285 TCGA T^CGA
527 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AvaII BanI BbvCI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII ItaI
MboII MnlI MvaI MwoI NlaIII
NlaIV PpuMI Sau96I ScrFI StuI